An epitope-directed antibody affinity maturation system utilizing mammalian cell survival as readout.

Eguchi A, Nakakido M, Nagatoishi S, Kuroda D, Tsumoto K, Nagamune T, Kawahara M

Exploring designability of electrostatic complementarity at an antigen-antibody interface directed by mutagenesis, biophysical analysis, and molecular dynamics simulations.

Yoshida K, Kuroda D, Kiyoshi M, Nakakido M, Nagatoishi S, Soga S, Shirai H, Tsumoto K

Design strategy for serine hydroxymethyltransferase probes based on retro-aldol-type reaction.

Nonaka H, Nakanishi Y, Kuno S, Ota T, Mochidome K, Saito Y, Sugihara F, Takakusagi Y, Aoki I, Nagatoishi S, Tsumoto K, Sando S

Control of protein adsorption to cyclo olefin polymer by the Hofmeister effect.

Fujita R, Nagatoishi S, Adachi S, Nishioka H, Ninomiya H, Kaya T, Takai M, Arakawa T, Tsumoto K

Phospholipid Membrane Fluidity Alters Ligand Binding Activity of a G Protein-Coupled Receptor by Shifting the Conformational Equilibrium.

Yoshida K, Nagatoishi S, Kuroda D, Suzuki N, Murata T, Tsumoto K

Generation and characterization of antagonistic anti-human interleukin (IL)-18 monoclonal antibodies with high affinity: Two types of monoclonal antibodies against full-length IL-18 and the neoepitope of inflammatory caspase-cleaved active IL-18.

Nariai Y, Kamino H, Obayashi E, Kato H, Sakashita G, Sugiura T, Migita K, Koga T, Kawakami A, Sakamoto K, Kadomatsu K, Nakakido M, Tsumoto K, Urano T

Affinity Improvement of a Cancer-Targeted Antibody through Alanine-Induced Adjustment of Antigen-Antibody Interface.

Yamashita T, Mizohata E, Nagatoishi S, Watanabe T, Nakakido M, Iwanari H, Mochizuki Y, Nakayama T, Kado Y, Yokota Y, Matsumura H, Kawamura T, Kodama T, Hamakubo T, Inoue T, Fujitani H, Tsumoto K

Thermodynamic and computational analyses reveal the functional roles of the galloyl group of tea catechins in molecular recognition.

Takahashi T, Nagatoishi S, Kuroda D, Tsumoto K

Molecular basis for governing the morphology of type-I collagen fibrils by Osteomodulin.

Tashima T, Nagatoishi S, Caaveiro JMM, Nakakido M, Sagara H, Kusano-Arai O, Iwanari H, Mimuro H, Hamakubo T, Ohnuma SI, Tsumoto K

Antibody Affinity Maturation by Computational Design.

Kuroda D, Tsumoto K

Structural behavior of keratin-associated protein 8.1 in human hair as revealed by a monoclonal antibody.

Akiba H, Ikeuchi E, Ganbat J, Fujikawa H, Arai-Kusano O, Iwanari H, Nakakido M, Hamakubo T, Shimomura Y, Tsumoto K

Improvement of antibody affinity by introduction of basic amino acid residues into the framework region.

Fukunaga A, Maeta S, Reema B, Nakakido M, Tsumoto K

[Biophysical Analysis of the Protein-Small Molecule Interactions to Develop Small Molecule Drug Discovery].

Nagatoishi S, Caaveiro JMM, Tsumoto K

Discovery and Optimization of Inhibitors of the Parkinson's Disease Associated Protein DJ-1.

Tashiro S, Caaveiro JMM, Nakakido M, Tanabe A, Nagatoishi S, Tamura Y, Matsuda N, Liu D, Hoang QQ, Tsumoto K

Long-Term Stability and Reversible Thermal Unfolding of Antibody Structure at Low pH: Case Study.

Fukada H, Tsumoto K, Arakawa T, Ejima D

Tyrosine Sulfation Restricts the Conformational Ensemble of a Flexible Peptide, Strengthening the Binding Affinity for an Antibody.

Miyanabe K, Yamashita T, Abe Y, Akiba H, Takamatsu Y, Nakakido M, Hamakubo T, Ueda T, Caaveiro JMM, Tsumoto K

Intramolecular H-bonds govern the recognition of a flexible peptide by an antibody.

Miyanabe K, Akiba H, Kuroda D, Nakakido M, Kusano-Arai O, Iwanari H, Hamakubo T, Caaveiro JMM, Tsumoto K

Roles of the disulfide bond between the variable and the constant domains of rabbit immunoglobulin kappa chains in thermal stability and affinity.

Kawade R, Akiba H, Entzminger K, Maruyama T, Okumura CJ, Tsumoto K

Inhibition of homophilic dimerization and disruption of cell adhesion by P-cadherin-specific small molecules from SPR-based assays.

Senoo A, Nagatoishi S, Moberg A, Babol LN, Mitani T, Tashima T, Kudo S, Tsumoto K

Repertoire Analysis of Antibody CDR-H3 Loops Suggests Affinity Maturation Does Not Typically Result in Rigidification.

Jeliazkov JR, Sljoka A, Kuroda D, Tsuchimura N, Katoh N, Tsumoto K, Gray JJ

A combination of ¹⁹F NMR and surface plasmon resonance for site-specific hit selection and validation of fragment molecules that bind to the ATP-binding site of a kinase.

Nagatoishi S, Yamaguchi S, Katoh E, Kajita K, Yokotagawa T, Kanai S, Furuya T, Tsumoto K

Assessing the Heterogeneity of the Fc-Glycan of a Therapeutic Antibody Using an engineered FcγReceptor IIIa-Immobilized Column.

Kiyoshi M, Caaveiro JMM, Tada M, Tamura H, Tanaka T, Terao Y, Morante K, Harazono A, Hashii N, Shibata H, Kuroda D, Nagatoishi S, Oe S, Ide T, Tsumoto K, Ishii-Watabe A

Synergistic Cytotoxic Effect on Gastric Cancer Cells of an Immunotoxin Cocktail in Which Antibodies Recognize Different Epitopes on CDH17.

Kusano-Arai O, Iwanari H, Kudo S, Kikuchi C, Yui A, Akiba H, Matsusaka K, Kaneda A, Fukayama M, Tsumoto K, Hamakubo T

Structures of the prefusion form of measles virus fusion protein in complex with inhibitors.

Hashiguchi T, Fukuda Y, Matsuoka R, Kuroda D, Kubota M, Shirogane Y, Watanabe S, Tsumoto K, Kohda D, Plemper RK, Yanagi Y

Development of drug discovery screening system by molecular interaction kinetics-mass spectrometry.

Obi N, Fukuda T, Nakayama N, Ervin J, Bando Y, Nishimura T, Nagatoishi S, Tsumoto K, Kawamura T

A secondary RET mutation in the activation loop conferring resistance to vandetanib.

Nakaoku T, Kohno T, Araki M, Niho S, Chauhan R, Knowles PP, Tsuchihara K, Matsumoto S, Shimada Y, Mimaki S, Ishii G, Ichikawa H, Nagatoishi S, Tsumoto K, Okuno Y, Yoh K, McDonald NQ, Goto K

Characterization of glycoengineered anti-HER2 monoclonal antibodies produced by using a silkworm-baculovirus expression system.

Egashira Y, Nagatoishi S, Kiyoshi M, Ishii-Watabe A, Tsumoto K

Enzymatically cleavable traceless biotin tags for protein PEGylation and purification.

Wawro AM, Aoki Y, Muraoka T, Tsumoto K, Kinbara K

Production and characterization of a novel site-specific-modifiable anti-OX40-receptor single-chain variable fragment for targeted drug delivery.

Tanabe A, Nakano K, Nakakido M, Nagatoishi S, Tanaka Y, Tsumoto K, Uchimaru K, Watanabe T

Introduction: Antibody-Mediated Therapy Special Issue Part 2.

Nakamura A, Tsumoto K, Takai T

PRDM14 directly interacts with heat shock proteins HSP90α and glucose-regulated protein 78.

Moriya C, Taniguchi H, Nagatoishi S, Igarashi H, Tsumoto K, Imai K

Thermodynamic analyses of amino acid residues at the interface of an antibody B2212A and its antigen roundabout homolog 1.

