Score E
Sequences producing significant alignments: (bits) Value
gb|AW249052.1|AW249052 2821050.5prime NIH_MGC_7 Homo sapien... 185 2e-46
gb|AW239340.1|AW239340 xb39c12.y1 NCI_CGAP_Lu31 Homo sapien... 185 2e-46
gb|AW328389.1|AW328389 ds02c04.x1 NIH_MGC_4 Homo sapiens cD... 185 2e-46
gb|AI133547.1|AI133547 HA2162 Human fetal liver cDNA librar... 185 2e-46
gb|AW672800.1|AW672800 ba01g02.y1 NIH_MGC_7 Homo sapiens cD... 183 7e-46
gb|AA313237.1|AA313237 EST185140 Colon carcinoma (HCC) cell... 183 9e-46
gb|BE018174.1|BE018174 bb76c07.y1 NIH_MGC_12 Homo sapiens c... 177 5e-44
gb|AA398573.1|AA398573 zt73h01.s1 Soares_testis_NHT Homo sa... 173 8e-43
dbj|D56048.1|D56048 HUM413E11B Clontech human fetal brain p... 137 2e-42
gb|BE018517.1|BE018517 bb81e04.y1 NIH_MGC_10 Homo sapiens c... 172 2e-42
gb|AI024045.1|AI024045 ov72a12.s1 Soares_testis_NHT Homo sa... 169 1e-41
gb|AA393505.1|AA393505 zt73h01.r1 Soares_testis_NHT Homo sa... 167 8e-41
gb|AA311427.1|AA311427 EST182225 Jurkat T-cells V Homo sapi... 166 1e-40
gb|AW407006.1|AW407006 UI-HF-BL0-adf-d-10-0-UI.r1 NIH_MGC_3... 164 4e-40
gb|AW239009.1|AW239009 xb35g12.y1 NCI_CGAP_Lu31 Homo sapien... 161 3e-39
gb|AA310932.1|AA310932 EST181910 Jurkat T-cells V Homo sapi... 142 1e-36
gb|AW410315.1|AW410315 fh05a05.x1 NIH_MGC_17 Homo sapiens c... 144 7e-34
gb|W76663.1|W76663 zd63g08.r1 Soares_fetal_heart_NbHH19W Ho... 139 1e-32
gb|AW238868.1|AW238868 xb29a08.y1 NCI_CGAP_Lu31 Homo sapien... 92 2e-32
gb|AW994205.1|AW994205 RC3-BN0036-250200-012-g02 BN0036 Hom... 138 3e-32
gb|AW406978.1|AW406978 UI-HF-BL0-adf-a-11-0-UI.r1 NIH_MGC_3... 138 5e-32
gb|AA356950.1|AA356950 EST65583 Jurkat T-cells III Homo sap... 133 1e-30
gb|BE088481.1|BE088481 CM2-BT0688-210300-122-f06 BT0688 Hom... 128 4e-29
gb|R97857.1|R97857 yq60d05.r1 Soares fetal liver spleen 1NF... 123 1e-27
gb|AA171878.1|AA171878 zp22e02.r1 Stratagene neuroepitheliu... 107 2e-27
gb|AW374316.1|AW374316 QV0-CT0018-011199-042-g09 CT0018 Hom... 109 2e-27
gb|AW374295.1|AW374295 QV0-CT0018-011199-042-b06 CT0018 Hom... 108 2e-26
gb|AA309852.1|AA309852 EST18092 Aorta endothelial cells, TN... 116 1e-25
gb|AW580444.1|AW580444 PM1-HT0452-060100-002-f12 HT0452 Hom... 79 1e-25
gb|AW374293.1|AW374293 QV0-CT0018-011199-042-b01 CT0018 Hom... 109 3e-25
gb|N28521.1|N28521 yx37a07.r1 Soares melanocyte 2NbHM Homo ... 115 4e-25
