last update:2019/02/16 15:49:57
Control of protein adsorption to cyclo olefin polymer by the Hofmeister effect.
Fujita R, Nagatoishi S, Adachi S, Nishioka H, Ninomiya H, Kaya T, Takai M, Arakawa T, Tsumoto K
J Pharm Sci 2019 Jan 8Phospholipid Membrane Fluidity Alters Ligand Binding Activity of a G Protein-Coupled Receptor by Shifting the Conformational Equilibrium.
Yoshida K, Nagatoishi S, Kuroda D, Suzuki N, Murata T, Tsumoto K
Biochemistry 2019 Feb 12Generation and characterization of antagonistic anti-human interleukin (IL)-18 monoclonal antibodies with high affinity: Two types of monoclonal antibodies against full-length IL-18 and the neoepitope of inflammatory caspase-cleaved active IL-18.
Nariai Y, Kamino H, Obayashi E, Kato H, Sakashita G, Sugiura T, Migita K, Koga T, Kawakami A, Sakamoto K, Kadomatsu K, Nakakido M, Tsumoto K, Urano T
Arch Biochem Biophys 2019 Jan 4Affinity Improvement of a Cancer-Targeted Antibody through Alanine-Induced Adjustment of Antigen-Antibody Interface.
Yamashita T, Mizohata E, Nagatoishi S, Watanabe T, Nakakido M, Iwanari H, Mochizuki Y, Nakayama T, Kado Y, Yokota Y, Matsumura H, Kawamura T, Kodama T, Hamakubo T, Inoue T, Fujitani H, Tsumoto K
Structure 2018 Nov 20Thermodynamic and computational analyses reveal the functional roles of the galloyl group of tea catechins in molecular recognition.
Takahashi T, Nagatoishi S, Kuroda D, Tsumoto K
PLoS One 2018Molecular basis for governing the morphology of type-I collagen fibrils by Osteomodulin.
Tashima T, Nagatoishi S, Caaveiro JMM, Nakakido M, Sagara H, Kusano-Arai O, Iwanari H, Mimuro H, Hamakubo T, Ohnuma SI, Tsumoto K
Commun Biol 2018Antibody Affinity Maturation by Computational Design.
Kuroda D, Tsumoto K
Methods Mol Biol 2018Structural behavior of keratin-associated protein 8.1 in human hair as revealed by a monoclonal antibody.
Akiba H, Ikeuchi E, Ganbat J, Fujikawa H, Arai-Kusano O, Iwanari H, Nakakido M, Hamakubo T, Shimomura Y, Tsumoto K
J Struct Biol 2018 NovImprovement of antibody affinity by introduction of basic amino acid residues into the framework region.
Fukunaga A, Maeta S, Reema B, Nakakido M, Tsumoto K
Biochem Biophys Rep 2018 Sep[Biophysical Analysis of the Protein-Small Molecule Interactions to Develop Small Molecule Drug Discovery].
Nagatoishi S, Caaveiro JMM, Tsumoto K
Yakugaku Zasshi 2018Discovery and Optimization of Inhibitors of the Parkinson's Disease Associated Protein DJ-1.
Tashiro S, Caaveiro JMM, Nakakido M, Tanabe A, Nagatoishi S, Tamura Y, Matsuda N, Liu D, Hoang QQ, Tsumoto K
ACS Chem Biol 2018 Sep 21Long-Term Stability and Reversible Thermal Unfolding of Antibody Structure at Low pH: Case Study.
Fukada H, Tsumoto K, Arakawa T, Ejima D
J Pharm Sci 2018 NovTyrosine Sulfation Restricts the Conformational Ensemble of a Flexible Peptide, Strengthening the Binding Affinity for an Antibody.
Miyanabe K, Yamashita T, Abe Y, Akiba H, Takamatsu Y, Nakakido M, Hamakubo T, Ueda T, Caaveiro JMM, Tsumoto K
Biochemistry 2018 Jul 17Intramolecular H-bonds govern the recognition of a flexible peptide by an antibody.
Miyanabe K, Akiba H, Kuroda D, Nakakido M, Kusano-Arai O, Iwanari H, Hamakubo T, Caaveiro JMM, Tsumoto K
J Biochem 2018 Jul 1Roles of the disulfide bond between the variable and the constant domains of rabbit immunoglobulin kappa chains in thermal stability and affinity.
Kawade R, Akiba H, Entzminger K, Maruyama T, Okumura CJ, Tsumoto K
Protein Eng Des Sel 2018 Jul 1Inhibition of homophilic dimerization and disruption of cell adhesion by P-cadherin-specific small molecules from SPR-based assays.
Senoo A, Nagatoishi S, Moberg A, Babol LN, Mitani T, Tashima T, Kudo S, Tsumoto K
Chem Commun (Camb) 2018 May 22Repertoire Analysis of Antibody CDR-H3 Loops Suggests Affinity Maturation Does Not Typically Result in Rigidification.
Jeliazkov JR, Sljoka A, Kuroda D, Tsuchimura N, Katoh N, Tsumoto K, Gray JJ
Front Immunol 2018A combination of 19F NMR and surface plasmon resonance for site-specific hit selection and validation of fragment molecules that bind to the ATP-binding site of a kinase.
Nagatoishi S, Yamaguchi S, Katoh E, Kajita K, Yokotagawa T, Kanai S, Furuya T, Tsumoto K
Bioorg Med Chem 2018 May 1Assessing the Heterogeneity of the Fc-Glycan of a Therapeutic Antibody Using an engineered FcγReceptor IIIa-Immobilized Column.
Kiyoshi M, Caaveiro JMM, Tada M, Tamura H, Tanaka T, Terao Y, Morante K, Harazono A, Hashii N, Shibata H, Kuroda D, Nagatoishi S, Oe S, Ide T, Tsumoto K, Ishii-Watabe A
Sci Rep 2018 Mar 2Synergistic Cytotoxic Effect on Gastric Cancer Cells of an Immunotoxin Cocktail in Which Antibodies Recognize Different Epitopes on CDH17.
Kusano-Arai O, Iwanari H, Kudo S, Kikuchi C, Yui A, Akiba H, Matsusaka K, Kaneda A, Fukayama M, Tsumoto K, Hamakubo T
Monoclon Antib Immunodiagn Immunother 2018 FebStructures of the prefusion form of measles virus fusion protein in complex with inhibitors.
Hashiguchi T, Fukuda Y, Matsuoka R, Kuroda D, Kubota M, Shirogane Y, Watanabe S, Tsumoto K, Kohda D, Plemper RK, Yanagi Y
Proc Natl Acad Sci U S A 2018 Mar 6Development of drug discovery screening system by molecular interaction kinetics-mass spectrometry.
Obi N, Fukuda T, Nakayama N, Ervin J, Bando Y, Nishimura T, Nagatoishi S, Tsumoto K, Kawamura T
Rapid Commun Mass Spectrom 2018 Apr 30A secondary RET mutation in the activation loop conferring resistance to vandetanib.
Nakaoku T, Kohno T, Araki M, Niho S, Chauhan R, Knowles PP, Tsuchihara K, Matsumoto S, Shimada Y, Mimaki S, Ishii G, Ichikawa H, Nagatoishi S, Tsumoto K, Okuno Y, Yoh K, McDonald NQ, Goto K
Nat Commun 2018 Feb 12Characterization of glycoengineered anti-HER2 monoclonal antibodies produced by using a silkworm-baculovirus expression system.
Egashira Y, Nagatoishi S, Kiyoshi M, Ishii-Watabe A, Tsumoto K
J Biochem 2018 Jun 1Enzymatically cleavable traceless biotin tags for protein PEGylation and purification.
Wawro AM, Aoki Y, Muraoka T, Tsumoto K, Kinbara K
Chem Commun (Camb) 2018 Feb 15Production and characterization of a novel site-specific-modifiable anti-OX40-receptor single-chain variable fragment for targeted drug delivery.
Tanabe A, Nakano K, Nakakido M, Nagatoishi S, Tanaka Y, Tsumoto K, Uchimaru K, Watanabe T
Biochem Biophys Res Commun 2018 Feb 5Introduction: Antibody-Mediated Therapy Special Issue Part 2.
Nakamura A, Tsumoto K, Takai T
Int Immunol 2017 Dec 30PRDM14 directly interacts with heat shock proteins HSP90α and glucose-regulated protein 78.
Moriya C, Taniguchi H, Nagatoishi S, Igarashi H, Tsumoto K, Imai K
Cancer Sci 2018 FebThermodynamic analyses of amino acid residues at the interface of an antibody B2212A and its antigen roundabout homolog 1.
Yui A, Akiba H, Kudo S, Nakakido M, Nagatoishi S, Tsumoto K
J Biochem 2017 Oct 1Analysis of Pore Formation and Protein Translocation Using Large Biological Nanopores.
Watanabe H, Gubbiotti A, Chinappi M, Takai N, Tanaka K, Tsumoto K, Kawano R
Anal Chem 2017 Nov 7Structure of the triose-phosphate/phosphate translocator reveals the basis of substrate specificity.
Lee Y, Nishizawa T, Takemoto M, Kumazaki K, Yamashita K, Hirata K, Minoda A, Nagatoishi S, Tsumoto K, Ishitani R, Nureki O
Nat Plants 2017 OctPolymeric SpyCatcher Scaffold Enables Bioconjugation in a Ratio-Controllable Manner.
Jia L, Minamihata K, Ichinose H, Tsumoto K, Kamiya N
Biotechnol J 2017 DecBiophysical characterization of the interaction between heme and proteins responsible for heme transfer in Streptococcus pyogenes.
