Score E
Sequences producing significant alignments: (bits) Value
pir||T19466 hypothetical protein C25G4.6 - Caenorhabditis e... 555 e-157
pir||T15134 hypothetical protein T21G5.4 - Caenorhabditis e... 551 e-156
pir||T22040 hypothetical protein F40F9.3 - Caenorhabditis e... 72 5e-12
pir||T32784 hypothetical protein C50D2.3 - Caenorhabditis e... 51 1e-05
pir||T00346 hypothetical protein KIAA0583 - human (fragment... 48 1e-04
pir||T25637 hypothetical protein C46H11.6 - Caenorhabditis ... 46 4e-04
pir||T16202 hypothetical protein F28E10.4 - Caenorhabditis ... 46 5e-04
emb|CAB39895.1| (AJ133439) GRIP1 protein [Homo sapiens] 42 0.006
pir||T16122 hypothetical protein F20D6.1 - Caenorhabditis e... 42 0.006
pir||T29298 hypothetical protein C50F7.3 - Caenorhabditis e... 42 0.008
pir||T20145 hypothetical protein C52A11.4 - Caenorhabditis ... 39 0.050
emb|CAA86769.2| (Z46792) similar to lethal(1) discs large-1... 39 0.050
pir||JE0366 tight junction protein, ZO-2 - chicken >gi|3820... 39 0.050
pir||T32733 AMPA glutamate receptor interacting protein GRI... 39 0.066
sp|Q09284|YQJ3_CAEEL HYPOTHETICAL 18.2 KD PROTEIN C52A11.3 ... 38 0.086
pir||T27090 hypothetical protein Y51B9A.3 - Caenorhabditis ... 38 0.086
gb|AAC02527.2| (AF177533) tight junction protein ZO-2 isofo... 38 0.15
gb|AAD56219.2| (AF177533) tight junction protein ZO-2 isofo... 38 0.15
ref|NP_004808.1| Friedreich ataxia region gene X104 (tight ... 38 0.15
gb|AAD56218.2| (AF177533) tight junction protein ZO-2 isofo... 38 0.15
gb|AAC33121.1| (AF083892) tight junction protein ZO-2 isofo... 38 0.15
gb|AAD20387.2| (AF177533) tight junction protein ZO-2 isofo... 38 0.15
gb|AAC72274.1| (AC005954) ZO-3 [Homo sapiens] 38 0.15
gb|AAC33122.1| (AF083893) tight junction protein ZO-2 isofo... 37 0.19
gb|AAD39392.1|AF124435_1 (AF124435) p55-related MAGUK prote... 37 0.19
gb|AAC37332.1| (L27152) tight junction protein [Canis famil... 37 0.19
ref|NP_035727.1| tight junction protein 2 >gi|4406391|gb|AA... 37 0.19
gb|AAF22151.1|AF133093_6 (AF133093) putative protein at the... 37 0.25
gb|AAF74512.1| (U52111) LU1 protein [Homo sapiens] 36 0.33
dbj|BAA95968.1| (AB040877) KIAA1444 protein [Homo sapiens] 36 0.33
gb|AAF63789.1|AF199008_1 (AF199008) PALS1 [Mus musculus] 36 0.43
gb|AAD45009.1|AF161181_1 (AF161181) P55T protein [Mus muscu... 35 0.75
dbj|BAA81739.1| (AB006955) AIE-75 [Homo sapiens] 35 0.75
ref|NP_031888.1| discs, large homolog 1 (Drosophila) >gi|22... 35 0.75
dbj|BAA81740.1| (AB018687) autoimmune enteropathy-related a... 35 0.75
ref|NP_031730.1| channel-interacting PDZ domain protein >gi... 35 0.75
gb|AAF63790.1|AF199009_1 (AF199009) PALS2-alpha splice vari... 35 0.75
