Score E
Sequences producing significant alignments: (bits) Value
gb|AI346109.1|AI346109 qp48f03.x1 NCI_CGAP_Co8 Homo sapiens... 154 2e-36
gb|AI198525.1|AI198525 qf49e02.x1 Soares_testis_NHT Homo sa... 150 3e-35
gb|AW410521.1|AW410521 fh06g08.x1 NIH_MGC_17 Homo sapiens c... 148 9e-35
gb|AW085448.1|AW085448 wy65c03.x1 Soares_NSF_F8_9W_OT_PA_P_... 146 5e-34
gb|AI031678.1|AI031678 ow48g07.x1 Soares_parathyroid_tumor_... 143 3e-33
gb|AW949673.1|AW949673 EST361743 MAGE resequences, MAGA Hom... 140 3e-32
gb|AW239335.1|AW239335 xb39c04.y1 NCI_CGAP_Lu31 Homo sapien... 138 2e-31
gb|AW025978.1|AW025978 wv05a08.x1 NCI_CGAP_Kid3 Homo sapien... 136 7e-31
gb|AW250521.1|AW250521 2821580.5prime NIH_MGC_7 Homo sapien... 132 8e-30
gb|AW162829.1|AW162829 au87f11.y1 Schneider fetal brain 000... 132 1e-29
gb|AI802061.1|AI802061 tx29h01.x1 NCI_CGAP_Lu24 Homo sapien... 131 1e-29
gb|AI955196.1|AI955196 wx31b04.x1 NCI_CGAP_Pit1 Homo sapien... 131 2e-29
gb|AI879060.1|AI879060 au53h10.y1 Schneider fetal brain 000... 131 2e-29
gb|AA290668.1|AA290668 zt19e03.s1 Soares ovary tumor NbHOT ... 130 3e-29
gb|AW947938.1|AW947938 PM0-MT0011-240300-001-g04 MT0011 Hom... 129 5e-29
gb|AW027218.1|AW027218 wt72h05.x1 Soares_thymus_NHFTh Homo ... 129 5e-29
gb|AA429419.1|AA429419 zw51f01.r1 Soares_total_fetus_Nb2HF8... 129 7e-29
gb|AI815492.1|AI815492 au47h07.y1 Schneider fetal brain 000... 129 7e-29
gb|AW249697.1|AW249697 2819640.3prime NIH_MGC_7 Homo sapien... 128 1e-28
gb|AI755111.1|AI755111 cr35f02.x1 Jia bone marrow stroma Ho... 128 1e-28
gb|AW163681.1|AW163681 au97a05.y1 Schneider fetal brain 000... 128 1e-28
gb|AW732546.1|AW732546 bb08a09.y1 NIH_MGC_14 Homo sapiens c... 128 1e-28
gb|AW245833.1|AW245833 2822939.5prime NIH_MGC_7 Homo sapien... 128 1e-28
gb|AW238935.1|AW238935 xb34c12.y1 NCI_CGAP_Lu31 Homo sapien... 127 3e-28
gb|AW160662.1|AW160662 au74a11.y1 Schneider fetal brain 000... 127 3e-28
gb|AW615486.1|AW615486 ba09b11.x1 NIH_MGC_7 Homo sapiens cD... 126 4e-28
gb|W23213.1|W23213 80E4 Human retina cDNA Tsp509I-cleaved s... 126 4e-28
gb|AW732084.1|AW732084 ba07c08.x1 NIH_MGC_7 Homo sapiens cD... 126 6e-28
gb|AI492282.1|AI492282 tf59d10.x1 NCI_CGAP_Brn23 Homo sapie... 126 6e-28
gb|AA421521.1|AA421521 zu26d07.s1 Soares ovary tumor NbHOT ... 126 6e-28
gb|AW328657.1|AW328657 ds04a11.x1 NIH_MGC_4 Homo sapiens cD... 125 8e-28
gb|AA456986.1|AA456986 aa90a05.r1 Stratagene fetal retina 9... 125 8e-28
gb|AA398738.1|AA398738 zt75f03.s1 Soares_testis_NHT Homo sa... 125 1e-27