Hoshino M, Nakakido M, Nagatoishi S, Aikawa C, Nakagawa I, Tsumoto K
Biochem Biophys Res Commun 2017 Nov 18Structural basis for binding and transfer of heme in bacterial heme-acquisition systems.
Naoe Y, Nakamura N, Rahman MM, Tosha T, Nagatoishi S, Tsumoto K, Shiro Y, Sugimoto H
Proteins 2017 DecElucidation of potential sites for antibody engineering by fluctuation editing.
Yanaka S, Moriwaki Y, Tsumoto K, Sugase K
Sci Rep 2017 Aug 30Insight in the Evolution of Therapeutic Monoclonal Antibodies-Fundamental Technologies for Creation and Evaluation of Next Generation mAbs.
Ishii-Watabe A, Tsumoto K
Yakugaku Zasshi 2017Glycosylation of IgG-Fc: a molecular perspective.
Kiyoshi M, Tsumoto K, Ishii-Watabe A, Caaveiro JMM
Int Immunol 2017 Jul 1Use of SpyTag/SpyCatcher to construct bispecific antibodies that target two epitopes of a single antigen.
Yumura K, Akiba H, Nagatoishi S, Kusano-Arai O, Iwanari H, Hamakubo T, Tsumoto K
J Biochem 2017 Sep 1Haemolytic actinoporins interact with carbohydrates using their lipid-binding module.
Tanaka K, Caaveiro JMM, Morante K, Tsumoto K
Philos Trans R Soc Lond B Biol Sci 2017 Aug 5Functional Contacts between MPER and the Anti-HIV-1 Broadly Neutralizing Antibody 4E10 Extend into the Core of the Membrane.
Rujas E, Insausti S, García-Porras M, Sánchez-Eugenia R, Tsumoto K, Nieva JL, Caaveiro JM
J Mol Biol 2017 Apr 21Peripheral Membrane Interactions Boost the Engagement by an Anti-HIV-1 Broadly Neutralizing Antibody.
Rujas E, Caaveiro JM, Insausti S, García-Porras M, Tsumoto K, Nieva JL
J Biol Chem 2017 Mar 31The carboxyl-terminal region of Dok-7 plays a key, but not essential, role in activation of muscle-specific receptor kinase MuSK and neuromuscular synapse formation.
Ueta R, Tezuka T, Izawa Y, Miyoshi S, Nagatoishi S, Tsumoto K, Yamanashi Y
J Biochem 2017 Mar 1Disruption of cell adhesion by an antibody targeting the cell-adhesive intermediate (X-dimer) of human P-cadherin.
Kudo S, Caaveiro JM, Nagatoishi S, Miyafusa T, Matsuura T, Sudou Y, Tsumoto K
Sci Rep 2017 Jan 3Differential Effect of Membrane Composition on the Pore-Forming Ability of Four Different Sea Anemone Actinoporins.
García-Linares S, Rivera-de-Torre E, Morante K, Tsumoto K, Caaveiro JM, Gavilanes JG, Slotte JP, Martínez-Del-Pozo Á
Biochemistry 2016 Dec 6Structural basis for broad neutralization of HIV-1 through the molecular recognition of 10E8 helical epitope at the membrane interface.
Rujas E, Caaveiro JM, Partida-Hanon A, Gulzar N, Morante K, Apellániz B, García-Porras M, Bruix M, Tsumoto K, Scott JK, Jiménez MÁ, Nieva JL
Sci Rep 2016 Dec 1Through-bond effects in the ternary complexes of thrombin sandwiched by two DNA aptamers.
Pica A, Russo Krauss I, Parente V, Tateishi-Karimata H, Nagatoishi S, Tsumoto K, Sugimoto N, Sica F
Nucleic Acids Res 2017 Jan 9Production and characterization of genetically modified human IL-11 variants.
Sano E, Takei T, Ueda T, Tsumoto K
Biochim Biophys Acta Gen Subj 2017 FebImproved Brain Expression of Anti-Amyloid β scFv by Complexation of mRNA Including a Secretion Sequence with PEG-based Block Catiomer.
Perche F, Uchida S, Akiba H, Lin CY, Ikegami M, Dirisala A, Nakashima T, Itaka K, Tsumoto K, Kataoka K
Curr Alzheimer Res 2017Adhesive Dimerization of Human P-Cadherin Catalyzed by a Chaperone-like Mechanism.
Kudo S, Caaveiro JM, Tsumoto K
Structure 2016 Sep 6In-Cell Enzymology To Probe His-Heme Ligation in Heme Oxygenase Catalysis.
Sigala PA, Morante K, Tsumoto K, Caaveiro JM, Goldberg DE
Biochemistry 2016 Aug 30Identification of a Membrane-bound Prepore Species Clarifies the Lytic Mechanism of Actinoporins.
Morante K, Bellomio A, Gil-Cartón D, Redondo-Morata L, Sot J, Scheuring S, Valle M, González-Mañas JM, Tsumoto K, Caaveiro JMM
J Biol Chem 2016 Sep 9Structural basis for amino acid export by DMT superfamily transporter YddG.
Tsuchiya H, Doki S, Takemoto M, Ikuta T, Higuchi T, Fukui K, Usuda Y, Tabuchi E, Nagatoishi S, Tsumoto K, Nishizawa T, Ito K, Dohmae N, Ishitani R, Nureki O
Nature 2016 Jun 16Quantitative phosphoproteomics-based molecular network description for high-resolution kinase-substrate interactome analysis.
Narushima Y, Kozuka-Hata H, Tsumoto K, Inoue J, Oyama M
Bioinformatics 2016 Jul 15Reply to "The Broadly Neutralizing, Anti-HIV Antibody 4E10: an Open and Shut Case?".
Rujas E, Gulzar N, Morante K, Tsumoto K, Scott JK, Nieva JL, Caaveiro JM
J Virol 2016 MarAssessment of the Protein-Protein Interactions in a Highly Concentrated Antibody Solution by Using Raman Spectroscopy.
Ota C, Noguchi S, Nagatoishi S, Tsumoto K
Pharm Res 2016 AprIntegrative Network Analysis Combined with Quantitative Phosphoproteomics Reveals Transforming Growth Factor-beta Receptor type-2 (TGFBR2) as a Novel Regulator of Glioblastoma Stem Cell Properties.
Narushima Y, Kozuka-Hata H, Koyama-Nasu R, Tsumoto K, Inoue J, Akiyama T, Oyama M
Mol Cell Proteomics 2016 MarRapid Heme Transfer Reactions between NEAr Transporter Domains of Staphylococcus aureus: A Theoretical Study Using QM/MM and MD Simulations.
Moriwaki Y, Terada T, Tsumoto K, Shimizu K
PLoS One 2015Epiregulin Recognition Mechanisms by Anti-epiregulin Antibody 9E5: STRUCTURAL, FUNCTIONAL, AND MOLECULAR DYNAMICS SIMULATION ANALYSES.
Kado Y, Mizohata E, Nagatoishi S, Iijima M, Shinoda K, Miyafusa T, Nakayama T, Yoshizumi T, Sugiyama A, Kawamura T, Lee YH, Matsumura H, Doi H, Fujitani H, Kodama T, Shibasaki Y, Tsumoto K, Inoue T
J Biol Chem 2016 Jan 29Mitochonic Acid 5 Binds Mitochondria and Ameliorates Renal Tubular and Cardiac Myocyte Damage.
Suzuki T, Yamaguchi H, Kikusato M, Hashizume O, Nagatoishi S, Matsuo A, Sato T, Kudo T, Matsuhashi T, Murayama K, Ohba Y, Watanabe S, Kanno S, Minaki D, Saigusa D, Shinbo H, Mori N, Yuri A, Yokoro M, Mishima E, Shima H, Akiyama Y, Takeuchi Y, Kikuchi K, Toyohara T, Suzuki C, Ichimura T, Anzai J, Kohzuki M, Mano N, Kure S, Yanagisawa T, Tomioka Y, Toyomizu M, Tsumoto K, Nakada K, Bonventre JV, Ito S, Osaka H, Hayashi K, Abe T
J Am Soc Nephrol 2016 JulBidirectional Transformation of a Metamorphic Protein between the Water-Soluble and Transmembrane Native States.
Tanaka K, Caaveiro JM, Tsumoto K
Biochemistry 2015 Nov 24Structural basis for inhibition of the Tob-CNOT7 interaction by a fragment screening approach.
Bai Y, Tashiro S, Nagatoishi S, Suzuki T, Yan D, Liu R, Tsumoto K, Bartlam M, Yamamoto T
Protein Cell 2015 DecStructural analysis of Fc/FcγR complexes: a blueprint for antibody design.
Caaveiro JM, Kiyoshi M, Tsumoto K
Immunol Rev 2015 NovDiscovery and characterization of natural tropolones as inhibitors of the antibacterial target CapF from Staphylococcus aureus.
Nakano K, Chigira T, Miyafusa T, Nagatoishi S, Caaveiro JM, Tsumoto K
Sci Rep 2015 Oct 16Structural and Thermodynamic Basis of Epitope Binding by Neutralizing and Nonneutralizing Forms of the Anti-HIV-1 Antibody 4E10.
Rujas E, Gulzar N, Morante K, Tsumoto K, Scott JK, Nieva JL, Caaveiro JM
J Virol 2015 DecDifferential Effects of IFN-β on the Survival and Growth of Human Vascular Smooth Muscle and Endothelial Cells.
Sano E, Tashiro S, Tsumoto K, Ueda T
Biores Open Access 2015Thermodynamic characterization of the interaction between the human Y-box binding protein YB-1 and nucleic acids.
