Score E
Sequences producing significant alignments: (bits) Value
gb|AW961969.1|AW961969 EST374042 MAGE resequences, MAGG Hom... 36 0.40
gb|AI806483.1|AI806483 wf08f01.x1 Soares_NFL_T_GBC_S1 Homo ... 35 1.2
gb|AI147946.1|AI147946 qg62a09.s1 Soares_testis_NHT Homo sa... 34 2.0
gb|AW969835.1|AW969835 EST381913 MAGE resequences, MAGK Hom... 34 2.7
gb|AA779449.1|AA779449 af22d02.s1 Soares_total_fetus_Nb2HF8... 34 2.7
gb|AI341843.1|AI341843 qt10a09.x1 NCI_CGAP_GC4 Homo sapiens... 32 6.0
gb|AW590961.1|AW590961 hg51g06.x1 NCI_CGAP_GC6 Homo sapiens... 32 7.8
gb|AI479601.1|AI479601 tm32a02.x1 NCI_CGAP_CLL1 Homo sapien... 32 7.8
gb|AI150906.1|AI150906 qb48g10.x1 NCI_CGAP_Brn23 Homo sapie... 32 7.8
gb|AA483733.1|AA483733 ne91a06.s1 NCI_CGAP_Kid1 Homo sapien... 32 7.8
gb|AA483203.1|AA483203 ne87e01.s1 NCI_CGAP_Kid1 Homo sapien... 32 7.8
Alignments
>gb|AW961969.1|AW961969 EST374042 MAGE resequences, MAGG Homo sapiens cDNA.
Length = 620
Score = 36.4 bits (82), Expect = 0.40
Identities = 29/107 (27%), Positives = 51/107 (47%), Gaps = 19/107 (17%)
Frame = +2
Query: 134 VLVSRVDPGSLAEKC--LVLGDHLCDVDGIPVSDKDVARDLLVKNIQEKGKVTFVVERPD 191
+ V V+P S+A K + GD + ++G+ V +++ A +L + +E ++ +V RP+
Sbjct: 47 IYVGEVNPNSIAAKDGRIREGDRIIQINGVDVQNREEAVAILSQ--EENTNISLLVARPE 220
Query: 192 SIDAKQWAK----------------QALATNLMQPPSVQM-NEDVKG 221
S AK+W + A L PP+ Q NE+ KG
Sbjct: 221 SQLAKRWKDSDRDDFLDDFGSENEGELRARKLKSPPAQQPGNEEEKG 361
>gb|AI806483.1|AI806483 wf08f01.x1 Soares_NFL_T_GBC_S1 Homo sapiens cDNA clone
IMAGE:2350009 3' similar to SW:MPP2_HUMAN Q14168 MAGUK
P55 SUBFAMILY MEMBER 2 ;.
Length = 663
Score = 34.8 bits (78), Expect = 1.2
Identities = 26/91 (28%), Positives = 43/91 (46%), Gaps = 9/91 (9%)
Frame = -1
Query: 11 IDMEEGEPLGAT---PNDKLVITKIQAGTI--SEGKLRIGDQVKKVN----GQNCKDCND 61
I GEPLG T N+ LVI +I G + +G L +GD +K+VN G N K+ +
Sbjct: 516 IHKRAGEPLGVTFRVENNDLVIARILHGGMIDRQGLLHVGDIIKEVNGHEVGNNPKELQE 337
Query: 62 FFRALRFAAPCAKIMVNRDEKKAEELEARVH 92
+ + + + RD +++ + H
Sbjct: 336 LLKNISGSVTLKILPSYRDTITPQQVFVKCH 244
>gb|AI147946.1|AI147946 qg62a09.s1 Soares_testis_NHT Homo sapiens cDNA clone IMAGE:1839736
3' similar to SW:DLG2_HUMAN Q14168 MPP2 PROTEIN ;.
Length = 565
Score = 34.0 bits (76), Expect = 2.0
Identities = 20/43 (46%), Positives = 27/43 (62%), Gaps = 5/43 (11%)
Frame = -1
Query: 16 GEPLGAT---PNDKLVITKIQAGTI--SEGKLRIGDQVKKVNG 53
GEPLG T N+ LVI +I G + +G L +GD +K+VNG
Sbjct: 499 GEPLGVTFRVENNDLVIARILHGGMIDRQGLLHVGDIIKEVNG 371
>gb|AW969835.1|AW969835 EST381913 MAGE resequences, MAGK Homo sapiens cDNA.
Length = 402
Score = 33.6 bits (75), Expect = 2.7
Identities = 17/47 (36%), Positives = 23/47 (48%)
Frame = +3
Query: 37 ISEGKLRIGDQVKKVNGQNCKDCNDFFRALRFAAPCAKIMVNRDEKK 83
+S G L+ GD +K N C DC+D A RF + N +KK
Sbjct: 177 LSSGFLKFGDVRQKYNSLECLDCSDLSLAFRFDCHFRFLSKNTKKKK 317
>gb|AA779449.1|AA779449 af22d02.s1 Soares_total_fetus_Nb2HF8_9w Homo sapiens cDNA clone
IMAGE:1032387 3' similar to TR:Q16886 Q16886 MYOSIN
BINDING PROTEIN H. ;.