gb|AAF63791.1|AF199010_1 (AF199010) PALS2-beta splice varia... 35 0.98
ref|NP_005700.1| PDZ-73 protein >gi|3170200|gb|AAC18049.1| ... 35 0.98
gb|AAC18048.1| (AF039699) antigen NY-CO-37 [Homo sapiens] 35 0.98
dbj|BAA88229.1| (AB030499) DLG6 alpha [Rattus norvegicus] 35 0.98
sp|Q58486|YA86_METJA HYPOTHETICAL PROTEIN MJ1086 >gi|212866... 35 0.98
ref|NP_057531.1| MAGUK protein p55T; Protein Associated wit... 35 0.98
dbj|BAA88231.1| (AB030501) DLG6 gamma [Rattus norvegicus] 35 0.98
dbj|BAA88230.1| (AB030500) DLG6 beta [Rattus norvegicus] 35 0.98
gb|AAC61487.1| (AC005084) similar to mouse mCASK-A; similar... 34 1.3
gb|AAF50385.1| (AE003554) CG6416 gene product [alt 3] [Dros... 34 1.3
ref|NP_004078.1| discs, large (Drosophila) homolog 1 >gi|21... 34 2.2
sp|Q12959|SP97_HUMAN PRESYNAPTIC PROTEIN SAP97 (SYNAPSE-ASS... 34 2.2
gb|AAF59321.1| (AE003843) CG1783 gene product [Drosophila m... 33 2.9
Alignments
>pir||T19466 hypothetical protein C25G4.6 - Caenorhabditis elegans
emb|CAA94569.1| (Z70680) predicted using Genefinder~similar to PDZ domain (Also
known as DHR or GLGF). (2 domains)~cDNA EST EMBL:D69345
comes from this gene~cDNA EST EMBL:D70195 comes from
this gene~cDNA EST EMBL:D70732 comes from this gene~cDNA
EST yk301e7.3 comes fr>
Length = 274
Score = 555 bits (1414), Expect = e-157
Identities = 274/274 (100%), Positives = 274/274 (100%)
Query: 1 MGSEKMIEICIDMEEGEPLGATPNDKLVITKIQAGTISEGKLRIGDQVKKVNGQNCKDCN 60
MGSEKMIEICIDMEEGEPLGATPNDKLVITKIQAGTISEGKLRIGDQVKKVNGQNCKDCN
Sbjct: 1 MGSEKMIEICIDMEEGEPLGATPNDKLVITKIQAGTISEGKLRIGDQVKKVNGQNCKDCN 60
Query: 61 DFFRALRFAAPCAKIMVNRDEKKAEELEARVHIPEDRAKIIQRREGYVYELATLVWVQNG 120
DFFRALRFAAPCAKIMVNRDEKKAEELEARVHIPEDRAKIIQRREGYVYELATLVWVQNG
Sbjct: 61 DFFRALRFAAPCAKIMVNRDEKKAEELEARVHIPEDRAKIIQRREGYVYELATLVWVQNG 120
Query: 121 PKLGLGIKHFQNRVLVSRVDPGSLAEKCLVLGDHLCDVDGIPVSDKDVARDLLVKNIQEK 180
PKLGLGIKHFQNRVLVSRVDPGSLAEKCLVLGDHLCDVDGIPVSDKDVARDLLVKNIQEK
Sbjct: 121 PKLGLGIKHFQNRVLVSRVDPGSLAEKCLVLGDHLCDVDGIPVSDKDVARDLLVKNIQEK 180
Query: 181 GKVTFVVERPDSIDAKQWAKQALATNLMQPPSVQMNEDVKGIASQYRQALPGLKPPAKSA 240
GKVTFVVERPDSIDAKQWAKQALATNLMQPPSVQMNEDVKGIASQYRQALPGLKPPAKSA
Sbjct: 181 GKVTFVVERPDSIDAKQWAKQALATNLMQPPSVQMNEDVKGIASQYRQALPGLKPPAKSA 240
Query: 241 MSTGPSARRVSIVEQTQTHEIGHDHEGKALRKVK 274
MSTGPSARRVSIVEQTQTHEIGHDHEGKALRKVK
Sbjct: 241 MSTGPSARRVSIVEQTQTHEIGHDHEGKALRKVK 274
>pir||T15134 hypothetical protein T21G5.4 - Caenorhabditis elegans
gb|AAB52899.1| (AF000197) T21G5.4 gene product [Caenorhabditis elegans]
Length = 274
Score = 551 bits (1404), Expect = e-156
Identities = 271/274 (98%), Positives = 273/274 (98%)
Query: 1 MGSEKMIEICIDMEEGEPLGATPNDKLVITKIQAGTISEGKLRIGDQVKKVNGQNCKDCN 60
