clone 148

Clone number
clone 148
Clone name
 (database links)
yk574e1
(EST database) (NEXTDB) (Sanger Centre)
cDNA group
 (database links)
CELK00010
(EST database) (NEXTDB)
Cosmid or YAC
H02I12
CDS identification number
H02I12.1
database links
(Wormpep)
(WormBase)
(Worm PD)
BLAST search results
BLASTP against nr-aa
TBLASTN against human EST
TBLASTN against mouse EST
Homologues or conserved domains
-
No. of C. elegans paralogues (E<1e-50)
1
Differential hybridization
Spot address on
high-density grid
Signal intensity for
F cDNA probe
Signal intensity for
R cDNA probe
017H14
13.557
0.772
RNAi phenotype
Sterile
embryonic viability (of F1)
- %
sterility (laid no eggs)
50% (4 out of 8 F1 animals)
sterility (laid more than 5 unfertilized eggs)
50% (4 out of 8 F1 animals)
clone number 148
clone name yk574e1
clone family CELK 00010
gene name H02I12.1
homology -
motif  -
in situ hybridization
RNAi Laid unfertilized eggs

in situ hybridization:

RNAi:   Laid unfertilized eggs
 
Exp. 1 (P0)
No. of unfertilized eggs No. of eggs No. of hatchied eggs Hatching rate (%)
0 11
22 30
4 8
9 15
10 22
4 16
0 14
Exp. 1 (F1)
No. of unfertilized eggs No. of eggs No. of hatchied eggs Hatching rate (%)
9 11 2 100
0 0 0 -
0 1 0 0
3 3 0 -
4 8 2 50
0 0 0 -
0 0 0 -
11 17 4 66.7
The last worm in this table laid many fertilized eggs after cross with wild-type male.
 
Exp. 2 (F1)
No. of unfertilized eggs No. of eggs No. of hatchied eggs Hatching ra (%)
1 56 39 70.9
1 17 3 18.8
12 37 22 88
3 32 24 82.8
2 31 8 27.6
0 37 21 56.8
10 22 12 100
many
many
many

Photos :

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