東京大学大学院 農学生命科学研究科 応用生命化学専攻
日清食品寄付講座「味覚サイエンス」

Homma, R., Yamashita, H., Funaki, J., Ueda, R., Sakurai, T., Ishimaru, Y., Abe, K., and Asakura, T.
Identification of Bitterness-masking Compounds from Cheese.
J Agric Food Chem. (2012) [Epub ahead of print]
[PubMed]

Ishii, S., Kurokawa, A., Kishi, M., Yamagami, K., Okada, S., Ishimaru, Y., and Misaka, T.
The response of PKD1L3/PKD2L1 to acid stimuli is inhibited by capsaicin and its pungent analogs.
FEBS J. (2012) 279, 1857-1870
[PubMed]

Imai, H., Suzuki, N., Ishimaru, Y., Sakurai, T., Yin, L., Pan, W., Abe, K., Misaka, T., and Hirai, H.
Functional diversity of bitter taste receptor TAS2R16 in primates.
Biol Lett. (2012) [Epub ahead of print]
[PubMed]

Asakura, T., Tamura, T., Terauchi, K., Narikawa, T., Yagasaki, K., Ishimaru, Y., and Abe, K.
Global gene expression profiles in developing soybean seeds.
Plant Physiol. Biochem. (2011) 52, 147-153
[PubMed]

Yamamoto, K., Ishimaru, Y., Ohmoto, M., Matsumoto, I., Asakura, T., and Abe, K.
Genetic tracing of the gustatory neural pathway originating from Pkd1l3-expressing type III taste cells in circumvallate and foliate papillae.
J. Neurochem. (2011) 119, 497-506
[PubMed] [研究紹介]

Asakura, T., Miyano, M., Yamashita, H., Sakurai, T., Nakajima, K., Ito, K., Misaka, T., Ishimaru, Y., and Abe, K.
Analysis of the interaction of food components with model lingual epithelial cells: The case of sweet proteins.
Flavour and Fragrance J. (2011) 26, 274-278

Fujimoto, C., Ishimaru, Y., Katano, Y., Misaka, T., Yamasoba, T., Asakura, T., and Abe, K.
The single pore residue Asp(523) in PKD2L1 determines Ca(2+) permeation of the PKD1L3/PKD2L1 complex.
Biochem. Biophys. Res. Commun. (2011) 404, 946-951
[PubMed]

Nakajima, K., Koizumi, A., Iizuka, K., Ito, K., Morita, Y., Koizumi, T., Asakura, T., Shimizu-Ibuka, A., Misaka, T., and Abe, K.
Non-acidic compounds induce the intense sweet taste of Neoculin, a taste-modifying protein.
Biosci Biotechnol Biochem. (2011) 404, 946-951
[PubMed]

Shibata, M., Ishimatsu-Tsuji, Y., Yokoo, M., Nakai, Y., Abe, K., and Muta-Takada, K.
Effect of oral intake of winged bean extract on a skin lichenification model: evaluation by microarray analysis.
BioFactors. in press

Ueno, Y., Sakurai, T., Okada, S., Abe, K., and Misaka, T.
Human bitter taste receptors hTAS2R8 and hTAS2R39 with differential functions to recognize bitter peptides.
Biosci. Biotechnol. Biochem. (2011) 75, 1188-1190
[PubMed]

Horio, N., Yoshida, R., Yasumatsu, K., Yanagawa, Y., Ishimaru, Y., Matsunami, H., and Ninomiya, Y.
Sour Taste Responses in Mice Lacking PKD Channels.
PLoS One (2011) 6, e20007
[PubMed]

Matsumoto, I., Ohmoto, M., Narukawa, M., Yoshihara, Y., and Abe, K.
Skn-1a (Pou2f3) specifies taste receptor cell lineage.
Nat. Neurosci. (2011) 14, 685-687
[PubMed]

Nakajima, K., Yokoyama. K., Koizumi, T., Koizumi, A., Asakura, T., Terada, T., Masuda, K., Ito, K., Shimizu-Ibuka, A., Misaka, T., and Abe, K.
Identification and Modulation of the Key Amino Acid Residue Responsible for the pH Sensitivity of Neoculin, a Taste-Modifying Protein.
PLoS One (2011) 6, e19448
[PubMed]

