バイオミネラルゼミ

第13回バイオミネラリゼーションワークショップを開催します。
  • 日時:2018年11月9日(金)13:00-18:00、10日(土)10:00-15:00
  • 場所:東京大学大気海洋研二階講堂 千葉県柏市柏の葉5-1-5
    交通:交通:最寄り駅から東武バス「東大西」で下車。つくばエクスプレス柏の葉キャンパス駅西口から東武バスで6-15分、JR常磐線柏駅西口から東武バスで約25分、東武野田線江戸川台駅東口から東武バスで6-10分

  • アンケートのご協力ありがとうございました。2011年のアンケート結果はこちらでご覧になれます。     
6th International Meeting on Magnetotactic Bacteria 2018のお知らせ
  • 主催:Magnetotactic Bacteria (MTB) 2018 meeting組織委員会
  • 日時:2018年9月10日-14日
  • 場所:金沢商工会議所会館 石川県金沢市尾山町9-13
  • 発表申し込み、参加登録締め切り、6月30日(土)、問い合わ先:sec@mtb2018.jp     

次回セミナー予定
2018年4月16日(月)17:00-18:30 東京大学理学部1号館336号室
Yue Him Wong(秋田県立大学、特任助教)
Understanding barnacle shell formation from developmental and molecular perspectives

要旨:Background: Barnacle shell formation begins at the juvenile stage. The timing of biomineralization in barnacle is therefore different from that in sea urchins and mollusks, in which biomineralization begins in the larval stage. The acorn barnacle Megabalanus rosa possesses major fouling problems to the maritime industries in Japan. We sought to understand how barnacles construct their calcified shell plates. In this presentation, I will report our latest observations on the shell formation process of M.rosa and introduce our approach for identifying barnacle shell proteins. Methods: We first investigated the ontology of M.rosa shell formation by video tracking the metamorphosis and early juvenile growth. We then performed microCTscan to visualize mineral deposition during the early juvenile stage. With the developmental data, we proceeded to identify barnacle shell proteins using an integrated transcriptomic and proteomic approach. To begin with, we identified metamorphosing stage and/or early juvenile stage specific transcripts by transcriptomic analyses. In parallel, acid soluble and insoluble adult shell extracts were analyzed using shotgun proteomics. The transcriptome data was then combined with the proteomic data to generate a list of barnacle shell protein candidates. Finally, we performed immunohistochemistry to localize the shell proteins candidates in the early juvenile. Results: In the metamorphosing cyprids, the regions corresponding to the juvenile opercula and juvenile basis are lined with shiny substances, which, based on the microCTscan data, could be mineral deposits. Microtomography further revealed that the basis of juvenile and the base of the developing operculum contain mineral deposits. Our integrated molecular approach identified 60 shell protein candidates, among which 20 are novel proteins containing low complexity repeats and have very high proportion of aspartic acid (D) (30-60%) or glutamine (Q) (20-40%). One of the novel D-rich protein was expressed in the developing operculum. Such expression locality may correlate with the pattern of mineral deposition. Conclusion and future perspectives: Our data indicated that barnacle shell formation is different from other known biomineralization systems, in term of the development and shell protein composition. Our future targets is to identify and characterize the cells responsible for shell formation in different barnacle species. Ultimately, we would like to understand how these cells construct complex and beautiful three-dimensional barnacle shell structures.
お知らせ
バイオミネラリゼーションセミナーでは幅広い分野の方に発表頂くため、自薦、他薦を問わず広く発表者を募集しております。    
学会等でバイオミネラルに関連する研究をやっておられる方を見かけ、是非セミナーで発表して頂きたいなどご要望がありましたら、世話人までご連絡ください。

Copyright (C) Kogure Laboratory 2011 All Rights Reserved.
design by. (C) WebDaisuki.com