東京大学 生物生産工学研究センター 細胞機能工学研究室

〒113-8657 東京都文京区弥生1-1-1
東京大学生物生産工学研究センター
TEL:03-5841-3074

研究業績

2020年

2019年

  • Murai K, Lauterbach L, Teramoto K, Quan Z, Barra L, Yamamoto T, Nonaka K, Shiomi K, Nishiyama M, Kuzuyama T, Dickschat JS.
    An Unusual Skeletal Rearrangement in the Biosynthesis of the Sesquiterpene Trichobrasilenol from Trichoderma.
    Angew Chem Int Ed Engl. 2019 Oct 14;58(42):15046-15050. doi: 10.1002/anie.201907964.
  • Kobayashi M, Tomita T, Shin-Ya K, Nishiyama M, Kuzuyama T.
    An Unprecedented Cyclization Mechanism in the Biosynthesis of Carbazole Alkaloids in Streptomyces.
    Angew Chem Int Ed Engl. 2019 Sep 16;58(38):13349-13353. doi: 10.1002/anie.201906864.
  • Suzuki T, Akiyama N, Yoshida A, Tomita T, Lassak K, Haurat MF, Okada T, Takahashi K, Albers SV, Kuzuyama T, Nishiyama M.
    Biochemical characterization of archaeal homocitrate synthase from Sulfolobus acidocaldarius.
    FEBS Lett. 2019 Jul 22. doi: 10.1002/1873-3468.13550.
  • Kudo K, Koiwai H, Kagaya N, Nishiyama M, Kuzuyama T, Shin-Ya K, Ikeda H.
    Comprehensive Derivatization of Thioviridamides by Heterologous Expression.
    ACS Chem Biol. 2019 Jun 11. doi: 10.1021/acschembio.9b00330.
  • Romo AJ, Shiraishi T, Ikeuchi H, Lin GM, Geng Y, Lee YH, Liem PH, Ma T, Ogasawara Y, Shin-Ya K, Nishiyama M, Kuzuyama T, Liu HW.
    The Amipurimycin and Miharamycin Biosynthetic Gene Clusters: Unraveling the Origins of 2-Aminopurinyl Peptidyl Nucleoside Antibiotics.
    J Am Chem Soc. 2019 May 31. doi: 10.1021/jacs.9b03021.
  • Tomita T, Matsushita H, Yoshida A, Kosono S, Yoshida M, Kuzuyama T, Nishiyama M.
    Glutamate dehydrogenase from Thermus thermophilus is activated by AMP and leucine as a complex with catalytically inactive adenine phosphoribosyltransferase homolog.
    J Bacteriol. 2019 Apr 29. pii: JB.00710-18. doi: 10.1128/JB.00710-18.
  • Yoshida A, Yoshida M, Kuzuyama T, Nishiyama M, Kosono S.
    Protein acetylation on 2-isopropylmalate synthase from Thermus thermophilus HB27.
    Extremophiles. 2019 Jul;23(4):377-388. doi: 10.1007/s00792-019-01090-y.
  • Yashiro T, Sakata F, Sekimoto T, Shirai T, Hasebe F, Matsuda K, Kurosawa S, Suzuki S, Nagata K, Kasakura K, Nishiyama M, Nishiyama C.
    Immunosuppressive Effect of a Non-Proteinogenic Amino Acid From Streptomyces Through Inhibiting Allogeneic T Cell Proliferation.
    Biosci Biotechnol Biochem. 2019 Jun;83(6):1111-1116. doi: 10.1080/09168451.2019.1591262.
  • Shiraishi T, Nishiyama M, Kuzuyama T.
    Biosynthesis of the uridine-derived nucleoside antibiotic A-94964: identification and characterization of the biosynthetic gene cluster provide insight into the biosynthetic pathway.
    Org Biomol Chem. 2019 Jan 16;17(3):461-466. doi: 10.1039/c8ob02765j.
  • Chiba Y, Yoshida A, Shimamura S, Kameya M, Tomita T, Nishiyama M, Takai K.
    Discovery and analysis of a novel type of the serine biosynthetic enzyme phosphoserine phosphatase in Thermus thermophilus.
    FEBS J. 2019 Feb;286(4):726-736. doi: 10.1111/febs.14703.
  • Suzuki S, Kondo N, Yoshida M, Nishiyama M, Kosono S.
    Dynamic changes in the acetylation and succinylation of the elongation factor Tu from Bacillus subtilis are dependent on the growth phase and nutrient conditions.
    Microbiology. 2019 Jan 1;165(1):65-77. doi: 10.1099/mic.0.000737