Matsumoto, K., Minami, M., Shinozaki, F., Suzuki, Y., Abe, K., Zenno, S., Matsumoto, S., and Minami, Y.
Hsp90 is involved in the formation of P-bodies and stress granules.
Biochem. Biophys. Res. Commun. (2011) 407, 720-724
[PubMed]

Ito, K., Ito, S., Shimamura, T., Weyand, S., Kawarasaki, Y., Misaka, T., Abe, K., Kobayashi, T., Cameron, A. D., and Iwata, S.
Crystal Structure of Glucansucrase from the Dental Caries Pathogen Streptococcus mutans.
J. Mol. Biol. (2011) 408, 177-186
[PubMed]

Izuchi, R., Nakai, Y., Takahashi, H., Ushiama, S., Okada, S., Misaka, T., and Abe, K.
Hepatic gene expression of the insulin signaling pathway is altered by administration of persimmon peel extract: a DNA microarray study using type 2 diabetic Goto-Kakizaki rats.
J. Agric. Food Chem. (2011) 59, 3320-3329
[PubMed]

Ohmoto, M., Okada, S., Nakamura, S., Abe, K., and Matsumoto, I.
Mutually exclusive expression of G?ia and G?14 reveals diversification of taste receptor cells in zebrafish.
J. Comp. Neurol. (2011) 519, 1616-1629
[PubMed]

Nakai, Y., Sato, B., Ushiama, S., Okada, S., Abe, K., and Arai, S.
Hepatic oxidoreduction-related genes are upregulated by administration of hydrogen-saturated drinking water.
Biosci. Biotechnol. Biochem. (2011) 75, 774-776
[PubMed]

Oda, Y., Ueda, F., Kamei, A., Kakinuma, C., and Abe, K.
Biochemical investigation and gene expression analysis of the immunostimulatory functions of an edible Salacia extract in rat small intestine.
BioFactors. (2011) 37, 31-39
[PubMed]

Ito, K., Sugawara, T., Koizumi, A., Nakajima, K. I., Shimizu-Ibuka, A., Shiroishi, M., Asada, H., Yurugi-Kobayashi, T., Shimamura, T., Asakura, T., Misaka, T., Iwata, S., Kobayashi, T., and Abe, K.
Cysteine-to-serine shuffling using a Saccharomyces cerevisiae expression system improves protein secretion: case of a nonglycosylated mutant of miraculin, a taste-modifying protein.
Biotechnol. Lett. (2011) 33, 103-107
[PubMed]

Miyano, M., Yamashita, H., Sakurai, T., Nakajima, K., Ito, K., Misaka, T., Ishimaru, Y., Abe, K., and Asakura, T.
Surface plasmon resonance analysis on interactions of food components with a taste epithelial cell model.
J. Agric. Food Chem. (2010) 58, 11870-11875
[PubMed]

Sakurai, T., Misaka, T., Ueno, Y., Ishiguro, M., Matsuo, S., Ishimaru, Y., Asakura, T., and Abe, K.
The human bitter taste receptor, hTAS2R16, discriminates slight differences in the configuration of disaccharides.
Biochem. Biophys. Res. Commun. (2010) 402, 402595-402601
[PubMed]

Narikawa, T., Tamura, T., Yagasaki, K., Terauchi, K., Sanmiya, K., Ishimaru, Y., Abe, K., and Asakura, T.
Expression of the stress-related genes for glutathione S-transferase and ascorbate peroxidase in the most-glycinin-deficient soybean cultivar Tousan205 during seed maturation.
Biosci. Biotechnol. Biochem. (2010) 74, 1976-1979
[PubMed]

Sakurai, T., Misaka, T., Ishiguro, M., Masuda, K., Sugawara, T., Ito, K., Kobayashi, T., Matsuo, S., Ishimaru, Y., Asakura, T., and Abe, K.
Characterization of the beta-D-glucopyranoside binding site of the human bitter taste receptor hTAS2R16.
J. Biol. Chem. (2010) 285, 28373-28378
[PubMed] [研究紹介]

