clone 47

Clone number
clone 47
Clone name
 (database links)
yk270c1
(EST database) (NEXTDB) (Sanger Centre)
cDNA group
 (database links)
CELK01549
(EST database) (NEXTDB)
Cosmid or YAC
T25G3
CDS identification number
T25G3.2
database links
(Wormpep)
(WormBase)
(Worm PD)
BLAST search results
BLASTP against nr-aa
TBLASTN against human EST
TBLASTN against mouse EST
Homologues or conserved domains
Weak similarity to Yeast chitin synthase
No. of C. elegans paralogues (E<1e-50)
2
Differential hybridization
Spot address on
high-density grid
Signal intensity for
F cDNA probe
Signal intensity for
R cDNA probe
006C23
3.089
-
RNAi phenotype
Sterile
embryonic viability (of F1)
100.0 %
sterility (laid no eggs)
83.3% (5 out of 6 F1 animals)
sterility (laid more than 5 unfertilized eggs)
50% (3 out of 6 F1 animals)
clone number 47
clone name yk270c1
clone family CELK 01549
gene name T25G3.2
homology chitin synthase
motif integral membrane protein signal
in situ hybridization
RNAi Laid unfertilized eggs

in situ hybridization:

RNAi:  Laid unfertilized eggs
 
Exp. 1 (P0)
No. of unfertilized eggs No. of eggs No. of hatched eggs Hatching rate (%)
many 1
many 1
many 0
many 1
53 55 2 100
0 2 2 100
Exp. 1 (F1)
No. of unfertilized eggs No. of eggs No. of hatched eggs Hatching rate (%)
many 0
many 0
many 0
many 0
many 0
0 62 50 80.6
Exp. 2 (P0)
No. of unfertilized eggs No. of eggs No. of hatched eggs Hatching rate (%)
many 0
many 0
many 0
many 0

Photos

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F1
after cross
after cross
Spermatheca