gb|AA312706.1|AA312706 EST183377 Jurkat T-cells VI Homo sap... 124 2e-27
gb|AW401497.1|AW401497 UI-HF-BK0-aau-b-05-0-UI.r1 NIH_MGC_3... 124 2e-27
gb|AW250789.1|AW250789 2822297.3prime NIH_MGC_7 Homo sapien... 123 3e-27
gb|AA526086.1|AA526086 ni58e02.s1 NCI_CGAP_Ov2 Homo sapiens... 123 4e-27
gb|BE207266.1|BE207266 ba15a05.y1 NIH_MGC_7 Homo sapiens cD... 123 4e-27
gb|AW247572.1|AW247572 2819517.5prime NIH_MGC_7 Homo sapien... 123 5e-27
gb|AW249048.1|AW249048 2821004.5prime NIH_MGC_7 Homo sapien... 122 7e-27
gb|AA600731.1|AA600731 np43f08.s1 NCI_CGAP_Br1.1 Homo sapie... 122 9e-27
gb|AW247546.1|AW247546 2819279.5prime NIH_MGC_7 Homo sapien... 122 1e-26
gb|AA613489.1|AA613489 nq42d07.s1 NCI_CGAP_Co10 Homo sapien... 120 3e-26
gb|AW249209.1|AW249209 2821029.5prime NIH_MGC_7 Homo sapien... 118 2e-25
gb|AA936907.1|AA936907 om55a05.s1 NCI_CGAP_GC4 Homo sapiens... 118 2e-25
gb|AA131453.1|AA131453 zo08a05.r1 Stratagene neuroepitheliu... 116 4e-25
gb|BE208250.1|BE208250 bb64g05.x1 NIH_MGC_9 Homo sapiens cD... 116 5e-25
gb|AW247828.1|AW247828 2820545.3prime NIH_MGC_7 Homo sapien... 116 5e-25
gb|AA506304.1|AA506304 nh45a06.s1 NCI_CGAP_Pr5 Homo sapiens... 116 6e-25
gb|AW406153.1|AW406153 UI-HF-BL0-aci-c-09-0-UI.r1 NIH_MGC_3... 115 1e-24
Alignments
>gb|AI346109.1|AI346109 qp48f03.x1 NCI_CGAP_Co8 Homo sapiens cDNA clone IMAGE:1926269 3'
similar to gb:D13748 EUKARYOTIC INITIATION FACTOR 4A-I
(HUMAN);.
Length = 896
Score = 154 bits (386), Expect = 2e-36
Identities = 91/227 (40%), Positives = 130/227 (57%), Gaps = 2/227 (0%)
Frame = -2
Query: 186 CKTLVLDEADKLLSQDFQGILDRLINFLPKERQVMLYSATFPNTVTSFMQKHMHKPYEIN 245
C+ L LD D+ S D L+ V+L S T P+ V + P+ I
Sbjct: 814 CEMLNLDSKDR--SMDISKRLNTTTG-------VVLLSTTMPSDVLEVTRSS*GTPFRIL 662
Query: 246 LME-ELTLLGVTQYYAFVQ-EKQKVHCLNTLFRKLQINQSIIFCNSTQRVELLAKKITEI 303
+ + ELTL G+ Q+Y V+ EK K+ L L+ L I Q++IF N ++V+ L +K+
Sbjct: 661 VKKKELTLXGIRQFYINVKREKWKLDTLCDLYETLTITQAVIFINXRRKVDWLTEKMHAR 482
Query: 304 GYSCYYIHSKMAQNHRNRVFHDFRQGNCRNLVCSDLLTRGIDIQAVNVVINFDFPRNAET 363
++ +H M Q R+ + +FR G+ R L+ +DLL RGID+Q V++VIN+D P N E
Sbjct: 481 DFTVSAMHGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNREN 302
Query: 364 YLHRIGRSGRFGHLGVAINLITYEDRHTLRRIEQELRTRIEPIPKTV 410
Y+HRIGR GRFG GVAIN++T ED+ TLR IE T IE +P V
Sbjct: 301 YIHRIGRGGRFGRKGVAINMVTEEDKRTLRDIETFYNTSIEEMPLNV 161
>gb|AI198525.1|AI198525 qf49e02.x1 Soares_testis_NHT Homo sapiens cDNA clone IMAGE:1753370
3' similar to gb:D13748 EUKARYOTIC INITIATION FACTOR
4A-I (HUMAN);.