gb|AA315126.1|AA315126 EST187066 Colon carcinoma (HCC) cell... 110 8e-24
gb|BE073293.1|BE073293 MR0-BT0551-060300-103-b06 BT0551 Hom... 110 1e-23
gb|AW805245.1|AW805245 QV1-UM0099-110400-144-f07 UM0099 Hom... 108 5e-23
gb|AW238971.1|AW238971 xb35b12.y1 NCI_CGAP_Lu31 Homo sapien... 105 3e-22
gb|AW246420.1|AW246420 2822249.5prime NIH_MGC_7 Homo sapien... 101 4e-21
gb|AA171556.1|AA171556 zp23e12.r1 Stratagene neuroepitheliu... 99 2e-20
gb|AW805249.1|AW805249 QV1-UM0099-110400-144-g05 UM0099 Hom... 99 3e-20
gb|AW580439.1|AW580439 PM1-HT0452-060100-002-c11 HT0452 Hom... 95 4e-19
gb|AA292205.1|AA292205 zt50g01.r1 Soares ovary tumor NbHOT ... 92 3e-18
gb|AI650523.1|AI650523 wa92a04.x1 NCI_CGAP_GC6 Homo sapiens... 89 3e-17
gb|BE168340.1|BE168340 QV3-HT0514-150300-119-g10 HT0514 Hom... 89 3e-17
gb|BE088484.1|BE088484 CM2-BT0688-210300-122-h04 BT0688 Hom... 89 4e-17
gb|AA353842.1|AA353842 EST62293 Jurkat T-cells V Homo sapie... 88 7e-17
gb|AW376364.1|AW376364 RC5-CT0210-240999-011-b01 CT0210 Hom... 76 1e-16
gb|U46419.1|U46419 HSU46419 Human pancreatic cancer cell li... 85 3e-16
gb|AA306616.1|AA306616 EST177561 Jurkat T-cells VI Homo sap... 73 3e-13
gb|AW938626.1|AW938626 PM1-DT0063-160200-003-f05 DT0063 Hom... 73 2e-12
gb|AI940632.1|AI940632 IL3-ST0024-230799-001-C09 ST0024 Hom... 73 2e-12
gb|T29619.1|T29619 EST87598 Human Lung Homo sapiens cDNA 5'... 70 1e-11
Alignments
>gb|AW249052.1|AW249052 2821050.5prime NIH_MGC_7 Homo sapiens cDNA clone IMAGE:2821050 5'.
Length = 582
Score = 185 bits (465), Expect = 2e-46
Identities = 89/154 (57%), Positives = 119/154 (76%)
Frame = +1
Query: 7 DEEQFDSAESGAAATFPKQCSALRKNEHVMIRGRPCKIVEMSTSKTGKHGHAKVHMVAID 66
D+ F++ ++GA+ATFP QCSALRKN V+++GRPCKIVEMSTSKTGKHGHAKVH+V ID
Sbjct: 10 DDLDFETGDAGASATFPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 189
Query: 67 IFTTKKLEDICPSTHNMDVPVVKRREYILMSIEDGFCSLMDPESCELKDDLKMPEGDLGN 126
IFT KK EDICPSTHNMDVP +KR ++ L+ I+DG+ SL+ +S E+++DL++PEGDLG
Sbjct: 190 IFTGKKYEDICPSTHNMDVPNIKRNDFQLIGIQDGYLSLLQ-DSGEVREDLRLPEGDLGK 366
Query: 127 TIREALEKDEGSVLVQVVAACGEEAILGYKISTK 160
I + + E +L+ V++A EEA + K K
Sbjct: 367 EIEQKYDCGE-EILITVLSAMTEEAAVAIKAMAK 465
>gb|AW239340.1|AW239340 xb39c12.y1 NCI_CGAP_Lu31 Homo sapiens cDNA clone IMAGE:2578678 5'
similar to gb:M23419 INITIATION FACTOR 5A (HUMAN);.