Tanabe Y, Nagatoishi S, Tsumoto K
Mol Biosyst 2015 SepFunctional characterization of Val60, a key residue involved in the membrane-oligomerization of fragaceatoxin C, an actinoporin from Actinia fragacea.
Morante K, Caaveiro JM, Viguera AR, Tsumoto K, González-Mañas JM
FEBS Lett 2015 Jul 8Differential binding of prohibitin-2 to estrogen receptor α and to drug-resistant ERα mutants.
Chigira T, Nagatoishi S, Tsumoto K
Biochem Biophys Res Commun 2015 Aug 7Osteomodulin regulates diameter and alters shape of collagen fibrils.
Tashima T, Nagatoishi S, Sagara H, Ohnuma S, Tsumoto K
Biochem Biophys Res Commun 2015 Jul 31Thermodynamics of antibody-antigen interaction revealed by mutation analysis of antibody variable regions.
Akiba H, Tsumoto K
J Biochem 2015 JulStructural basis for binding of human IgG1 to its high-affinity human receptor FcγRI.
Kiyoshi M, Caaveiro JM, Kawai T, Tashiro S, Ide T, Asaoka Y, Hatayama K, Tsumoto K
Nat Commun 2015 Apr 30Protein stabilization by an amphiphilic short monodisperse oligo(ethylene glycol).
Sadhukhan N, Muraoka T, Ui M, Nagatoishi S, Tsumoto K, Kinbara K
Chem Commun (Camb) 2015 May 18The Atomic Structure of the HIV-1 gp41 Transmembrane Domain and Its Connection to the Immunogenic Membrane-proximal External Region.
Apellániz B, Rujas E, Serrano S, Morante K, Tsumoto K, Caaveiro JM, Jiménez MÁ, Nieva JL
J Biol Chem 2015 May 22A pore-forming toxin requires a specific residue for its activity in membranes with particular physicochemical properties.
Morante K, Caaveiro JM, Tanaka K, González-Mañas JM, Tsumoto K
J Biol Chem 2015 Apr 24Click conjugation of a binuclear terbium(III) complex for real-time detection of tyrosine phosphorylation.
Akiba H, Sumaoka J, Tsumoto K, Komiyama M
Anal Chem 2015 Apr 7Structural basis for self-assembly of a cytolytic pore lined by protein and lipid.
Tanaka K, Caaveiro JM, Morante K, González-Mañas JM, Tsumoto K
Nat Commun 2015 Feb 26pH responsiveness of fibrous assemblies of repeat-sequence amphipathic α-helix polypeptides.
Takei T, Tsumoto K, Okonogi A, Kimura A, Kojima S, Yazaki K, Takei T, Ueda T, Miura K
Protein Sci 2015 MayRevealing the peptide presenting process of human leukocyte antigen through the analysis of fluctuation.
Yanaka S, Ueno T, Tsumoto K, Sugase K
Biophysics (Nagoya-shi) 2015Structural features of interfacial tyrosine residue in ROBO1 fibronectin domain-antibody complex: Crystallographic, thermodynamic, and molecular dynamic analyses.
Nakayama T, Mizohata E, Yamashita T, Nagatoishi S, Nakakido M, Iwanari H, Mochizuki Y, Kado Y, Yokota Y, Satoh R, Tsumoto K, Fujitani H, Kodama T, Hamakubo T, Inoue T
Protein Sci 2015 MarQuantitative laser diffraction method for the assessment of protein subvisible particles.
Totoki S, Yamamoto G, Tsumoto K, Uchiyama S, Fukui K
J Pharm Sci 2015 FebCrystal structure of streptavidin mutant with low immunogenicity.
Kawato T, Mizohata E, Meshizuka T, Doi H, Kawamura T, Matsumura H, Yumura K, Tsumoto K, Kodama T, Inoue T, Sugiyama A
J Biosci Bioeng 2015 JunThe molecular interaction of a protein in highly concentrated solution investigated by Raman spectroscopy.
Ota C, Noguchi S, Tsumoto K
Biopolymers 2015 AprEfficacy of ribavirin against malignant glioma cell lines.
Ogino A, Sano E, Ochiai Y, Yamamuro S, Tashiro S, Yachi K, Ohta T, Fukushima T, Okamoto Y, Tsumoto K, Ueda T, Yoshino A, Katayama Y
Oncol Lett 2014 DecPreparation and characterization of anti-tissue factor single-chain variable fragment antibody for cancer diagnosis.
Sato R, Obonai T, Tsumura R, Tsumoto K, Koga Y, Yasunaga M, Matsumura Y
Cancer Sci 2014 DecReversible ion transportation switch by a ligand-gated synthetic supramolecular ion channel.
Muraoka T, Endo T, Tabata KV, Noji H, Nagatoishi S, Tsumoto K, Li R, Kinbara K
J Am Chem Soc 2014 Nov 5Recent advances in antibody engineering.
Tsumoto K, Hagihara Y, Saerens D
Biochim Biophys Acta 2014 NovGrafting synthetic transmembrane units to the engineered low-toxicity α-hemolysin to restore its hemolytic activity.
Ui M, Harima K, Takei T, Tsumoto K, Tabata KV, Noji H, Endo S, Akiyama K, Muraoka T, Kinbara K
Mol Biosyst 2014 DecEffects of syringe material and silicone oil lubrication on the stability of pharmaceutical proteins.
Krayukhina E, Tsumoto K, Uchiyama S, Fukui K
J Pharm Sci 2015 FebProtective effect of the long pentraxin PTX3 against histone-mediated endothelial cell cytotoxicity in sepsis.
Daigo K, Nakakido M, Ohashi R, Fukuda R, Matsubara K, Minami T, Yamaguchi N, Inoue K, Jiang S, Naito M, Tsumoto K, Hamakubo T
Sci Signal 2014 Sep 16Peptide-dependent conformational fluctuation determines the stability of the human leukocyte antigen class I complex.
Yanaka S, Ueno T, Shi Y, Qi J, Gao GF, Tsumoto K, Sugase K
J Biol Chem 2014 Aug 29Next generation drying technologies for pharmaceutical applications.
Walters RH, Bhatnagar B, Tchessalov S, Izutsu KI, Tsumoto K, Ohtake S
J Pharm Sci 2014 SepAlternative downstream processes for production of antibodies and antibody fragments.
Arakawa T, Tsumoto K, Ejima D
Biochim Biophys Acta 2014 NovThe staphylococcal elastin-binding protein regulates zinc-dependent growth/biofilm formation.
Nakakido M, Aikawa C, Nakagawa I, Tsumoto K
J Biochem 2014 SepThermodynamic and structural characterization of the specific binding of Zn(II) to human protein DJ-1.
Tashiro S, Caaveiro JM, Wu CX, Hoang QQ, Tsumoto K
Biochemistry 2014 Apr 15Role of positions e and g in the fibrous assembly formation of an amphipathic α-helix-forming polypeptide.
Takei T, Tsumoto K, Yoshino M, Kojima S, Yazaki K, Ueda T, Takei T, Arisaka F, Miura K
Biopolymers 2014 MayIdentification and characterization of the X-dimer of human P-cadherin: implications for homophilic cell adhesion.
Kudo S, Caaveiro JM, Goda S, Nagatoishi S, Ishii K, Matsuura T, Sudou Y, Kodama T, Hamakubo T, Tsumoto K
Biochemistry 2014 Mar 25Affinity improvement of a therapeutic antibody by structure-based computational design: generation of electrostatic interactions in the transition state stabilizes the antibody-antigen complex.
Kiyoshi M, Caaveiro JM, Miura E, Nagatoishi S, Nakakido M, Soga S, Shirai H, Kawabata S, Tsumoto K
PLoS One 2014Heme binding mechanism of structurally similar iron-regulated surface determinant near transporter domains of Staphylococcus aureus exhibiting different affinities for heme.
Moriwaki Y, Terada T, Caaveiro JM, Takaoka Y, Hamachi I, Tsumoto K, Shimizu K
Biochemistry 2013 Dec 10Improving the affinity of an antibody for its antigen via long-range electrostatic interactions.
Fukunaga A, Tsumoto K
Protein Eng Des Sel 2013 DecDynamic elements govern the catalytic activity of CapE, a capsular polysaccharide-synthesizing enzyme from Staphylococcus aureus.
Miyafusa T, Caaveiro JM, Tanaka Y, Tsumoto K
FEBS Lett 2013 Nov 29Discovery of an uncovered region in fibrin clots and its clinical significance.
Hisada Y, Yasunaga M, Hanaoka S, Saijou S, Sugino T, Tsuji A, Saga T, Tsumoto K, Manabe S, Kuroda J, Kuratsu J, Matsumura Y
Sci Rep 2013Effects of subclass change on the structural stability of chimeric, humanized, and human antibodies under thermal stress.
Ito T, Tsumoto K
Protein Sci 2013 NovHyperthin nanochains composed of self-polymerizing protein shackles.
Matsunaga R, Yanaka S, Nagatoishi S, Tsumoto K
Nat Commun 2013Bidirectional binding property of high glycine-tyrosine keratin-associated protein contributes to the mechanical strength and shape of hair.
Matsunaga R, Abe R, Ishii D, Watanabe SI, Kiyoshi M, Nöcker B, Tsuchiya M, Tsumoto K
J Struct Biol 2013 SepSelective binding of antimicrobial porphyrins to the heme-receptor IsdH-NEAT3 of Staphylococcus aureus.
Vu NT, Moriwaki Y, Caaveiro JM, Terada T, Tsutsumi H, Hamachi I, Shimizu K, Tsumoto K
Protein Sci 2013 JulCrystal structure of the capsular polysaccharide synthesizing protein CapE of Staphylococcus aureus.