2018年

  • Umehara T, Kosono S, Söll D, Tamura K.
    Lysine Acetylation Regulates Alanyl-tRNA Synthetase Activity in Escherichia coli.
    Genes (Basel). 2018 Sep 28;9(10):473. doi: 10.3390/genes9100473.
  • Thong WL, Shin-ya K, Nishiyama M, Kuzuyama T.
    Discovery of an antibacterial isoindolinone-containing tetracyclic polyketide by cryptic gene activation and characterization of its biosynthetic gene cluster.
    ACS Chem Biol. 2018 Sep 21;13(9):2615-2622. doi: 10.1021/acschembio.8b00553.
  • Matsuda K, Tomita T, Shin-ya K, Wakimoto T, Kuzuyama T, Nishiyama M.
    Discovery of unprecedented hydrazine-forming machinery in bacteria.
    J Am Chem Soc. 2018 Jul 25;140(29):9083-9086. doi: 10.1021/jacs.8b05354.
  • Yoshida A, Kosono S, Nishiyama M.
    Characterization of two 2-isopropylmalate synthase homologs from Thermus thermophilus HB27.
    Biochem Biophys Res Commun. 2018 Jun 22;501(2):465-470. doi: 10.1016/j.bbrc.2018.05.013.
  • Vasileva D, Suzuki-Minakuchi C, Kosono S, Yoshida M, Okada K, Nojiri H.
    Proteome and acylome analyses of the functional interaction network between the carbazole-degradative plasmid pCAR1 and host Pseudomonas putida KT2440.
    Environ Microbiol Rep. 2018 Jun;10(3):299-309. doi: 10.1111/1758-2229.12639.
  • Zheng RC, Hachisuka SI, Tomita H, Imanaka T, Zheng YG, Nishiyama M, Atomi H.
    An ornithine ω-aminotransferase required for growth in the absence of exogenous proline in the archaeon Thermococcus kodakarensis.
    J Biol Chem. 2018 Mar 9;293(10):3625-3636. doi: 10.1074/jbc.RA117.001222.
  • Hossain TJ, Manabe S, Ito Y, Iida T, Kosono S, Ueda K, Hosomi A, Inoue D, Suzuki T.
    Enrichment and characterization of a bacterial mixture capable of utilizing C-mannosyl tryptophan as a carbon source.
    Glycoconj J. 2018 Apr;35(2):165-176. doi: 10.1007/s10719-017-9807-2.

2017年

2016年

2015年

  • Sato H, Teramoto K, Masumoto Y, Tezuka N, Sakai K, Ueda S, Totsuka Y, Shinada T, Nishiyama M, Wang C, Kuzuyama T, Uchiyama M.
    "Cation-Stitching Cascade": exquisite control of terpene cyclization in cyclooctatin biosynthesis.
    Sci Rep. 2015 Dec 18;5:18471. doi: 10.1038/srep18471.
  • Kosono S, Tamura M, Suzuki S, Kawamura Y, Yoshida A, Nishiyama M, Yoshida M.
    Changes in the Acetylome and Succinylome of Bacillus subtilis in Response to Carbon Source.
    PLoS One. 2015 Jun 22;10(6):e0131169. doi: 10.1371/journal.pone.0131169. eCollection 2015.
  • Meguro A, Motoyoshi Y, Teramoto K, Ueda S, Totsuka Y, Ando Y, Tomita T, Kim SY, Kimura T, Igarashi M, Sawa R, Shinada T, Nishiyama M, Kuzuyama T.
    An unusual terpene cyclization mechanism involving a carbon-carbon bond rearrangement.
    Angew Chem Int Ed Engl. 2015 Mar 27;54(14):4353-6. doi: 10.1002/anie.201411923.
  • Hashimoto T, Hashimoto J, Teruya K, Hirano T, Shin-ya K, Ikeda H, Liu HW, Nishiyama M, Kuzuyama T.
    Biosynthesis of versipelostatin: identification of an enzyme-catalyzed [4+2]-cycloaddition required for macrocyclization of spirotetronate-containing polyketides.
    J Am Chem Soc. 2015 Jan 21;137(2):572-5. doi: 10.1021/ja510711x.
  • Yoshida A, Tomita T, Fujimura T, Nishiyama C, Kuzuyama T, Nishiyama M.
    Structural insight into amino group-carrier protein-mediated lysine biosynthesis: crystal structure of the LysZ・LysW complex from Thermus thermophilus.
    J Biol Chem. 2015 Jan 2;290(1):435-47. doi: 10.1074/jbc.M114.595983.
  • Lin J, Nishiyama M, Kuzuyama T.
    Identification of the biosynthetic gene cluster for the herbicide phosphonothrixin in Saccharothrix sp. ST-888.
    J Antibiot (Tokyo). 2015 May;68(5):357-9. doi: 10.1038/ja.2014.149.

