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Sasaki, M., Takagi, A., Ota, S., Kawano, S., Sasaki, D. and Asayama, M.,
Coproduction of lipids and extracellular polysaccharides from the novel green alga Parachlorella sp. BX1.5 depending on cultivation conditions. Biotechnology Reports. 25: e00392 (2020) DOI: https://doi.org/10.1016/j.btre.2019.e00392
Azis, H. R., Etteieb, S., Takahashi, S., Koshiyama, M., Fujisawa, H. and Isoda, H.,
Effect of prohydrojasmon on total phenolic content, anthocyanin accumulation and antioxidant activity in Komatsuna and Lettuce. Bioscience, Biotechnology and Biochemistry. 84(1):178-186 (2020) DOI: https://doi.org/10.1080/09168451.2019.1673146
2019
Ota, S., Oshima, K., Yamazaki, T., Takeshita, T., Bišová, K., Zachleder, V., Hattori, M. and Kawano, S.,
The Parachlorella genome and transcriptome endorse active RWP-RK, meiosis and flagellar
genes in trebouxiophycean algae. Cytologia84(4):323-330 (2019) DOI: https://doi.org/10.1508/cytologia.84.323
Lindley, M., Hiramatsu, K., Nomoto, H., Shibata, F., Takeshita, T., Kawano, S. and Goda, K.,
Ultrafast simultaneous Raman-fluorescence spectroscopy. Anal. Chem.91(24):15563–15569 (2019) DOI: https://doi.org/10.1021/acs.analchem.9b03563
Ichihara, K., Yamazaki, T., Miyamura, S., Hiraoka, M. and Kawano, S.,
Asexual thalli originated from sporophytic thalli via apomeiosis in the green seaweed Ulva Scientific Reports9, 13523 (2019) DOI: https://doi.org/10.1038/s41598-019-50070-x
Ohnuki, S., Kashima, M., Yamada, T., Ghanegolmohammadi, F., Zhou, Y., Goshima, T.,
Maruyama, J., Kitamoto, K., Hirata, D., Akao, T. and Ohya, Y.,
Genome editing to generate nonfoam-forming sake yeast strains. Bioscience, Biotechnology and Biochemistry83(8):1583-1593 (2019) DOI: https://doi.org/10.1080/09168451.2019.1631146
Ohya, Y. and Kashima, M.,
History, lineage and phenotypic differentiation of sake yeast. Bioscience, Biotechnology and Biochemistry83(8):1442-1448 (2019) DOI: https://doi.org/10.1080/09168451.2018.1564620
Takeshita, T., Oshima, K., Ishii, K., Kawamoto, H., Ota, S., Yamazaki, T., Hirata, A., Kazama, Y., Abe, T., Hattori, M. and Kawano, S.,
Increase of lipid production upon outdoor cultivation of heavy-ion beam irradiation mutant Parachlorella kessleri PK4 and identification of its genetic variations. RIKEN Accel. Prog. Rep. 52, 218 (2019)
Kawano, Y., Shiroyama, M., Kanazawa, K., Suzuki, Y. and Ohtsu, I.,
Development of high-throughput quantitative analytical method for L-cysteine-containing dipeptides by LC-MS/MS toward its fermentative production. AMB Express9, 91(2019) DOI: https://doi.org/10.1186/s13568-019-0817-2
Yamada, K., Nitta, T., Atsuji, K., Shiroyama, M., Inoue, K., Higuchi, C., Nitta, N., Oshiro, S., Mochida, K., Iwata, O., Ohtsu, I. and Suzuki, K.,
Characterization of sulfur-compound metabolism underlying wax-ester fermentation
in Euglena gracilis. Scientific Reports 9, 853 (2019) DOIi: https://doi.org/10.1038/s41598-018-36600-z 株式会社ユーグレナのニュースリリースへ
Tanaka, N., Hatano, T., Saito, S., Wakabayashi, Y., Abe, T., Kawano, Y. and Ohtsu, I.,