Miyafusa T, Caaveiro JM, Tanaka Y, Tanner ME, Tsumoto K
Biosci Rep 2013 Jun 11Heme degradation by Staphylococcus aureus IsdG and IsdI liberates formaldehyde rather than carbon monoxide.
Matsui T, Nambu S, Ono Y, Goulding CW, Tsumoto K, Ikeda-Saito M
Biochemistry 2013 May 7Effects of chain length of an amphipathic polypeptide carrying the repeated amino acid sequence (LETLAKA)(n) on α-helix and fibrous assembly formation.
Takei T, Hasegawa K, Imada K, Namba K, Tsumoto K, Kuriki Y, Yoshino M, Yazaki K, Kojima S, Takei T, Ueda T, Miura K
Biochemistry 2013 Apr 23Incorporation of rapid thermodynamic data in fragment-based drug discovery.
Kobe A, Caaveiro JM, Tashiro S, Kajihara D, Kikkawa M, Mitani T, Tsumoto K
J Med Chem 2013 Mar 14Identification of small-molecule inhibitors of the human S100B-p53 interaction and evaluation of their activity in human melanoma cells.
Yoshimura C, Miyafusa T, Tsumoto K
Bioorg Med Chem 2013 Mar 1Secretory production of single-chain antibody (scFv) in Brevibacillus choshinensis using novel fusion partner.
Tokunaga M, Mizukami M, Yamasaki K, Tokunaga H, Onishi H, Hanagata H, Ishibashi M, Miyauchi A, Tsumoto K, Arakawa T
Appl Microbiol Biotechnol 2013 OctMutations for decreasing the immunogenicity and maintaining the function of core streptavidin.
Yumura K, Ui M, Doi H, Hamakubo T, Kodama T, Tsumoto K, Sugiyama A
Protein Sci 2013 FebCrystal structure of the C-terminal domain of Mu phage central spike and functions of bound calcium ion.
Harada K, Yamashita E, Nakagawa A, Miyafusa T, Tsumoto K, Ueno T, Toyama Y, Takeda S
Biochim Biophys Acta 2013 JanPhosphoproteome of human glioblastoma initiating cells reveals novel signaling regulators encoded by the transcriptome.
Kozuka-Hata H, Nasu-Nishimura Y, Koyama-Nasu R, Ao-Kondo H, Tsumoto K, Akiyama T, Oyama M
PLoS One 2012Type 1 IFN inhibits the growth factor deprived apoptosis of cultured human aortic endothelial cells and protects the cells from chemically induced oxidative cytotoxicity.
Sano E, Tashiro S, Tadakuma H, Takei T, Ueda T, Tsumoto K
J Cell Biochem 2012 DecThermodynamic analysis of a multifunctional RNA-binding protein, PhoRpp38, in the hyperthermophilic archaeon Pyrococcus horikoshii OT3.
Oshima K, Nakashima T, Kakuta Y, Tsumoto K, Kimura M
Biosci Biotechnol Biochem 2012Structural and thermodynamic characterization of the self-adhesive properties of human P-cadherin.
Kudo S, Caaveiro JM, Miyafusa T, Goda S, Ishii K, Matsuura T, Sudou Y, Kodama T, Hamakubo T, Tsumoto K
Mol Biosyst 2012 AugApplication of photoactive yellow protein as a photoresponsive module for controlling hemolytic activity of staphylococcal α-hemolysin.
Ui M, Tanaka Y, Araki Y, Wada T, Takei T, Tsumoto K, Endo S, Kinbara K
Chem Commun (Camb) 2012 May 16Establishment of a microcarrier culture system with serial sub-cultivation for functionally active human endothelial cells.
Tashiro S, Tsumoto K, Sano E
J Biotechnol 2012 Aug 31Mapping ultra-weak protein-protein interactions between heme transporters of Staphylococcus aureus.
Abe R, Caaveiro JM, Kozuka-Hata H, Oyama M, Tsumoto K
J Biol Chem 2012 May 11Crystal structure of the enzyme CapF of Staphylococcus aureus reveals a unique architecture composed of two functional domains.
Miyafusa T, Caaveiro JM, Tanaka Y, Tsumoto K
Biochem J 2012 May 1Interleukin-11 links oxidative stress and compensatory proliferation.
Nishina T, Komazawa-Sakon S, Yanaka S, Piao X, Zheng DM, Piao JH, Kojima Y, Yamashina S, Sano E, Putoczki T, Doi T, Ueno T, Ezaki J, Ushio H, Ernst M, Tsumoto K, Okumura K, Nakano H
Sci Signal 2012 Jan 17Catalytic activity of MsbA reconstituted in nanodisc particles is modulated by remote interactions with the bilayer.
Kawai T, Caaveiro JM, Abe R, Katagiri T, Tsumoto K
FEBS Lett 2011 Nov 16Gene expression profiles predicting the response to IFN-β and a combination of temozolomide and IFN-β in malignant gliomas.
Yoshino A, Tashiro S, Ogino A, Yachi K, Ohta T, Fukushima T, Watanabe T, Katayama Y, Okamoto Y, Sano E, Tsumoto K
Int J Oncol 2011 SepMolecular basis of recognition of antibacterial porphyrins by heme-transporter IsdH-NEAT3 of Staphylococcus aureus.
Moriwaki Y, Caaveiro JM, Tanaka Y, Tsutsumi H, Hamachi I, Tsumoto K
Biochemistry 2011 Aug 30Hydration is required in DNA G-quadruplex-protein binding.
Nagatoishi S, Isono N, Tsumoto K, Sugimoto N
Chembiochem 2011 Aug 16Monoubiquitination of Tob/BTG family proteins competes with degradation-targeting polyubiquitination.
Suzuki T, Kim M, Kozuka-Hata H, Watanabe M, Oyama M, Tsumoto K, Yamamoto T
Biochem Biophys Res Commun 2011 May 27Loop residues of thrombin-binding DNA aptamer impact G-quadruplex stability and thrombin binding.
Nagatoishi S, Isono N, Tsumoto K, Sugimoto N
Biochimie 2011 AugStepwise characterization of the thermodynamics of trichocyte intermediate filament protein supramolecular assembly.
Ishii D, Abe R, Watanabe S, Tsuchiya M, Nöcker B, Tsumoto K
J Mol Biol 2011 May 20Refolding single-chain antibody (scFv) using lauroyl-L-glutamate as a solubilization detergent and arginine as a refolding additive.
Kudou M, Ejima D, Sato H, Yumioka R, Arakawa T, Tsumoto K
Protein Expr Purif 2011 MayAn approach to rational ligand-design based on a thermodynamic analysis.
Ui M, Tsumoto K
Recent Pat Biotechnol 2010 NovStructural and energetic hot-spots for the interaction between a ladder-like polycyclic ether and the anti-ciguatoxin antibody 10C9Fab.
Ui M, Tanaka Y, Tsumuraya T, Fujii I, Inoue M, Hirama M, Tsumoto K
Mol Biosyst 2011 MarNon-core region modulates interleukin-11 signaling activity: generation of agonist and antagonist variants.
Yanaka S, Sano E, Naruse N, Miura K, Futatsumori-Sugai M, Caaveiro JM, Tsumoto K
J Biol Chem 2011 Mar 11Chemical and pharmacological chaperones: application for recombinant protein production and protein folding diseases.
Rajan RS, Tsumoto K, Tokunaga M, Tokunaga H, Kita Y, Arakawa T
Curr Med Chem 2011Salicylate restores transport function and anion exchanger activity of missense pendrin mutations.
Ishihara K, Okuyama S, Kumano S, Iida K, Hamana H, Murakoshi M, Kobayashi T, Usami S, Ikeda K, Haga Y, Tsumoto K, Nakamura H, Hirasawa N, Wada H
Hear Res 2010 Dec 1A novel protein refolding system using lauroyl-l-glutamate as a solubilizing detergent and arginine as a folding assisting agent.
Kudou M, Yumioka R, Ejima D, Arakawa T, Tsumoto K
Protein Expr Purif 2011 JanThe structural mechanism of the inhibition of archaeal RelE toxin by its cognate RelB antitoxin.
Shinohara M, Guo JX, Mori M, Nakashima T, Takagi H, Nishimoto E, Yamashita S, Tsumoto K, Kakuta Y, Kimura M
Biochem Biophys Res Commun 2010 Sep 24Expression of antibody variable region-human alkaline phosphatase fusion proteins in mammalian cells.
Sasajima Y, Iwasaki R, Tsumoto K, Kumagai I, Ihara M, Ueda H
J Immunol Methods 2010 Sep 30Gene expression profiling predicts response to temozolomide in malignant gliomas.
Yoshino A, Ogino A, Yachi K, Ohta T, Fukushima T, Watanabe T, Katayama Y, Okamoto Y, Naruse N, Sano E, Tsumoto K
Int J Oncol 2010 JunSalicylate-induced translocation of prestin having mutation in the GTSRH sequence to the plasma membrane.
Kumano S, Iida K, Ishihara K, Murakoshi M, Tsumoto K, Ikeda K, Kumagai I, Kobayashi T, Wada H
FEBS Lett 2010 Jun 3Solubilization of membrane proteins with novel N-acylamino acid detergents.
Abe R, Caaveiro JM, Kudou M, Tsumoto K
Mol Biosyst 2010 AprStep-wise refolding of recombinant proteins.
Tsumoto K, Arakawa T, Chen L
Curr Pharm Biotechnol 2010 AprNon-denaturing solubilization of inclusion bodies.