2014年

  • Tomita T, Ozaki T, Matsuda K, Nishiyama M, Kuzuyama T.
    Crystallization and preliminary X-ray diffraction analysis of cyclolavandulyl diphosphate synthase, a new member of the cis-isoprenyl diphosphate synthase superfamily.
    Acta Crystallogr F Struct Biol Commun. 2014 Oct;70(Pt 10):1410-3. doi: 10.1107/S2053230X14018883.
  • Iwanaga N, Ide K, Nagashima T, Tomita T, Agari Y, Shinkai A, Kuramitsu S, Okada-Hatakeyema M, Kuzuyama T, Nishiyama M.
    Genome-wide comprehensive analysis of transcriptional regulation by ArgR in Thermus thermophilus.
    Extremophiles. 2014 Nov;18(6):995-1008. doi: 10.1007/s00792-014-0669-2.
  • Park JS, Yabe S, Shin-Ya K, Nishiyama M, Kuzuyama T.
    New 2-(1'H-indole-3'-carbonyl)-thiazoles derived from the thermophilic bacterium Thermosporothrix hazakensis SK20-1T.
    J Antibiot (Tokyo). 2015 Jan;68(1):60-2. doi: 10.1038/ja.2014.93.
  • Ozaki T, Zhao P, Shinada T, Nishiyama M, Kuzuyama T.
    Cyclolavandulyl skeleton biosynthesis via both condensation and cyclization catalyzed by an unprecedented member of the cis-isoprenyl diphosphate synthase superfamily.
    J Am Chem Soc. 2014 Apr 2;136(13):4837-40. doi: 10.1021/ja500270m.
  • Park JS, Kagaya N, Hashimoto J, Izumikawa M, Yabe S, Shin-Ya K, Nishiyama M, Kuzuyama T.
    Identification and Biosynthesis of New Acyloins from the Thermophilic Bacterium Thermosporothrix hazakensis SK20-1T.
    Chembiochem. 2014 Mar 3;15(4):527-32. doi: 10.1002/cbic.201300690.
  • Yasutake Y, Kitagawa W, Hata M, Nishioka T, Ozaki T, Nishiyama M, Kuzuyama T, Tamura T.
    Structure of the quinoline N-hydroxylating cytochrome P450 RauA, an essential enzyme that confers antibiotic activity on aurachin alkaloids.
    FEBS Lett. 2014 Jan 3;588(1):105-10. doi: 10.1016/j.febslet.2013.11.016.
  • Nakao R, Takashiba S, Kosono S, Yoshida M, Watanabe H, Ohnishi M, Senpuku H.
    Effect of Porphyromonas gingivalis outer membrane vesicles on gingipain-mediated detachment of cultured oral epithelial cells and immune responses.
    Microbes Infect. 2014 Jan;16(1):6-16. doi: 10.1016/j.micinf.2013.10.005.
  • Schendzielorz G, Dippong M, Grünberger A, Kohlheyer D, Yoshida A, Binder S, Nishiyama C, Nishiyama M, Bott M, Eggeling L.
    Taking control over control: use of product sensing in single cells to remove flux control at key enzymes in biosynthesis pathways.
    ACS Synth Biol. 2014 Jan 17;3(1):21-9. doi: 10.1021/sb400059y.

2013年

  • Kanemaru Y, Hasebe F, Tomita T, Kuzuyama T, Nishiyama M.
    Two ATP-binding cassette transporters involved in (S)-2-aminoethyl-cysteine uptake in Thermus thermophilus.
    J Bacteriol. 2013 Sep;195(17):3845-53. doi: 10.1128/JB.00202-13.
  • Kitagawa W, Ozaki T, Nishioka T, Yasutake Y, Hata M, Nishiyama M, Kuzuyama T, Tamura T.
    Cloning and heterologous expression of the aurachin RE biosynthesis gene cluster afford a new cytochrome P450 for quinoline N-hydroxylation.
    Chembiochem. 2013 Jun 17;14(9):1085-93. doi: 10.1002/cbic.201300167.
  • Ouchi T, Tomita T, Horie A, Yoshida A, Takahashi K, Nishida H, Lassak K, Taka H, Mineki R, Fujimura T, Kosono S, Nishiyama C, Masui R, Kuramitsu S, Albers SV, Kuzuyama T, Nishiyama M.
    Lysine and arginine biosyntheses mediated by a common carrier protein in Sulfolobus.
    Nat Chem Biol. 2013 Apr;9(4):277-83. doi: 10.1038/nchembio.1200.
  • Ozaki T, Nishiyama M, Kuzuyama T.
    Novel Tryptophan Metabolism by a Potential Gene Cluster That Is Widely Distributed among Actinomycetes.
    J Biol Chem. 2013 Apr 5;288(14):9946-56. doi: 10.1074/jbc.M112.436451.
  • Meguro A, Tomita T, Nishiyama M, Kuzuyama T.
    Identification and characterization of bacterial diterpene cyclases that synthesize the cembrane skeleton.
    Chembiochem. 2013 Feb 11;14(3):316-21. doi: 10.1002/cbic.201200651.
  • Hara M, Yokoyama H, Fukuyama K, Kitamura N, Shimokawa N, Maeda K, Kanada S, Ito T, Usui Y, Ogawa H, Okumura K, Nishiyama M, Nishiyama C.
    Transcriptional regulation of the mouse CD11c promoter by AP-1 complex with JunD and Fra2 in dendritic cells.
    Mol Immunol. 2013 Mar;53(3):295-301. doi: 10.1016/j.molimm.2012.08.004.