Length = 610
Score = 185 bits (465), Expect = 2e-46
Identities = 89/154 (57%), Positives = 119/154 (76%)
Frame = +2
Query: 7 DEEQFDSAESGAAATFPKQCSALRKNEHVMIRGRPCKIVEMSTSKTGKHGHAKVHMVAID 66
D+ F++ ++GA+ATFP QCSALRKN V+++GRPCKIVEMSTSKTGKHGHAKVH+V ID
Sbjct: 101 DDLDFETGDAGASATFPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 280
Query: 67 IFTTKKLEDICPSTHNMDVPVVKRREYILMSIEDGFCSLMDPESCELKDDLKMPEGDLGN 126
IFT KK EDICPSTHNMDVP +KR ++ L+ I+DG+ SL+ +S E+++DL++PEGDLG
Sbjct: 281 IFTGKKYEDICPSTHNMDVPNIKRNDFQLIGIQDGYLSLLQ-DSGEVREDLRLPEGDLGK 457
Query: 127 TIREALEKDEGSVLVQVVAACGEEAILGYKISTK 160
I + + E +L+ V++A EEA + K K
Sbjct: 458 EIEQKYDCGE-EILITVLSAMTEEAAVAIKAMAK 556
>gb|AW328389.1|AW328389 ds02c04.x1 NIH_MGC_4 Homo sapiens cDNA clone IMAGE:2847343 5'.
Length = 528
Score = 185 bits (465), Expect = 2e-46
Identities = 89/154 (57%), Positives = 119/154 (76%)
Frame = +2
Query: 7 DEEQFDSAESGAAATFPKQCSALRKNEHVMIRGRPCKIVEMSTSKTGKHGHAKVHMVAID 66
D+ F++ ++GA+ATFP QCSALRKN V+++GRPCKIVEMSTSKTGKHGHAKVH+V ID
Sbjct: 8 DDLDFETGDAGASATFPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 187
Query: 67 IFTTKKLEDICPSTHNMDVPVVKRREYILMSIEDGFCSLMDPESCELKDDLKMPEGDLGN 126
IFT KK EDICPSTHNMDVP +KR ++ L+ I+DG+ SL+ +S E+++DL++PEGDLG
Sbjct: 188 IFTGKKYEDICPSTHNMDVPNIKRNDFQLIGIQDGYLSLLQ-DSGEVREDLRLPEGDLGK 364
Query: 127 TIREALEKDEGSVLVQVVAACGEEAILGYKISTK 160
I + + E +L+ V++A EEA + K K
Sbjct: 365 EIEQKYDCGE-EILITVLSAMTEEAAVAIKAMAK 463
>gb|AI133547.1|AI133547 HA2162 Human fetal liver cDNA library Homo sapiens cDNA.
Length = 644
Score = 185 bits (465), Expect = 2e-46
Identities = 89/154 (57%), Positives = 119/154 (76%)
Frame = +2
Query: 7 DEEQFDSAESGAAATFPKQCSALRKNEHVMIRGRPCKIVEMSTSKTGKHGHAKVHMVAID 66
D+ F++ ++GA+ATFP QCSALRKN V+++GRPCKIVEMSTSKTGKHGHAKVH+V ID
Sbjct: 68 DDLDFETGDAGASATFPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 247
Query: 67 IFTTKKLEDICPSTHNMDVPVVKRREYILMSIEDGFCSLMDPESCELKDDLKMPEGDLGN 126
IFT KK EDICPSTHNMDVP +KR ++ L+ I+DG+ SL+ +S E+++DL++PEGDLG
Sbjct: 248 IFTGKKYEDICPSTHNMDVPNIKRNDFQLIGIQDGYLSLLQ-DSGEVREDLRLPEGDLGK 424
Query: 127 TIREALEKDEGSVLVQVVAACGEEAILGYKISTK 160
I + + E +L+ V++A EEA + K K
Sbjct: 425 EIEQKYDCGE-EILITVLSAMTEEAAVAIKAMAK 523
>gb|AW672800.1|AW672800 ba01g02.y1 NIH_MGC_7 Homo sapiens cDNA clone IMAGE:2823122 5'
similar to gb:M23419 INITIATION FACTOR 5A (HUMAN);.