Yui A, Akiba H, Kudo S, Nakakido M, Nagatoishi S, Tsumoto K

Analysis of Pore Formation and Protein Translocation Using Large Biological Nanopores.

Watanabe H, Gubbiotti A, Chinappi M, Takai N, Tanaka K, Tsumoto K, Kawano R

Structure of the triose-phosphate/phosphate translocator reveals the basis of substrate specificity.

Lee Y, Nishizawa T, Takemoto M, Kumazaki K, Yamashita K, Hirata K, Minoda A, Nagatoishi S, Tsumoto K, Ishitani R, Nureki O

Polymeric SpyCatcher Scaffold Enables Bioconjugation in a Ratio-Controllable Manner.

Jia L, Minamihata K, Ichinose H, Tsumoto K, Kamiya N

Biophysical characterization of the interaction between heme and proteins responsible for heme transfer in Streptococcus pyogenes.

Hoshino M, Nakakido M, Nagatoishi S, Aikawa C, Nakagawa I, Tsumoto K

Structural basis for binding and transfer of heme in bacterial heme-acquisition systems.

Naoe Y, Nakamura N, Rahman MM, Tosha T, Nagatoishi S, Tsumoto K, Shiro Y, Sugimoto H

Elucidation of potential sites for antibody engineering by fluctuation editing.

Yanaka S, Moriwaki Y, Tsumoto K, Sugase K

Insight in the Evolution of Therapeutic Monoclonal Antibodies-Fundamental Technologies for Creation and Evaluation of Next Generation mAbs.

Ishii-Watabe A, Tsumoto K

Glycosylation of IgG-Fc: a molecular perspective.

Kiyoshi M, Tsumoto K, Ishii-Watabe A, Caaveiro JMM

Use of SpyTag/SpyCatcher to construct bispecific antibodies that target two epitopes of a single antigen.

Yumura K, Akiba H, Nagatoishi S, Kusano-Arai O, Iwanari H, Hamakubo T, Tsumoto K

Haemolytic actinoporins interact with carbohydrates using their lipid-binding module.

Tanaka K, Caaveiro JMM, Morante K, Tsumoto K

Functional Contacts between MPER and the Anti-HIV-1 Broadly Neutralizing Antibody 4E10 Extend into the Core of the Membrane.

Rujas E, Insausti S, García-Porras M, Sánchez-Eugenia R, Tsumoto K, Nieva JL, Caaveiro JM

Peripheral Membrane Interactions Boost the Engagement by an Anti-HIV-1 Broadly Neutralizing Antibody.

Rujas E, Caaveiro JM, Insausti S, García-Porras M, Tsumoto K, Nieva JL

The carboxyl-terminal region of Dok-7 plays a key, but not essential, role in activation of muscle-specific receptor kinase MuSK and neuromuscular synapse formation.

Ueta R, Tezuka T, Izawa Y, Miyoshi S, Nagatoishi S, Tsumoto K, Yamanashi Y

Disruption of cell adhesion by an antibody targeting the cell-adhesive intermediate (X-dimer) of human P-cadherin.

Kudo S, Caaveiro JM, Nagatoishi S, Miyafusa T, Matsuura T, Sudou Y, Tsumoto K

Differential Effect of Membrane Composition on the Pore-Forming Ability of Four Different Sea Anemone Actinoporins.

García-Linares S, Rivera-de-Torre E, Morante K, Tsumoto K, Caaveiro JM, Gavilanes JG, Slotte JP, Martínez-Del-Pozo Á

Structural basis for broad neutralization of HIV-1 through the molecular recognition of 10E8 helical epitope at the membrane interface.

Rujas E, Caaveiro JM, Partida-Hanon A, Gulzar N, Morante K, Apellániz B, García-Porras M, Bruix M, Tsumoto K, Scott JK, Jiménez MÁ, Nieva JL

Through-bond effects in the ternary complexes of thrombin sandwiched by two DNA aptamers.

Pica A, Russo Krauss I, Parente V, Tateishi-Karimata H, Nagatoishi S, Tsumoto K, Sugimoto N, Sica F

Production and characterization of genetically modified human IL-11 variants.

Sano E, Takei T, Ueda T, Tsumoto K

Improved Brain Expression of Anti-Amyloid β scFv by Complexation of mRNA Including a Secretion Sequence with PEG-based Block Catiomer.

Perche F, Uchida S, Akiba H, Lin CY, Ikegami M, Dirisala A, Nakashima T, Itaka K, Tsumoto K, Kataoka K

Adhesive Dimerization of Human P-Cadherin Catalyzed by a Chaperone-like Mechanism.

Kudo S, Caaveiro JM, Tsumoto K

In-Cell Enzymology To Probe His-Heme Ligation in Heme Oxygenase Catalysis.

Sigala PA, Morante K, Tsumoto K, Caaveiro JM, Goldberg DE

Identification of a Membrane-bound Prepore Species Clarifies the Lytic Mechanism of Actinoporins.

Morante K, Bellomio A, Gil-Cartón D, Redondo-Morata L, Sot J, Scheuring S, Valle M, González-Mañas JM, Tsumoto K, Caaveiro JMM

Structural basis for amino acid export by DMT superfamily transporter YddG.

Tsuchiya H, Doki S, Takemoto M, Ikuta T, Higuchi T, Fukui K, Usuda Y, Tabuchi E, Nagatoishi S, Tsumoto K, Nishizawa T, Ito K, Dohmae N, Ishitani R, Nureki O

Quantitative phosphoproteomics-based molecular network description for high-resolution kinase-substrate interactome analysis.

Narushima Y, Kozuka-Hata H, Tsumoto K, Inoue J, Oyama M

Reply to "The Broadly Neutralizing, Anti-HIV Antibody 4E10: an Open and Shut Case?".

Rujas E, Gulzar N, Morante K, Tsumoto K, Scott JK, Nieva JL, Caaveiro JM

Assessment of the Protein-Protein Interactions in a Highly Concentrated Antibody Solution by Using Raman Spectroscopy.

Ota C, Noguchi S, Nagatoishi S, Tsumoto K

Integrative Network Analysis Combined with Quantitative Phosphoproteomics Reveals Transforming Growth Factor-beta Receptor type-2 (TGFBR2) as a Novel Regulator of Glioblastoma Stem Cell Properties.

Narushima Y, Kozuka-Hata H, Koyama-Nasu R, Tsumoto K, Inoue J, Akiyama T, Oyama M

Rapid Heme Transfer Reactions between NEAr Transporter Domains of Staphylococcus aureus: A Theoretical Study Using QM/MM and MD Simulations.

Moriwaki Y, Terada T, Tsumoto K, Shimizu K

Epiregulin Recognition Mechanisms by Anti-epiregulin Antibody 9E5: STRUCTURAL, FUNCTIONAL, AND MOLECULAR DYNAMICS SIMULATION ANALYSES.

Kado Y, Mizohata E, Nagatoishi S, Iijima M, Shinoda K, Miyafusa T, Nakayama T, Yoshizumi T, Sugiyama A, Kawamura T, Lee YH, Matsumura H, Doi H, Fujitani H, Kodama T, Shibasaki Y, Tsumoto K, Inoue T

Mitochonic Acid 5 Binds Mitochondria and Ameliorates Renal Tubular and Cardiac Myocyte Damage.

Suzuki T, Yamaguchi H, Kikusato M, Hashizume O, Nagatoishi S, Matsuo A, Sato T, Kudo T, Matsuhashi T, Murayama K, Ohba Y, Watanabe S, Kanno S, Minaki D, Saigusa D, Shinbo H, Mori N, Yuri A, Yokoro M, Mishima E, Shima H, Akiyama Y, Takeuchi Y, Kikuchi K, Toyohara T, Suzuki C, Ichimura T, Anzai J, Kohzuki M, Mano N, Kure S, Yanagisawa T, Tomioka Y, Toyomizu M, Tsumoto K, Nakada K, Bonventre JV, Ito S, Osaka H, Hayashi K, Abe T

Bidirectional Transformation of a Metamorphic Protein between the Water-Soluble and Transmembrane Native States.

Tanaka K, Caaveiro JM, Tsumoto K

Structural basis for inhibition of the Tob-CNOT7 interaction by a fragment screening approach.

Bai Y, Tashiro S, Nagatoishi S, Suzuki T, Yan D, Liu R, Tsumoto K, Bartlam M, Yamamoto T

Structural analysis of Fc/FcγR complexes: a blueprint for antibody design.

