Clone number | |||
Clone name (database links) |
(EST database) (NEXTDB) (Sanger Centre) |
||
cDNA group (database links) |
(EST database) (NEXTDB) |
||
Cosmid or YAC | |||
CDS identification number | |||
database links |
|||
BLAST search results |
|||
Homologues or conserved domains | |||
No. of C. elegans paralogues (E<1e-50) | |||
Differential hybridization | high-density grid |
F cDNA probe |
R cDNA probe |
RNAi phenotype | |||
embryonic viability (of F1) |
|||
sterility (laid no eggs) |
|||
sterility (laid more than 5 unfertilized eggs) |
clone number | 10 |
clone name | yk437b3 |
clone family | CELK 05644 |
gene name | C25G4.6 |
homology | novel |
motif | PDZ domain |
in situ hybridization | L4 germline |
RNAi | Spermatogenesis defects |
in situ hybridization:Staining
was observed during L4 in hermaphrodite and at all stage in male, when
the spermatogenesis occured.
|
|
|
|
|
RNAi:Hermaphrodites did not have
sperm and laid eggs with apperarance similar to un fertilized eggs. Males
did not show any defects.
No. of unfertilized eggs | No. of eggs | No. of hatched eggs | Hatching rate (%) |
7 | 7 | 0 | - |
16 | 16 | 0 | - |
31 | 31 | 0 | - |
0 | 29 | 28 | 96.6 |
No. of unfertilized eggs | No. of eggs | No. of hatched eggs | Hatching rate (%) |
9 | 91 | 81 | 98.8 |
25 | 114 | 89 | 100 |
13 | 83 | 60 | 85.7 |
94.8»}7.9 |
No. of unfertilized eggs | No. of eggs | No. of hatched eggs | Hatching rate (%) |
many | |||
many | |||
many | |||
many | |||
many | |||
many | |||
many | |||
many | |||
many | |||
many | |||
4 | 33 | 19 | 65.5 |
8 | 27 | 0 | 0 |
18 | 39 | 19 | 90.5 |
12 | 28 | 8 | 50 |
7 | 26 | 16 | 84.2 |
No. of unfertilized eggs | No. of eggs | No. of hatched eggs | Hatching rate (%) |
2 | 40 | N. D. | N. D. |
18 | 104 | N. D. | N. D. |
6 | 101 | N. D. | N. D. |
Photos
|
|
|
|||
|
before cross with N2 male
|
after cross with N2 male
|
|||
|
|
|
|||