clone 153

Clone number	clone 153
Clone name (database links)	yk578a1 (EST database) (NEXTDB) (Sanger Centre)
cDNA group (database links)	CELK07556 (EST database) (NEXTDB)
Cosmid or YAC	C56A3
CDS identification number	C56A3.8
database links	(Wormpep)	(WormBase)	(Worm PD)
BLAST search results	BLASTP against nr-aa	TBLASTN against human EST	TBLASTN against mouse EST
Homologues or conserved domains
*No. of C. elegans* paralogues (E<1e-50)**	1
Differential hybridization	Spot address on high-density grid	Signal intensity for F cDNA probe	Signal intensity for R cDNA probe
Differential hybridization	021P14	3.134	-
RNAi phenotype	Sterile, Larval lethal
embryonic viability (of F1)	76.8 %
sterility (laid no eggs)	50% (4 out of 8 F1 animals)
sterility (laid more than 5 unfertilized eggs)	0% (0 out of 8 F1 animals)

clone number	153
clone name	yk578a1
clone family	CELK 07556
gene name	C56A3.8
homology	-
motif	-
in situ hybridization
RNAi	Glp phenotype, Larval lethal

in situ hybridization:

RNAi: Glp, Gld, Gon

All of the F1 worms were arrested at L1 stage or sterile.

Data (F1)
No. of unfertilized eggs No. of eggs No. of hatched eggs Hatching rate (%)

0 0 0 -

0 24 24 100

0 37 34 91.9

0 0 0 -

0 0 0 -

0 0 0 -

0 41 39 95.1

7 8 1 100

Data (F1)
No. of unfertilized eggs	No. of eggs	No. of hatched eggs	Hatching rate (%)
0	0	0	-
0	24	24	100
0	37	34	91.9
0	0	0	-
0	0	0	-
0	0	0	-
0	41	39	95.1
7	8	1	100

Photos :

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