2012年

  • Isogai S, Nishiyama M, Kuzuyama T.
    Identification of 8-amino-2,5,7-trihydroxynaphthalene-1,4-dione, a novel intermediate in the biosynthesis of Streptomyces meroterpenoids.
    Bioorg Med Chem Lett. 2012 Sep 15;22(18):5823-6. doi: 10.1016/j.bmcl.2012.07.084.
  • Nishida H, Nishiyama M.
    Evolution of lysine biosynthesis in the phylum deinococcus-thermus.
    Int J Evol Biol. 2012;2012:745931. doi: 10.1155/2012/745931.
  • Kitamura N, Yokoyama H, Yashiro T, Nakano N, Nishiyama M, Kanada S, Fukai T, Hara M, Ikeda S, Ogawa H, Okumura K, Nishiyama C.
    Role of PU.1 in MHC class II expression through transcriptional regulation of class II transactivator pI in dendritic cells.
    J Allergy Clin Immunol. 2012 Mar;129(3):814-824.e6. doi: 10.1016/j.jaci.2011.10.019.

2011年

  • Tomita T, Kuzuyama T, Nishiyama M.
    Structural basis for leucine-induced allosteric activation of glutamate dehydrogenase.
    J Biol Chem. 2011 Oct 28;286(43):37406-13. doi: 10.1074/jbc.M111.260265.
  • Nishida C, Tomita T, Nishiyama M, Suzuki R, Hara M, Itoh Y, Ogawa H, Okumura K, Nishiyama C.
    B-transferase with a Pro234Ser substitution acquires AB-transferase activity.
    Biosci Biotechnol Biochem. 2011;75(8):1570-5. doi: 10.1271/bbb.110276.
  • Dairi T, Kuzuyama T, Nishiyama M, Fujii I.
    Convergent strategies in biosynthesis.
    Nat Prod Rep. 2011 Jun;28(6):1054-86. doi: 10.1039/c0np00047g.
  • Shindo K, Tachibana A, Tanaka A, Toba S, Yuki E, Ozaki T, Kumano T, Nishiyama M, Misawa N, Kuzuyama T.
    Production of novel antioxidative prenyl naphthalen-ols by combinational bioconversion with dioxygenase PhnA1A2A3A4 and prenyltransferase NphB or SCO7190.
    Biosci Biotechnol Biochem. 2011;75(3):505-10. doi: 10.1271/bbb.100731.
  • Ogawa A, Furukawa S, Fujita S, Mitobe J, Kawarai T, Narisawa N, Sekizuka T, Kuroda M, Ochiai K, Ogihara H, Kosono S, Yoneda S, Watanabe H, Morinaga Y, Uematsu H, Senpuku H.
    Inhibition of Streptococcus mutans biofilm formation by Streptococcus salivarius FruA. Appl Environ Microbiol. 2011 Mar;77(5):1572-80. doi: 10.1128/AEM.02066-10.

2010年

2009年

  • Kim SY, Zhao P, Igarashi M, Sawa R, Tomita T, Nishiyama M, Kuzuyama T.
    Cloning and heterologous expression of the cyclooctatin biosynthetic gene cluster afford a diterpene cyclase and two P450 hydroxylases.
    Chem Biol. 2009 Jul 31;16(7):736-43. doi: 10.1016/j.chembiol.2009.06.007.
  • Ouchi T, Tomita T, Miyagawa T, Kuzuyama T, Nishiyama M.
    Dual roles of a conserved pair, Arg23 and Ser20, in recognition of multiple substrates in alpha-aminoadipate aminotransferase from Thermus thermophilus.
    Biochem Biophys Res Commun. 2009 Oct 9;388(1):21-7. doi: 10.1016/j.bbrc.2009.07.096.
  • Horie A, Tomita T, Saiki A, Kono H, Taka H, Mineki R, Fujimura T, Nishiyama C, Kuzuyama T, Nishiyama M.
    Discovery of proteinaceous N-modification in lysine biosynthesis of Thermus thermophilus.
    Nat Chem Biol. 2009 Sep;5(9):673-9. doi: 10.1038/nchembio.198.
  • Ozaki T, Mishima S, Nishiyama M, Kuzuyama T.
    NovQ is a prenyltransferase capable of catalyzing the addition of a dimethylallyl group to both phenylpropanoids and flavonoids.
    J Antibiot (Tokyo). 2009 Jul;62(7):385-92. doi: 10.1038/ja.2009.48.
  • Ito T, Nishiyama C, Nakano N, Nishiyama M, Usui Y, Takeda K, Kanada S, Fukuyama K, Akiba H, Tokura T, Hara M, Tsuboi R, Ogawa H, Okumura K.
    Roles of PU.1 in monocyte- and mast cell-specific gene regulation: PU.1 transactivates CIITA pIV in cooperation with IFN-gamma.
    Int Immunol. 2009 Jul;21(7):803-16. doi: 10.1093/intimm/dxp048.
  • Yoshida A, Tomita T, Kono H, Fushinobu S, Kuzuyama T, Nishiyama M.
    Crystal structures of the regulatory subunit of Thr-sensitive aspartate kinase from Thermus thermophilus.
    FEBS J. 2009 Jun;276(11):3124-36. doi: 10.1111/j.1742-4658.2009.07030.x.
  • Kajiyama Y, Otagiri M, Sekiguchi J, Kudo T, Kosono S.
    The MrpA, MrpB and MrpD subunits of the Mrp antiporter complex in Bacillus subtilis contain membrane-embedded and essential acidic residues.
    Microbiology. 2009 Jul;155(Pt 7):2137-2147. doi: 10.1099/mic.0.025205-0.
  • Zhao P, Ueda JY, Kozone I, Chijiwa S, Takagi M, Kudo F, Nishiyama M, Shin-ya K, Kuzuyama T.
    New glycosylated derivatives of versipelostatin, the GRP78/Bip molecular chaperone down-regulator, from Streptomyces versipellis 4083-SVS6.
    Org Biomol Chem. 2009 Apr 7;7(7):1454-60. doi: 10.1039/b817312e.
  • Tomita T, Miyagawa T, Miyazaki T, Fushinobu S, Kuzuyama T, Nishiyama M.
    Mechanism for multiple-substrates recognition of alpha-aminoadipate aminotransferase from Thermus thermophilus.
    Proteins. 2009 May 1;75(2):348-59. doi: 10.1002/prot.22245.