MGSEKMIEICIDMEEGEPLGATPNDKLVITKIQAGTISEGKLRIGDQVKKVNGQNCKDCN
Sbjct: 1 MGSEKMIEICIDMEEGEPLGATPNDKLVITKIQAGTISEGKLRIGDQVKKVNGQNCKDCN 60
Query: 61 DFFRALRFAAPCAKIMVNRDEKKAEELEARVHIPEDRAKIIQRREGYVYELATLVWVQNG 120
DFFRALRFAAPCAKI VNRDEKKAEELEARVHIPEDRAKIIQRREGYVYELATLVWVQNG
Sbjct: 61 DFFRALRFAAPCAKITVNRDEKKAEELEARVHIPEDRAKIIQRREGYVYELATLVWVQNG 120
Query: 121 PKLGLGIKHFQNRVLVSRVDPGSLAEKCLVLGDHLCDVDGIPVSDKDVARDLLVKNIQEK 180
PKLGLGIKHFQNRVLVSRVDPGSLAEKCLVLGDHLCDVDGIPVSDKDVARDLLVKNIQEK
Sbjct: 121 PKLGLGIKHFQNRVLVSRVDPGSLAEKCLVLGDHLCDVDGIPVSDKDVARDLLVKNIQEK 180
Query: 181 GKVTFVVERPDSIDAKQWAKQALATNLMQPPSVQMNEDVKGIASQYRQALPGLKPPAKSA 240
GKVTFVVERPDSIDAKQWAKQALATNLMQPPSVQMNEDVKGIASQYRQALPGLKPPAKSA
Sbjct: 181 GKVTFVVERPDSIDAKQWAKQALATNLMQPPSVQMNEDVKGIASQYRQALPGLKPPAKSA 240
Query: 241 MSTGPSARRVSIVEQTQTHEIGHDHEGKALRKVK 274
MSTGP+ARRVSI+EQTQTHEIGHDHEGKALRKVK
Sbjct: 241 MSTGPNARRVSIIEQTQTHEIGHDHEGKALRKVK 274
>pir||T22040 hypothetical protein F40F9.3 - Caenorhabditis elegans
emb|CAA94760.1| (Z70753) similar to PDZ domain (Also known as DHR or GLGF).
[Caenorhabditis elegans]
Length = 275
Score = 72.2 bits (174), Expect = 5e-12
Identities = 56/194 (28%), Positives = 91/194 (46%), Gaps = 14/194 (7%)
Query: 74 KIMVNRDEKKAEELEARVHIPEDRAKII-QRREGYVYELATLVWVQNGPKLGLGIKH-FQ 131
K+ + +++ + V IP +R K I +R+ GY Y + T+ + + G K GLGIK+ F
Sbjct: 34 KVSAEKAQEQEKSAMGSVPIPPEREKHIKERKTGYEYSIVTINY-KPGTKFGLGIKNVFN 92
Query: 132 NRVLVSRVDPGSLAEKCLVLGDHLCDVDGIPVSDKDVARDLLVKNIQEKGKVTFVVERPD 191
+ V +V+ SL + D + DVDG PV D + LLVK ++EK T ++ER
Sbjct: 93 KSIYVIKVEENSLVSGIFNVADRIIDVDGEPVYDNAKCKGLLVKALKEKKVCTLLIERGI 152
Query: 192 S----IDAKQWAKQALATNLMQPPSV-------QMNEDVKGIASQYRQALPGLKPPAKSA 240
+ DA + ++M PP V + + ++ S A P P A +
Sbjct: 153 TESALKDATNEMNEEHTQSVMAPPDVKSIMRKLEAHNFLRKSPSCESAAAPNPVPSAMTK 212
Query: 241 MSTGPSARRVSIVE 254
G R+ I+E
Sbjct: 213 NKEGKGERKACILE 226
>pir||T32784 hypothetical protein C50D2.3 - Caenorhabditis elegans
gb|AAB94951.1| (AF040642) C50D2.3 gene product [Caenorhabditis elegans]
Length = 152
Score = 51.2 bits (120), Expect = 1e-05
Identities = 33/100 (33%), Positives = 54/100 (54%), Gaps = 3/100 (3%)
Query: 113 TLVWVQNGPKLGLGIKHFQNRVLVSRVDPGSLAEKCLVLGDHLCDVDGIPVSDKDVARDL 172
TL+ Q G K GLGI R+LV +V+ SL L GD + +++ V +K +
Sbjct: 48 TLMMTQ-GKKFGLGIVSVHQRILVCKVENESLVAGVLKYGDQIIEINKKNVLNKIDCKKR 106
Query: 173 LVKNIQEKGKVTFVVERPDSIDAKQWAKQALATNLMQPPS 212
L++ +++KG+V ++ RP + DA +Q + L Q PS
Sbjct: 107 LMQCLKDKGQVDIIIVRPKTADAVTMIEQEI--QLSQQPS 144
>pir||T00346 hypothetical protein KIAA0583 - human (fragment)
dbj|BAA25509.1| (AB011155) KIAA0583 protein [Homo sapiens]
Length = 1281
Score = 47.6 bits (111), Expect = 1e-04
Identities = 45/199 (22%), Positives = 95/199 (47%), Gaps = 30/199 (15%)
