clone 50

Clone number
clone 50
Clone name
 (database links)
yk286h12
(EST database) (NEXTDB) (Sanger Centre)
cDNA group
 (database links)
CELK05366
(EST database) (NEXTDB)
Cosmid or YAC
C07H6
CDS identification number
x
Differential hybridization
Spot address on
high-density grid
Signal intensity for
F cDNA probe
Signal intensity for
R cDNA probe
006D02
51.999
0.619
RNAi phenotype
Sterile, Burst
embryonic viability (of F1)
80.3 %
sterility (laid no eggs)
60% (3 out of 5 F1 animals)
sterility (laid more than 5 unfertilized eggs)
0% (0 out of 5 F1 animals)
clone number 50
clone name yk286h12
clone family CELK 05366
gene name sequence in progress
homology novel
motif 
in situ hybridization
RNAi Glp phenotype and bursting

in situ hybridization:

RNAi:
P0 worms laid some unfertilized eggs.
About half of the F1 worms were dead because of bursting. The germline was also projected.
Many F1 worms were sterile.
 
Data (P0)
No. of unfertilized eggs No. of eggs No. of hatched eggs Hatching rate (%)
15 24 3 33.3
38 63 9 36
8 15 5 71.4
21 48 4 14.8
33 59 5 19.2
10 20 10 100
33 56 11 47.8
23 31 0 0
35 36 0 0
Data (F1)
No. of unfertilized eggs No. of eggs No. of hatched eggs Hatching rate (%)
0 0 0 -
0 23 1 4.3
0 14 0 0
0 0 0 -
0 18 5 27.8
0 7 0 0
0 13 3 23.1
0 0 0 -

Photos

Click on a picture to see a larger version.

 P0
after cross