Score E
Sequences producing significant alignments: (bits) Value
emb|AL134915.1|AL134915 DKFZp762P1310_r1 762 (synonym: hmel... 277 2e-73
gb|AA657488.1|AA657488 nt66e08.s1 NCI_CGAP_Pr3 Homo sapiens... 187 5e-46
gb|AA293414.1|AA293414 zt53f11.r1 Soares ovary tumor NbHOT ... 156 9e-37
gb|AA314909.1|AA314909 EST186723 HCC cell line (matastasis ... 154 3e-36
gb|AA868697.1|AA868697 ak53g01.s1 Soares_testis_NHT Homo sa... 69 7e-34
gb|AI761822.1|AI761822 wi62g07.x1 NCI_CGAP_Co16 Homo sapien... 144 4e-33
gb|AA085567.1|AA085567 zn44f08.r1 Stratagene HeLa cell s3 9... 134 3e-30
gb|AI014916.1|AI014916 ot82b07.s1 Soares_total_fetus_Nb2HF8... 129 1e-28
gb|AA576496.1|AA576496 nm76b06.s1 NCI_CGAP_Co9 Homo sapiens... 124 4e-27
gb|AA027889.1|AA027889 zk05d01.r1 Soares_pregnant_uterus_Nb... 122 2e-26
gb|AW589985.1|AW589985 hg28d02.x1 NCI_CGAP_GC6 Homo sapiens... 122 2e-26
gb|AA330466.1|AA330466 EST34176 Embryo, 12 week II Homo sap... 122 2e-26
gb|AA223635.1|AA223635 zr07f03.r1 Stratagene NT2 neuronal p... 107 4e-22
gb|AW452099.1|AW452099 UI-H-BI3-alo-d-05-0-UI.s1 NCI_CGAP_S... 107 5e-22
gb|AI990211.1|AI990211 ws38f05.x1 NCI_CGAP_GC6 Homo sapiens... 107 5e-22
gb|R51715.1|R51715 yg77d07.r1 Soares infant brain 1NIB Homo... 106 7e-22
gb|AA382565.1|AA382565 EST95859 Testis I Homo sapiens cDNA ... 100 5e-20
gb|AI963910.1|AI963910 wt86f08.x1 NCI_CGAP_GC6 Homo sapiens... 99 2e-19
gb|AI968871.1|AI968871 wq67a10.x1 NCI_CGAP_GC6 Homo sapiens... 95 3e-18
gb|R61743.1|R61743 yh09c09.r1 Soares infant brain 1NIB Homo... 92 2e-17
gb|AW665977.1|AW665977 hj10e12.x1 Soares_NFL_T_GBC_S1 Homo ... 89 1e-16
gb|BE001731.1|BE001731 PM0-BN0082-140300-001-d01 BN0082 Hom... 85 3e-15
gb|AA868696.1|AA868696 ak53f12.s1 Soares_testis_NHT Homo sa... 61 4e-14
gb|H03156.1|H03156 yj44f02.r1 Soares placenta Nb2HP Homo sa... 53 6e-13
gb|AI962254.1|AI962254 wq46a09.x1 NCI_CGAP_GC6 Homo sapiens... 77 6e-13
gb|AA877271.1|AA877271 nq94e09.s1 NCI_CGAP_Co10 Homo sapien... 68 4e-10
gb|AW995311.1|AW995311 QV0-BN0042-170300-162-h10 BN0042 Hom... 59 1e-07
gb|AW294538.1|AW294538 UI-H-BI2-ahk-b-02-0-UI.s1 NCI_CGAP_S... 53 8e-06
gb|AI743478.1|AI743478 wf63f11.x2 Soares_NFL_T_GBC_S1 Homo ... 46 0.001
gb|AA304506.1|AA304506 EST17276 Aorta endothelial cells, TN... 45 0.002
gb|AW366567.1|AW366567 RC4-HT0147-131099-011-c03 HT0147 Hom... 43 0.012
gb|W23746.1|W23746 zb46e11.r1 Soares_fetal_lung_NbHL19W Hom... 42 0.027
Alignments
>emb|AL134915.1|AL134915 DKFZp762P1310_r1 762 (synonym: hmel2) Homo sapiens cDNA clone
DKFZp762P1310 5'.