Length = 559
Score = 33.6 bits (75), Expect = 2.7
Identities = 26/89 (29%), Positives = 41/89 (45%), Gaps = 1/89 (1%)
Frame = -2
Query: 140 DPGSLAEKCLVLGDHLCD-VDGIPVSDKDVARDLLVKNIQEKGKVTFVVERPDSIDAKQW 198
DP AE CL DHL + DG P + K + + E+G T + +P D+ +
Sbjct: 531 DPADCAEPCLPAQDHLDEKQDGDPGNPK-------YRALSEQGVCTLEIRKPSPFDSGVY 373
Query: 199 AKQALATNLMQPPSVQMNEDVKGIASQYR 227
+A+ N++ SV +VK A+ R
Sbjct: 372 TCKAI--NVLGEASVDCRLEVKASAAH*R 292
>gb|AI341843.1|AI341843 qt10a09.x1 NCI_CGAP_GC4 Homo sapiens cDNA clone IMAGE:1947160 3'.
Length = 507
Score = 32.5 bits (72), Expect = 6.0
Identities = 17/47 (36%), Positives = 25/47 (53%)
Frame = -3
Query: 37 ISEGKLRIGDQVKKVNGQNCKDCNDFFRALRFAAPCAKIMVNRDEKK 83
+S G L+ GD +K N C DC+D A RF C ++++ KK
Sbjct: 136 LSSGFLKFGDVRQKYNSLECLDCSDLSLAFRF--DCHFRFLSKNTKK 2
>gb|AW590961.1|AW590961 hg51g06.x1 NCI_CGAP_GC6 Homo sapiens cDNA clone IMAGE:2949178 3'.
Length = 481
Score = 32.1 bits (71), Expect = 7.8
Identities = 14/32 (43%), Positives = 18/32 (55%)
Frame = -2
Query: 37 ISEGKLRIGDQVKKVNGQNCKDCNDFFRALRF 68
+S G L+ GD +K N C DC+D A RF
Sbjct: 138 LSSGFLKFGDVRQKYNSLECLDCSDLSLAFRF 43
>gb|AI479601.1|AI479601 tm32a02.x1 NCI_CGAP_CLL1 Homo sapiens cDNA clone IMAGE:2158250 3'.
Length = 554
Score = 32.1 bits (71), Expect = 7.8
Identities = 14/32 (43%), Positives = 18/32 (55%)
Frame = -2
Query: 37 ISEGKLRIGDQVKKVNGQNCKDCNDFFRALRF 68
+S G L+ GD +K N C DC+D A RF
Sbjct: 130 LSSGFLKFGDVRQKYNSLECLDCSDLSLAFRF 35
>gb|AI150906.1|AI150906 qb48g10.x1 NCI_CGAP_Brn23 Homo sapiens cDNA clone IMAGE:1703394 3'.
Length = 363
Score = 32.1 bits (71), Expect = 7.8
Identities = 26/90 (28%), Positives = 45/90 (49%), Gaps = 3/90 (3%)
Frame = -3
Query: 173 LVKNIQEKGKVTFVVERPDSIDAKQWAKQALATNLMQPPSVQMNEDVKGIASQYRQALPG 232
LV +IQEKG+ + P + ++W Q L T L +++ +GI + + + G
Sbjct: 286 LVPHIQEKGQ*RILP--PFYKNLQRWPPQGLNTALA-------SQEGRGIGNGWVPSKKG 134
Query: 233 LKPPAKSAMSTG---PSARRVSIVEQTQTH 259
L PP++ STG P R + ++ QT+
Sbjct: 133 LLPPSRHGKSTGVGVPPKRGPHLKKRLQTY 44
>gb|AA483733.1|AA483733 ne91a06.s1 NCI_CGAP_Kid1 Homo sapiens cDNA clone IMAGE:911602.
Length = 250
Score = 32.1 bits (71), Expect = 7.8
Identities = 14/32 (43%), Positives = 18/32 (55%)
Frame = -3
Query: 37 ISEGKLRIGDQVKKVNGQNCKDCNDFFRALRF 68
+S G L+ GD +K N C DC+D A RF
Sbjct: 140 LSSGFLKFGDVRQKYNSLECLDCSDLSLAFRF 45
Database: GenBank Human EST entries
Posted date: Jul 8, 2000 8:03 PM
Number of letters in database: 800,131,619
Number of sequences in database: 2,121,173
Lambda K H
0.316 0.135 0.388
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 246894325
Number of Sequences: 2121173
Number of extensions: 3072701
Number of successful extensions: 13687
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 13679
Number of HSP's gapped (non-prelim): 13
length of query: 274
length of database: 266,710,539
effective HSP length: 57
effective length of query: 217
effective length of database: 145,803,678
effective search space: 31639398126
effective search space used: 31639398126
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.6 bits)
S2: 70 (31.7 bits)
