Using the reconstituted translation systems (PURE systems) originated of E. coli, yeast and mitochondria, we are promoting biochemical studies to elucidate more precise mechanism of translation process. Recently, yeast PURE system was successfully reconstituted and is now clarifying various regulation mechanism in translational process, such as mRNA-quality control. Studies on animal mitochondrial translation system are also in progress, which will provide us useful clues for the therapy of mitochondrial disease.
We are exploring protein-binders including
antibodies by the ribosome-display method based on the
PURE system. The peptide-drugs targeting GPCR synthesized by the
PURE system will be developed by the in vitro evolution system.
PURE ribosome display method