Length = 609
Score = 277 bits (702), Expect = 2e-73
Identities = 130/201 (64%), Positives = 164/201 (80%)
Frame = -2
Query: 412 LIMVACGTSYHSAIACRQIXXXXXXXXXXXXXASDFLDRETPIFRDDVCLFISQSGETAD 471
LI++ACGTSYH+ +A RQ+ ASDFLDR TP+FRDDVC F+SQSGETAD
Sbjct: 608 LILIACGTSYHAGVATRQVLEELTELPVMVELASDFLDRNTPVFRDDVCFFLSQSGETAD 429
Query: 472 TLLALRYCKPRGALTIGVTNTVGSSICRESHCGIHINAGPEIGVASTKAYTSQILSLLMF 531
TL+ LRYCK RGALT+G+TNTVGSSI RE+ CG+HINAGPEIGVASTKAYTSQ +SL+MF
Sbjct: 428 TLMGLRYCKERGALTVGITNTVGSSISRETDCGVHINAGPEIGVASTKAYTSQFVSLVMF 249
Query: 532 ALTLSDDRISMAKRREEIIDALNDLPELIREVLQLDEKVLDIAKQIYKEKSLLIMGRGLN 591
AL + DDRISM +RR+EI+ L LP+LI+EVL +D+++ +A ++Y +KS+LIMGRG +
Sbjct: 248 ALMMCDDRISMQERRKEIMLGLKRLPDLIKEVLSMDDEIQKLATELYHQKSVLIMGRGYH 69
Query: 592 FATCLEGALKIKELSYMHCEG 612
+ATCLEGALKIKE+ Y+H +G
Sbjct: 68 YATCLEGALKIKEIXYIHQQG 6
>gb|AA657488.1|AA657488 nt66e08.s1 NCI_CGAP_Pr3 Homo sapiens cDNA clone IMAGE:1203494
similar to gb:M90516 GLUCOSAMINE--FRUCTOSE-6-PHOSPHATE
AMINOTRANSFERASE (HUMAN);.
Length = 497
Score = 187 bits (469), Expect = 5e-46
Identities = 90/164 (54%), Positives = 124/164 (74%)
Frame = -3
Query: 559 LIREVLQLDEKVLDIAKQIYKEKSLLIMGRGLNFATCLEGALKIKELSYMHCEGIMSGEL 618
LI VL + +++ +A ++ +KS+LIMGRG ++ATCLEGALKIKE++YMH EGI++GEL
Sbjct: 495 LIMTVLSMHDEIQKLATELXHQKSVLIMGRGYHYATCLEGALKIKEITYMHSEGILAGEL 316
Query: 619 KHGPLAMVDEFLSICMVVCNDRVYKKSLNALQQVVARKGAPIIIADCTVPEGDLAGMKHI 678
KHGPLA+VD+ + + M++ D Y K NALQQVVAR+G P++I D E + K
Sbjct: 315 KHGPLALVDKLMPVIMIIMRDHTYAKCQNALQQVVARQGRPVVICDKEDTE-TIKNTKRT 139
Query: 679 LRVPKTVDCVQNILTVIPLQLLSYHIAELNGANVDRPRNLAKSV 722
++VP +VDC+Q IL+VIPLQLL++H+A L G +VD PRNLAKSV
Sbjct: 138 IKVPHSVDCLQGILSVIPLQLLAFHLAVLRGYDVDFPRNLAKSV 7
>gb|AA293414.1|AA293414 zt53f11.r1 Soares ovary tumor NbHOT Homo sapiens cDNA clone
IMAGE:726093 5' similar to gb:M90516
GLUCOSAMINE--FRUCTOSE-6-PHOSPHATE AMINOTRANSFERASE
(HUMAN);.