Caaveiro JM, Kiyoshi M, Tsumoto K

Discovery and characterization of natural tropolones as inhibitors of the antibacterial target CapF from Staphylococcus aureus.

Nakano K, Chigira T, Miyafusa T, Nagatoishi S, Caaveiro JM, Tsumoto K

Structural and Thermodynamic Basis of Epitope Binding by Neutralizing and Nonneutralizing Forms of the Anti-HIV-1 Antibody 4E10.

Rujas E, Gulzar N, Morante K, Tsumoto K, Scott JK, Nieva JL, Caaveiro JM

Differential Effects of IFN-β on the Survival and Growth of Human Vascular Smooth Muscle and Endothelial Cells.

Sano E, Tashiro S, Tsumoto K, Ueda T

Thermodynamic characterization of the interaction between the human Y-box binding protein YB-1 and nucleic acids.

Tanabe Y, Nagatoishi S, Tsumoto K

Functional characterization of Val60, a key residue involved in the membrane-oligomerization of fragaceatoxin C, an actinoporin from Actinia fragacea.

Morante K, Caaveiro JM, Viguera AR, Tsumoto K, González-Mañas JM

Differential binding of prohibitin-2 to estrogen receptor α and to drug-resistant ERα mutants.

Chigira T, Nagatoishi S, Tsumoto K

Osteomodulin regulates diameter and alters shape of collagen fibrils.

Tashima T, Nagatoishi S, Sagara H, Ohnuma S, Tsumoto K

Thermodynamics of antibody-antigen interaction revealed by mutation analysis of antibody variable regions.

Akiba H, Tsumoto K

Structural basis for binding of human IgG1 to its high-affinity human receptor FcγRI.

Kiyoshi M, Caaveiro JM, Kawai T, Tashiro S, Ide T, Asaoka Y, Hatayama K, Tsumoto K

Protein stabilization by an amphiphilic short monodisperse oligo(ethylene glycol).

Sadhukhan N, Muraoka T, Ui M, Nagatoishi S, Tsumoto K, Kinbara K

The Atomic Structure of the HIV-1 gp41 Transmembrane Domain and Its Connection to the Immunogenic Membrane-proximal External Region.

Apellániz B, Rujas E, Serrano S, Morante K, Tsumoto K, Caaveiro JM, Jiménez MÁ, Nieva JL

A pore-forming toxin requires a specific residue for its activity in membranes with particular physicochemical properties.

Morante K, Caaveiro JM, Tanaka K, González-Mañas JM, Tsumoto K

Click conjugation of a binuclear terbium(III) complex for real-time detection of tyrosine phosphorylation.

Akiba H, Sumaoka J, Tsumoto K, Komiyama M

Structural basis for self-assembly of a cytolytic pore lined by protein and lipid.

Tanaka K, Caaveiro JM, Morante K, González-Mañas JM, Tsumoto K

pH responsiveness of fibrous assemblies of repeat-sequence amphipathic α-helix polypeptides.

Takei T, Tsumoto K, Okonogi A, Kimura A, Kojima S, Yazaki K, Takei T, Ueda T, Miura K

Revealing the peptide presenting process of human leukocyte antigen through the analysis of fluctuation.

Yanaka S, Ueno T, Tsumoto K, Sugase K

Structural features of interfacial tyrosine residue in ROBO1 fibronectin domain-antibody complex: Crystallographic, thermodynamic, and molecular dynamic analyses.

Nakayama T, Mizohata E, Yamashita T, Nagatoishi S, Nakakido M, Iwanari H, Mochizuki Y, Kado Y, Yokota Y, Satoh R, Tsumoto K, Fujitani H, Kodama T, Hamakubo T, Inoue T

Quantitative laser diffraction method for the assessment of protein subvisible particles.

Totoki S, Yamamoto G, Tsumoto K, Uchiyama S, Fukui K

Crystal structure of streptavidin mutant with low immunogenicity.

Kawato T, Mizohata E, Meshizuka T, Doi H, Kawamura T, Matsumura H, Yumura K, Tsumoto K, Kodama T, Inoue T, Sugiyama A

The molecular interaction of a protein in highly concentrated solution investigated by Raman spectroscopy.

Ota C, Noguchi S, Tsumoto K

Efficacy of ribavirin against malignant glioma cell lines.

Ogino A, Sano E, Ochiai Y, Yamamuro S, Tashiro S, Yachi K, Ohta T, Fukushima T, Okamoto Y, Tsumoto K, Ueda T, Yoshino A, Katayama Y

Preparation and characterization of anti-tissue factor single-chain variable fragment antibody for cancer diagnosis.

Sato R, Obonai T, Tsumura R, Tsumoto K, Koga Y, Yasunaga M, Matsumura Y

Reversible ion transportation switch by a ligand-gated synthetic supramolecular ion channel.

Muraoka T, Endo T, Tabata KV, Noji H, Nagatoishi S, Tsumoto K, Li R, Kinbara K

Recent advances in antibody engineering.

Tsumoto K, Hagihara Y, Saerens D

Grafting synthetic transmembrane units to the engineered low-toxicity α-hemolysin to restore its hemolytic activity.

Ui M, Harima K, Takei T, Tsumoto K, Tabata KV, Noji H, Endo S, Akiyama K, Muraoka T, Kinbara K

Effects of syringe material and silicone oil lubrication on the stability of pharmaceutical proteins.

Krayukhina E, Tsumoto K, Uchiyama S, Fukui K

Protective effect of the long pentraxin PTX3 against histone-mediated endothelial cell cytotoxicity in sepsis.

Daigo K, Nakakido M, Ohashi R, Fukuda R, Matsubara K, Minami T, Yamaguchi N, Inoue K, Jiang S, Naito M, Tsumoto K, Hamakubo T

Peptide-dependent conformational fluctuation determines the stability of the human leukocyte antigen class I complex.

Yanaka S, Ueno T, Shi Y, Qi J, Gao GF, Tsumoto K, Sugase K

Next generation drying technologies for pharmaceutical applications.

Walters RH, Bhatnagar B, Tchessalov S, Izutsu KI, Tsumoto K, Ohtake S

Alternative downstream processes for production of antibodies and antibody fragments.

Arakawa T, Tsumoto K, Ejima D

The staphylococcal elastin-binding protein regulates zinc-dependent growth/biofilm formation.

Nakakido M, Aikawa C, Nakagawa I, Tsumoto K

Thermodynamic and structural characterization of the specific binding of Zn(II) to human protein DJ-1.

Tashiro S, Caaveiro JM, Wu CX, Hoang QQ, Tsumoto K

Role of positions e and g in the fibrous assembly formation of an amphipathic α-helix-forming polypeptide.

Takei T, Tsumoto K, Yoshino M, Kojima S, Yazaki K, Ueda T, Takei T, Arisaka F, Miura K

Identification and characterization of the X-dimer of human P-cadherin: implications for homophilic cell adhesion.

Kudo S, Caaveiro JM, Goda S, Nagatoishi S, Ishii K, Matsuura T, Sudou Y, Kodama T, Hamakubo T, Tsumoto K

Affinity improvement of a therapeutic antibody by structure-based computational design: generation of electrostatic interactions in the transition state stabilizes the antibody-antigen complex.

Kiyoshi M, Caaveiro JM, Miura E, Nagatoishi S, Nakakido M, Soga S, Shirai H, Kawabata S, Tsumoto K

Heme binding mechanism of structurally similar iron-regulated surface determinant near transporter domains of Staphylococcus aureus exhibiting different affinities for heme.

Moriwaki Y, Terada T, Caaveiro JM, Takaoka Y, Hamachi I, Tsumoto K, Shimizu K

Improving the affinity of an antibody for its antigen via long-range electrostatic interactions.

Fukunaga A, Tsumoto K

Dynamic elements govern the catalytic activity of CapE, a capsular polysaccharide-synthesizing enzyme from Staphylococcus aureus.

Miyafusa T, Caaveiro JM, Tanaka Y, Tsumoto K

Discovery of an uncovered region in fibrin clots and its clinical significance.

Hisada Y, Yasunaga M, Hanaoka S, Saijou S, Sugino T, Tsuji A, Saga T, Tsumoto K, Manabe S, Kuroda J, Kuratsu J, Matsumura Y

Effects of subclass change on the structural stability of chimeric, humanized, and human antibodies under thermal stress.