Generation of hydrogen sulfide from sulfur assimilation in Escherichia coli. J Gen Appl Microbiol. 65(5): 234-239(2019) DOI: https://doi.org/10.2323/jgam.2018.11.001
Tanaka, N., Kawano, Y., Satoh, Y., Dairi, T. and Ohtsu, I.,
Gram-scale fermentative production of ergothioneine driven by overproduction
of cysteine in Escherichia coli. Scientific Reports9, 1895 (2019) DOI: https://doi.org/10.1038/s41598-018-38382-w
Takahashi, S., Yoshida, M., Watanabe, M. and Isoda, H.,
Anti-Inflammatory Effects of Aurantiochytrium limacinum 4W-1b Ethanol Extract on Murine Macrophage RAW264 Cells. BioMed Research International Volume 2019, Article ID 3104057 DOI: https://doi.org/10.1155/2019/3104057
Ota, S. and Kawano, S.,
Three-dimensional ultrastructure and hyperspectral imaging of metabolite accumulation and dynamics in Haematococcus and Chlorella. Microscopy , 68, 57-68 (2019) DOIi: https://doi.org/10.1093/jmicro/dfy142
Kubo, K., Okada, H., Shimamoto, T., Kimori, Y., Mizunuma, M., Bi, E., Ohnuki, S. and Ohya, Y.,
Implications of maintenance of mother-bud neck size in diverse vital processes of Saccharomyces cerevisiae. Curr Genet. 65(1):253-267. (2019) DOI: https://doi.org/10.1007/s00294-018-0872-2
Nemoto, S., Ohnuki, S., Abe, F. and Ohya, Y.,
Simulated microgravity triggers characteristic morphology and stress responsein Saccharomyces cerevisiae. Yeast. 36(2):85-97. (2019) DOI: https://doi.org/10.1002/yea.3361
2018
Takahashi, S., Sakamaki, M., Ferdousi, F., Yoshida, M., Demura, M., Watanabe, M. and
Isoda, H.,
Ethanol Extract of Aurantiochytrium mangrovei 18W-13a Strain Possesses Anti-inflammatory Effects on Murine Macrophage RAW264 Cells. Front Physiol. 9, 1205. (2018) DOI: https://doi.org/10.3389/fphys.2018.01205
Furukawa, M., Sato, T., Suzuki, Y., Casareto, B. E. and Hirabayashi, S.,
Numerical modelling of physiological and ecological impacts of ocean acidification on Coccolithophores. J. Marine Syst. 182, 12–30. (2018) DOI: https://doi.org/10.1016/j.jmarsys.2018.02.008
Yamazaki, T., Konosu, E., Takeshita, T., Hirata, A., Ota, S., Kazama,Y., Abe, T. and Kawano, S.,
Independent regulation of the lipid and starchsynthesis pathways by sulfate
metabolites in the green microalga Parachlorella kessleri under sulfur starvation conditions. Algal Research36, 37–47 (2018) DOI: https://doi.org/10.1016/j.algal.2018.09.022
Takeshita, T., Ivanov, I. N., Oshima, K., Ishii, K., Kawamoto, H., Ota,S., Yamazaki,
T., Hirata, A., Kazama, Y., Abe, T., Hattori, M., Bišováb,K., Zachleder,
V. and Kawano, S.,
Comparison of lipid productivity of Parachlorella kessleri heavy-ion beam irradiation mutant PK4 in laboratory and 150-L mass bioreactor,
identification and characterization of its genetic variation. Algal Research35, 416–426 (2018) DOI: https://doi.org/10.1016/j.algal.2018.09.005
Yoon, J., Kanamori, A., Fujii, K. and Isoda, H.,
Evaluation of maslinic acid with whole-body vibration training in elderly women with knee osteoarthritis. PLOS One13(3) (2018) DOI: https://doi.org/10.1371/journal.pone.0194572
Suzuki, G., Wang, Y., Kubo, K., Hirata, E., Ohnuki, S. and Ohya, Y.,