Length = 369
Score = 156 bits (390), Expect = 9e-37
Identities = 77/103 (74%), Positives = 86/103 (82%)
Frame = +2
Query: 444 ASDFLDRETPIFRDDVCLFISQSGETADTLLALRYCKPRGALTIGVTNTVGSSICRESHC 503
ASDFLDR TP+FRDDVC FISQSGETADTLLALRYCK RGALT+GV NTVGSSI RE+ C
Sbjct: 44 ASDFLDRNTPVFRDDVCFFISQSGETADTLLALRYCKDRGALTVGVDNTVGSSISRETDC 223
Query: 504 GIHINAGPEIGVASTKAYTSQILSLLMFALTLSDDRISMAKRR 546
G+HINAGPEIGVA TKAYTSQ+ SL+M L + D+ S +R
Sbjct: 224 GVHINAGPEIGVAITKAYTSQVNSLVMVGLYV*DEFHSNRSKR 352
>gb|AA314909.1|AA314909 EST186723 HCC cell line (matastasis to liver in mouse) II Homo
sapiens cDNA 5' end similar to glutamine:fructose
6-phosphate amidotransferase.
Length = 422
Score = 154 bits (386), Expect = 3e-36
Identities = 76/135 (56%), Positives = 100/135 (73%)
Frame = +2
Query: 591 NFATCLEGALKIKELSYMHCEGIMSGELKHGPLAMVDEFLSICMVVCNDRVYKKSLNALQ 650
++ATC EGALKIKE++YMH EGI++G LKHGPLA+VD + + M++ D K ALQ
Sbjct: 5 HYATCXEGALKIKEITYMHSEGILAGXLKHGPLALVDXLMPVIMIIMRDXTXXKCQXALQ 184
Query: 651 QVVARKGAPIIIADCTVPEGDLAGMKHILRVPKTVDCVQNILTVIPLQLLSYHIAELNGA 710
QVVAR+G P++I D E + K ++VP +VDC+Q IL+VIPLQLL++H+A L G
Sbjct: 185 QVVARQGRPVVICDKEDTE-IIKNTKRTIKVPHSVDCLQGILSVIPLQLLAFHLAVLRGY 361
Query: 711 NVDRPRNLAKSVTVE 725
+VD PRNLAKSVTVE
Sbjct: 362 DVDFPRNLAKSVTVE 406
>gb|AA868697.1|AA868697 ak53g01.s1 Soares_testis_NHT Homo sapiens cDNA clone IMAGE:1409712
3' similar to gb:M90516
GLUCOSAMINE--FRUCTOSE-6-PHOSPHATE AMINOTRANSFERASE
(HUMAN);.
Length = 502
Score = 68.7 bits (165), Expect(3) = 7e-34
Identities = 32/46 (69%), Positives = 40/46 (86%)
Frame = -1
Query: 680 RVPKTVDCVQNILTVIPLQLLSYHIAELNGANVDRPRNLAKSVTVE 725
+VP +VDC+Q IL+VIPLQLL++H+A L G +VD PRNLAKSVTVE
Sbjct: 211 QVPHSVDCLQGILSVIPLQLLAFHLAVLRGYDVDSPRNLAKSVTVE 74
Score = 61.7 bits (147), Expect(3) = 7e-34
Identities = 26/48 (54%), Positives = 35/48 (72%)
Frame = -2
Query: 617 ELKHGPLAMVDEFLSICMVVCNDRVYKKSLNALQQVVARKGAPIIIAD 664
E KHGPLA+VD+ + + M++ D Y K NALQQVVAR+G P++I D
Sbjct: 396 EWKHGPLALVDKLMPVIMIIMRDHTYAKCQNALQQVVARQGRPVVICD 253
Score = 58.2 bits (138), Expect(3) = 7e-34
Identities = 25/34 (73%), Positives = 32/34 (93%)
Frame = -3
Query: 582 SLLIMGRGLNFATCLEGALKIKELSYMHCEGIMS 615
S+LIMGRG ++ATCLEGALKIKE++YMH EGI++
Sbjct: 500 SVLIMGRGYHYATCLEGALKIKEITYMHSEGILA 399
>gb|AI761822.1|AI761822 wi62g07.x1 NCI_CGAP_Co16 Homo sapiens cDNA clone IMAGE:2394876 3'
similar to gb:M90516 GLUCOSAMINE--FRUCTOSE-6-PHOSPHATE
AMINOTRANSFERASE (HUMAN);.