Ito T, Tsumoto K

Hyperthin nanochains composed of self-polymerizing protein shackles.

Matsunaga R, Yanaka S, Nagatoishi S, Tsumoto K

Bidirectional binding property of high glycine-tyrosine keratin-associated protein contributes to the mechanical strength and shape of hair.

Matsunaga R, Abe R, Ishii D, Watanabe SI, Kiyoshi M, Nöcker B, Tsuchiya M, Tsumoto K

Selective binding of antimicrobial porphyrins to the heme-receptor IsdH-NEAT3 of Staphylococcus aureus.

Vu NT, Moriwaki Y, Caaveiro JM, Terada T, Tsutsumi H, Hamachi I, Shimizu K, Tsumoto K

Crystal structure of the capsular polysaccharide synthesizing protein CapE of Staphylococcus aureus.

Miyafusa T, Caaveiro JM, Tanaka Y, Tanner ME, Tsumoto K

Heme degradation by Staphylococcus aureus IsdG and IsdI liberates formaldehyde rather than carbon monoxide.

Matsui T, Nambu S, Ono Y, Goulding CW, Tsumoto K, Ikeda-Saito M

Effects of chain length of an amphipathic polypeptide carrying the repeated amino acid sequence (LETLAKA)(n) on α-helix and fibrous assembly formation.

Takei T, Hasegawa K, Imada K, Namba K, Tsumoto K, Kuriki Y, Yoshino M, Yazaki K, Kojima S, Takei T, Ueda T, Miura K

Incorporation of rapid thermodynamic data in fragment-based drug discovery.

Kobe A, Caaveiro JM, Tashiro S, Kajihara D, Kikkawa M, Mitani T, Tsumoto K

Identification of small-molecule inhibitors of the human S100B-p53 interaction and evaluation of their activity in human melanoma cells.

Yoshimura C, Miyafusa T, Tsumoto K

Secretory production of single-chain antibody (scFv) in Brevibacillus choshinensis using novel fusion partner.

Tokunaga M, Mizukami M, Yamasaki K, Tokunaga H, Onishi H, Hanagata H, Ishibashi M, Miyauchi A, Tsumoto K, Arakawa T

Mutations for decreasing the immunogenicity and maintaining the function of core streptavidin.

Yumura K, Ui M, Doi H, Hamakubo T, Kodama T, Tsumoto K, Sugiyama A

Crystal structure of the C-terminal domain of Mu phage central spike and functions of bound calcium ion.

Harada K, Yamashita E, Nakagawa A, Miyafusa T, Tsumoto K, Ueno T, Toyama Y, Takeda S

Phosphoproteome of human glioblastoma initiating cells reveals novel signaling regulators encoded by the transcriptome.

Kozuka-Hata H, Nasu-Nishimura Y, Koyama-Nasu R, Ao-Kondo H, Tsumoto K, Akiyama T, Oyama M

Type 1 IFN inhibits the growth factor deprived apoptosis of cultured human aortic endothelial cells and protects the cells from chemically induced oxidative cytotoxicity.

Sano E, Tashiro S, Tadakuma H, Takei T, Ueda T, Tsumoto K

Thermodynamic analysis of a multifunctional RNA-binding protein, PhoRpp38, in the hyperthermophilic archaeon Pyrococcus horikoshii OT3.

Oshima K, Nakashima T, Kakuta Y, Tsumoto K, Kimura M

Structural and thermodynamic characterization of the self-adhesive properties of human P-cadherin.

Kudo S, Caaveiro JM, Miyafusa T, Goda S, Ishii K, Matsuura T, Sudou Y, Kodama T, Hamakubo T, Tsumoto K

Application of photoactive yellow protein as a photoresponsive module for controlling hemolytic activity of staphylococcal α-hemolysin.

Ui M, Tanaka Y, Araki Y, Wada T, Takei T, Tsumoto K, Endo S, Kinbara K

Establishment of a microcarrier culture system with serial sub-cultivation for functionally active human endothelial cells.

Tashiro S, Tsumoto K, Sano E

Mapping ultra-weak protein-protein interactions between heme transporters of Staphylococcus aureus.

Abe R, Caaveiro JM, Kozuka-Hata H, Oyama M, Tsumoto K

Crystal structure of the enzyme CapF of Staphylococcus aureus reveals a unique architecture composed of two functional domains.

Miyafusa T, Caaveiro JM, Tanaka Y, Tsumoto K

Interleukin-11 links oxidative stress and compensatory proliferation.

Nishina T, Komazawa-Sakon S, Yanaka S, Piao X, Zheng DM, Piao JH, Kojima Y, Yamashina S, Sano E, Putoczki T, Doi T, Ueno T, Ezaki J, Ushio H, Ernst M, Tsumoto K, Okumura K, Nakano H

Catalytic activity of MsbA reconstituted in nanodisc particles is modulated by remote interactions with the bilayer.

Kawai T, Caaveiro JM, Abe R, Katagiri T, Tsumoto K

Gene expression profiles predicting the response to IFN-β and a combination of temozolomide and IFN-β in malignant gliomas.

Yoshino A, Tashiro S, Ogino A, Yachi K, Ohta T, Fukushima T, Watanabe T, Katayama Y, Okamoto Y, Sano E, Tsumoto K

Molecular basis of recognition of antibacterial porphyrins by heme-transporter IsdH-NEAT3 of Staphylococcus aureus.

Moriwaki Y, Caaveiro JM, Tanaka Y, Tsutsumi H, Hamachi I, Tsumoto K

Hydration is required in DNA G-quadruplex-protein binding.

Nagatoishi S, Isono N, Tsumoto K, Sugimoto N

Monoubiquitination of Tob/BTG family proteins competes with degradation-targeting polyubiquitination.

Suzuki T, Kim M, Kozuka-Hata H, Watanabe M, Oyama M, Tsumoto K, Yamamoto T

Loop residues of thrombin-binding DNA aptamer impact G-quadruplex stability and thrombin binding.

Nagatoishi S, Isono N, Tsumoto K, Sugimoto N

Stepwise characterization of the thermodynamics of trichocyte intermediate filament protein supramolecular assembly.

Ishii D, Abe R, Watanabe S, Tsuchiya M, Nöcker B, Tsumoto K

Refolding single-chain antibody (scFv) using lauroyl-L-glutamate as a solubilization detergent and arginine as a refolding additive.

Kudou M, Ejima D, Sato H, Yumioka R, Arakawa T, Tsumoto K

An approach to rational ligand-design based on a thermodynamic analysis.

Ui M, Tsumoto K

Structural and energetic hot-spots for the interaction between a ladder-like polycyclic ether and the anti-ciguatoxin antibody 10C9Fab.

Ui M, Tanaka Y, Tsumuraya T, Fujii I, Inoue M, Hirama M, Tsumoto K

Non-core region modulates interleukin-11 signaling activity: generation of agonist and antagonist variants.

Yanaka S, Sano E, Naruse N, Miura K, Futatsumori-Sugai M, Caaveiro JM, Tsumoto K

Chemical and pharmacological chaperones: application for recombinant protein production and protein folding diseases.

Rajan RS, Tsumoto K, Tokunaga M, Tokunaga H, Kita Y, Arakawa T

Salicylate restores transport function and anion exchanger activity of missense pendrin mutations.

Ishihara K, Okuyama S, Kumano S, Iida K, Hamana H, Murakoshi M, Kobayashi T, Usami S, Ikeda K, Haga Y, Tsumoto K, Nakamura H, Hirasawa N, Wada H

A novel protein refolding system using lauroyl-l-glutamate as a solubilizing detergent and arginine as a folding assisting agent.

Kudou M, Yumioka R, Ejima D, Arakawa T, Tsumoto K

The structural mechanism of the inhibition of archaeal RelE toxin by its cognate RelB antitoxin.

Shinohara M, Guo JX, Mori M, Nakashima T, Takagi H, Nishimoto E, Yamashita S, Tsumoto K, Kakuta Y, Kimura M

Expression of antibody variable region-human alkaline phosphatase fusion proteins in mammalian cells.

Sasajima Y, Iwasaki R, Tsumoto K, Kumagai I, Ihara M, Ueda H

Gene expression profiling predicts response to temozolomide in malignant gliomas.