Tsumoto K, Abe R, Ejima D, Arakawa T
Curr Pharm Biotechnol 2010 AprContribution of the flexible loop region to the function of staphylococcal enterotoxin B.
Yanaka S, Kudou M, Tanaka Y, Sasaki T, Takemoto S, Sakata A, Hattori Y, Koshi T, Futaki S, Tsumoto K, Nakashima T
Protein Eng Des Sel 2010 MayReactive oxygen species induced by Streptococcus pyogenes invasion trigger apoptotic cell death in infected epithelial cells.
Aikawa C, Nozawa T, Maruyama F, Tsumoto K, Hamada S, Nakagawa I
Cell Microbiol 2010 JunTemperature and salt concentration alter base-sequence selectivity of a duplex DNA-binding protein.
Nagatoishi S, Tanaka Y, Kudou M, Tsumoto K
Mol Biosyst 2010 JanContribution of asparagine residues to the stabilization of a proteinaceous antigen-antibody complex, HyHEL-10-hen egg white lysozyme.
Yokota A, Tsumoto K, Shiroishi M, Nakanishi T, Kondo H, Kumagai I
J Biol Chem 2010 Mar 5Direct and selective immobilization of proteins by means of an inorganic material-binding peptide: discussion on functionalization in the elongation to material-binding peptide.
Yokoo N, Togashi T, Umetsu M, Tsumoto K, Hattori T, Nakanishi T, Ohara S, Takami S, Naka T, Abe H, Kumagai I, Adschiri T
J Phys Chem B 2010 Jan 14Signal peptide design for improving recombinant protein secretion in the baculovirus expression vector system.
Futatsumori-Sugai M, Tsumoto K
Biochem Biophys Res Commun 2010 Jan 1MEP HyperCel chromatography II: binding, washing and elution.
Arakawa T, Futatsumori-Sugai M, Tsumoto K, Kita Y, Sato H, Ejima D
Protein Expr Purif 2010 Jun[Rational fragment-design method based on a thermodynamic analysis].
Tsumoto K, Ui M
Yakugaku Zasshi 2009 NovScreening of effective column rinse solvent for Protein-A chromatography.
Yumioka R, Tsumoto K, Arakawa T, Ejima D
Protein Expr Purif 2010 AprObservation of the membrane binding activity and domain structure of gpV, which comprises the tail spike of bacteriophage P2.
Kageyama Y, Murayama M, Onodera T, Yamada S, Fukada H, Kudou M, Tsumoto K, Toyama Y, Kado S, Kubota K, Takeda S
Biochemistry 2009 Oct 27The interaction of hyperthermophilic TATA-box binding protein with single-stranded DNA is entropically favorable and exhibits a large negative heat capacity change at high salt concentration.
Nagatoishi S, Tanaka Y, Kudou M, Tsumoto K
Mol Biosyst 2009 Sep[Recognition mechanism for poly-cyclic ether toxin by anti-ciguatoxin antibody].
Ui M, Tsumoto K
Tanpakushitsu Kakusan Koso 2009 JulTo be excluded or to bind, that is the question: Arginine effects on proteins.
Nakakido M, Kudou M, Arakawa T, Tsumoto K
Curr Pharm Biotechnol 2009 JunContributions of interfacial residues of human Interleukin15 to the specificity and affinity for its private alpha-receptor.
Sakamoto S, Caaveiro JM, Sano E, Tanaka Y, Kudou M, Tsumoto K
J Mol Biol 2009 Jun 26Impact of intrinsic cooperative thermodynamics of peptide-MHC complexes on antiviral activity of HIV-specific CTL.
Motozono C, Yanaka S, Tsumoto K, Takiguchi M, Ueno T
J Immunol 2009 May 1Utilization of Arg-elution method for FLAG-tag based chromatography.
Futatsumori-Sugai M, Abe R, Watanabe M, Kudou M, Yamamoto T, Ejima D, Arakawa T, Tsumoto K
Protein Expr Purif 2009 OctImmobilized metal affinity chromatography in the presence of arginine.
Abe R, Kudou M, Tanaka Y, Arakawa T, Tsumoto K
Biochem Biophys Res Commun 2009 Apr 10Molecular glues carrying multiple guanidinium ion pendants via an oligoether spacer: stabilization of microtubules against depolymerization.
Okuro K, Kinbara K, Tsumoto K, Ishii N, Aida T
J Am Chem Soc 2009 Feb 11Activation of leukemia-associated RhoGEF by Galpha13 with significant conformational rearrangements in the interface.
Suzuki N, Tsumoto K, Hajicek N, Daigo K, Tokita R, Minami S, Kodama T, Hamakubo T, Kozasa T
J Biol Chem 2009 Feb 20Growth control of hybridoma cells with an artificially induced EpoR-gp130 heterodimer.
Kawahara M, Ogo Y, Tsumoto K, Kumagai I, Ueda H, Nagamune T
Cytotechnology 2006 NovElectron microscopy and computational studies of Ebh, a giant cell-wall-associated protein from Staphylococcus aureus.
Sakamoto S, Tanaka Y, Tanaka I, Takei T, Yu J, Kuroda M, Yao M, Ohta T, Tsumoto K
Biochem Biophys Res Commun 2008 Nov 14Structure-based analysis reveals hydration changes induced by arginine hydrochloride.
Nakakido M, Tanaka Y, Mitsuhori M, Kudou M, Ejima D, Arakawa T, Tsumoto K
Biophys Chem 2008 OctStructural basis for multimeric heme complexation through a specific protein-heme interaction: the case of the third neat domain of IsdH from Staphylococcus aureus.
Watanabe M, Tanaka Y, Suenaga A, Kuroda M, Yao M, Watanabe N, Arisaka F, Ohta T, Tanaka I, Tsumoto K
J Biol Chem 2008 Oct 17Staphylococcus aureus giant protein Ebh is involved in tolerance to transient hyperosmotic pressure.
Kuroda M, Tanaka Y, Aoki R, Shu D, Tsumoto K, Ohta T
Biochem Biophys Res Commun 2008 Sep 19Selective expansion of genetically modified T cells using an antibody/interleukin-2 receptor chimera.
Sogo T, Kawahara M, Tsumoto K, Kumagai I, Ueda H, Nagamune T
J Immunol Methods 2008 Aug 20Expression, purification, crystallization and preliminary diffraction analysis of CapF, a capsular polysaccharide-synthesis enzyme from Staphylococcus aureus.
Miyafusa T, Tanaka Y, Kuroda M, Ohta T, Tsumoto K
Acta Crystallogr Sect F Struct Biol Cryst Commun 2008 Jun 1How protein recognizes ladder-like polycyclic ethers. Interactions between ciguatoxin (CTX3C) fragments and its specific antibody 10C9.
Ui M, Tanaka Y, Tsumuraya T, Fujii I, Inoue M, Hirama M, Tsumoto K
J Biol Chem 2008 Jul 11Thermodynamic analysis reveals that GTP binding affects the interaction between the alpha- and gamma-subunits of translation initiation factor 2.
Nakakido M, Tanaka Y, Sokabe M, Tsumoto K
Biochem Biophys Res Commun 2008 Jul 11A helical string of alternately connected three-helix bundles for the cell wall-associated adhesion protein Ebh from Staphylococcus aureus.
Tanaka Y, Sakamoto S, Kuroda M, Goda S, Gao YG, Tsumoto K, Hiragi Y, Yao M, Watanabe N, Ohta T, Tanaka I
Structure 2008 MarCritical contribution of aromatic rings to specific recognition of polyether rings. The case of ciguatoxin CTX3C-ABC and its specific antibody 1C49.
Tsumoto K, Yokota A, Tanaka Y, Ui M, Tsumuraya T, Fujii I, Kumagai I, Nagumo Y, Oguri H, Inoue M, Hirama M
J Biol Chem 2008 May 2[Solvent modulation of chromatography].
Arakawa T, Ejima D, Tsumoto K, Gagnon P
Seikagaku 2008 JanEffect of an amyloidogenic sequence attached to yellow fluorescent protein.
Hamada D, Tsumoto K, Sawara M, Tanaka N, Nakahira K, Shiraki K, Yanagihara I
Proteins 2008 Aug 15Critical contribution of VH-VL interaction to reshaping of an antibody: the case of humanization of anti-lysozyme antibody, HyHEL-10.
Nakanishi T, Tsumoto K, Yokota A, Kondo H, Kumagai I
Protein Sci 2008 FebGrafting of material-binding function into antibodies Functionalization by peptide grafting.
Hattori T, Umetsu M, Nakanishi T, Tsumoto K, Ohara S, Abe H, Naito M, Asano R, Adschiri T, Kumagai I
Biochem Biophys Res Commun 2008 Jan 25Thermodynamic consequences of mutations in vernier zone residues of a humanized anti-human epidermal growth factor receptor murine antibody, 528.
Makabe K, Nakanishi T, Tsumoto K, Tanaka Y, Kondo H, Umetsu M, Sone Y, Asano R, Kumagai I
J Biol Chem 2008 Jan 11Human CD4+ central and effector memory T cells produce IL-21: effect on cytokine-driven proliferation of CD4+ T cell subsets.
Onoda T, Rahman M, Nara H, Araki A, Makabe K, Tsumoto K, Kumagai I, Kudo T, Ishii N, Tanaka N, Sugamura K, Hayasaka K, Asao H
Int Immunol 2007 OctCrystal structure analysis reveals a novel forkhead-associated domain of ESAT-6 secretion system C protein in Staphylococcus aureus.