Length = 627
Score = 144 bits (359), Expect = 4e-33
Identities = 70/125 (56%), Positives = 93/125 (74%)
Frame = -2
Query: 601 KIKELSYMHCEGIMSGELKHGPLAMVDEFLSICMVVCNDRVYKKSLNALQQVVARKGAPI 660
+IK ++YMH EGI++GELKHGPLA VD+ + + M++ D Y K NALQQVVAR+G P+
Sbjct: 611 EIKXITYMHSEGILAGELKHGPLAXVDKLMPVIMIIMRDHTYAKCQNALQQVVARQGRPV 432
Query: 661 IIADCTVPEGDLAGMKHILRVPKTVDCVQNILTVIPLQLLSYHIAELNGANVDRPRNLAK 720
+I D E + K ++VP +VDC+Q IL+VIPLQLL++H+A L G +VD PRNLAK
Sbjct: 431 VICDKEDTE-TIKNTKRTIKVPHSVDCLQGILSVIPLQLLAFHLAVLRGYDVDFPRNLAK 255
Query: 721 SVTVE 725
SVTVE
Sbjct: 254 SVTVE 240
>gb|AA085567.1|AA085567 zn44f08.r1 Stratagene HeLa cell s3 937216 Homo sapiens cDNA clone
IMAGE:550311 5' similar to gb:M90516
GLUCOSAMINE--FRUCTOSE-6-PHOSPHATE AMINOTRANSFERASE
(HUMAN);.
Length = 356
Score = 134 bits (334), Expect = 3e-30
Identities = 63/85 (74%), Positives = 75/85 (88%)
Frame = +2
Query: 463 ISQSGETADTLLALRYCKPRGALTIGVTNTVGSSICRESHCGIHINAGPEIGVASTKAYT 522
+SQSGETADTL+ LRYCK RGALT+G+TNTVGSSI RE+ CG+HINAGPEIGVASTKAYT
Sbjct: 2 LSQSGETADTLMGLRYCKERGALTVGITNTVGSSISRETDCGVHINAGPEIGVASTKAYT 181
Query: 523 SQILSLLMFALTLSDDRISMAKRRE 547
SQ +SL+MFAL + DRISM ++ +
Sbjct: 182 SQFVSLVMFALMMCXDRISMQEKTQ 256
>gb|AI014916.1|AI014916 ot82b07.s1 Soares_total_fetus_Nb2HF8_9w Homo sapiens cDNA clone
IMAGE:1623253 3' similar to gb:M90516
GLUCOSAMINE--FRUCTOSE-6-PHOSPHATE AMINOTRANSFERASE
(HUMAN);.
Length = 541
Score = 129 bits (320), Expect = 1e-28
Identities = 61/121 (50%), Positives = 89/121 (73%), Gaps = 1/121 (0%)
Frame = -1
Query: 606 SYMHCEGIMSGELKHGPLAMVDEFLSICMVVCNDRVYKKSLNALQQVVARKGAPIIIADC 665
+Y+H EGI+ GE+ HGPLA++D+ + + M++ D Y + NALQQV+AR+G P++I C
Sbjct: 490 TYLHSEGILLGEMTHGPLALLDKLMPVIMIIMRDHTYAQCQNALQQVLARQGRPVVI--C 317
Query: 666 TVPEGD-LAGMKHILRVPKTVDCVQNILTVIPLQLLSYHIAELNGANVDRPRNLAKSVTV 724
+ + + K ++VP +VDC+Q IL+VIPLQLL++H+A L G +VD PRNLAKSVTV
Sbjct: 316 AKEDTETIKNTKRTIKVPHSVDCLQGILSVIPLQLLAFHLAVLRGYDVDFPRNLAKSVTV 137
Query: 725 E 725
E
Sbjct: 136 E 134
>gb|AA576496.1|AA576496 nm76b06.s1 NCI_CGAP_Co9 Homo sapiens cDNA clone IMAGE:1074131 3'
similar to gb:M90516 GLUCOSAMINE--FRUCTOSE-6-PHOSPHATE
AMINOTRANSFERASE (HUMAN);.