Length = 508
Score = 183 bits (461), Expect = 7e-46
Identities = 89/150 (59%), Positives = 116/150 (77%)
Frame = +3
Query: 11 FDSAESGAAATFPKQCSALRKNEHVMIRGRPCKIVEMSTSKTGKHGHAKVHMVAIDIFTT 70
FD ++GA+ATFP QCSALRKN V+++GRPCKIVEMSTSKTGKHGHAKVH+V IDIFT
Sbjct: 12 FDIGDAGASATFPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGIDIFTG 191
Query: 71 KKLEDICPSTHNMDVPVVKRREYILMSIEDGFCSLMDPESCELKDDLKMPEGDLGNTIRE 130
KK EDICPSTHNMDVP +KR ++ L+ I+DG+ SL+ +S E+++DL++PEGDLG I +
Sbjct: 192 KKYEDICPSTHNMDVPNIKRNDFQLIGIQDGYLSLLQ-DSGEVREDLRLPEGDLGKEIEQ 368
Query: 131 ALEKDEGSVLVQVVAACGEEAILGYKISTK 160
+ E +L+ V++A EEA + K K
Sbjct: 369 KYDCGE-EILITVLSAMTEEAAVAIKAMAK 455
>gb|AA313237.1|AA313237 EST185140 Colon carcinoma (HCC) cell line Homo sapiens cDNA 5' end
similar to translation initiation factor 4D.
Length = 497
Score = 183 bits (460), Expect = 9e-46
Identities = 88/150 (58%), Positives = 117/150 (77%)
Frame = +3
Query: 11 FDSAESGAAATFPKQCSALRKNEHVMIRGRPCKIVEMSTSKTGKHGHAKVHMVAIDIFTT 70
F++ ++GA+ATFP QCSALRKN V+++GRPCKIVEMSTSKTGKHGHAKVH+V IDIFT
Sbjct: 6 FETGDAGASATFPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGIDIFTG 185
Query: 71 KKLEDICPSTHNMDVPVVKRREYILMSIEDGFCSLMDPESCELKDDLKMPEGDLGNTIRE 130
KK EDICPSTHNMDVP +KR ++ L+ I+DG+ SL+ +S E+++DL++PEGDLG I +
Sbjct: 186 KKYEDICPSTHNMDVPNIKRNDFQLIGIQDGYLSLLQ-DSGEVREDLRLPEGDLGKEIEQ 362
Query: 131 ALEKDEGSVLVQVVAACGEEAILGYKISTK 160
+ E +L+ V++A EEA + K K
Sbjct: 363 KYDCGE-EILITVLSAMTEEAAVAIKAMAK 449
>gb|BE018174.1|BE018174 bb76c07.y1 NIH_MGC_12 Homo sapiens cDNA clone IMAGE:3048300 5'
similar to gb:M23419 INITIATION FACTOR 5A (HUMAN);.
Length = 530
Score = 177 bits (445), Expect = 5e-44
Identities = 84/140 (60%), Positives = 112/140 (80%)
Frame = +2
Query: 7 DEEQFDSAESGAAATFPKQCSALRKNEHVMIRGRPCKIVEMSTSKTGKHGHAKVHMVAID 66
D+ F++ ++GA+ATFP QCSALRKN V+++GRPCKIVEMSTSKTGKHGHAKVH+V ID
Sbjct: 110 DDLDFETGDAGASATFPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 289
Query: 67 IFTTKKLEDICPSTHNMDVPVVKRREYILMSIEDGFCSLMDPESCELKDDLKMPEGDLGN 126
IFT KK EDICPSTHNMDVP +KR ++ L+ I DG+ SL+ +S E+++DL++PEGDLG
Sbjct: 290 IFTGKKYEDICPSTHNMDVPNIKRNDFQLIGIHDGYLSLLQ-DSGEVREDLRLPEGDLGR 466
Query: 127 TIREALEKDEGSVLVQVVAA 146
I + + E +L+ V++A
Sbjct: 467 EIEQKYDCGE-EILITVLSA 523
>gb|AA398573.1|AA398573 zt73h01.s1 Soares_testis_NHT Homo sapiens cDNA clone IMAGE:728017
3' similar to gb:M23419 INITIATION FACTOR 5A (HUMAN);.
Length = 473
Score = 173 bits (435), Expect = 8e-43
Identities = 85/141 (60%), Positives = 107/141 (75%)
Frame = +2
Query: 7 DEEQFDSAESGAAATFPKQCSALRKNEHVMIRGRPCKIVEMSTSKTGKHGHAKVHMVAID 66
DE F + ++GA++T+P QCSALRKN V+++GRPCKIVEMSTSKTGKHGHAKVH+V ID
Sbjct: 53 DEIDFTTGDAGASSTYPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 232
Query: 67 IFTTKKLEDICPSTHNMDVPVVKRREYILMSIEDGFCSLMDPESCELKDDLKMPEGDLGN 126
IFT KK EDICPSTHNMDVP +KR +Y L+ I+DG+ SL+ E+ E+++DLK+PEG+LG
Sbjct: 233 IFTGKKYEDICPSTHNMDVPNIKRNDYQLICIQDGYLSLL-TETGEVREDLKLPEGELGK 409
Query: 127 TIREALEKDEGSVLVQVVAAC 147
I E VQV C
Sbjct: 410 EIEGKYNAGED---VQVSVMC 463
>dbj|D56048.1|D56048 HUM413E11B Clontech human fetal brain polyA+ mRNA (#6535) Homo
sapiens cDNA clone GEN-413E11 5'.