2008年

  • Kumano T, Richard SB, Noel JP, Nishiyama M, Kuzuyama T.
    Chemoenzymatic syntheses of prenylated aromatic small molecules using Streptomyces prenyltransferases with relaxed substrate specificities.
    Bioorg Med Chem. 2008 Sep 1;16(17):8117-26. doi: 10.1016/j.bmc.2008.07.052.
  • Opr86 is essential for viability and is a potential candidate for a protective antigen against biofilm formation by Pseudomonas aeruginosa.
    J Bacteriol. 2008 Jun;190(11):3969-78. doi: 10.1128/JB.02004-07.
  • Nakao R, Tashiro Y, Nomura N, Kosono S, Ochiai K, Yonezawa H, Watanabe H, Senpuku H.
    Glycosylation of the OMP85 homolog of Porphyromonas gingivalis and its involvement in biofilm formation.
    Biochem Biophys Res Commun. 2008 Jan 25;365(4):784-9. doi: 10.1016/j.bbrc.2007.11.035.
  • Takagi A, Nishiyama C, Maeda K, Tokura T, Kawada H, Kanada S, Niwa Y, Nakano N, Mayuzumi N, Nishiyama M, Ikeda S, Okumura K, Ogawa H.
    Role of Sp1 in transcription of human ATP2A2 gene in keratinocytes.
    J Invest Dermatol. 2008 Jan;128(1):96-103. doi: 10.1038/sj.jid.5700937.

2007年

  • Sobajima H, Tani T, Chujo T, Okada K, Suzuki K, Mori S, Minami E, Nishiyama M, Nojiri H, Yamane H.
    Identification of a jasmonic acid-responsive region in the promoter of the rice 12-oxophytodienoic acid reductase 1 gene OsOPR1.
    Biosci Biotechnol Biochem. 2007 Dec;71(12):3110-5. doi: 10.1271/bbb.70532.
  • Kajiyama Y, Otagiri M, Sekiguchi J, Kosono S, Kudo T.
    Complex formation by the mrpABCDEFG gene products, which constitute a principal Na+/H+ antiporter in Bacillus subtilis.
    J Bacteriol. 2007 Oct;189(20):7511-4. doi: 10.1128/JB.00968-07.
  • Yoshida A, Tomita T, Kurihara T, Fushinobu S, Kuzuyama T, Nishiyama M.
    Structural Insight into Concerted Inhibition of alpha2beta2-Type Aspartate Kinase from Corynebacterium glutamicum.
    J Mol Biol. 2007 Apr 27;368(2):521-36. doi: 10.1016/j.jmb.2007.02.017.
  • Nakano N, Nishiyama C, Kanada S, Niwa Y, Shimokawa N, Ushio H, Nishiyama M, Okumura K, Ogawa H.
    Involvement of mast cells in IL-12/23 p40 production is essential for survival from polymicrobial infections.
    Blood. 2007 Jun 1;109(11):4846-55. doi: 10.1182/blood-2006-09-045641.
  • Yoshida A, Tomita T, Kuzuyama T, Nishiyama M.
    Purification, crystallization and preliminary X-ray analysis of regulatory subunit of aspartate kinase from Thermus thermophilus.
    Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Feb 1;63(Pt 2):96-8. doi: 10.1107/S1744309106055837.
  • Natori Y, Nanamiya H, Akanuma G, Kosono S, Kudo T, Ochi K, Kawamura F.
    A fail-safe system for the ribosome under zinc-limiting conditions in Bacillus subtilis.
    Mol Microbiol. 2007 Jan;63(1):294-307. doi: 10.1111/j.1365-2958.2006.05513.x.