Length = 567
Score = 124 bits (308), Expect = 4e-27
Identities = 68/154 (44%), Positives = 97/154 (62%)
Frame = -1
Query: 572 DIAKQIYKEKSLLIMGRGLNFATCLEGALKIKELSYMHCEGIMSGELKHGPLAMVDEFLS 631
+ +++ + L MGR + TCL+GA+KI+ S +H E + EL+ P A+VD
Sbjct: 561 ETGNELFHKIPFLKMGRSYH*PTCLKGAIKIQGNSLLHSESNLGCELEPWPFALVD*LDP 382
Query: 632 ICMVVCNDRVYKKSLNALQQVVARKGAPIIIADCTVPEGDLAGMKHILRVPKTVDCVQNI 691
+ M++ D K NA QQV AR+G P++I D E + K ++VP +VDC+Q I
Sbjct: 381 VNMIILRDHT*AKCQNAPQQVGARQGRPVVICDKEDTE-TIKNTKRTIKVPHSVDCLQGI 205
Query: 692 LTVIPLQLLSYHIAELNGANVDRPRNLAKSVTVE 725
L+VIPLQLL++H+A L G +VD PRNLAKSVTVE
Sbjct: 204 LSVIPLQLLAFHLAVLRGYDVDFPRNLAKSVTVE 103
>gb|AA027889.1|AA027889 zk05d01.r1 Soares_pregnant_uterus_NbHPU Homo sapiens cDNA clone
IMAGE:469633 5' similar to gb:M90516
GLUCOSAMINE--FRUCTOSE-6-PHOSPHATE AMINOTRANSFERASE
(HUMAN);.
Length = 363
Score = 122 bits (302), Expect = 2e-26
Identities = 61/119 (51%), Positives = 85/119 (71%), Gaps = 2/119 (1%)
Frame = +1
Query: 588 RGLNFATCLEGALKIKELSYMHCEGIMSGELKHGPLAMVDEFLSICMVVCNDRVYKKSLN 647
RG ++ATCLEGALKIKE++YMH EGI++GELKHGPLA+VD+ + + M++ D Y K N
Sbjct: 1 RGYHYATCLEGALKIKEITYMHSEGILAGELKHGPLALVDKLMPVIMIIMRDHTYAKCQN 180
Query: 648 ALQQVVARKGAPIIIADCTVPEGDLAGMKHILRVPKTVDCVQN-ILTVIPL-QLLSYHI 704
ALQQVVAR+G P++I D E + K ++VP +VD + IL PL +LL++H+
Sbjct: 181 ALQQVVARQGRPVVICDXEDTE-TIKNTKRTIKVPHSVDLLAGAILXXXPLYRLLAFHL 354
Database: GenBank Human EST entries
Posted date: Jul 8, 2000 8:03 PM
Number of letters in database: 800,131,619
Number of sequences in database: 2,121,173
Lambda K H
0.320 0.136 0.391
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 594415034
Number of Sequences: 2121173
Number of extensions: 7095461
Number of successful extensions: 32262
Number of sequences better than 10.0: 65
Number of HSP's better than 10.0 without gapping: 34
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 32210
Number of HSP's gapped (non-prelim): 40
length of query: 725
length of database: 266,710,539
effective HSP length: 59
effective length of query: 666
effective length of database: 141,561,332
effective search space: 94279847112
effective search space used: 94279847112
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.8 bits)
S2: 74 (33.2 bits)