Ishimaru, Y., Katano, Y., Yamamoto, K., Akiba, M., Misaka, T., Roberts, R. W., Asakura, T., Matsunami, H., and Abe, K.
Interaction between PKD1L3 and PKD2L1 through their transmembrane domains is required for localization of PKD2L1 at taste pores in taste cells of circumvallate and foliate papillae.
FASEB J. (2010) 24, 4058-4067
[PubMed] [研究紹介]

Hata, S., Abe, M., Suzuki, H., Kitamura, F., Toyama-Sorimachi, N., Abe, K., Sakimura, K., and Sorimachi, H.
Calpain 8/nCL-2 and Calpain 9/nCL-4 constitute an active protease complex, G-calpain, involved in gastric mucosal defense.
PLoS Genet. (2010) 6, e1001040
[PubMed]

Ito, K., Sugawara, T., Koizumi, A., Nakajima, K.I., Shimizu-Ibuka, A., Shiroishi, M., Asada, H., Yurugi-Kobayashi, T., Shimamura, T., Asakura, T., Misaka, T., Iwata, S., Kobayashi, T., and Abe, K.
Cysteine-to-serine shuffling using a Saccharomyces cerevisiae expression system improves protein secretion: case of a nonglycosylated mutant of miraculin, a taste-modifying protein.
Biotechnol. Lett. (2010) 33, 103-107
[PubMed]

Funaki, J., Tamura, T., Nishinoaki, M., Misaka, T., Eto, W. and Asakura, T.
Utilization of oryzacystatin for regulating the ripening of squid shiokara, a traditional Japanese salted and fermented seafood.
J. Food Sci. (2010) 75, S527-S530
[PubMed]

Ito, K., Sugawara, T., Koizumi, A., Nakajima, K.I., Shimizu-Ibuka, A., Shiroishi, M., Asada, H., Yurugi-Kobayashi, T., Shimamura, T., Asakura, T., Masuda, K., Ishiguro, M., Misaka, T., Iwata, S., Kobayashi, T., and Abe, K.
Bulky high-mannose-type N-glycan blocks the taste-modifying activity of miraculin.
Biochim. Biophys. Acta.. (2010) 1800, 986-992
[PubMed]

Tamura, T., Kuroda, M., Oikawa, T., Kyozuka, J., Terauchi, K., Ishimaru, Y., Abe, K., and Asakura, T.
Signal peptide peptidases are expressed in the shoot apex of rice, localized to the endoplasmic reticulum.
Plant Cell Rep. (2009) 28, 1615-1621
[PubMed]

Sakurai, T., Misaka,T., Nagai, T., Ishimaru, Y., Matsuo, S., Asakura, T., and Abe, K.
pH-Dependent Inhibition of the Human Bitter Taste Receptor hTAS2R16 by a Variety of Acidic Substances.
J. Agric. Food Chem. (2009) 57, 2508-2514
[PubMed]

Kugino, M., Kugino, K., Tamura, T., and Asakura, T.
Relationship between tissue structural collapse and disappearance of flesh transparency during postmortem changes in squid mantles.
J. Food Sci. (2009) 74, E495-E501
[PubMed]

Morita, Y., Nakajima, K., Iizuka, K., Terada, T., Shimizu-Ibuka, A., Ito, K., Koizumi, A., Asakura, T., Misaka, T., and Abe, K.
pH-dependent structural change in neoculin with special reference to its taste-modifying activity.
Biosci. Biotechnol. Biochem. (2009) 73, 2552-2555
[PubMed]

Matsumoto, I., Asakura, T., Ohmori, T., Tamura, T., and Abe, K.
Cathepsin D-like aspartic proteinase occurring in a maize weevil, Sitophilus zeamais, as its candidate of digestive enzyme.
Biosci. Biotechnol. Biochem. (2009) 73, 2338-2340
[PubMed]

Ishii, S., Misaka, T., Kishi, M., Kaga, T., Ishimaru, Y., and Abe, K.
Acetic acid activates PKD1L3-PKD2L1 channel - a candidate sour taste receptor.
Biochem. Biophys. Res. Commun. (2009) 385, 346-350
[PubMed]