Length = 824
Score = 150 bits (375), Expect = 3e-35
Identities = 81/186 (43%), Positives = 115/186 (61%), Gaps = 2/186 (1%)
Frame = -1
Query: 227 PNTVTSFMQKHMHKPYEINLM-EELTLLGVTQYYAFVQ-EKQKVHCLNTLFRKLQINQSI 284
P V +K M P I + EELTL G+ Q+Y V+ E+ K+ L L+ L I Q++
Sbjct: 716 PLIVLEVTKKFMRDPIRILVKKEELTLKGIRQFYINVEREEWKLDTLCDLYETLTITQAV 537
Query: 285 IFCNSTQRVELLAKKITEIGYSCYYIHSKMAQNHRNRVFHDFRQGNCRNLVCSDLLTRGI 344
IF N+ ++V+ L +K+ ++ +H M Q R+ + +FR G+ R L+ +DLL RGI
Sbjct: 536 IFINTRRKVDWLTEKMHARDFTVSAMHGDMDQKERDVIMREFRSGSSRVLITTDLLARGI 357
Query: 345 DIQAVNVVINFDFPRNAETYLHRIGRSGRFGHLGVAINLITYEDRHTLRRIEQELRTRIE 404
D+Q V++VIN+D P N E Y+HRIGR GRFG GVAIN++T ED+ TLR IE T IE
Sbjct: 356 DVQQVSLVINYDLPTNRENYIHRIGRGGRFGRKGVAINMVTEEDKRTLRDIETFYNTSIE 177
Query: 405 PIPKTV 410
+P V
Sbjct: 176 EMPLNV 159
>gb|AW410521.1|AW410521 fh06g08.x1 NIH_MGC_17 Homo sapiens cDNA clone IMAGE:2961591 5'.
Length = 637
Score = 148 bits (371), Expect = 9e-35
Identities = 80/183 (43%), Positives = 114/183 (61%), Gaps = 2/183 (1%)
Frame = +3
Query: 230 VTSFMQKHMHKPYEINLM-EELTLLGVTQYYAFVQ-EKQKVHCLNTLFRKLQINQSIIFC 287
V +K M P I + EELTL G+ Q+Y V+ E+ K+ L L+ L I Q++IF
Sbjct: 33 VLEVTKKFMRDPIRILVKKEELTLEGIRQFYINVEREEWKLDTLCDLYETLTITQAVIFI 212
Query: 288 NSTQRVELLAKKITEIGYSCYYIHSKMAQNHRNRVFHDFRQGNCRNLVCSDLLTRGIDIQ 347
N+ ++V+ L +K+ ++ +H M Q R+ + +FR G+ R L+ +DLL RGID+Q
Sbjct: 213 NTRRKVDWLTEKMHARDFTVSAMHGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQ 392
Query: 348 AVNVVINFDFPRNAETYLHRIGRSGRFGHLGVAINLITYEDRHTLRRIEQELRTRIEPIP 407
V++VIN+D P N E Y+HRIGR GRFG GVAIN++T ED+ TLR IE T IE +P
Sbjct: 393 QVSLVINYDLPTNRENYIHRIGRGGRFGRKGVAINMVTEEDKRTLRDIETFYNTSIEEMP 572
Query: 408 KTV 410
V
Sbjct: 573 LNV 581
>gb|AW085448.1|AW085448 wy65c03.x1 Soares_NSF_F8_9W_OT_PA_P_S1 Homo sapiens cDNA clone
IMAGE:2553412 3' similar to gb:D17532 PUTATIVE
ATP-DEPENDENT RNA HELICASE P54 (HUMAN);.