Query: 13 MEEGEPLGATPND-KLVITKIQAGTISEGKLRIGDQVKKVNGQNCKDCNDFFRALRFAAP 71
M EG P D + +TK+ G+I++G+LR+ D + ++N +
Sbjct: 1 MAEGVNEPCFPGDCGIFVTKVDKGSIADGRLRVNDWLLRINDVD---------------- 44
Query: 72 CAKIMVNRDEKKAEELEARVHIPEDRAKIIQRREGYVYELATLVWVQNGPKLGLGIKHFQ 131
++N+D+K+A ++A ++ +++RR+ ++ T + + + GI +
Sbjct: 45 ----LINKDKKQA--IKALLNGEGAINMVVRRRKSLGGKVVTPLHINLSGQKDSGIS-LE 97
Query: 132 NRVLVSRVDPGSLAEK--CLVLGDHLCDVDGIPVSDKDVAR-DLLVKNIQEKGKVTFVVE 188
N V + V PGS A K L +GD + ++GI + +K + + L+++ Q+ ++ +
Sbjct: 98 NGVYAAAVLPGSPAAKEGSLAVGDRIVAINGIALDNKSLNECESLLRSCQDSLTLSLLKV 157
Query: 189 RPDSIDAKQWAKQALATNL 207
P S W+ Q + N+
Sbjct: 158 FPQS---SSWSGQNIFENI 173
>pir||T25637 hypothetical protein C46H11.6 - Caenorhabditis elegans
gb|AAB42360.1| (U88314) C46H11.6 gene product [Caenorhabditis elegans]
Length = 342
Score = 46.1 bits (107), Expect = 4e-04
Identities = 34/127 (26%), Positives = 65/127 (50%), Gaps = 10/127 (7%)
Query: 123 LGLGIKHFQNRVLVSRVDPGSLAEKCLVLGDHLCDVDGIPVSDKDVARDLL---VKNIQE 179
+G+ + + Q R +V+R++P SLA GD + D++ + + + D + + I +
Sbjct: 145 VGVTVTYIQKRGIVTRIEPSSLASIFFGRGDLIMDINEESILNAEKNDDFIREKLSGILK 204
Query: 180 KGKVTFVVERPDSIDAKQWAKQALATNLMQPPSVQMNEDV----KGIASQYRQALPGLKP 235
KVTF+VERP S+++ + L ++ P V+M D + ++ + L L+P
Sbjct: 205 GEKVTFLVERPVSVESANTLRH-LIESVAPDPDVKMANDAICIGRNASNMHYNVLKKLQP 263
Query: 236 PAKSAMS 242
KS +S
Sbjct: 264 --KSILS 268
>pir||T16202 hypothetical protein F28E10.4 - Caenorhabditis elegans
gb|AAA81502.1| (U40801) F28E10.4 gene product [Caenorhabditis elegans]
Length = 272
Score = 45.7 bits (106), Expect = 5e-04
Identities = 26/100 (26%), Positives = 50/100 (50%), Gaps = 3/100 (3%)
Query: 123 LGLGIKHFQNRVLVSRVDPGSLAEKCLVLGDHLCDVDGIPVSDKDVARDLL---VKNIQE 179
+GL + + R V++VDP ++A GD + D+ G P+ D + + + +
Sbjct: 82 VGLKLGIIKRRAFVTKVDPNTIASSFFGCGDSIMDMSGAPIPMTDNPDNFIREHLSRLST 141
Query: 180 KGKVTFVVERPDSIDAKQWAKQALATNLMQPPSVQMNEDV 219
KV+F+VERP S+ + ++ + + V+M +DV
Sbjct: 142 GAKVSFLVERPISVAMAKQYQKFIESITYDDSEVEMAQDV 181
>emb|CAB39895.1| (AJ133439) GRIP1 protein [Homo sapiens]
Length = 849
Score = 42.2 bits (97), Expect = 0.006
Identities = 43/235 (18%), Positives = 99/235 (41%), Gaps = 26/235 (11%)
Query: 18 PLGATPNDKLVITKIQAGTISE--GKLRIGDQVKKVNGQNCKDCN--DFFRALRFAAPCA 73
P P D LVI+ I+ G+++ G L +GD++ ++ +C+ D + L+
Sbjct: 327 PSSRKPGDPLVISDIKKGSVAHRTGTLELGDKLLAIDNIRLDNCSMEDAVQILQQCEDLV 386
Query: 74 KIMVNRDEKKAEELEARVHIPEDRAKIIQRREGYVYELATLVWVQNGPKLGLGIKHFQ-- 131
K+ + +DE ++E E+ I + T+ + G LG+ I +
Sbjct: 387 KLKIRKDEDNSDEQESSGAI-----------------IYTVELKRYGGPLGITISGTEEP 429
Query: 132 -NRVLVSRVDPGSLAEK--CLVLGDHLCDVDGIPVSDKDVARDLLVKNIQEKGKVTFVVE 188
+ +++S + G LAE+ + +GD + ++ + K ++ + + + + + +
