clone 134

Clone number	clone 134
Clone name (database links)	yk540f8 (EST database) (NEXTDB) (Sanger Centre)
cDNA group (database links)	CELK04176 (EST database) (NEXTDB)
Cosmid or YAC	Y73B6BL
CDS identification number	x
Differential hybridization	Spot address on high-density grid	Signal intensity for F cDNA probe	Signal intensity for R cDNA probe
Differential hybridization	015J16	7.098	0.78
RNAi phenotype	Sterile, Embryonic lethal
embryonic viability (of F1)	6.8 %
sterility (laid no eggs)	28.5% (2 out of 7 F1 animals)
sterility (laid more than 5 unfertilized eggs)	0% (0 out of 7 F1 animals)

clone number	134
clone name	yk540f8
clone family	CELK 04176
gene name	-
homology	pumilio repeat family
motif	pumilio-family RNA binding domain
in situ hybridization
RNAi	Mog (Musculinization of the germline) Abnormal diakinesis Embryonic lethal

in situ hybridization:

RNAi: Mog (Musculinization of the germline)
Laid unfertilized eggs (P0)

Data (P0)
No. of unfertilized eggs No. of eggs No. of hatched eggs Hatching rate (%)

24 38 7 50

19 42 16 69.6

20 31 5 45.5

31 48 12 70.6

0 24 15 62.5

22 47 18 72

Data (F1)
No. of unfertilized eggs No. of eggs No. of hatched eggs Hatching rate (%)

1 7 1 16.7

0 0 0 -

0 0 0 -

4 18 0 0

0 28 27 96.4

3 22 6 31.6

0 34 31 91.2

Data (P0)
No. of unfertilized eggs	No. of eggs	No. of hatched eggs	Hatching rate (%)
24	38	7	50
19	42	16	69.6
20	31	5	45.5
31	48	12	70.6
0	24	15	62.5
22	47	18	72

Data (F1)
No. of unfertilized eggs	No. of eggs	No. of hatched eggs	Hatching rate (%)
1	7	1	16.7
0	0	0	-
0	0	0	-
4	18	0	0
0	28	27	96.4
3	22	6	31.6
0	34	31	91.2

Photos