Kiyosaki, T., Asakura, T., Matsumoto, I., Tamura, T., Terauchi, K., Funaki J., Kuroda, M., Misaka, T., and Abe, K.
Wheat cysteine proteases triticain a, b, and c exhibit mutually distinct responses to gibberellin in germinating seeds.
J. Plant Physiol. (2009) 166, 101-106
[PubMed]

Ishimaru, Y. and Matsunami, H.
Transient receptor potential (TRP) channels and taste sensation.
J. Dent. Res. (2009) 88, 212-218
[PubMed]

Ishimaru, Y.
Molecular mechanisms of taste transduction in vertebrates.
Odontology (2009) 97, 1-7
[PubMed]

中島健一郎、古泉文子、朝倉富子、三坂巧
味覚修飾蛋白質ネオクリンの活性化機構と新規甘味蛋白質の開発
蛋白質核酸酵素 (2009) 54, 843-848

Tamura, T., Asakura, T., Uemura, T., Ueda, T., Terauchi, K., Misaka, T., and Abe, K.
Signal peptide peptidase and its homologs in Arabidopsis thaliana--plant tissue-specific expression and distinct subcellular localization.
FEBS J. (2008) 275, 34-43
[PubMed]

Okubo, S., Asakura, T., Okubo, K., Abe, K., Misaka, T., Akita, T., and Abe K.
Neoculin, a taste-modifying sweet protein, accumulates in ripening fruits of cultivated Curculigo latifolia.
J. Plant Physiol. (2008) 165, 1964-1969
[PubMed]

Shimizu-Ibuka, A., Nakai, Y., Nakamori, K., Morita, Y., Nakajima, K., Kadota, K., Watanabe, H., Okubo, S., Terada, T., Asakura, T., Misaka, T., and Abe, K.
Biochemical and Genomic Analysis of Neoculin Compared to Monocot Mannose-Binding Lectins.
J. Agric. Food Chem. (2008) 56, 5338-5344
[PubMed]

Inada, H., Kawabata, F., Ishimaru, Y., Fushiki, T., Matsunami, H., and Tominaga, M.
Off-response property of an acid-activated cation channel complex PKD1L3-PKD2L1.
EMBO Reports (2008) 9, 690-697
[PubMed]

Nakajima, K., Morita, Y., Koizumi, A., Asakura, T., Terada, T., Ito, K., Shimizu-Ibuka, A., Maruyama, J., Kitamoto, K., Misaka, T., and Abe K.
Acid-induced sweetness of neoculin is ascribed to its pH-dependent agonistic-antagonistic interaction with human sweet taste receptor.
FASEB J. (2008) 22, 2323-2330
[PubMed]

Nishinoaki, M., Asakura, T., Watanabe, T., Kunizaki, E., Matusmoto, M., Tamura, T., Minami, M., Obata, A., Abe, K., and Funaki, J.
Application of an Aspergillus saitoi protease preparation to soybean curd to modify its functional and rheological properties.
Biosci. Biotech. Biochem. (2008) 72, 587-590
[PubMed]

Kataoka, S., Yang, R., Ishimaru, Y., Matsunami, H., Sevigny, J., Kinnamon, J. C., and Finger, T. E.
The Candidate Sour Taste Receptor, PKD2L1, Is Expressed by Type III Taste Cells in the Mouse.
Chem. Senses (2008) 33, 243-254
[PubMed]

Nakajima, K., Asakura, T., Misaka, T., and Abe, K.
Neoculin as a novel sweet protein with taste-modifying activity.
Current Topics in Biotechnology. (2008) 4, 75-82

朝倉富子, 田村倫子, 寺内かえで, 阿部啓子
植物のアスパラギン酸プロテアーゼ
化学と生物 (2008) 46, 702-709

石丸喜朗
脊椎動物における味覚受容の分子基盤
実験医学 (2008) 26, 522-528

Tamura, T., Terauchi, K., Kiyosaki T., Asakura, T., Funaki, J., Matsumoto, I., Misaka, T., and Abe, K.
Differential expression of wheat aspartic proteinases, WAP1 and WAP2, in germinating and maturing seeds.
J. Plant Physiol. 164, 470-477.
[PubMed]