Yoshino A, Ogino A, Yachi K, Ohta T, Fukushima T, Watanabe T, Katayama Y, Okamoto Y, Naruse N, Sano E, Tsumoto K

Salicylate-induced translocation of prestin having mutation in the GTSRH sequence to the plasma membrane.

Kumano S, Iida K, Ishihara K, Murakoshi M, Tsumoto K, Ikeda K, Kumagai I, Kobayashi T, Wada H

Solubilization of membrane proteins with novel N-acylamino acid detergents.

Abe R, Caaveiro JM, Kudou M, Tsumoto K

Step-wise refolding of recombinant proteins.

Tsumoto K, Arakawa T, Chen L

Non-denaturing solubilization of inclusion bodies.

Tsumoto K, Abe R, Ejima D, Arakawa T

Contribution of the flexible loop region to the function of staphylococcal enterotoxin B.

Yanaka S, Kudou M, Tanaka Y, Sasaki T, Takemoto S, Sakata A, Hattori Y, Koshi T, Futaki S, Tsumoto K, Nakashima T

Reactive oxygen species induced by Streptococcus pyogenes invasion trigger apoptotic cell death in infected epithelial cells.

Aikawa C, Nozawa T, Maruyama F, Tsumoto K, Hamada S, Nakagawa I

Temperature and salt concentration alter base-sequence selectivity of a duplex DNA-binding protein.

Nagatoishi S, Tanaka Y, Kudou M, Tsumoto K

Contribution of asparagine residues to the stabilization of a proteinaceous antigen-antibody complex, HyHEL-10-hen egg white lysozyme.

Yokota A, Tsumoto K, Shiroishi M, Nakanishi T, Kondo H, Kumagai I

Direct and selective immobilization of proteins by means of an inorganic material-binding peptide: discussion on functionalization in the elongation to material-binding peptide.

Yokoo N, Togashi T, Umetsu M, Tsumoto K, Hattori T, Nakanishi T, Ohara S, Takami S, Naka T, Abe H, Kumagai I, Adschiri T

Signal peptide design for improving recombinant protein secretion in the baculovirus expression vector system.

Futatsumori-Sugai M, Tsumoto K

MEP HyperCel chromatography II: binding, washing and elution.

Arakawa T, Futatsumori-Sugai M, Tsumoto K, Kita Y, Sato H, Ejima D

[Rational fragment-design method based on a thermodynamic analysis].

Tsumoto K, Ui M

Screening of effective column rinse solvent for Protein-A chromatography.

Yumioka R, Tsumoto K, Arakawa T, Ejima D

Observation of the membrane binding activity and domain structure of gpV, which comprises the tail spike of bacteriophage P2.

Kageyama Y, Murayama M, Onodera T, Yamada S, Fukada H, Kudou M, Tsumoto K, Toyama Y, Kado S, Kubota K, Takeda S

The interaction of hyperthermophilic TATA-box binding protein with single-stranded DNA is entropically favorable and exhibits a large negative heat capacity change at high salt concentration.

Nagatoishi S, Tanaka Y, Kudou M, Tsumoto K

[Recognition mechanism for poly-cyclic ether toxin by anti-ciguatoxin antibody].

Ui M, Tsumoto K

To be excluded or to bind, that is the question: Arginine effects on proteins.

Nakakido M, Kudou M, Arakawa T, Tsumoto K

Contributions of interfacial residues of human Interleukin15 to the specificity and affinity for its private alpha-receptor.

Sakamoto S, Caaveiro JM, Sano E, Tanaka Y, Kudou M, Tsumoto K

Impact of intrinsic cooperative thermodynamics of peptide-MHC complexes on antiviral activity of HIV-specific CTL.

Motozono C, Yanaka S, Tsumoto K, Takiguchi M, Ueno T

Utilization of Arg-elution method for FLAG-tag based chromatography.

Futatsumori-Sugai M, Abe R, Watanabe M, Kudou M, Yamamoto T, Ejima D, Arakawa T, Tsumoto K

Immobilized metal affinity chromatography in the presence of arginine.

Abe R, Kudou M, Tanaka Y, Arakawa T, Tsumoto K

Molecular glues carrying multiple guanidinium ion pendants via an oligoether spacer: stabilization of microtubules against depolymerization.

Okuro K, Kinbara K, Tsumoto K, Ishii N, Aida T

Activation of leukemia-associated RhoGEF by Galpha13 with significant conformational rearrangements in the interface.

Suzuki N, Tsumoto K, Hajicek N, Daigo K, Tokita R, Minami S, Kodama T, Hamakubo T, Kozasa T

Growth control of hybridoma cells with an artificially induced EpoR-gp130 heterodimer.

Kawahara M, Ogo Y, Tsumoto K, Kumagai I, Ueda H, Nagamune T

Electron microscopy and computational studies of Ebh, a giant cell-wall-associated protein from Staphylococcus aureus.

Sakamoto S, Tanaka Y, Tanaka I, Takei T, Yu J, Kuroda M, Yao M, Ohta T, Tsumoto K

Structure-based analysis reveals hydration changes induced by arginine hydrochloride.

Nakakido M, Tanaka Y, Mitsuhori M, Kudou M, Ejima D, Arakawa T, Tsumoto K

Structural basis for multimeric heme complexation through a specific protein-heme interaction: the case of the third neat domain of IsdH from Staphylococcus aureus.

Watanabe M, Tanaka Y, Suenaga A, Kuroda M, Yao M, Watanabe N, Arisaka F, Ohta T, Tanaka I, Tsumoto K

Staphylococcus aureus giant protein Ebh is involved in tolerance to transient hyperosmotic pressure.

Kuroda M, Tanaka Y, Aoki R, Shu D, Tsumoto K, Ohta T

Selective expansion of genetically modified T cells using an antibody/interleukin-2 receptor chimera.

Sogo T, Kawahara M, Tsumoto K, Kumagai I, Ueda H, Nagamune T

Expression, purification, crystallization and preliminary diffraction analysis of CapF, a capsular polysaccharide-synthesis enzyme from Staphylococcus aureus.

Miyafusa T, Tanaka Y, Kuroda M, Ohta T, Tsumoto K

How protein recognizes ladder-like polycyclic ethers. Interactions between ciguatoxin (CTX3C) fragments and its specific antibody 10C9.

Ui M, Tanaka Y, Tsumuraya T, Fujii I, Inoue M, Hirama M, Tsumoto K

Thermodynamic analysis reveals that GTP binding affects the interaction between the alpha- and gamma-subunits of translation initiation factor 2.

Nakakido M, Tanaka Y, Sokabe M, Tsumoto K

A helical string of alternately connected three-helix bundles for the cell wall-associated adhesion protein Ebh from Staphylococcus aureus.

Tanaka Y, Sakamoto S, Kuroda M, Goda S, Gao YG, Tsumoto K, Hiragi Y, Yao M, Watanabe N, Ohta T, Tanaka I

Critical contribution of aromatic rings to specific recognition of polyether rings. The case of ciguatoxin CTX3C-ABC and its specific antibody 1C49.

Tsumoto K, Yokota A, Tanaka Y, Ui M, Tsumuraya T, Fujii I, Kumagai I, Nagumo Y, Oguri H, Inoue M, Hirama M

[Solvent modulation of chromatography].

Arakawa T, Ejima D, Tsumoto K, Gagnon P

Effect of an amyloidogenic sequence attached to yellow fluorescent protein.

Hamada D, Tsumoto K, Sawara M, Tanaka N, Nakahira K, Shiraki K, Yanagihara I

Critical contribution of VH-VL interaction to reshaping of an antibody: the case of humanization of anti-lysozyme antibody, HyHEL-10.

Nakanishi T, Tsumoto K, Yokota A, Kondo H, Kumagai I

Grafting of material-binding function into antibodies Functionalization by peptide grafting.

Hattori T, Umetsu M, Nakanishi T, Tsumoto K, Ohara S, Abe H, Naito M, Asano R, Adschiri T, Kumagai I

Thermodynamic consequences of mutations in vernier zone residues of a humanized anti-human epidermal growth factor receptor murine antibody, 528.

Makabe K, Nakanishi T, Tsumoto K, Tanaka Y, Kondo H, Umetsu M, Sone Y, Asano R, Kumagai I

Human CD4+ central and effector memory T cells produce IL-21: effect on cytokine-driven proliferation of CD4+ T cell subsets.

