Research implementation status
For the “Creation of Nano-Diagnosis System,” we investigated body fluid sample preparation and microRNA (miRNA) detection technology, using the miRNA purification and detection techniques and samples supplied by Takahiro Ochiya, National Cancer Center Research Institute. In the research for preperation, we developed techniques for single nanovesicle measurement and exosome isolation using microfluidic devices, whereas for detection, we succeeded in the identification of miRNA by non-bridging agglutination using nanoparticles, and quantification of 3-pM to 1-nM miR-141 by optical detection using microarrays. We accomplished hybridization detection using a 10-channel electrode array for electrometry.
For the “Creation of Nano-Drug Delivery System (Nano-DDS),” we demonstrated the concept of an integrated system of diagnosis and treatment for tracking the treatment effect of nano devices for accurate cancer diagnosis by diagnostic imaging. We also conducted non-clinical studies for additional indications for a nano device containing anticancer agents, optimizing the size of the device for intractable cancer treatment and increasing its functions (i.e., installation of ligand molecules, providing pH responsiveness). Furthermore, we conducted molecular modification and optimization of constituent macromolecules of nano devices containing small interfering RNAs (siRNAs) and hollow-form nano devices, and demonstrated in vivo efficacy for some of them.
For the “Creation of a Minimally Invasive Nano-Treatment System,” we conducted the molecular modification and optimization of constituent macromolecules of a nano device containing photosensitizers for application to the precise navigation treatment of superficial lesions with the nano device. Finally, we demonstrated in vivo efficacy of micelles containing photoresponsive anticancer agents. On the other hand, to facilitate the precise navigation treatment of deeply-placed lesions with the nano device, we developed a nano device containing photosensitizers and investigated the circuit of an ultrasonic irradiation system.
For the “Creation of Nano Reconstruction System,” we investigated a method for placing Nano-Drug Delivery System (DDS) in the device and their interactions to create tissue-inducible implant devices, which can control the sustained-release of nano DDS. Based on the results, we produced trial products, verified the production procedure for devices actually implanted, and established an animal model for evaluation of the devices, at the same time identifying several low-molecular compounds that strongly induce bone/cartilage differentiation, based on the examination of signaling factors that will be installed on the Nano-DDS.
“Social Deployment of Outcome” was the shared task among all sub-themes. To steadily implement the roadmap for practical use, we interviewed pharmaceutical regulatory affairs experts, and identified potential problems for products that will be developed in the future, from the perspectives of approval review and regulations.