Ito, K., Asakura, T., Morita, Y., Nakajima, K., Koizumi, A., Shimizu-Ibuka, A., Masuda, K., Ishiguro, M., Terada, T., Maruyama, J., Kitamoto, K., Misaka, T., and Abe, K.
Microbial production of sensory-active miraculin.
Biochem. Biophys. Res. Commun. 360, 407-411.
[PubMed]

Koizumi, A., Nakajima, K., Asakura, T., Morita, Y., Ito, K., Shmizu-Ibuka, A., Misaka, T., and Abe, K.
Taste-modifying sweet protein, neoculin, is received at human T1R3 amino terminal domain.
Biochem. Biophys. Res. Commun. 358, 585-589.
[PubMed]

Oike, H., Nagai, T., Furuyama, A., Okada, S., Aihara, Y., Ishimaru, Y., Marui, T., Matsumoto, I., Misaka, T., and Abe, K.
Characterization of ligands for fish taste receptors.
J. Neurosci. 27(21), 5584-5592.
[PubMed]

Kiyosaki, T., Matsumoto, I., Asakura, T., Funaki, J., Kuroda, M., Misaka, T., Arai, S., and Abe, K.
Gliadain, a gibberellin-inducible cysteine proteinase occurring in germinating seeds of wheat, Triticum aestivum L., specifically digests gliadin and is regulated by intrinsic cystatins.
FEBS J. 274, 1908-1917.
[PubMed]

Aoki, F., Honda, S., Kishida, H., Kitano, M., Arai, N., Tanaka, H., Yokota, S., Kaku, A., Nakagawa, K., Asakura, T., Nakai, Y., and Mae, T.
Suppression by licorice flavonoids of abdominal fat accumulation and body weight gain in high-fat diet-induced obese C57BL/6J mice.
Biosci. Biotech. Biochem. 71, 206-214.
[PubMed]

Ishimaru, Y., Inada, H., Kubota, M., Zhuang, H., Tominaga, M., and Matsunami, H.
Transient receptor potential family members PKD1L3 and PKD2L1 form a candidate sour taste receptor.
Proc. Natl. Acad. Sci. USA 103(33), 12569-12574.
[PubMed]

Nakajima, K., Asakura, T., Oike, H., Morita, Y., Shimizu-Ibuka, A., Misaka, T., Sorimachi, H., Arai, S., and Abe, K.
Neoculin, a taste-modifying protein, is recognized by human sweet taste receptor.
Neuroreport 17, 1241-1244.
[PubMed]

Terauchi, K., Asakura, T., Ueda, H., Tamura, T., Tamura, K., Matsumoto, I., Misaka, T., Hara-Nishimura, I., and Abe, K.
Plant-specific insertions in the soybean aspartic proteinases, soyAP1 and soyAP2, perform different functions of vacuolar targeting.
J. Plant Physiol. 163, 856-862.
[PubMed]

Nakajima, K., Asakura, T., Maruyama, J., Morita, Y., Oike, H., Shimizu-Ibuka, A., Misaka, T., Sorimachi, H., Arai, S., Kitamoto, K., and Abe, K.
Extracellular production of neoculin, a sweet-tasting heterodimeric protein with taste-modifying activity, by Aspergillus oryzae.
Appl. Environ. Microbiol. 72, 3716-3723.
[PubMed]

Shimizu-Ibuka, A., Morita, Y., Terada, T., Asakura, T., Nakajima, K., Iwata, S., Misaka, T., Sorimachi, H., Arai, S., and Abe, K.
Crystal structure of neoculin: insights into its sweetness and taste-modifying activity.
J. Mol. Biol.. 359, 148-158.
[PubMed]

Ishimaru, Y., Okada, S., Naito, H., Nagai, T., Yasuoka, A., Matsumoto, I., and Abe, K.
Two families of candidate taste receptors in fishes.
Mech. Dev. 122, 1310-1321.
[PubMed]

Nakai, Y., Okada, S., Matsumoto, I., Asakura, T., and Kimura, H.
Time-course characteristics for the digestion of endogenous as well as exogenous protein in the small intestine: a case study with rats fed a [15N]-soy protein isolate.
Biosci. Biotechnol. Biochem. 69, 855-856.
[PubMed]