Onoda T, Rahman M, Nara H, Araki A, Makabe K, Tsumoto K, Kumagai I, Kudo T, Ishii N, Tanaka N, Sugamura K, Hayasaka K, Asao H

Crystal structure analysis reveals a novel forkhead-associated domain of ESAT-6 secretion system C protein in Staphylococcus aureus.

Tanaka Y, Kuroda M, Yasutake Y, Yao M, Tsumoto K, Watanabe N, Ohta T, Tanaka I

The N-terminal domain of elastin-binding protein of Staphylococcus aureus changes its secondary structure in a membrane-mimetic environment.

Nakakido M, Tanaka Y, Tsumoto K

Molecular properties of two proteins homologous to PduO-type ATP:cob(I)alamin adenosyltransferase from Sulfolobus tokodaii.

Tanaka Y, Sasaki T, Kumagai I, Yasutake Y, Yao M, Tanaka I, Tsumoto K

Arginine improves protein elution in hydrophobic interaction chromatography. The cases of human interleukin-6 and activin-A.

Tsumoto K, Ejima D, Nagase K, Arakawa T

Mimicry of erythropoietin and interleukin-6 signalling by an antibody/cytokine receptor chimera in murine myeloid 32D cells.

Kawahara M, Ueda H, Tsumoto K, Kumagai I, Nagamune T

The effects of arginine on protein binding and elution in hydrophobic interaction and ion-exchange chromatography.

Arakawa T, Tsumoto K, Nagase K, Ejima D

A human antibody fragment with high affinity for biodegradable polymer film.

Watanabe H, Tsumoto K, Taguchi S, Yamashita K, Doi Y, Nishimiya Y, Kondo H, Umetsu M, Kumagai I

Suppression of protein interactions by arginine: a proposed mechanism of the arginine effects.

Arakawa T, Ejima D, Tsumoto K, Obeyama N, Tanaka Y, Kita Y, Timasheff SN

Effects of salts on protein-surface interactions: applications for column chromatography.

Tsumoto K, Ejima D, Senczuk AM, Kita Y, Arakawa T

Induced binding of proteins by ammonium sulfate in affinity and ion-exchange column chromatography.

Arakawa T, Tsumoto K, Ejima D, Kita Y, Yonezawa Y, Tokunaga M

Structural and mutational analyses of Drp35 from Staphylococcus aureus: a possible mechanism for its lactonase activity.

Tanaka Y, Morikawa K, Ohki Y, Yao M, Tsumoto K, Watanabe N, Ohta T, Tanaka I

Structural consequences of mutations in interfacial Tyr residues of a protein antigen-antibody complex. The case of HyHEL-10-HEL.

Shiroishi M, Tsumoto K, Tanaka Y, Yokota A, Nakanishi T, Kondo H, Kumagai I

Effects of acid exposure on the conformation, stability, and aggregation of monoclonal antibodies.

Ejima D, Tsumoto K, Fukada H, Yumioka R, Nagase K, Arakawa T, Philo JS

Circular dichroism spectra demonstrate formation of the thrombin-binding DNA aptamer G-quadruplex under stabilizing-cation-deficient conditions.

Nagatoishi S, Tanaka Y, Tsumoto K

Improved performance of column chromatography by arginine: dye-affinity chromatography.

Arakawa T, Ejima D, Tsumoto K, Ishibashi M, Tokunaga M

Aggregation suppression of proteins by arginine during thermal unfolding.

Arakawa T, Kita Y, Ejima D, Tsumoto K, Fukada H

Structural basis for recognition of the nonclassical MHC molecule HLA-G by the leukocyte Ig-like receptor B2 (LILRB2/LIR2/ILT4/CD85d).

Shiroishi M, Kuroki K, Rasubala L, Tsumoto K, Kumagai I, Kurimoto E, Kato K, Kohda D, Maenaka K

Small molecule pharmacological chaperones: From thermodynamic stabilization to pharmaceutical drugs.

Arakawa T, Ejima D, Kita Y, Tsumoto K

Humanization of the bispecific epidermal growth factor receptor x CD3 diabody and its efficacy as a potential clinical reagent.

Asano R, Sone Y, Makabe K, Tsumoto K, Hayashi H, Katayose Y, Unno M, Kudo T, Kumagai I

Imaging by atomic force microscopy of the plasma membrane of prestin-transfected Chinese hamster ovary cells.

Murakoshi M, Gomi T, Iida K, Kumano S, Tsumoto K, Kumagai I, Ikeda K, Kobayashi T, Wada H

[Dissection of specificity and affinity of an antibody for its specific antigen].

Tsumoto K

Characterization of arginine as a solvent additive: a halophilic enzyme as a model protein.

Ishibashi M, Tsumoto K, Ejima D, Arakawa T, Tokunaga M

Review: Why is arginine effective in suppressing aggregation?

Tsumoto K, Ejima D, Kita Y, Arakawa T

Efficient leukocyte Ig-like receptor signaling and crystal structure of disulfide-linked HLA-G dimer.

Shiroishi M, Kuroki K, Ose T, Rasubala L, Shiratori I, Arase H, Tsumoto K, Kumagai I, Kohda D, Maenaka K

Entropically driven MHC class I recognition by human inhibitory receptor leukocyte Ig-like receptor B1 (LILRB1/ILT2/CD85j).

Shiroishi M, Kuroki K, Tsumoto K, Yokota A, Sasaki T, Amano K, Shimojima T, Shirakihara Y, Rasubala L, van der Merwe PA, Kumagai I, Kohda D, Maenaka K

Arginine as an effective additive in gel permeation chromatography.

Ejima D, Yumioka R, Arakawa T, Tsumoto K

Selection of highly productive mammalian cells based on an inducible growth advantage using an antibody/receptor chimera.

Kawahara M, Ueda H, Tsumoto K, Kumagai I, Mahoney W, Nagamune T

A novel "reverse screening" to identify refolding additives for activin-A.

Ejima D, Ono K, Tsumoto K, Arakawa T, Eto Y

Crystal structure of the hypothetical protein ST2072 from Sulfolobus tokodaii.

Tanaka Y, Tsumoto K, Tanabe E, Yasutake Y, Sakai N, Yao M, Tanaka I, Kumagai I

Effective elution of antibodies by arginine and arginine derivatives in affinity column chromatography.

Ejima D, Yumioka R, Tsumoto K, Arakawa T

Construction of an expression system for the motor protein prestin in Chinese hamster ovary cells.

Iida K, Tsumoto K, Ikeda K, Kumagai I, Kobayashi T, Wada H

Kinetics of ultrasonic disinfection of Escherichia coli in the presence of titanium dioxide particles.

Kubo M, Onodera R, Shibasaki-Kitakawa N, Tsumoto K, Yonemoto T

Is arginine a protein-denaturant?

Ishibashi M, Tsumoto K, Tokunaga M, Ejima D, Kita Y, Arakawa T

Herpes simplex virus targeting to the EGF receptor by a gD-specific soluble bridging molecule.

Nakano K, Asano R, Tsumoto K, Kwon H, Goins WF, Kumagai I, Cohen JB, Glorioso JC

Nondenaturing solubilization of beta2 microglobulin from inclusion bodies by L-arginine.

Umetsu M, Tsumoto K, Nitta S, Adschiri T, Ejima D, Arakawa T, Kumagai I

Tumor-directed lymphocyte-activating cytokines: refolding-based preparation of recombinant human interleukin-12 and an antibody variable domain-fused protein by additive-introduced stepwise dialysis.

Makabe K, Asano R, Ito T, Tsumoto K, Kudo T, Kumagai I

Role of arginine in protein refolding, solubilization, and purification.

Tsumoto K, Umetsu M, Kumagai I, Ejima D, Philo JS, Arakawa T

Structural evidence for guanidine-protein side chain interactions: crystal structure of CutA from Pyrococcus horikoshii in 3 M guanidine hydrochloride.

Tanaka Y, Tsumoto K, Umetsu M, Nakanishi T, Yasutake Y, Sakai N, Yao M, Tanaka I, Arakawa T, Kumagai I

Phage-display selection of antibodies to the left end of CTX3C using synthetic fragments.

