An antigen-mediated selection system for mammalian cells that produce glycosylated single-chain Fv
Biochem. Biophys. Res. Commun., 324, 1165-1172 (2004).
DOI: 10.1016/j.bbrc.2004.09.178
PMID: 15504336
P. Pihkala, M. Kawahara*, H. Ueda, and T. Nagamune

Antigen-mediated genetically modified cell amplification (AMEGA) with single vector transduction
J. Chem. Eng. Jpn., 37, 1259-1264(2004).
DOI: 10.1252/jcej.37.1259
E. Kaneko, M. Kawahara*, K. Tsumoto, I. Kumagai, H. Ueda and T. Nagamune
Improved growth response of antibody/receptor chimera attained by the engineering of transmembrane domain
Protein Eng. Des. Sel., 17, 715-719(2004).
DOI: 10.1093/protein/gzh088
PMID: 15548567
M. Kawahara*, Y. Ogo, H. Ueda and T. Nagamune
Reversal of antigen-dependent signaling by two mutation in antibody/receptor chimera: implication of inverse agonism in cytokine receptor superfamily
Biochem. Pharmacol., 68, 539-548(2004).
DOI: 10.1016/j.bcp.2004.04.014
PMID: 15242820
M. Kawahara*, S. Ishii, K. Tsumoto, I. Kumagai, H. Ueda, and T. Nagamune

AMEGA: antigen-mediated genetically modified cell amplification
J. Immunol. Methods, 284, 187-194(2004).
DOI: 10.1016/j.jim.2003.10.007
PMID: 14736429
M. Kawahara*, H. Ueda, K. Tsumoto, I. Kumagai, and T. Nagamune

Selection of genetically modified cell population using hapten-specific antibody/receptor chimera
Biochem. Biophys. Res. Commun., 315, 132-138(2004).
DOI: 10.1016/j.bbrc.2004.01.030
PMID: 15013436
M. Kawahara*, H. Kimura, H. Ueda* and T. Nagamune

Peptidyl Linkers for Protein Heterodimerization Catalyzed by Microbial Transglutaminase
Bioconjugate Chem., 15, 491-497(2004).
DOI: 10.1021/bc034209o
PMID: 15149176
T. Tanaka, N. Kamiya and T. Nagamune*

Solid-phase artificial chaperone-assisted refolding using insoluble b-cyclodextrin-acrylamide copolymer beads
Biotechnol. Lett., 26, 1787-1791(2004).
DOI: 10.1007/s10529-004-4609-6
PMID: 15672215
S. Yamaguchi, C. Hong, T. Mannen, S. Tsukiji and T. Nagamune*
Screening of FGF target genes in Xenopus by microarray: temporal dissection of the signalling pathway using a chemical inhibitor
Genes Cells, 9, 749-761(2004).
DOI: 10.1111/j.1356-9597.2004.00761.x
PMID: 15298682
H. A. Chung, J. Hyodo-Miura, A. Kitayama, C. Terasaka, T. Nagamune and N. Ueno*

Conformation of Variably Linked Chimeric Proteins Evaluated by Synchrotron Small-Angle X-Ray Scattering
Proteins: Struct., Funct., Bioinf., 57, 829-838(2004).
DOI: 10.1002/prot.20244
PMID: 15390267
R. Arai, W. Wriggers, Y. Nishikawa, T. Nagamune and T. Fujisawa*
Rapid Protein Anchoring into the Membranes of Mammalian Cells Using Oleyl Chain and Poly(ethylene glycol) Derivertives
Biotechnol. Prog., 20, 897-904(2004).
DOI: 10.1021/bp0342093
PMID: 15176897
K. Kato, C. Itoh, T. Yasukouchi and T. Nagamune*

Transfection Microarray of Nonadherent Cells on an Oleyl Poly(ethylene glycol) ether-ModifiedGlass Slide
BioTechniques, 37, 444-452(2004).
DOI: 10.2144/04373RR02
PMID: 15470899
K. Kato, K. Umezawa, M. Miyake, J. Miyake and T. Nagamune*
Properties of an Alcohol dehydrogenase from the Hyperthermophilic Archaeon Aeropyrum pernix K1
J. Biosci. Bioeng., 97, 202-206(2004).
DOI: 10.1016/S1389-1723(04)70191-7
PMID: 16233615
H. Hirakawa, N. Kamiya, Y. Kawarabayashi and T. Nagamune*
Expression of amoA mRNA in wastewater treatment processes examined by competitive RT-PCR
J. Biotechnol., 111, 111-120(2004).
DOI: 10.1016/j.jbiotec.2004.02.017
PMID: 15219398
Y. Aoi*, Y. Shiramasa, Y. Masaki, S. Tsuneda, A. Hirata, A. Kitayama and T. Nagamune
Homogeneous sandwich immunoassay based on the enzymatic complementation induced by single-chain Fv fragments
Anal. Biochem., 327, 241-246(2004).
DOI: 10.1016/j.ab.2004.01.035
PMID: 15051541
N. Komiya, H. Ueda, Y. Ohiro and T. Nagamune*

Rectified photocurrent in a protein based molecular photo-diode consisting of a cytochromeb562-green fluorescent protein chimera self-assembled monolayer
Biosens. Bioelectron., 19, 1169-1174(2004).
DOI: 10.1016/j.bios.2003.11.016
PMID: 15046747
B. Lee, S. Takeda, K. Nakajima, J. Noh, JW. Choi, M. Hara and T. Nagamune