Tanaka Y, Kuroda M, Yasutake Y, Yao M, Tsumoto K, Watanabe N, Ohta T, Tanaka I
Proteins 2007 Nov 15The N-terminal domain of elastin-binding protein of Staphylococcus aureus changes its secondary structure in a membrane-mimetic environment.
Nakakido M, Tanaka Y, Tsumoto K
J Biochem 2007 AugMolecular properties of two proteins homologous to PduO-type ATP:cob(I)alamin adenosyltransferase from Sulfolobus tokodaii.
Tanaka Y, Sasaki T, Kumagai I, Yasutake Y, Yao M, Tanaka I, Tsumoto K
Proteins 2007 Aug 1Arginine improves protein elution in hydrophobic interaction chromatography. The cases of human interleukin-6 and activin-A.
Tsumoto K, Ejima D, Nagase K, Arakawa T
J Chromatogr A 2007 Jun 22Mimicry of erythropoietin and interleukin-6 signalling by an antibody/cytokine receptor chimera in murine myeloid 32D cells.
Kawahara M, Ueda H, Tsumoto K, Kumagai I, Nagamune T
J Biochem 2007 AprThe effects of arginine on protein binding and elution in hydrophobic interaction and ion-exchange chromatography.
Arakawa T, Tsumoto K, Nagase K, Ejima D
Protein Expr Purif 2007 JulA human antibody fragment with high affinity for biodegradable polymer film.
Watanabe H, Tsumoto K, Taguchi S, Yamashita K, Doi Y, Nishimiya Y, Kondo H, Umetsu M, Kumagai I
Bioconjug Chem 2007 May-JunSuppression of protein interactions by arginine: a proposed mechanism of the arginine effects.
Arakawa T, Ejima D, Tsumoto K, Obeyama N, Tanaka Y, Kita Y, Timasheff SN
Biophys Chem 2007 AprEffects of salts on protein-surface interactions: applications for column chromatography.
Tsumoto K, Ejima D, Senczuk AM, Kita Y, Arakawa T
J Pharm Sci 2007 JulInduced binding of proteins by ammonium sulfate in affinity and ion-exchange column chromatography.
Arakawa T, Tsumoto K, Ejima D, Kita Y, Yonezawa Y, Tokunaga M
J Biochem Biophys Methods 2007 Apr 10Structural and mutational analyses of Drp35 from Staphylococcus aureus: a possible mechanism for its lactonase activity.
Tanaka Y, Morikawa K, Ohki Y, Yao M, Tsumoto K, Watanabe N, Ohta T, Tanaka I
J Biol Chem 2007 Feb 23Structural consequences of mutations in interfacial Tyr residues of a protein antigen-antibody complex. The case of HyHEL-10-HEL.
Shiroishi M, Tsumoto K, Tanaka Y, Yokota A, Nakanishi T, Kondo H, Kumagai I
J Biol Chem 2007 Mar 2Effects of acid exposure on the conformation, stability, and aggregation of monoclonal antibodies.
Ejima D, Tsumoto K, Fukada H, Yumioka R, Nagase K, Arakawa T, Philo JS
Proteins 2007 Mar 1Circular dichroism spectra demonstrate formation of the thrombin-binding DNA aptamer G-quadruplex under stabilizing-cation-deficient conditions.
Nagatoishi S, Tanaka Y, Tsumoto K
Biochem Biophys Res Commun 2007 Jan 19Improved performance of column chromatography by arginine: dye-affinity chromatography.
Arakawa T, Ejima D, Tsumoto K, Ishibashi M, Tokunaga M
Protein Expr Purif 2007 AprAggregation suppression of proteins by arginine during thermal unfolding.
Arakawa T, Kita Y, Ejima D, Tsumoto K, Fukada H
Protein Pept Lett 2006Structural basis for recognition of the nonclassical MHC molecule HLA-G by the leukocyte Ig-like receptor B2 (LILRB2/LIR2/ILT4/CD85d).
Shiroishi M, Kuroki K, Rasubala L, Tsumoto K, Kumagai I, Kurimoto E, Kato K, Kohda D, Maenaka K
Proc Natl Acad Sci U S A 2006 Oct 31Small molecule pharmacological chaperones: From thermodynamic stabilization to pharmaceutical drugs.
Arakawa T, Ejima D, Kita Y, Tsumoto K
Biochim Biophys Acta 2006 NovHumanization of the bispecific epidermal growth factor receptor x CD3 diabody and its efficacy as a potential clinical reagent.
Asano R, Sone Y, Makabe K, Tsumoto K, Hayashi H, Katayose Y, Unno M, Kudo T, Kumagai I
Clin Cancer Res 2006 Jul 1Imaging by atomic force microscopy of the plasma membrane of prestin-transfected Chinese hamster ovary cells.
Murakoshi M, Gomi T, Iida K, Kumano S, Tsumoto K, Kumagai I, Ikeda K, Kobayashi T, Wada H
J Assoc Res Otolaryngol 2006 Sep[Dissection of specificity and affinity of an antibody for its specific antigen].
Tsumoto K
Seikagaku 2006 FebCharacterization of arginine as a solvent additive: a halophilic enzyme as a model protein.
Ishibashi M, Tsumoto K, Ejima D, Arakawa T, Tokunaga M
Protein Pept Lett 2005 OctReview: Why is arginine effective in suppressing aggregation?
Tsumoto K, Ejima D, Kita Y, Arakawa T
Protein Pept Lett 2005 OctEfficient leukocyte Ig-like receptor signaling and crystal structure of disulfide-linked HLA-G dimer.
Shiroishi M, Kuroki K, Ose T, Rasubala L, Shiratori I, Arase H, Tsumoto K, Kumagai I, Kohda D, Maenaka K
J Biol Chem 2006 Apr 14Entropically driven MHC class I recognition by human inhibitory receptor leukocyte Ig-like receptor B1 (LILRB1/ILT2/CD85j).
Shiroishi M, Kuroki K, Tsumoto K, Yokota A, Sasaki T, Amano K, Shimojima T, Shirakihara Y, Rasubala L, van der Merwe PA, Kumagai I, Kohda D, Maenaka K
J Mol Biol 2006 Jan 13Arginine as an effective additive in gel permeation chromatography.
Ejima D, Yumioka R, Arakawa T, Tsumoto K
J Chromatogr A 2005 Nov 11Selection of highly productive mammalian cells based on an inducible growth advantage using an antibody/receptor chimera.
Kawahara M, Ueda H, Tsumoto K, Kumagai I, Mahoney W, Nagamune T
J Biosci Bioeng 2002A novel "reverse screening" to identify refolding additives for activin-A.
Ejima D, Ono K, Tsumoto K, Arakawa T, Eto Y
Protein Expr Purif 2006 MayCrystal structure of the hypothetical protein ST2072 from Sulfolobus tokodaii.
Tanaka Y, Tsumoto K, Tanabe E, Yasutake Y, Sakai N, Yao M, Tanaka I, Kumagai I
Proteins 2005 Dec 1Effective elution of antibodies by arginine and arginine derivatives in affinity column chromatography.
Ejima D, Yumioka R, Tsumoto K, Arakawa T
Anal Biochem 2005 Oct 15Construction of an expression system for the motor protein prestin in Chinese hamster ovary cells.
Iida K, Tsumoto K, Ikeda K, Kumagai I, Kobayashi T, Wada H
Hear Res 2005 JulKinetics of ultrasonic disinfection of Escherichia coli in the presence of titanium dioxide particles.
Kubo M, Onodera R, Shibasaki-Kitakawa N, Tsumoto K, Yonemoto T
Biotechnol Prog 2005 May-JunIs arginine a protein-denaturant?
Ishibashi M, Tsumoto K, Tokunaga M, Ejima D, Kita Y, Arakawa T
Protein Expr Purif 2005 JulHerpes simplex virus targeting to the EGF receptor by a gD-specific soluble bridging molecule.
Nakano K, Asano R, Tsumoto K, Kwon H, Goins WF, Kumagai I, Cohen JB, Glorioso JC
Mol Ther 2005 AprNondenaturing solubilization of beta2 microglobulin from inclusion bodies by L-arginine.
Umetsu M, Tsumoto K, Nitta S, Adschiri T, Ejima D, Arakawa T, Kumagai I
Biochem Biophys Res Commun 2005 Mar 4Tumor-directed lymphocyte-activating cytokines: refolding-based preparation of recombinant human interleukin-12 and an antibody variable domain-fused protein by additive-introduced stepwise dialysis.
Makabe K, Asano R, Ito T, Tsumoto K, Kudo T, Kumagai I
Biochem Biophys Res Commun 2005 Mar 4Role of arginine in protein refolding, solubilization, and purification.
Tsumoto K, Umetsu M, Kumagai I, Ejima D, Philo JS, Arakawa T
Biotechnol Prog 2004 Sep-OctStructural evidence for guanidine-protein side chain interactions: crystal structure of CutA from Pyrococcus horikoshii in 3 M guanidine hydrochloride.
Tanaka Y, Tsumoto K, Umetsu M, Nakanishi T, Yasutake Y, Sakai N, Yao M, Tanaka I, Arakawa T, Kumagai I
Biochem Biophys Res Commun 2004 Oct 8Phage-display selection of antibodies to the left end of CTX3C using synthetic fragments.
Nagumo Y, Oguri H, Tsumoto K, Shindo Y, Hirama M, Tsumuraya T, Fujii I, Tomioka Y, Mizugaki M, Kumagai I
J Immunol Methods 2004 JunElution of antibodies from a Protein-A column by aqueous arginine solutions.
