clone 10

Clone number
clone 10
Clone name
 (database links)
yk437b3
(EST database) (NEXTDB) (Sanger Centre)
cDNA group
 (database links)
CELK05644
(EST database) (NEXTDB)
Cosmid or YAC
C25G4
CDS identification number
C25G4.6
database links
(Wormpep)
(WormBase)
(Worm PD)
BLAST search results
BLASTP against nr-aa
TBLASTN against human EST
TBLASTN against mouse EST
Homologues or conserved domains
PDZ domain (Also known as DHR or GLGF). (2 domains)
No. of C. elegans paralogues (E<1e-50)
2
Differential hybridization
Spot address on
high-density grid
Signal intensity for
F cDNA probe
Signal intensity for
R cDNA probe
011B09
18.636
0.494
RNAi phenotype
Sterile
embryonic viability (of F1)
96.2 %
sterility (laid no eggs)
0% (0 out of 8 F1 animals)
sterility (laid more than 5 unfertilized eggs)
100% (8 out of 8 F1 animals)
clone    number 10
clone name yk437b3
clone family CELK 05644
gene name C25G4.6
homology novel
motif PDZ domain
in situ hybridization L4 germline 
RNAi Spermatogenesis defects

in situ hybridization:Staining was observed during L4 in hermaphrodite and at all stage in male, when the spermatogenesis occured.
 

RNAi:Hermaphrodites did not have sperm and laid eggs with apperarance similar to un fertilized eggs. Males did not show any defects.
 
Before cross (F1)
No. of unfertilized eggs No. of eggs No. of hatched eggs Hatching rate (%)
7 7 0 -
16 16 0 -
31 31 0 -
0 29 28 96.6
After cross (F1)
No. of unfertilized eggs No. of eggs No. of hatched eggs Hatching rate (%)
9 91 81 98.8
25 114 89 100
13 83 60 85.7
94.8»}7.9
Before cross (F1)
No. of unfertilized eggs No. of eggs No. of hatched eggs Hatching rate (%)
many
many
many
many
many
many
many
many
many
many
4 33 19 65.5
8 27 0 0
18 39 19 90.5
12 28 8 50
7 26 16 84.2
After cross (F1)
No. of unfertilized eggs No. of eggs No. of hatched eggs Hatching rate (%)
2 40 N. D. N. D.
18 104 N. D. N. D.
6 101 N. D. N. D.

Photos

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before cross with N2 male

after cross with N2 male