Length = 513
Score = 146 bits (365), Expect = 5e-34
Identities = 70/102 (68%), Positives = 83/102 (80%)
Frame = +2
Query: 329 GNCRNLVCSDLLTRGIDIQAVNVVINFDFPRNAETYLHRIGRSGRFGHLGVAINLITYED 388
G CRNLVC+DL TRGIDIQAVNVVINFDFP+NAETYLHRIGRSGR+GHLG+AINLIT ED
Sbjct: 8 GLCRNLVCTDLFTRGIDIQAVNVVINFDFPKNAETYLHRIGRSGRYGHLGLAINLITSED 187
Query: 389 RHTLRRIEQELRTRIEPIPKTVDPKLYVADQQLVDAADETTA 430
R L+ IE +L T I+PIP ++D LYVA+ ++ +E A
Sbjct: 188 RFNLKGIEDQLMTDIKPIPSSIDKSLYVAEFHSMNPDEEEAA 313
>gb|AI031678.1|AI031678 ow48g07.x1 Soares_parathyroid_tumor_NbHPA Homo sapiens cDNA clone
IMAGE:1650108 3' similar to gb:D13748 EUKARYOTIC
INITIATION FACTOR 4A-I (HUMAN);.
Length = 940
Score = 143 bits (358), Expect = 3e-33
Identities = 73/164 (44%), Positives = 106/164 (64%), Gaps = 1/164 (0%)
Frame = -2
Query: 248 EELTLLGVTQYYAFVQEKQ-KVHCLNTLFRKLQINQSIIFCNSTQRVELLAKKITEIGYS 306
EEL L G+ Q+Y V+ ++ K+ L L+ L I Q++IF N+ ++V+ L +K+ ++
Sbjct: 654 EELPLEGIRQFYINVKREE*KLDTLCDLYETLTITQAVIFINTRRKVDWLTEKMHARDFT 475
Query: 307 CYYIHSKMAQNHRNRVFHDFRQGNCRNLVCSDLLTRGIDIQAVNVVINFDFPRNAETYLH 366
+H M Q R+ + +FR G+ R L+ +DLL RGID+Q V++VIN+D P N E Y+H
Sbjct: 474 VSAMHGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIH 295
Query: 367 RIGRSGRFGHLGVAINLITYEDRHTLRRIEQELRTRIEPIPKTV 410
RIGR GRFG GVAIN++T ED+ TLR IE T IE +P V
Sbjct: 294 RIGRGGRFGRKGVAINMVTEEDKRTLRDIETFYNTSIEEMPLNV 163
>gb|AW949673.1|AW949673 EST361743 MAGE resequences, MAGA Homo sapiens cDNA.
Length = 702
Score = 140 bits (350), Expect = 3e-32
Identities = 71/201 (35%), Positives = 120/201 (59%), Gaps = 1/201 (0%)
Frame = +3
Query: 45 FEDFCLGRDLLMGIFEKGWEKPSPIQEASIGVALTGQDILARAKNGTGKTGAYCIPVIEK 104
F+D L LL GI+ G+EKPS IQ+ +I + G D++A+A++GTGKT + I ++++
Sbjct: 87 FDDMNLSESLLRGIYAYGFEKPSAIQQRAILPCIKGYDVIAQAQSGTGKTATFAISILQQ 266
Query: 105 IQPALKAIQAMVIVPTRELALQTSQICVELSKHIQLKVMVTTGGTDLRDDIMRLN-GTVH 163
I+ LKA QA+V+ PTRELA Q ++ + L ++ GGT++R ++ +L H
Sbjct: 267 IELDLKATQALVLAPTRELAQQIQKVVMALGDYMGASCHACIGGTNVRAEVQKLQMEAPH 446
Query: 164 LVIATPGRILDLMEKGVAKMEHCKTLVLDEADKLLSQDFQGILDRLINFLPKERQVMLYS 223
+++ TPGR+ D++ + ++ K VLDEAD++L++ F+ + + L QV+L
Sbjct: 447 IIVGTPGRVFDMLNRRYLSPKYIKMFVLDEADEMLNRGFKDQIYDIFQKLNSNTQVVLLV 626
Query: 224 ATFPNTVTSFMQKHMHKPYEI 244
AT P+ V ++ M P I
Sbjct: 627 ATMPSDVLEVTKEFMRDPIPI 689
>gb|AW239335.1|AW239335 xb39c04.y1 NCI_CGAP_Lu31 Homo sapiens cDNA clone IMAGE:2578662 5'
similar to gb:D13748 EUKARYOTIC INITIATION FACTOR 4A-I
(HUMAN);.