Nagumo Y, Oguri H, Tsumoto K, Shindo Y, Hirama M, Tsumuraya T, Fujii I, Tomioka Y, Mizugaki M, Kumagai I

Elution of antibodies from a Protein-A column by aqueous arginine solutions.

Arakawa T, Philo JS, Tsumoto K, Yumioka R, Ejima D

Reversal of antigen-dependent signaling by two mutations in antibody/receptor chimera: implication of inverse agonism in cytokine receptor superfamily.

Kawahara M, Ishii S, Tsumoto K, Kumagai I, Ueda H, Nagamune T

How oligomerization contributes to the thermostability of an archaeon protein. Protein L-isoaspartyl-O-methyltransferase from Sulfolobus tokodaii.

Tanaka Y, Tsumoto K, Yasutake Y, Umetsu M, Yao M, Fukada H, Tanaka I, Kumagai I

Fucose depletion from human IgG1 oligosaccharide enhances binding enthalpy and association rate between IgG1 and FcgammaRIIIa.

Okazaki A, Shoji-Hosaka E, Nakamura K, Wakitani M, Uchida K, Kakita S, Tsumoto K, Kumagai I, Shitara K

Engineering amyloidogenicity towards the development of nanofibrillar materials.

Hamada D, Yanagihara I, Tsumoto K

Structural characteristics and refolding of in vivo aggregated hyperthermophilic archaeon proteins.

Umetsu M, Tsumoto K, Ashish K, Nitta S, Tanaka Y, Adschiri T, Kumagai I

AMEGA: antigen-mediated genetically modified cell amplification.

Kawahara M, Ueda H, Tsumoto K, Kumagai I, Nagamune T

Structural implications for heavy metal-induced reversible assembly and aggregation of a protein: the case of Pyrococcus horikoshii CutA.

Tanaka Y, Tsumoto K, Nakanishi T, Yasutake Y, Sakai N, Yao M, Tanaka I, Kumagai I

[A magic agent in protein refolding: arginine].

Arakawa T, Katsuyama I, Kumagai I, Tsumoto K

Solubilization of active green fluorescent protein from insoluble particles by guanidine and arginine.

Tsumoto K, Umetsu M, Kumagai I, Ejima D, Arakawa T

A highly effective and stable bispecific diabody for cancer immunotherapy: cure of xenografted tumors by bispecific diabody and T-LAK cells.

Hayashi H, Asano R, Tsumoto K, Katayose Y, Suzuki M, Unno M, Kodama H, Takemura S, Yoshida H, Makabe K, Imai K, Matsuno S, Kumagai I, Kudo T

An optimized homogeneous noncompetitive immunoassay based on the antigen-driven enzymatic complementation.

Ueda H, Yokozeki T, Arai R, Tsumoto K, Kumagai I, Nagamune T

On-column refolding and characterization of soluble human interleukin-15 receptor alpha-chain produced in Escherichia coli.

Matsumoto M, Misawa S, Tsumoto K, Kumagai I, Hayashi H, Kobayashi Y

Immobilized oxidoreductase as an additive for refolding inclusion bodies: application to antibody fragments.

Tsumoto K, Umetsu M, Yamada H, Ito T, Misawa S, Kumagai I

Human inhibitory receptors Ig-like transcript 2 (ILT2) and ILT4 compete with CD8 for MHC class I binding and bind preferentially to HLA-G.

Shiroishi M, Tsumoto K, Amano K, Shirakihara Y, Colonna M, Braud VM, Allan DS, Makadzange A, Rowland-Jones S, Willcox B, Jones EY, van der Merwe PA, Kumagai I, Maenaka K

Structural consequences of target epitope-directed functional alteration of an antibody. The case of anti-hen lysozyme antibody, HyHEL-10.

Kumagai I, Nishimiya Y, Kondo H, Tsumoto K

The effects of arginine on refolding of aggregated proteins: not facilitate refolding, but suppress aggregation.

Arakawa T, Tsumoto K

Bypassing antibiotic selection: positive screening of genetically modified cells with an antigen-dependent proliferation switch.

Kawahara M, Ueda H, Morita S, Tsumoto K, Kumagai I, Nagamune T

Practical considerations in refolding proteins from inclusion bodies.

Tsumoto K, Ejima D, Kumagai I, Arakawa T

A morphologic study of filamentous phage infection of Escherichia coli using biotinylated phages.

Nakamura M, Tsumoto K, Kumagai I, Ishimura K

How additives influence the refolding of immunoglobulin-folded proteins in a stepwise dialysis system. Spectroscopic evidence for highly efficient refolding of a single-chain Fv fragment.

Umetsu M, Tsumoto K, Hara M, Ashish K, Goda S, Adschiri T, Kumagai I

Efficient construction of a diabody using a refolding system: anti-carcinoembryonic antigen recombinant antibody fragment.

Asano R, Kudo T, Nishimura Y, Makabe K, Hayashi H, Suzuki M, Tsumoto K, Kumagai I

The role of hydrogen bonding via interfacial water molecules in antigen-antibody complexation. The HyHEL-10-HEL interaction.

Yokota A, Tsumoto K, Shiroishi M, Kondo H, Kumagai I

Antitumor activity of interleukin-21 prepared by novel refolding procedure from inclusion bodies expressed in Escherichia coli.

Asano R, Kudo T, Makabe K, Tsumoto K, Kumagai I

Inhibition of hepatitis C virus NS3 protease by peptides derived from complementarity-determining regions (CDRs) of the monoclonal antibody 8D4: tolerance of a CDR peptide to conformational changes of a target.

Tsumoto K, Misawa S, Ohba Y, Ueno T, Hayashi H, Kasai N, Watanabe H, Asano R, Kumagai I

Chiral recognition in the binding of helicenediamine to double strand DNA: interactions between low molecular weight helical compounds and a helical polymer.

Honzawa S, Okubo H, Anzai S, Yamaguchi M, Tsumoto K, Kumagai I

Selection of human antibody fragments on the basis of stabilization of the variable domain in the presence of target antigens.

Watanabe H, Tsumoto K, Asano R, Nishimiya Y, Kumagai I

The effect of an agglutogen on virus infection: biotinylated filamentous phages and avidin as a model.

Nakamura M, Tsumoto K, Ishimura K, Kumagai I

T-cell immunotherapy for human MK-1-expressing tumors using a fusion protein of the superantigen SEA and anti-MK-1 scFv antibody.

Ueno A, Arakawa F, Abe H, Matsumoto H, Kudo T, Asano R, Tsumoto K, Kumagai I, Kuroki M, Kuroki M

Detection of biotinylated proteins in polyacrylamide gels using an avidin-fluorescein conjugate.

Nakamura M, Tsumoto K, Ishimura K, Kumagai I

Phage library panning against cytosolic fraction of cells using quantitative dot blotting assay: application of selected VH to histochemistry.

Nakamura M, Tsumoto K, Ishimura K, Kumagai I

A mutated superantigen SEA D227A fusion diabody specific to MUC1 and CD3 in targeted cancer immunotherapy for bile duct carcinoma.

Takemura S, Kudo T, Asano R, Suzuki M, Tsumoto K, Sakurai N, Katayose Y, Kodama H, Yoshida H, Ebara S, Saeki H, Imai K, Matsuno S, Kumagai I

Mutated SEA-D227A-conjugated antibodies greatly enhance antitumor activity against MUC1-expressing bile duct carcinoma.

Kodama H, Suzuki M, Katayose Y, Shinoda M, Sakurai N, Takemura S, Yoshida H, Saeki H, Ichiyama M, Tsumoto K, Asano R, Kumagai I, Imai K, Hinoda Y, Matsuno S, Kudo T

A visualization method of filamentous phage infection and phage-derived proteins in Escherichia coli using biotinylated phages.

Nakamura M, Tsumoto K, Ishimura K, Kumagai I

Contribution of Thr29 to the thermodynamic stability of goat alpha-lactalbumin as determined by experimental and theoretical approaches.

Horii K, Saito M, Yoda T, Tsumoto K, Matsushima M, Kuwajima K, Kumagai I

[Refolding of proteins from inclusion bodies].

Tsumoto K, Kumagai I, Misawa S

A growth signal with an artificially induced erythropoietin receptor-gp130 cytoplasmic domain heterodimer.

Kawahara M, Ueda H, Tsumoto K, Kumagai I, Mahoney W, Nagamune T

Replacing factor-dependency with that for lysozyme: affordable culture of IL-6-dependent hybridoma by transfecting artificial cell surface receptor.