Sbjct: 430 FDPIIISSLTKGGLAERTGAIHIGDRILAINSSSLKGKPLSEAIHLLQMAGETVTLKIKK 489
Query: 189 RPDSIDAKQWAKQALATNLMQPPSVQMNEDVKGIASQYRQALPGLKPPAKSAMST 243
+ D+ A K ++++L V+ + + P P SA+ +
Sbjct: 490 QTDAQSASSPKKFPISSHLSDLGDVEEDSSPAQKPGKLSDMYPSTVPSVDSAVDS 544
>pir||T16122 hypothetical protein F20D6.1 - Caenorhabditis elegans
gb|AAB37048.1| (U50301) F20D6.1 gene product [Caenorhabditis elegans]
Length = 269
Score = 42.2 bits (97), Expect = 0.006
Identities = 33/131 (25%), Positives = 54/131 (41%), Gaps = 17/131 (12%)
Query: 75 IMVNRDEKKAEELEARVHIPEDRAKIIQRREGYVYELATLVWVQNGPKLGLGIKHFQN-- 132
I V R ++++ A + K ++ GY+Y L++ KLG+ +K +QN
Sbjct: 43 ISVERLDERSIPANAAIRKKGSSDKSVKPNVGYMYYKLVLIYFPRS-KLGINVKSYQNVD 101
Query: 133 ------------RVLVSRVDP--GSLAEKCLVLGDHLCDVDGIPVSDKDVARDLLVKNIQ 178
V V D GS + L LGD + +D + D ++ L
Sbjct: 102 SATFFFLKLDAEEVYVESTDNTWGSTTRRFLYLGDAILRIDDTEILDLPTTQNALRTGFS 161
Query: 179 EKGKVTFVVER 189
+ G VT +VER
Sbjct: 162 KNGVVTIIVER 172
>pir||T29298 hypothetical protein C50F7.3 - Caenorhabditis elegans
gb|AAA83306.1| (U41557) C50F7.3 gene product [Caenorhabditis elegans]
Length = 313
Score = 41.8 bits (96), Expect = 0.008
Identities = 41/183 (22%), Positives = 78/183 (42%), Gaps = 21/183 (11%)
Query: 40 GKLRIGDQVKKVNGQNCKDCN---DFFRALRFAAPCAKIMVNRDEKKAEELEARVHIPED 96
G +++GD + +NG F + + PCAK+ + + + HIP
Sbjct: 59 GLIKVGDILVYLNGYQIGGQGVLARFIQTIINGQPCAKMTY-------KVIRFKRHIPRP 111
Query: 97 RA-KIIQRREGYVYELATLVWVQNGPKLGLGIKHFQNRVLVSRVDPGSLAEKCLVLGDHL 155
+Q+ E Y + L ++ LGL IK + +++V + SL + +G+ +
Sbjct: 112 SGFPPLQKHESYSTDTLVLYNLKKVFHLGLDIKEIEGKIVVCDLVENSLGDMTFSVGETI 171
Query: 156 CDVDG-IPVS----DKDVARDLLVKNIQEKGKVTFVVERPDSIDAKQWAKQALATNLMQP 210
DVDG P+S ++ V + LL++N VE P + K + + + +
Sbjct: 172 LDVDGEKPLSCTGFNERVRKSLLIRNY-----CLVTVEIPSTDPLKNLLRNQITKTVKEA 226
Query: 211 PSV 213
P +
Sbjct: 227 PRI 229
Database: nr
Posted date: Jul 9, 2000 11:06 PM
Number of letters in database: 162,524,432
Number of sequences in database: 517,904
Lambda K H
0.316 0.135 0.388
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 86150064
Number of Sequences: 517904
Number of extensions: 3402781
Number of successful extensions: 10088
Number of sequences better than 10.0: 67
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 58
Number of HSP's that attempted gapping in prelim test: 10017
Number of HSP's gapped (non-prelim): 143
length of query: 274
length of database: 162,524,432
effective HSP length: 56
effective length of query: 218
effective length of database: 133,521,808
effective search space: 29107754144
effective search space used: 29107754144
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.6 bits)
S2: 70 (31.7 bits)