Global study of holistic morphological effectors in the budding yeast Saccharomyces cerevisiae. BMC Genomics.19, 149. (2018) DOI: https://doi.org/10.1186/s12864-018-4526-z
Ohnuki, S. and Ohya, Y.,
High-dimensional single-cell phenotyping reveals extensive haploinsufficiency. PLOS Biology 16,e2005130 (2018) DOI: https://doi.org/10.1371/journal.pbio.2005130 【解説】遺伝子の機能欠損は多くの場合劣性遺伝すると考えられてきたが、出芽酵母の必須遺伝子欠損株の細胞形態を網羅的に調べた結果、約6割の遺伝子の機能欠損が逆に優性遺伝することを発見した。ヘテロ接合型で遺伝子欠損株が示した表現型は、遺伝子機能に密接に関係していることがわかったので、研究開発課題4(機能性試験と新規成分抽出法の確立と機能性素材の開発)において、薬剤標的の探索を行っていく際に新しい標的機能性素材の開発に資することが期待される。
Sukegawa, Y., Negishi, T., Kikuchi, Y., Ishii, K., Imanari, M., Ghanegolmohammadi,
F., Nogami, S. and Ohya, Y.,
Genetic dissection of the signaling pathway required for the cell wall integrity checkpoint. Journal of Cell Science jcs.219063 (2018) DOI: https://doi.org/10.1242/jcs.219063
Ota, S., Morita, A., Ohnuki, S., Hirata, A., Sekida, S., Okuda, K., Ohya, Y. and Kawano, S.,
Carotenoid dynamics and lipid droplet containing astaxanthin in response to light in the green alga Haematococcus pluvialis Scientific Reports 8, 5617 (2018) DOI: https://doi.org/10.1038/s41598-018-23854-w 【解説】ヘマトコッカス藻のカロテノイドのなかで、細胞膜の直下に移動して日焼け止めのシェードになるのはアスタキサンチンだけであることを、ハイパースペクトルカメラを使って明らかにした。アスタキサンチンは脂溶性なので、アスタキサンチンが細胞膜の直下に移動するということは油滴が細胞膜の直下で葉緑体を覆うようになる。カロテノイドは微細藻類が生産する機能性物質の1つでこうした細胞応答の解析は生産性の向上にもつながる。
2017
Yasuda, T., Kamahori, M., Nagata, K., Watanabe-Asaka, T., Suzuki, M., Funayama,
T., Mitani, H. and Oda, S.,
Abscopal activation of microglia in embryonic fish brain following targeted
irradiation with heavy-ion microbeam. Int J Mol Sci 18(7): E1428. (2017) DOI: https://doi.org/10.3390/ijms18071428
Ho, W.C., Ohya, Y. and Zhang, J.,
Testing the neutral hypothesis of phenotypic evolution. Proc Natl Acad Sci U S A. 114, 12219-12224. (2017) DOI: https://doi.org/10.1073/pnas.1710351114
Kawaoka, T., Ohnuki, S., Ohya, Y. and Suzuki, K.,
Morphometric analysis of autophagy-related structures in Saccharomyces cerevisiae. Autophagy, 13, 2104-2110 (2017) DOI: https://doi.org/10.1080/15548627.2017.1384888
Hirata, E., Ohya, Y. and Suzuki, K.,
Atg4 plays an important role in efficient expansion of autophagic isolation
membranes by cleaving lipidated Atg8 in Saccharomyces cerevisiae. PLOS ONE, 12, e0181047 (2017) DOI: https://doi.org/10.1371/journal.pone.0181047
Ghanegolmohammadi, F., Yoshida, M., Ohnuki, S., Sukegawa, Y., Okada, H., Obara, K., Kihara, A., Suzuki, K., Kojima, T., Yachie, N., Hirata, D. and Ohya, Y.,
Systematic analysis of Ca2+ homeostasis in Saccharomyces cerevisiae based on chemical-genetic interaction profiles. Mol Biol Cell. 28, 3415-3427 (2017) DOI: https://doi.org/10.1091/mbc.E17-04-0216
Matsukawa, T., Motojima, H., Sato, Y., Takahashi, S. and Isoda, H.,
Upregulation of skeletal muscle PGC-1α through the elevation of cyclic AMP levels by Cyanidin-3-glucoside enhances exercise performance. Scientific Reports 7, 44799 (2017) DOI: https://doi.org/10.1038/srep44799
Kawasumi, R., Narita, S., Miyamoto, K., Tominaga, K., Takita, R., and Uchiyama, M.,
One-step Conversion of Levulinic Acid to Succinic Acid Using I2 /t-BuOK System: The Iodoform Reaction Revisited. Scientific Reports7, 17967 (2017) DOI: https://doi.org/10.1038/s41598-017-17116-4