Length = 638
Score = 138 bits (343), Expect = 2e-31
Identities = 69/187 (36%), Positives = 115/187 (60%), Gaps = 1/187 (0%)
Frame = +3
Query: 45 FEDFCLGRDLLMGIFEKGWEKPSPIQEASIGVALTGQDILARAKNGTGKTGAYCIPVIEK 104
F+D L LL GI+ G+EKPS IQ+ +I + G D++A+A++GTGKT + I ++++
Sbjct: 72 FDDMNLSESLLRGIYAYGFEKPSAIQQRAILPCIKGYDVIAQAQSGTGKTATFAISILQQ 251
Query: 105 IQPALKAIQAMVIVPTRELALQTSQICVELSKHIQLKVMVTTGGTDLRDDIMRLN-GTVH 163
I+ LKA QA+V+ PTRELA Q ++ + L ++ GGT++R ++ +L H
Sbjct: 252 IELDLKATQALVLAPTRELAQQIQKVVMALGDYMGASCHACIGGTNVRAEVQKLQMEAPH 431
Query: 164 LVIATPGRILDLMEKGVAKMEHCKTLVLDEADKLLSQDFQGILDRLINFLPKERQVMLYS 223
+++ TPGR+ D++ + + K VLDEAD++LS+ F+ + + L + V+L S
Sbjct: 432 IIVGTPGRVXDMLNRRYLSPRYIKMFVLDEADEMLSRGFKDQIYDIFQKLKQHTHVVLLS 611
Query: 224 ATFPNTV 230
AT P+ V
Sbjct: 612 ATMPSDV 632
>gb|AW025978.1|AW025978 wv05a08.x1 NCI_CGAP_Kid3 Homo sapiens cDNA clone IMAGE:2528630 3'
similar to gb:D13748 EUKARYOTIC INITIATION FACTOR 4A-I
(HUMAN);.
Length = 671
Score = 136 bits (338), Expect = 7e-31
Identities = 68/160 (42%), Positives = 103/160 (63%), Gaps = 1/160 (0%)
Frame = -3
Query: 252 LLGVTQYYAFVQEKQ-KVHCLNTLFRKLQINQSIIFCNSTQRVELLAKKITEIGYSCYYI 310
L G+ ++Y V+ ++ K++ L L+ L I Q++IF N+ ++V+ L +K+ ++ +
Sbjct: 633 LEGIRKFYINVEREE*KLNTLCDLYETLTITQAVIFINTRRKVDWLTEKMHARDFTVSAM 454
Query: 311 HSKMAQNHRNRVFHDFRQGNCRNLVCSDLLTRGIDIQAVNVVINFDFPRNAETYLHRIGR 370
H M Q R+ + +FR G+ R L+ +DLL RGID+Q V++VIN+D P N E Y+HRIGR
Sbjct: 453 HGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIHRIGR 274
Query: 371 SGRFGHLGVAINLITYEDRHTLRRIEQELRTRIEPIPKTV 410
GRFG GVAIN++T ED+ TL IE T IE +P V
Sbjct: 273 GGRFGRKGVAINMVTEEDKRTLXDIETFYNTSIEEMPLNV 154
>gb|AW250521.1|AW250521 2821580.5prime NIH_MGC_7 Homo sapiens cDNA clone IMAGE:2821580 5'.