Arakawa T, Philo JS, Tsumoto K, Yumioka R, Ejima D
Protein Expr Purif 2004 AugReversal of antigen-dependent signaling by two mutations in antibody/receptor chimera: implication of inverse agonism in cytokine receptor superfamily.
Kawahara M, Ishii S, Tsumoto K, Kumagai I, Ueda H, Nagamune T
Biochem Pharmacol 2004 Aug 1How oligomerization contributes to the thermostability of an archaeon protein. Protein L-isoaspartyl-O-methyltransferase from Sulfolobus tokodaii.
Tanaka Y, Tsumoto K, Yasutake Y, Umetsu M, Yao M, Fukada H, Tanaka I, Kumagai I
J Biol Chem 2004 Jul 30Fucose depletion from human IgG1 oligosaccharide enhances binding enthalpy and association rate between IgG1 and FcgammaRIIIa.
Okazaki A, Shoji-Hosaka E, Nakamura K, Wakitani M, Uchida K, Kakita S, Tsumoto K, Kumagai I, Shitara K
J Mol Biol 2004 Mar 5Engineering amyloidogenicity towards the development of nanofibrillar materials.
Hamada D, Yanagihara I, Tsumoto K
Trends Biotechnol 2004 FebStructural characteristics and refolding of in vivo aggregated hyperthermophilic archaeon proteins.
Umetsu M, Tsumoto K, Ashish K, Nitta S, Tanaka Y, Adschiri T, Kumagai I
FEBS Lett 2004 Jan 16AMEGA: antigen-mediated genetically modified cell amplification.
Kawahara M, Ueda H, Tsumoto K, Kumagai I, Nagamune T
J Immunol Methods 2004 JanStructural implications for heavy metal-induced reversible assembly and aggregation of a protein: the case of Pyrococcus horikoshii CutA.
Tanaka Y, Tsumoto K, Nakanishi T, Yasutake Y, Sakai N, Yao M, Tanaka I, Kumagai I
FEBS Lett 2004 Jan 2[A magic agent in protein refolding: arginine].
Arakawa T, Katsuyama I, Kumagai I, Tsumoto K
Tanpakushitsu Kakusan Koso 2003 DecSolubilization of active green fluorescent protein from insoluble particles by guanidine and arginine.
Tsumoto K, Umetsu M, Kumagai I, Ejima D, Arakawa T
Biochem Biophys Res Commun 2003 Dec 26A highly effective and stable bispecific diabody for cancer immunotherapy: cure of xenografted tumors by bispecific diabody and T-LAK cells.
Hayashi H, Asano R, Tsumoto K, Katayose Y, Suzuki M, Unno M, Kodama H, Takemura S, Yoshida H, Makabe K, Imai K, Matsuno S, Kumagai I, Kudo T
Cancer Immunol Immunother 2004 JunAn optimized homogeneous noncompetitive immunoassay based on the antigen-driven enzymatic complementation.
Ueda H, Yokozeki T, Arai R, Tsumoto K, Kumagai I, Nagamune T
J Immunol Methods 2003 AugOn-column refolding and characterization of soluble human interleukin-15 receptor alpha-chain produced in Escherichia coli.
Matsumoto M, Misawa S, Tsumoto K, Kumagai I, Hayashi H, Kobayashi Y
Protein Expr Purif 2003 SepImmobilized oxidoreductase as an additive for refolding inclusion bodies: application to antibody fragments.
Tsumoto K, Umetsu M, Yamada H, Ito T, Misawa S, Kumagai I
Protein Eng 2003 JulHuman inhibitory receptors Ig-like transcript 2 (ILT2) and ILT4 compete with CD8 for MHC class I binding and bind preferentially to HLA-G.
Shiroishi M, Tsumoto K, Amano K, Shirakihara Y, Colonna M, Braud VM, Allan DS, Makadzange A, Rowland-Jones S, Willcox B, Jones EY, van der Merwe PA, Kumagai I, Maenaka K
Proc Natl Acad Sci U S A 2003 Jul 22Structural consequences of target epitope-directed functional alteration of an antibody. The case of anti-hen lysozyme antibody, HyHEL-10.
Kumagai I, Nishimiya Y, Kondo H, Tsumoto K
J Biol Chem 2003 Jul 4The effects of arginine on refolding of aggregated proteins: not facilitate refolding, but suppress aggregation.
Arakawa T, Tsumoto K
Biochem Biophys Res Commun 2003 Apr 25Bypassing antibiotic selection: positive screening of genetically modified cells with an antigen-dependent proliferation switch.
Kawahara M, Ueda H, Morita S, Tsumoto K, Kumagai I, Nagamune T
Nucleic Acids Res 2003 Apr 1Practical considerations in refolding proteins from inclusion bodies.
Tsumoto K, Ejima D, Kumagai I, Arakawa T
Protein Expr Purif 2003 MarA morphologic study of filamentous phage infection of Escherichia coli using biotinylated phages.
Nakamura M, Tsumoto K, Kumagai I, Ishimura K
FEBS Lett 2003 Feb 11How additives influence the refolding of immunoglobulin-folded proteins in a stepwise dialysis system. Spectroscopic evidence for highly efficient refolding of a single-chain Fv fragment.
Umetsu M, Tsumoto K, Hara M, Ashish K, Goda S, Adschiri T, Kumagai I
J Biol Chem 2003 Mar 14Efficient construction of a diabody using a refolding system: anti-carcinoembryonic antigen recombinant antibody fragment.
Asano R, Kudo T, Nishimura Y, Makabe K, Hayashi H, Suzuki M, Tsumoto K, Kumagai I
J Biochem 2002 DecThe role of hydrogen bonding via interfacial water molecules in antigen-antibody complexation. The HyHEL-10-HEL interaction.
Yokota A, Tsumoto K, Shiroishi M, Kondo H, Kumagai I
J Biol Chem 2003 Feb 14Antitumor activity of interleukin-21 prepared by novel refolding procedure from inclusion bodies expressed in Escherichia coli.
Asano R, Kudo T, Makabe K, Tsumoto K, Kumagai I
FEBS Lett 2002 Sep 25Inhibition of hepatitis C virus NS3 protease by peptides derived from complementarity-determining regions (CDRs) of the monoclonal antibody 8D4: tolerance of a CDR peptide to conformational changes of a target.
Tsumoto K, Misawa S, Ohba Y, Ueno T, Hayashi H, Kasai N, Watanabe H, Asano R, Kumagai I
FEBS Lett 2002 Aug 14Chiral recognition in the binding of helicenediamine to double strand DNA: interactions between low molecular weight helical compounds and a helical polymer.
Honzawa S, Okubo H, Anzai S, Yamaguchi M, Tsumoto K, Kumagai I
Bioorg Med Chem 2002 OctSelection of human antibody fragments on the basis of stabilization of the variable domain in the presence of target antigens.
Watanabe H, Tsumoto K, Asano R, Nishimiya Y, Kumagai I
Biochem Biophys Res Commun 2002 Jul 5The effect of an agglutogen on virus infection: biotinylated filamentous phages and avidin as a model.
Nakamura M, Tsumoto K, Ishimura K, Kumagai I
FEBS Lett 2002 Jun 5T-cell immunotherapy for human MK-1-expressing tumors using a fusion protein of the superantigen SEA and anti-MK-1 scFv antibody.
Ueno A, Arakawa F, Abe H, Matsumoto H, Kudo T, Asano R, Tsumoto K, Kumagai I, Kuroki M, Kuroki M
Anticancer Res 2002 Mar-AprDetection of biotinylated proteins in polyacrylamide gels using an avidin-fluorescein conjugate.
Nakamura M, Tsumoto K, Ishimura K, Kumagai I
Anal Biochem 2002 May 15Phage library panning against cytosolic fraction of cells using quantitative dot blotting assay: application of selected VH to histochemistry.
Nakamura M, Tsumoto K, Ishimura K, Kumagai I
J Immunol Methods 2002 Mar 1A mutated superantigen SEA D227A fusion diabody specific to MUC1 and CD3 in targeted cancer immunotherapy for bile duct carcinoma.
Takemura S, Kudo T, Asano R, Suzuki M, Tsumoto K, Sakurai N, Katayose Y, Kodama H, Yoshida H, Ebara S, Saeki H, Imai K, Matsuno S, Kumagai I
Cancer Immunol Immunother 2002 MarMutated SEA-D227A-conjugated antibodies greatly enhance antitumor activity against MUC1-expressing bile duct carcinoma.
Kodama H, Suzuki M, Katayose Y, Shinoda M, Sakurai N, Takemura S, Yoshida H, Saeki H, Ichiyama M, Tsumoto K, Asano R, Kumagai I, Imai K, Hinoda Y, Matsuno S, Kudo T
Cancer Immunol Immunother 2001 DecA visualization method of filamentous phage infection and phage-derived proteins in Escherichia coli using biotinylated phages.
Nakamura M, Tsumoto K, Ishimura K, Kumagai I
Biochem Biophys Res Commun 2001 Nov 23Contribution of Thr29 to the thermodynamic stability of goat alpha-lactalbumin as determined by experimental and theoretical approaches.
Horii K, Saito M, Yoda T, Tsumoto K, Matsushima M, Kuwajima K, Kumagai I
Proteins 2001 Oct 1[Refolding of proteins from inclusion bodies].
Tsumoto K, Kumagai I, Misawa S
Tanpakushitsu Kakusan Koso 2001 JulA growth signal with an artificially induced erythropoietin receptor-gp130 cytoplasmic domain heterodimer.
Kawahara M, Ueda H, Tsumoto K, Kumagai I, Mahoney W, Nagamune T
J Biochem 2001 AugReplacing factor-dependency with that for lysozyme: affordable culture of IL-6-dependent hybridoma by transfecting artificial cell surface receptor.
