概要:Ribosome plays a central role in bacterial growth as the main
machinery of the translation process. While it is known that the ribosomes
are degraded under the nutrient starvation conditions over timescale of
several hours to a few days, the effect of many days of starvation on
ribosome level per cell has not been explored. Here, we studied the
dynamics of the ribosome level of glucose-starved and phosphorus-starved
Escherichia coli cells for up to 30 days. In the glucose starvation, the
rRNA per colony forming unit (CFU) dropped to about 30-70% of the value in
the exponential growth over 5 days, while after that systematic change was
not observed. In the phosphorus starvation, the rRNA per CFU showed a
rapid decrease within 2 days to about 10% and stabilized. In order to see
whether the differences of the ribosome degradation dynamics are reflected
in the resurrection of the bacteria, we measured the starvation time
dependence of the lag time in each starvation condition up to the 30 days
of starvation. The lag time did not show a clear trend up to 2 weeks of
starvation in either condition after subtracting the effect of cell death,
while as long-term behavior, the lag time increased with the starvation
time.