Yamazaki, T., Ichihara, K., Suzuki, R., Oshima, K., Miyamura, S., Kuwano, K., Toyoda, A., Suzuki, Y., Sugano, S., Hattori, M. and Kawano, S.,
Genomic structure and evolution of the mating type locus in the green seaweed Ulva partita. Scientific Reports7, 11679 (2017) DOI: https://doi.org/10.1038/s41598-017-11677-0
Hu, J-Y. and Sato, T.,
A photobioreactor for microalgae cultivation with internal illumination considering flashing light effect and optimized light-source arrangement. Energy Conv. Mangmnt. 133,558–565(2017) DOI: https://doi.org/10.1016/j.enconman.2016.11.008 【解説】LEDを光源としたフォトバイオリアクターの試作機を設計し、作成した。試作機で培養実験を行い、培養の際もFlashing Light 照射により、増殖が連続光より速くなり、最高濃度が高くなることが確認できた。
Akizuki, M. and Oshima, Y.,
Effect of water properties on selectivity for 1-octene and 2-octanol reaction systems in sub- and supercritical water using a TiO2 catalyst. J. Supercritical Fluids 123, 76 (2017) DOI: https://doi.org/10.1016/j.supflu.2016.11.018 【解説】超臨界水と二酸化チタン触媒を組み合わせた反応場において、温度と圧力に伴う超臨界水の物性変化が、触媒表面で進行するオレフィンやアルコールの酸触媒反応の速度と生成物選択性に大きく影響することを明らかにし、そのモデル化を通じて温度と圧力をパラメータとした反応制御の可能性を明らかにした。
Akizuki, M. and Oshima, Y.,
Isomerization of α-Pinene to monocyclic monoterpenes in hot compressed water using TiO2 and WOx/TiO2 catalysts Ind. Eng. Chem. Res., 56, 6204–6212 (2017) DOI: https://doi.org/10.1021/acs.iecr.7b01172 【解説】高温高圧水を溶媒とし、再生可能資源であるピネンのモノテルペン類への変換反応を固体酸触媒を利用して検討し、高温高圧水が持つ高いプロトン供与性が固体触媒の表面状態に影響することで、単環式のモノテルペン類への選択的な異性化反応が進行することを明らかにした。
2016
Ota, S., Oshima, K., Yamazaki, T., Kim, S., Yu, Z., Yoshihara, M., Takeda,
K., Takeshita,T., Hirata, A., Bišova, K., Zachleder, V., Hattori, M., and
Kawano, S.
Highly efficient lipid production in the green alga Parachlorella kessleri: draft genome and transcriptome endorsed by whole-cell 3D ultrastructure.
Biotechnol. Biofuels. 9, 13 (2016) DOI: https://doi.org/10.1186/s13068-016-0424-2
Nagata, K., Hashimoto, C., Watanabe-Asaka, T., K. Itoh, Yasuda, T., Ohta, K., Oonishi, H., Igarashi, K., Suzuki, M., Funayama, T., Kobayashi, Y., Nishimaki, T., Katsumura, T., Oota, H., Ogawa, M., Oga, A., Ikemoto, K., Itoh, H., Kutsuna, N., Oda, S. and Mitani, H., In vivo 3D analysis of systemic effects after local heavy-ion beam irradiation in an animal model. Scientific Reports6: 28691 (2016) DOI: https://doi.org/10.1038/srep28691
Ota, S., Yoshihara, M., Yamazaki, T., Takeshita, T., Hirata, A., Konomi, M., Oshima, K., Hattori., M., Bišová, K., Zachleder, V. and Kawano, S.,
Deciphering the relationship among phosphate dynamics, electron-dense body
and lipid accumulation in the green alga Parachlorella kessleri. Scientific Reports6, 25731 (2016) DOI: https://doi.org/10.1038/srep25731
Yamasaki, A., Watanabe, Y., Adachi, Y., Suzuki, K., Matoba, K., Kirisako, H., Kumeta, H., Nakatogawa, H., Ohsumi, Y., Inagaki, F.
and Noda, N.,
Structural basis for receptor-mediated selective autophagy of aminopeptidase I aggregates. Cell Rep. 16, 19-27 (2016) DOI: https://doi.org/10.1016/j.celrep.2016.05.066
Kawachi, M., Kataoka, T., Sato, M., Noel, M-H., Kuwata, A., Demura M. and Yamaguchi.