Kawahara M, Natsume A, Terada S, Kato K, Tsumoto K, Kumagai I, Miki M, Mahoney W, Ueda H, Nagamune T

Structural evidence for entropic contribution of salt bridge formation to a protein antigen-antibody interaction: the case of hen lysozyme-HyHEL-10 Fv complex.

Shiroishi M, Yokota A, Tsumoto K, Kondo H, Nishimiya Y, Horii K, Matsushima M, Ogasahara K, Yutani K, Kumagai I

Inhibition of the hepatitis C virus NS3 protease activity by Fv fragment of antibody 8D4.

Kasai N, Tsumoto K, Niwa S, Misawa S, Ueno T, Hayashi H, Kumagai I

Panning of a phage VH library using nitrocellulose membranes: application to selection of a human VH library.

Nakamura M, Watanabe H, Nishimiya Y, Tsumoto K, Ishimura K, Kumagai I

Demonstration of a homogeneous noncompetitive immunoassay based on bioluminescence resonance energy transfer.

Arai R, Nakagawa H, Tsumoto K, Mahoney W, Kumagai I, Ueda H, Nagamune T

Folding-unfolding of goat alpha-lactalbumin studied by stopped-flow circular dichroism and molecular dynamics simulations.

Yoda T, Saito M, Arai M, Horii K, Tsumoto K, Matsushima M, Kumagai I, Kuwajima K

Construction of a diabody (small recombinant bispecific antibody) using a refolding system.

Takemura S, Asano R, Tsumoto K, Ebara S, Sakurai N, Katayose Y, Kodama H, Yoshida H, Suzuki M, Imai K, Matsuno S, Kudo T, Kumagai I

Cell-growth control by monomeric antigen: the cell surface expression of lysozyme-specific Ig V-domains fused to truncated Epo receptor.

Ueda H, Kawahara M, Aburatani T, Tsumoto K, Todokoro K, Suzuki E, Nishimura H, Schueler PA, Winter G, Mahoney WC, Kumagai I, Nagamune T

Functional Fv fragment of an antibody specific for CD28: Fv-mediated co-stimulation of T cells.

Takemura S, Asano R, Tsumoto K, Arai T, Sakurai N, Kodama H, Yoshida H, Katayose Y, Suzuki M, Matsuno S, Kudo T, Kumagai I

Isolation and characterization of monoclonal antibodies that inhibit hepatitis C virus NS3 protease.

Ueno T, Misawa S, Ohba Y, Matsumoto M, Mizunuma M, Kasai N, Tsumoto K, Kumagai I, Hayashi H

Fluorolabeling of antibody variable domains with green fluorescent protein variants: application to an energy transfer-based homogeneous immunoassay.

Arai R, Ueda H, Tsumoto K, Mahoney WC, Kumagai I, Nagamune T

Thermodynamic consequences of grafting enhanced affinity toward the mutated antigen onto an antibody. The case of anti-lysozyme antibody, HyHEL-10.

Nishimiya Y, Tsumoto K, Shiroishi M, Yutani K, Kumagai I

Functional construction of the anti-mucin core protein (MUC1) antibody MUSE11 variable regions in a bacterial expression system.

Asano R, Takemura S, Tsumoto K, Sakurai N, Teramae A, Ebara S, Katayose Y, Shinoda M, Suzuki M, Imai K, Matsuno S, Kudo T, Kumagai I

Homogeneous noncompetitive immunoassay based on the energy transfer between fluorolabeled antibody variable domains (open sandwich fluoroimmunoassay).

Ueda H, Kubota K, Wang Y, Tsumoto K, Mahoney W, Kumagai I, Nagamune T

Crystal structure of anti-Hen egg white lysozyme antibody (HyHEL-10) Fv-antigen complex. Local structural changes in the protein antigen and water-mediated interactions of Fv-antigen and light chain-heavy chain interfaces.

Kondo H, Shiroishi M, Matsushima M, Tsumoto K, Kumagai I

[Antibody production by phage library].

Kumagai I, Tsumoto K

Open sandwich ELISA with V(H)-/V(L)-alkaline phosphatase fusion proteins.

Suzuki C, Ueda H, Tsumoto K, Mahoney WC, Kumagai I, Nagamune T

SEA-scFv as a bifunctional antibody: construction of a bacterial expression system and its functional analysis.

Sakurai N, Kudo T, Suzuki M, Tsumoto K, Takemura S, Kodama H, Ebara S, Teramae A, Katayose Y, Shinoda M, Kurokawa T, Hinoda Y, Imai K, Matsuno S, Kumagai I

Cell-free expression of two single-chain monoclonal antibodies against lysozyme: effect of domain arrangement on the expression.

Merk H, Stiege W, Tsumoto K, Kumagai I, Erdmann VA

Effect of the extra n-terminal methionine residue on the stability and folding of recombinant alpha-lactalbumin expressed in Escherichia coli.

Chaudhuri TK, Horii K, Yoda T, Arai M, Nagata S, Terada TP, Uchiyama H, Ikura T, Tsumoto K, Kataoka H, Matsushima M, Kuwajima K, Kumagai I

Highly efficient recovery of functional single-chain Fv fragments from inclusion bodies overexpressed in Escherichia coli by controlled introduction of oxidizing reagent--application to a human single-chain Fv fragment.

Tsumoto K, Shinoki K, Kondo H, Uchikawa M, Juji T, Kumagai I

Calorimetric study of mutant human lysozymes with partially introduced Ca2+ binding sites and its efficient refolding system from inclusion bodies.

Koshiba T, Tsumoto K, Masaki K, Kawano K, Nitta K, Kumagai I

Construction of mono- and bivalent human single-chain Fv fragments against the D antigen in the Rh blood group: multimerization effect on cell agglutination and application to blood typing.

Furuta M, Uchikawa M, Ueda Y, Yabe T, Taima T, Tsumoto K, Kojima S, Juji T, Kumagai I

Novel selection method for engineered antibodies using the mechanism of Fv fragment stabilization in the presence of antigen.

Tsumoto K, Nishimiya Y, Kasai N, Ueda H, Nagamune T, Ogasahara K, Yutani K, Tokuhisa K, Matsushima M, Kumagai I

[Generation of novel functional antibody molecules by in vitro selection system].

Kumagai I, Tsumoto K

Role of salt bridge formation in antigen-antibody interaction. Entropic contribution to the complex between hen egg white lysozyme and its monoclonal antibody HyHEL10.

Tsumoto K, Ogasahara K, Ueda Y, Watanabe K, Yutani K, Kumagai I

Open sandwich ELISA: a novel immunoassay based on the interchain interaction of antibody variable region.

Ueda H, Tsumoto K, Kubota K, Suzuki E, Nagamune T, Nishimura H, Schueler PA, Winter G, Kumagai I, Mohoney WC

A stable phage-display system using a phagemid vector: phage display of hen egg-white lysozyme (HEL), Escherichia coli alkaline, phosphatase, and anti-HEL monoclonal antibody, HyHEL10.

Maenaka K, Furuta M, Tsumoto K, Watanabe K, Ueda Y, Kumagai I

Role of Tyr residues in the contact region of anti-lysozyme monoclonal antibody HyHEL10 for antigen binding.

Tsumoto K, Ogasahara K, Ueda Y, Watanabe K, Yutani K, Kumagai I

Contribution to antibody-antigen interaction of structurally perturbed antigenic residues upon antibody binding.

Tsumoto K, Ueda Y, Maenaka K, Watanabe K, Ogasahara K, Yutani K, Kumagai I

Effect of the order of antibody variable regions on the expression of the single-chain HyHEL10 Fv fragment in E. coli and the thermodynamic analysis of its antigen-binding properties.

Tsumoto K, Nakaoki Y, Ueda Y, Ogasahara K, Yutani K, Watanabe K, Kumagai I

Synthesis and expression of a DNA encoding the Fv domain of an anti-lysozyme monoclonal antibody, HyHEL10, in Streptomyces lividans.

Ueda Y, Tsumoto K, Watanabe K, Kumagai I

A protease inhibitor produced by Streptomyces lividans 66 exhibits inhibitory activities toward both subtilisin BPN' and trypsin.

Ueda Y, Kojima S, Tsumoto K, Takeda S, Miura K, Kumagai I