Kawahara M, Natsume A, Terada S, Kato K, Tsumoto K, Kumagai I, Miki M, Mahoney W, Ueda H, Nagamune T
Biotechnol Bioeng 2001 Sep 5Structural evidence for entropic contribution of salt bridge formation to a protein antigen-antibody interaction: the case of hen lysozyme-HyHEL-10 Fv complex.
Shiroishi M, Yokota A, Tsumoto K, Kondo H, Nishimiya Y, Horii K, Matsushima M, Ogasahara K, Yutani K, Kumagai I
J Biol Chem 2001 Jun 22Inhibition of the hepatitis C virus NS3 protease activity by Fv fragment of antibody 8D4.
Kasai N, Tsumoto K, Niwa S, Misawa S, Ueno T, Hayashi H, Kumagai I
Biochem Biophys Res Commun 2001 Feb 23Panning of a phage VH library using nitrocellulose membranes: application to selection of a human VH library.
Nakamura M, Watanabe H, Nishimiya Y, Tsumoto K, Ishimura K, Kumagai I
J Biochem 2001 FebDemonstration of a homogeneous noncompetitive immunoassay based on bioluminescence resonance energy transfer.
Arai R, Nakagawa H, Tsumoto K, Mahoney W, Kumagai I, Ueda H, Nagamune T
Anal Biochem 2001 Feb 1Folding-unfolding of goat alpha-lactalbumin studied by stopped-flow circular dichroism and molecular dynamics simulations.
Yoda T, Saito M, Arai M, Horii K, Tsumoto K, Matsushima M, Kumagai I, Kuwajima K
Proteins 2001 Jan 1Construction of a diabody (small recombinant bispecific antibody) using a refolding system.
Takemura S, Asano R, Tsumoto K, Ebara S, Sakurai N, Katayose Y, Kodama H, Yoshida H, Suzuki M, Imai K, Matsuno S, Kudo T, Kumagai I
Protein Eng 2000 AugCell-growth control by monomeric antigen: the cell surface expression of lysozyme-specific Ig V-domains fused to truncated Epo receptor.
Ueda H, Kawahara M, Aburatani T, Tsumoto K, Todokoro K, Suzuki E, Nishimura H, Schueler PA, Winter G, Mahoney WC, Kumagai I, Nagamune T
J Immunol Methods 2000 Jul 31Functional Fv fragment of an antibody specific for CD28: Fv-mediated co-stimulation of T cells.
Takemura S, Asano R, Tsumoto K, Arai T, Sakurai N, Kodama H, Yoshida H, Katayose Y, Suzuki M, Matsuno S, Kudo T, Kumagai I
FEBS Lett 2000 Jul 7Isolation and characterization of monoclonal antibodies that inhibit hepatitis C virus NS3 protease.
Ueno T, Misawa S, Ohba Y, Matsumoto M, Mizunuma M, Kasai N, Tsumoto K, Kumagai I, Hayashi H
J Virol 2000 JulFluorolabeling of antibody variable domains with green fluorescent protein variants: application to an energy transfer-based homogeneous immunoassay.
Arai R, Ueda H, Tsumoto K, Mahoney WC, Kumagai I, Nagamune T
Protein Eng 2000 MayThermodynamic consequences of grafting enhanced affinity toward the mutated antigen onto an antibody. The case of anti-lysozyme antibody, HyHEL-10.
Nishimiya Y, Tsumoto K, Shiroishi M, Yutani K, Kumagai I
J Biol Chem 2000 Apr 28Functional construction of the anti-mucin core protein (MUC1) antibody MUSE11 variable regions in a bacterial expression system.
Asano R, Takemura S, Tsumoto K, Sakurai N, Teramae A, Ebara S, Katayose Y, Shinoda M, Suzuki M, Imai K, Matsuno S, Kudo T, Kumagai I
J Biochem 2000 AprHomogeneous noncompetitive immunoassay based on the energy transfer between fluorolabeled antibody variable domains (open sandwich fluoroimmunoassay).
Ueda H, Kubota K, Wang Y, Tsumoto K, Mahoney W, Kumagai I, Nagamune T
Biotechniques 1999 OctCrystal structure of anti-Hen egg white lysozyme antibody (HyHEL-10) Fv-antigen complex. Local structural changes in the protein antigen and water-mediated interactions of Fv-antigen and light chain-heavy chain interfaces.
Kondo H, Shiroishi M, Matsushima M, Tsumoto K, Kumagai I
J Biol Chem 1999 Sep 24[Antibody production by phage library].
Kumagai I, Tsumoto K
Nihon Seirigaku Zasshi 1999Open sandwich ELISA with V(H)-/V(L)-alkaline phosphatase fusion proteins.
Suzuki C, Ueda H, Tsumoto K, Mahoney WC, Kumagai I, Nagamune T
J Immunol Methods 1999 Apr 22SEA-scFv as a bifunctional antibody: construction of a bacterial expression system and its functional analysis.
Sakurai N, Kudo T, Suzuki M, Tsumoto K, Takemura S, Kodama H, Ebara S, Teramae A, Katayose Y, Shinoda M, Kurokawa T, Hinoda Y, Imai K, Matsuno S, Kumagai I
Biochem Biophys Res Commun 1999 Mar 5Cell-free expression of two single-chain monoclonal antibodies against lysozyme: effect of domain arrangement on the expression.
Merk H, Stiege W, Tsumoto K, Kumagai I, Erdmann VA
J Biochem 1999 FebEffect of the extra n-terminal methionine residue on the stability and folding of recombinant alpha-lactalbumin expressed in Escherichia coli.
Chaudhuri TK, Horii K, Yoda T, Arai M, Nagata S, Terada TP, Uchiyama H, Ikura T, Tsumoto K, Kataoka H, Matsushima M, Kuwajima K, Kumagai I
J Mol Biol 1999 Jan 22Highly efficient recovery of functional single-chain Fv fragments from inclusion bodies overexpressed in Escherichia coli by controlled introduction of oxidizing reagent--application to a human single-chain Fv fragment.
Tsumoto K, Shinoki K, Kondo H, Uchikawa M, Juji T, Kumagai I
J Immunol Methods 1998 Oct 1Calorimetric study of mutant human lysozymes with partially introduced Ca2+ binding sites and its efficient refolding system from inclusion bodies.
Koshiba T, Tsumoto K, Masaki K, Kawano K, Nitta K, Kumagai I
Protein Eng 1998 AugConstruction of mono- and bivalent human single-chain Fv fragments against the D antigen in the Rh blood group: multimerization effect on cell agglutination and application to blood typing.
Furuta M, Uchikawa M, Ueda Y, Yabe T, Taima T, Tsumoto K, Kojima S, Juji T, Kumagai I
Protein Eng 1998 MarNovel selection method for engineered antibodies using the mechanism of Fv fragment stabilization in the presence of antigen.
Tsumoto K, Nishimiya Y, Kasai N, Ueda H, Nagamune T, Ogasahara K, Yutani K, Tokuhisa K, Matsushima M, Kumagai I
Protein Eng 1997 Nov[Generation of novel functional antibody molecules by in vitro selection system].
Kumagai I, Tsumoto K
Tanpakushitsu Kakusan Koso 1998 FebRole of salt bridge formation in antigen-antibody interaction. Entropic contribution to the complex between hen egg white lysozyme and its monoclonal antibody HyHEL10.
Tsumoto K, Ogasahara K, Ueda Y, Watanabe K, Yutani K, Kumagai I
J Biol Chem 1996 Dec 20Open sandwich ELISA: a novel immunoassay based on the interchain interaction of antibody variable region.
Ueda H, Tsumoto K, Kubota K, Suzuki E, Nagamune T, Nishimura H, Schueler PA, Winter G, Kumagai I, Mohoney WC
Nat Biotechnol 1996 DecA stable phage-display system using a phagemid vector: phage display of hen egg-white lysozyme (HEL), Escherichia coli alkaline, phosphatase, and anti-HEL monoclonal antibody, HyHEL10.
Maenaka K, Furuta M, Tsumoto K, Watanabe K, Ueda Y, Kumagai I
Biochem Biophys Res Commun 1996 Jan 26Role of Tyr residues in the contact region of anti-lysozyme monoclonal antibody HyHEL10 for antigen binding.
Tsumoto K, Ogasahara K, Ueda Y, Watanabe K, Yutani K, Kumagai I
J Biol Chem 1995 Aug 4Contribution to antibody-antigen interaction of structurally perturbed antigenic residues upon antibody binding.
Tsumoto K, Ueda Y, Maenaka K, Watanabe K, Ogasahara K, Yutani K, Kumagai I
J Biol Chem 1994 Nov 18Effect of the order of antibody variable regions on the expression of the single-chain HyHEL10 Fv fragment in E. coli and the thermodynamic analysis of its antigen-binding properties.
Tsumoto K, Nakaoki Y, Ueda Y, Ogasahara K, Yutani K, Watanabe K, Kumagai I
Biochem Biophys Res Commun 1994 Jun 15Synthesis and expression of a DNA encoding the Fv domain of an anti-lysozyme monoclonal antibody, HyHEL10, in Streptomyces lividans.
Ueda Y, Tsumoto K, Watanabe K, Kumagai I
Gene 1993 Jul 15A protease inhibitor produced by Streptomyces lividans 66 exhibits inhibitory activities toward both subtilisin BPN' and trypsin.
Ueda Y, Kojima S, Tsumoto K, Takeda S, Miura K, Kumagai I
J Biochem 1992 Aug