2006年

  • Nanamiya H, Kawamura F, Kosono S.
    Proteomic study of the Bacillus subtilis ribosome: Finding of zinc-dependent replacement for ribosomal protein L31 paralogues.
    J Gen Appl Microbiol. 2006 Oct;52(5):249-58. doi: 10.2323/jgam.52.249.
  • Fujiwara K, Tsubouchi T, Kuzuyama T, Nishiyama M.
    Involvement of arginine repressor in lysine biosynthesis of Thermus thermophilus.
    Microbiology. 2006 Dec;152(Pt 12):3585-3594. doi: 10.1099/mic.0.29222-0.
  • Tomita T, Kuzuyama T, Nishiyama M.
    Alteration of coenzyme specificity of lactate dehydrogenase from Thermus thermophilus by introducing the loop region of NADP(H)-dependent malate dehydrogenase.
    Biosci Biotechnol Biochem. 2006 Sep;70(9):2230-5. doi: 10.1271/bbb.60170.
  • Tomita T, Fushinobu S, Kuzuyama T, Nishiyama M.
    Structural basis for the alteration of coenzyme specificity in a malate dehydrogenase from Thermus flavus.
    Biochem Biophys Res Commun. 2006 Aug 25;347(2):502-8. doi: 10.1016/j.bbrc.2006.06.131.
  • Kosono S, Kajiyama Y, Kawasaki S, Yoshinaka T, Haga K, Kudo T.
    Functional involvement of membrane-embedded and conserved acidic residues in the ShaA subunit of the multigene-encoded Na+/H+ antiporter in Bacillus subtilis.
    Biochim Biophys Acta. 2006 May;1758(5):627-35. doi: 10.1016/j.bbamem.2006.04.012.
  • Jia Y, Tomita T, Yamauchi K, Nishiyama M, Palmer DR.
    Kinetics and product analysis of the reaction catalyzed by recombinant homoaconitase from Thermus thermophilus.
    Biochem J. 2006 Jun 15;396(3):479-85. doi: 10.1042/BJ20051711.
  • Maeda K, Nishiyama C, Tokura T, Nakano H, Kanada S, Nishiyama M, Okumura K, Ogawa H.
    FOG-1 represses GATA-1-dependent FceRI beta-chain transcription: transcriptional mechanism of mast cell-specific gene expression in mice.
    Blood. 2006 Jul 1;108(1):262-9. doi: 10.1182/blood-2005-07-2878.

2005年

  • Miyazaki J, Asada K, Fushinobu S, Kuzuyama T, Nishiyama M.
    Crystal structure of tetrameric homoisocitrate dehydrogenase from an extreme thermophile, Thermus thermophilus: involvement of hydrophobic dimer-dimer interaction in extremely high thermotolerance.
    J Bacteriol. 2005 Oct;187(19):6779-88. doi: 10.1128/JB.187.19.6779-6788.2005.
  • Kosono S, Haga K, Tomizawa R, Kajiyama Y, Hatano K, Takeda S, Wakai Y, Hino M, Kudo T.
    Characterization of a multigene-encoded sodium/hydrogen antiporter (sha) from Pseudomonas aeruginosa: its involvement in pathogenesis.
    J Bacteriol. 2005 Aug;187(15):5242-8. doi: 10.1128/JB.187.15.5242-5248.2005.
  • Tomita T, Fushinobu S, Kuzuyama T, Nishiyama M.
    Crystal structure of NAD-dependent malate dehydrogenase complexed with NADP(H).
    Biochem Biophys Res Commun. 2005 Aug 26;334(2):613-8. doi: 10.1016/j.bbrc.2005.06.133.
  • Nishiyama C, Ito T, Nishiyama M, Masaki S, Maeda K, Nakano N, Ng W, Fukuyama K, Yamamoto M, Okumura K, Ogawa H.
    GATA-1 is required for expression of FceRI on mast cells: Analysis of mast cells derived from GATA-1 knockdown mouse bone marrow.
    Int Immunol. 2005 Jul;17(7):847-56. doi: 10.1093/intimm/dxh278.
  • Thongaram T, Hongoh Y, Kosono S, Ohkuma M, Trakulnaleamsai S, Noparatnaraporn N, Kudo T.
    Comparison of bacterial communities in the alkaline gut segment among various species of higher termites.
    Extremophiles. 2005 Jun;9(3):229-38. doi: 10.1007/s00792-005-0440-9.
  • Tsubouchi T, Mineki R, Taka H, Kaga N, Murayama K, Nishiyama C, Yamane H, Kuzuyama T, Nishiyama M.
    Leader peptide-mediated transcriptional attenuation of lysine biosynthetic gene cluster in Thermus thermophilus.
    J Biol Chem. 2005 May 6;280(18):18511-6. doi: 10.1074/jbc.M414456200.
  • Nakano N, Nishiyama C, Masuoka N, Nishiyama M, Yamane H, Okumura K, Ogawa H.
    Analysis of PU.1/ICSBP (IRF-8) complex formation with various PU.1 mutants: molecular cloning of rat Icsbp (Irf-8) cDNA.
    Immunogenetics. 2005 Mar;56(12):871-7. doi: 10.1007/s00251-004-0754-2.
  • Ito T, Nishiyama C, Nishiyama M, Matsuda H, Maeda K, Akizawa Y, Tsuboi R, Okumura K, Ogawa H.
    Mast cells acquire monocyte-specific gene expression and monocyte-like morphology by overproduction of PU.1.
    J Immunol. 2005 Jan 1;174(1):376-83. doi: 10.4049/jimmunol.174.1.376.