H.,
Application of cryopreservation to genetic analyses of a photosynthetic picoeukaryote community. Gene, 576 (2), 708-716 (2016) DOI: https://doi.org/10.1016/j.gene.2015.10.033
Tominaga, K., Nemoto, K., Kamimura, Y., Yamada, A., Yamamoto, Y. and Sato, K.,
A practical and efficient synthesis of methyl levulinate from cellulosic biomass catalyzed by an aluminum-based mixed acid catalyst system. RSC Advances, 6, 65119 (2016) DOI: https://doi.org/10.1039/C6RA15638J 【解説】レブリン酸はブテンなどの基礎化学品やアジピン酸やコハク酸などのエンブラモノマーに変換可能であり、バイオマスリファイナリーにおける重要な基幹物質である。本論文では、安価なアルミニウム化合物とスルホン酸化合物を組み合わせた触媒系を用いることにより、木粉等の含セルロース原料から高濃度で収率よくレブリン酸メチルを合成可能なことを示した。
2015
Hirama, H. and Torii, T.,
One-to-one encapsulation based on alternating droplet generation. Scientific Reports, 5, 15196 (2015) DOI: https://doi.org/10.1038/srep15196
Murata, Y., Yasuda, T., Watanabe-Asaka, T., Oda, S., Mantoku, A., Takeyama, K., Chatani, M., Kudo, A., Uchida, S., Suzuki,
H., Tanigaki, F., Shirakawa, M., Fujisawa, K., Hamamoto, Y., Terai, S.
and Mitani, H.,
Histological and Transcriptomic Analysis of Adult Japanese Medaka Sampled Onboard the International Space Station. PLoS One10 (10) e0138799 (2015) DOI: https://doi.org/10.1371/journal.pone.0138799
Piotrowski, J.S., Okada, H., Lu, F., Li, S.C., Hinchman, L., Ranjan, A.,
Smith, D.L., Higbee, A.J., Ulbrich, A., Coon, J.J., Deshpande, R., Bukhman,
Y.V., McIlwain, S., Ong, I.M., Myers, C.L., Boone, C., Landick, R., Ralph,
J., Kabbage, M. and Ohya, Y.,
Plant-derived antifungal agent poacic acid targets β-1,3-glucan. Proc Natl Acad Sci U S A112, E1490-7 (2015) DOI: https://doi.org/10.1073/pnas.1410400112 【解説】微機能性試験と形態プロファイリングにおいて、鍵を握るのが標的未知物質の細胞内標的の同定である。トウモロコシの茎の加水産物として得られたポアシン酸はそれまで作用機構未知の抗真菌剤であったが、この研究では形態プロファイリングという新しい技術を使い、細胞壁合成を阻害することを突き止め、特に細胞壁の1,3-ß-グルカンに結合することを始めて証明した。
Komazaki, Y., Fuchigami, K. and Torii, T.,
Rapid Prototyping of a Flow-Focusing Device for Monodisperse Droplet Formation Using Glass Capillary and Pipette Tip. Proceedings of ISMM, PM-17 (2015) 【解説】ガラスキャピラリーを入れ子構造にすることでフローフォーカシングデバイスを実現し、直径の揃った液滴生成が可能であることを示した。
Fuchigami, K., Komazaki, Y. and Torii, T.,
A Synthesis of Mono-dispersed Matrix Functional Composite Microparticles Using a Flow-focusing Device. Proceedings of ISMM, PM-08 (2015) 【解説】歯科材料用途として固体微粒子を含む液滴をガラスキャピラリーを用いたフローフォーカシングデバイスで生成したところ、閉塞無しに生成が可能であり、これまで実現できなかった固体微粒子含有量まで実現することができた。