UBI seminar (38th) 2019年10月17日(木)
 場所:東京大学駒場キャンパス 駒場16-107  10:30~
 講演者: Dr. Yusuke Himeoka(Niels Bohr Institute)
 講演タイトル:Degradation of ribosomal RNA during long-term carbon     starvation and phosphorus starvation

概要:Ribosome plays a central role in bacterial growth as the main machinery of the translation process. While it is known that the ribosomes are degraded under the nutrient starvation conditions over timescale of several hours to a few days, the effect of many days of starvation on ribosome level per cell has not been explored. Here, we studied the dynamics of the ribosome level of glucose-starved and phosphorus-starved Escherichia coli cells for up to 30 days. In the glucose starvation, the rRNA per colony forming unit (CFU) dropped to about 30-70% of the value in the exponential growth over 5 days, while after that systematic change was not observed. In the phosphorus starvation, the rRNA per CFU showed a rapid decrease within 2 days to about 10% and stabilized. In order to see whether the differences of the ribosome degradation dynamics are reflected in the resurrection of the bacteria, we measured the starvation time dependence of the lag time in each starvation condition up to the 30 days of starvation. The lag time did not show a clear trend up to 2 weeks of starvation in either condition after subtracting the effect of cell death, while as long-term behavior, the lag time increased with the starvation time.