Research implementation status
In sub-theme I, patent application on several elemental technologies of the nanodiagnosis device developed so far was carried out toward the social implementation after the completion of the project. Moreover, we have developed a prototype of the cancer diagnostic device that integrates all the steps of time-consuming blood test on a single card, which comprise separation, purification and sensitive detection of miRNA in the serum. We evaluated the performance of the device using cell culture supernatants and miRNAs in the human blood, and obtained the prospect that the time required for the diagnosis can be greatly shortened to one hour from one day that was required by the conventional method.
In sub-theme 2, the mechanisms of Mn ion-loaded nanodevices to visualize the malignancy of tumors and detect microcarcinoma were demonstrated. For the targeted therapy of intractable cancers, the usefulness of polymeric micelles for the treatment of cancer stem cells was clarified, while the mechanisms of the micelles to target microcarcinoma were intensively investigated. Regarding nanodevices for the delivery of nucleic acids, their in vivo efficacy and safety were demonstrated, and these technologies were transferred to the company. Finally, polyion complex vesicles (PICsomes) were applied to the enzyme-prodrug therapy and their in vivo efficacy was successfully demonstrated.
In sub-theme 3, the photodynamic therapy (PDT) system utilizing nanodevices for the delivery of photosensitizers and homogeneous light irradiation devices was applied to spontaneous bladder cancer models in rats, which should be close to clinical cancers, and their efficacy and safety were confirmed. Regarding the sonodynamic therapy (SDT) utilizing nanodevices, the in vivo efficacy was demonstrated by using subcutaneous cancer models in mice and rabbits.
In the subtheme 4, aiming at the development of the implant device for reconstructing tissues, we achieved five substantial results: (1)the effect of bone and cartilage reconstructive factors on animal models, (2) the effect of mRNA delivery on the skeletal system, (3) the development of non-swellable and strong hydrogel composed of hydrophilic and temperature-sensitive polymers, (4) the effect of all-in-one titanium implant with mesh cage on the early bone regeneration and (5) the development of the deformable mesh plate conforming to complex 3D shapes.