Length = 601
Score = 137 bits (342), Expect(2) = 2e-42
Identities = 62/86 (72%), Positives = 73/86 (84%)
Frame = +2
Query: 4 DHHDEEQFDSAESGAAATFPKQCSALRKNEHVMIRGRPCKIVEMSTSKTGKHGHAKVHMV 63
+ D+ F++ ++GA+ATFP QCSALRKN V+++GRPCKIVEMSTSKTGKHGHAKVH+V
Sbjct: 104 NRQDDLDFETGDAGASATFPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLV 283
Query: 64 AIDIFTTKKLEDICPSTHNMDVPVVK 89
IDIFT KK EDICPSTHNMDVP K
Sbjct: 284 GIDIFTGKKYEDICPSTHNMDVPTSK 361
Score = 56.6 bits (134), Expect(2) = 2e-42
Identities = 29/75 (38%), Positives = 49/75 (64%)
Frame = +1
Query: 86 PVVKRREYILMSIEDGFCSLMDPESCELKDDLKMPEGDLGNTIREALEKDEGSVLVQVVA 145
P +KR ++ L+ I+DG+ SL+ +S E+++DL++PEGDLG I + + E +L+ V++
Sbjct: 349 PNIKRNDFQLIGIQDGYLSLLQ-DSGEVREDLRLPEGDLGKEIEQKYDCGE-EILITVLS 522
Query: 146 ACGEEAILGYKISTK 160
A EEA + K K
Sbjct: 523 AMTEEAAVAIKAMAK 567
>gb|BE018517.1|BE018517 bb81e04.y1 NIH_MGC_10 Homo sapiens cDNA clone IMAGE:3048798 5'
similar to gb:M23419 INITIATION FACTOR 5A (HUMAN);.
Length = 586
Score = 172 bits (432), Expect = 2e-42
Identities = 80/132 (60%), Positives = 106/132 (79%)
Frame = +1
Query: 7 DEEQFDSAESGAAATFPKQCSALRKNEHVMIRGRPCKIVEMSTSKTGKHGHAKVHMVAID 66
D+ F++ ++GA+ATFP QCSALRKN V+++GRPCKIVEMSTSKTGKHGHAKVH+V ID
Sbjct: 181 DDLDFETGDAGASATFPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 360
Query: 67 IFTTKKLEDICPSTHNMDVPVVKRREYILMSIEDGFCSLMDPESCELKDDLKMPEGDLGN 126
IFT KK EDICPSTHNMDVP +KR ++ L+ I+DG+ SL+ +S E+++DL +PEGDLG
Sbjct: 361 IFTGKKYEDICPSTHNMDVPNIKRNDFQLIGIQDGYLSLLQ-DSGEVREDLLLPEGDLGK 537
Query: 127 TIREALEKDEGS 138
+ + ++ S
Sbjct: 538 VMSRSTTVEKRS 573
Database: GenBank Human EST entries
Posted date: Jul 9, 2000 8:11 PM
Number of letters in database: 800,131,619
Number of sequences in database: 2,121,173
Lambda K H
0.315 0.132 0.379
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 148938429
Number of Sequences: 2121173
Number of extensions: 1702186
Number of successful extensions: 5486
Number of sequences better than 10.0: 114
Number of HSP's better than 10.0 without gapping: 77
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 5380
Number of HSP's gapped (non-prelim): 82
length of query: 161
length of database: 266,710,539
effective HSP length: 56
effective length of query: 105
effective length of database: 147,924,851
effective search space: 15532109355
effective search space used: 15532109355
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.6 bits)
S2: 68 (30.9 bits)