Length = 563
Score = 132 bits (329), Expect = 8e-30
Identities = 66/181 (36%), Positives = 111/181 (60%), Gaps = 1/181 (0%)
Frame = +1
Query: 62 GWEKPSPIQEASIGVALTGQDILARAKNGTGKTGAYCIPVIEKIQPALKAIQAMVIVPTR 121
G+EKPS IQ+ +I + G D++A+A++GTGKT + I ++++I+ LKA QA+V+ PTR
Sbjct: 13 GFEKPSAIQQRAILPCIKGYDVIAQAQSGTGKTATFAISILQQIELDLKATQALVLAPTR 192
Query: 122 ELALQTSQICVELSKHIQLKVMVTTGGTDLRDDIMRLN-GTVHLVIATPGRILDLMEKGV 180
ELA Q ++ + L ++ GGT++R ++ +L H+++ TPGR+ D++ +
Sbjct: 193 ELAQQIQKVVMALGDYMGASCHACIGGTNVRAEVQKLQMEAPHIIVGTPGRVFDMLNRRY 372
Query: 181 AKMEHCKTLVLDEADKLLSQDFQGILDRLINFLPKERQVMLYSATFPNTVTSFMQKHMHK 240
++ K VLDEAD++LS+ F+ + + L QV+L SAT P+ V +K M
Sbjct: 373 LSPKYIKMFVLDEADEMLSRGFKDQIYDIFQKLNSNTQVVLLSATMPSDVLEVTKKFMRD 552
Query: 241 P 241
P
Sbjct: 553 P 555
>gb|AW162829.1|AW162829 au87f11.y1 Schneider fetal brain 00004 Homo sapiens cDNA clone
IMAGE:2783277 5' similar to gb:D13748 EUKARYOTIC
INITIATION FACTOR 4A-I (HUMAN);.
Length = 650
Score = 132 bits (328), Expect = 1e-29
Identities = 66/182 (36%), Positives = 112/182 (61%), Gaps = 1/182 (0%)
Frame = +1
Query: 45 FEDFCLGRDLLMGIFEKGWEKPSPIQEASIGVALTGQDILARAKNGTGKTGAYCIPVIEK 104
F+D L LL GI+ G+EKPS IQ+ +I + G D++A+A++GTGKT + I ++++
Sbjct: 100 FDDMNLSESLLRGIYAYGFEKPSAIQQRAILPCIKGYDVIAQAQSGTGKTATFAISILQQ 279
Query: 105 IQPALKAIQAMVIVPTRELALQTSQICVELSKHIQLKVMVTTGGTDLRDDIMRLN-GTVH 163
I+ LKA QA+V+ PTRELA Q ++ + L ++ GGT++R ++ +L H
Sbjct: 280 IELDLKATQALVLAPTRELAQQIQKVVMALGDYMGASCHACIGGTNVRAEVQKLQMEAPH 459
Query: 164 LVIATPGRILDLMEKGVAKMEHCKTLVLDEADKLLSQDFQGILDRLINFLPKERQVMLYS 223
+++ TPGR+ D++ + ++ K VLDEAD++LS+ F+ + + L V++ S
Sbjct: 460 IIVGTPGRVFDMLNRRYLSPKYIKMFVLDEADEMLSRGFKDQIYDIFQKLNSNTPVVVLS 639
Query: 224 AT 225
AT
Sbjct: 640 AT 645
Database: GenBank Human EST entries
Posted date: Jul 9, 2000 8:11 PM
Number of letters in database: 800,131,619
Number of sequences in database: 2,121,173
Lambda K H
0.322 0.138 0.400
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 347992233
Number of Sequences: 2121173
Number of extensions: 4145995
Number of successful extensions: 20756
Number of sequences better than 10.0: 1906
Number of HSP's better than 10.0 without gapping: 1822
Number of HSP's successfully gapped in prelim test: 125
Number of HSP's that attempted gapping in prelim test: 18667
Number of HSP's gapped (non-prelim): 2016
length of query: 430
length of database: 266,710,539
effective HSP length: 56
effective length of query: 374
effective length of database: 147,924,851
effective search space: 55323894274
effective search space used: 55323894274
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.9 bits)
S2: 72 (32.5 bits)
