clone 10

in situ hybridizationª@:ª@Staining was observed during L4 in hermaphrodite and at all stage in male, when the spermatogenesis occured.
 
 
 
 

RNAiª@:ª@Hermaphrodites did not have sperm and laid eggs with apperarance similar to un fertilized eggs. Males did not show any defects.

Ʀ°fª[°^
 

N2 ­ƒ°C°ã°W°F°N°g(F1)

ö¢Ú—Æü÷Î	ÚY÷ÎÆä	ýzÅÈÆä	ýzÅÈ÷Ϫ@(%)
7	7	0	-
16	16	0	-
31	31	0	-
0	29	28	96.6

cross­íÔ‹

ö¢Ú—Æü÷Î	ÚY÷ÎÆä	ýzÅÈÆä	ýzÅÈ÷Ϫ@(%)
9	91	81	98.8
25	114	89	100
13	83	60	85.7
			94.8ª}7.9

let-60::GFP (backcross­é­µ) ­ƒ°C°ã°W°F°N°g(F1)

ö¢Ú—Æü÷Î	ÚY÷ÎÆä	ýzÅÈÆä	ýzÅÈ÷Ϫ@(%)
4	4	0	-
11	15	0	0
23	25	0	0
17	28	0	0
7	7	0	-
16	22	0	0
1	6	1	20
11	30	0	0

let-60::GFP(backcross 
 X 4)­ƒ°C°ã°W°F°N°g(F1)

ö¢Ú—Æü÷Î	ÚY÷ÎÆä	ýzÅÈÆä	ýzÅÈ÷Ϫ@(%)
0	37	37	100
26	27	0	0
2	21	13	68.4
8	26	18	100
9	21	3	25
10	21	7	63.6
0	21	17	81
8	26	12	66.7
			72.1ª}25.7

 

Ù€ÉmäF

ö¢Ú—Æü÷Î	ÚY÷ÎÆä	ýzÅÈÆä	ýzÅÈ÷Ï
θ­¢
θ­¢
θ­¢
θ­¢
θ­¢
θ­¢
θ­¢
θ­¢
θ­¢
θ­¢
4	33	19	65.5
8	27	0	0
18	39	19	90.5
12	28	8	50
7	26	16	84.2

cross­íÔ‹

ö¢Ú—Æü÷Î	ÚY÷ÎÆä	ýzÅÈÆä	ýzÅÈ÷Ï
2	40
18	104
6	101

Photos

	before cross with N2 male	after cross with N2 male