2004年

  • Kiribuchi K, Sugimori M, Takeda M, Otani T, Okada K, Onodera H, Ugaki M, Tanaka Y, Tomiyama-Akimoto C, Yamaguchi T, Minami E, Shibuya N, Omori T, Nishiyama M, Nojiri H, Yamane H.
    RERJ1, a jasmonic acid-responsive gene from rice, encodes a basic helix-loop-helix protein.
    Biochem Biophys Res Commun. 2004 Dec 17;325(3):857-63. doi: 10.1016/j.bbrc.2004.10.126.
  • Nishiyama C, Akizawa Y, Nishiyama M, Tokura T, Kawada H, Mitsuishi K, Hasegawa M, Ito T, Nakano N, Okamoto A, Takagi A, Yagita H, Okumura K, Ogawa H.
    Polymorphisms in the Fc epsilon RI beta promoter region affecting transcription activity: a possible promoter-dependent mechanism for association between Fc epsilon RI beta and atopy.
    J Immunol. 2004 Nov 15;173(10):6458-64. doi: 10.4049/jimmunol.173.10.6458.
  • Nishiyama C, Masuoka N, Nishiyama M, Ito T, Yamane H, Okumura K, Ogawa H.
    Evidence against requirement of Ser41 and Ser45 for function of PU.1 -- molecular cloning of rat PU.1.
    FEBS Lett. 2004 Aug 13;572(1-3):57-64. doi: 10.1016/j.febslet.2004.07.008.
  • Nemoto T, Cho EM, Okada A, Okada K, Otomo K, Kanno Y, Toyomasu T, Mitsuhashi W, Sassa T, Minami E, Shibuya N, Nishiyama M, Nojiri H, Yamane H.
    Stemar-13-ene synthase, a diterpene cyclase involved in the biosynthesis of the phytoalexin oryzalexin S in rice.
    FEBS Lett. 2004 Jul 30;571(1-3):182-6. doi: 10.1016/j.febslet.2004.07.002.
  • Jikumaru Y, Asami T, Seto H, Yoshida S, Yokoyama T, Obara N, Hasegawa M, Kodama O, Nishiyama M, Okada K, Nojiri H, Yamane H.
    Preparation and biological activity of molecular probes to identify and analyze jasmonic acid-binding proteins.
    Biosci Biotechnol Biochem. 2004 Jul;68(7):1461-6. doi: 10.1271/bbb.68.1461.
  • Miyazaki T, Miyazaki J, Yamane H, Nishiyama M.
    alpha-Aminoadipate aminotransferase from an extremely thermophilic bacterium, Thermus thermophilus.
    Microbiology. 2004 Jul;150(Pt 7):2327-2334. doi: 10.1099/mic.0.27037-0.
  • Lombo T, Takaya N, Miyazaki J, Gotoh K, Nishiyama M, Kosuge T, Nakamura A, Hoshino T.
    Functional analysis of the small subunit of the putative homoaconitase from Pyrococcus horikoshii in the Thermus lysine pathway.
    FEMS Microbiol Lett. 2004 Apr 15;233(2):315-24. doi: 10.1016/j.femsle.2004.02.026.
  • Nanamiya H, Akanuma G, Natori Y, Murayama R, Kosono S, Kudo T, Kobayashi K, Ogasawara N, Park SM, Ochi K, Kawamura F.
    Zinc is a key factor in controlling alternation of two types of L31 protein in the Bacillus subtilis ribosome.
    Mol Microbiol. 2004 Apr;52(1):273-83. doi: 10.1111/j.1365-2958.2003.03972.x.
  • Kato C, Kurihara T, Kobashi N, Yamane H, Nishiyama M.
    Conversion of feedback regulation in aspartate kinase by domain exchange.
    Biochem Biophys Res Commun. 2004 Apr 9;316(3):802-8. doi: 10.1016/j.bbrc.2004.02.122.
  • Kosono S, Asai K, Sadaie Y, Kudo T.
    Altered gene expression in the transition phase by disruption of a Na+/H+ antiporter gene (shaA) in Bacillus subtilis.
    FEMS Microbiol Lett. 2004 Mar 12;232(1):93-9. doi: 10.1016/S0378-1097(04)00037-0.
  • Nishiyama C, Nishiyama M, Ito T, Masaki S, Masuoka N, Yamane H, Kitamura T, Ogawa H, Okumura K.
    Functional analysis of PU.1 domains in monocyte-specific gene regulation.
    FEBS Lett. 2004 Mar 12;561(1-3):63-8. doi: 10.1016/S0014-5793(04)00116-4.
  • Nishiyama C, Nishiyama M, Ito T, Masaki S, Maeda K, Masuoka N, Yamane H, Kitamura T, Ogawa H, Okumura K.
    Overproduction of PU.1 in mast cell progenitors: its effect on monocyte- and mast cell-specific gene expression.
    Biochem Biophys Res Commun. 2004 Jan 16;313(3):516-21. doi: 10.1016/j.bbrc.2003.11.145.
  • Cho EM, Okada A, Kenmoku H, Otomo K, Toyomasu T, Mitsuhashi W, Sassa T, Yajima A, Yabuta G, Mori K, Oikawa H, Toshima H, Shibuya N, Nojiri H, Omori T, Nishiyama M, Yamane H.
    Molecular cloning and characterization of a cDNA encoding ent-cassa-12,15-diene synthase, a putative diterpenoid phytoalexin biosynthetic enzyme, from suspension-cultured rice cells treated with a chitin elicitor.
    Plant J. 2004 Jan;37(1):1-8. doi: 10.1046/j.1365-313x.2003.01926.x.

