| Clone number | |||
| Clone name (database links) |
(EST database)(NEXTDB) (Sanger Centre) |
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| cDNA group
(database links) |
(EST database)(NEXTDB) |
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| Cosmid or YAC |
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| CDS identification number | |||
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database links
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BLAST search results
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| Homologues or conserved domains |
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| No. of C. elegans paralogues (E<1e-50) |
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| Differential hybridization |
high-density grid |
F cDNA probe |
R cDNA probe |
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| RNAi phenotype |
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embryonic viability (of F1)
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sterility (laid no eggs)
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sterility (laid more than 5 unfertilized
eggs)
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| clone number | 9 |
| clone name | yk385e11 |
| clone family | CELK 02567 |
| gene name | T03F1.5 |
| homology | S/T protein phosphatase PP1 |
| motif | - |
| in situ hybridization | L4 germline |
| RNAi | Spermatogenesis defects |
in situ hybridization:Staining
was observed during L4 in hermaphrodite and at all stage in male, when
the spermatogenesis occured.
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RNAi:Hermaphrodites did not have
sperm and laid eggs with apperarance similar to un fertilized eggs. Males
did not show any defects.
| No. of unfertilized eggs | No. of eggs | No. of hatched eggs | Hatching rate (%) |
| 0 | 85 | 76 | 89.4 |
| 0 | 75 | 69 | 92.0 |
| 0 | 68 | 60 | 88.2 |
| 0 | 92 | 77 | 83.7 |
| No. of unfertilized eggs | No. of eggs | No. of hatched eggs | Hatching rate (%) |
| 10 | 47 | 14 | 37.8 |
| 11 | 44 | 8 | 24.2 |
| 13 | 37 | 3 | 12.5 |
| 9 | 27 | 1 | 5.6 |
| 8 | 43 | 3 | 8.6 |
| 10 | 43 | 5 | 15.2 |
| 10 | 34 | 5 | 20.8 |
| 3 | 26 | 0 | 0 |
| 11 | 27 | 6 | 37.5 |
| 13 | 42 | 0 | 0 |
| 1 | 29 | 0 | 0 |
| 16 | 31 | 0 | 0 |
| 10 | 32 | 3 | 13.6 |
| 15 | 34 | 1 | 5.3 |
Photos
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