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| 2018年4月16日（月）17:00-18:30 東京大学理学部1号館336号室
Yue Him Wong（秋田県立大学、特任助教） |
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| Understanding barnacle shell formation from developmental and molecular perspectives
要旨：Background: Barnacle shell formation begins at the juvenile stage. The timing of biomineralization in barnacle is therefore different from that in sea urchins and mollusks, in which biomineralization begins in the larval stage. The acorn barnacle Megabalanus rosa possesses major fouling problems to the maritime industries in Japan. We sought to understand how barnacles construct their calcified shell plates. In this presentation, I will report our latest observations on the shell formation process of M.rosa and introduce our approach for identifying barnacle shell proteins. Methods: We first investigated the ontology of M.rosa shell formation by video tracking the metamorphosis and early juvenile growth. We then performed microCTscan to visualize mineral deposition during the early juvenile stage. With the developmental data, we proceeded to identify barnacle shell proteins using an integrated transcriptomic and proteomic approach. To begin with, we identified metamorphosing stage and/or early juvenile stage specific transcripts by transcriptomic analyses. In parallel, acid soluble and insoluble adult shell extracts were analyzed using shotgun proteomics. The transcriptome data was then combined with the proteomic data to generate a list of barnacle shell protein candidates. Finally, we performed immunohistochemistry to localize the shell proteins candidates in the early juvenile. Results: In the metamorphosing cyprids, the regions corresponding to the juvenile opercula and juvenile basis are lined with shiny substances, which, based on the microCTscan data, could be mineral deposits. Microtomography further revealed that the basis of juvenile and the base of the developing operculum contain mineral deposits. Our integrated molecular approach identified 60 shell protein candidates, among which 20 are novel proteins containing low complexity repeats and have very high proportion of aspartic acid (D) (30-60%) or glutamine (Q) (20-40%). One of the novel D-rich protein was expressed in the developing operculum. Such expression locality may correlate with the pattern of mineral deposition. Conclusion and future perspectives: Our data indicated that barnacle shell formation is different from other known biomineralization systems, in term of the development and shell protein composition. Our future targets is to identify and characterize the cells responsible for shell formation in different barnacle species. Ultimately, we would like to understand how these cells construct complex and beautiful three-dimensional barnacle shell structures. |
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学会等でバイオミネラルに関連する研究をやっておられる方を見かけ、是非セミナーで発表して頂きたいなどご要望がありましたら、世話人までご連絡ください。 |
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