2003年

  • Yoshinaka T, Takasu H, Tomizawa R, Kosono S, Kudo T.
    A shaE deletion mutant showed lower Na+ sensitivity compound to other deletion mutants in the Bacillus subtilis sodium/hydrogen antiporter (Sha) system.
    J Biosci Bioeng. 2003;95(3):306-9. doi: 10.1016/s1389-1723(03)80035-x.
  • Sakai H, Vassylyeva MN, Matsuura T, Sekine Si, Gotoh K, Nishiyama M, Terada T, Shirouzu M, Kuramitsu S, Vassylyev DG, Yokoyama S.
    Crystal structure of a lysine biosynthesis enzyme, LysX, from Thermus thermophilus HB8.
    J Mol Biol. 2003 Sep 19;332(3):729-40. doi: 10.1016/s0022-2836(03)00946-x.
  • Vassylyeva MN, Sakai H, Matsuura T, Sekine S, Nishiyama M, Terada T, Shirouzu M, Kuramitsu S, Vassylyev DG, Yokoyama S.
    Cloning, expression, purification, crystallization and initial crystallographic analysis of lysine biosynthesis LysX protein from Thermus thermophilus HB8.
    Acta Crystallogr D Biol Crystallogr. 2003 Sep;59(Pt 9):1651-2. doi: 10.1107/s0907444903014720.
  • Hasegawa M, Nishiyama C, Nishiyama M, Akizawa Y, Mitsuishi K, Ito T, Kawada H, Furukawa S, Ra C, Okumura K, Ogawa H.
    A novel -66T/C polymorphism in FceRI a-chain promoter affecting the transcription activity: possible relationship to allergic diseases.
    J Immunol. 2003 Aug 15;171(4):1927-33. doi: 10.4049/jimmunol.171.4.1927.
  • Nishiyama C, Yokota T, Nishiyama M, Ra C, Okumura K, Ogawa H.
    Molecular cloning of rat YY1 transcription factor.
    Biosci Biotechnol Biochem. 2003 Mar;67(3):654-8. doi: 10.1271/bbb.67.654.
  • Hasegawa M, Nishiyama C, Nishiyama M, Akizawa Y, Mitsuishi K, Ito T, Kawada H, Furukawa S, Ra C, Okumura K, Ogawa H.
    Regulation of the human FceRI alpha-chain distal promoter.
    J Immunol. 2003 Aug 15;171(4):1927-33. doi: 10.4049/jimmunol.171.4.1927.
  • Sobajima H, Takeda M, Sugimori M, Kobashi N, Kiribuchi K, Cho EM, Akimoto C, Yamaguchi T, Minami E, Shibuya N, Schaller F, Weiler EW, Yoshihara T, Nishida H, Nojiri H, Omori T, Nishiyama M, Yamane H.
    Cloning and characterization of a jasmonic acid-responsive gene encoding 12-oxophytodienoic acid reductase in suspension-cultured rice cells.
    Planta. 2003 Feb;216(4):692-8. doi: 10.1007/s00425-002-0909-z.
  • Maeda K, Nishiyama C, Tokura T, Akizawa Y, Nishiyama M, Ogawa H, Okumura K, Ra C.
    Regulation of cell-type specific mouse FceRI beta-chain gene expression by GATA-I via four GATA motifs in the promoter.
    J Immunol. 2003 Jan 1;170(1):334-40. doi: 10.4049/jimmunol.170.1.334.
  • Miyazaki J, Kobashi N, Nishiyama M, Yamane H.
    Characterization of homoisocitrate dehydrogenase involved in lysine biosynthesis of an extremely thermophilic bacterium, Thermus thermophilus HB27, and evolutionary implication of beta-decarboxylating dehydrogenase.
    J Biol Chem. 2003 Jan 17;278(3):1864-71. doi: 10.1074/jbc.M205133200.