Score E
Sequences producing significant alignments: (bits) Value
pir||S41010 translation initiation factor eIF-5A - Caenorha... 332 2e-90
sp|P34563|IF51_CAEEL PROBABLE INITIATION FACTOR 5A (EIF-5A)... 332 2e-90
sp|Q20751|IF52_CAEEL PROBABLE INITIATION FACTOR 5A (EIF-5A)... 289 1e-77
sp|O94083|IF5A_CANAL INITIATION FACTOR 5A (EIF-5A) (EIF-4D)... 191 3e-48
ref|NP_010880.1| Translation initiation factor eIF-5A; Hyp2... 190 4e-48
ref|NP_012581.1| anaerobically expressed form of translatio... 187 5e-47
sp|P10160|IF5A_RABIT INITIATION FACTOR 5A (EIF-5A) (EIF-4D)... 186 9e-47
pir||T40248 initiation factor eif-5a - fission yeast (Schiz... 186 1e-46
sp|P56289|IF5A_SCHPO INITIATION FACTOR EIF-5A >gi|7492107|p... 185 1e-46
ref|NP_001961.1| eukaryotic translation initiation factor 5... 185 2e-46
gb|AAF47151.1| (AE003462) CG3186 gene product [Drosophila m... 184 4e-46
gb|AAF13315.1|AF109730_1 (AF109730) translation initiation ... 183 6e-46
gb|AAF80375.1|AF159443_1 (AF159443) eukaryotic initiation f... 182 2e-45
sp|Q07460|IF51_CHICK INITIATION FACTOR 5A-1 (EIF-5A) (EIF-4... 181 3e-45
pir||I53801 gene eif-5A protein - human >gi|4261795|gb|AAD1... 178 3e-44
sp|P38672|IF5A_NEUCR INITIATION FACTOR 5A (EIF-5A) (EIF-4D)... 176 7e-44
sp|P13651|IF5A_DICDI INITIATION FACTOR 5A (EIF-5A) (EIF-4D)... 176 1e-43
sp|P24922|IF52_NICPL INITIATION FACTOR 5A-2 (EIF-5A) (EIF-4... 155 3e-37
gb|AAF67762.1| (AC018364) putative eukaryotic initiation fa... 154 5e-37
sp|P24921|IF51_NICPL INITIATION FACTOR 5A-1 (EIF-5A) (EIF-4... 154 5e-37
gb|AAD39281.1|AC007576_4 (AC007576) initiation factor 5A-4 ... 152 1e-36
sp|P56333|IF51_SOLTU INITIATION FACTOR 5A (EIF-5A) (EIF-4D)... 152 2e-36
sp|P56337|IF55_SOLTU INITIATION FACTOR 5A-5 (EIF-5A) (EIF-4... 151 3e-36
sp|P56335|IF53_SOLTU INITIATION FACTOR 5A-3 (EIF-5A) (EIF-4... 151 4e-36
gb|AAC67555.1| (AF094773) translation initiation factor 5A ... 150 7e-36
sp|P56336|IF54_SOLTU INITIATION FACTOR 5A-4 (EIF-5A) (EIF-4... 149 2e-35
emb|CAB96075.1| (AJ252135) translation initiation factor, e... 149 2e-35
sp|P56332|IF5A_MAIZE INITIATION FACTOR 5A (EIF-5A) (EIF-4D)... 148 4e-35
pir||S21058 translation initiation factor eIF-5A.1 - curled... 146 8e-35
sp|P26564|IF5A_MEDSA INITIATION FACTOR 5A (EIF-5A) (EIF-4D)... 146 1e-34
emb|CAB65463.1| (AJ238624) translation initiation factor 5A... 146 1e-34
sp|Q09121|IF52_CHICK INITIATION FACTOR 5A-2 (EIF-5A) (EIF-4D) 145 2e-34
gb|AAF27938.1|AF225297_1 (AF225297) translation initiation ... 141 3e-33
gb|AAF79401.1|AC068197_11 (AC068197) F16A14.17 [Arabidopsis... 135 2e-31
pir||B42156 translation initiation factor eIF-5A II - chick... 128 3e-29
emb|CAB95733.1| (AJ278539) eukaryotic initiation factor 5a ... 121 4e-27
gb|AAB21928.1| eukaryotic translation initiation factor 5A ... 115 3e-25
gb|AAB21933.1| eukaryotic translation initiation factor 5A ... 109 2e-23
sp|Q26571|IF5A_SCHMA INITIATION FACTOR 5A-2 (EIF-5A) >gi|73... 80 1e-14
sp|P28461|IF5A_SULAC INITIATION FACTOR 5A (IF-5A) (HYPUSINE... 75 3e-13
pir||C64453 translation initiation factor eIF-5a homolog - ... 73 1e-12
sp|Q58625|IF5A_METJA INITIATION FACTOR 5A (IF-5A) (HYPUSINE... 73 1e-12
pdb|2EIF|A Chain A, Eukaryotic Translation Initiation Facto... 73 1e-12
pdb|1EIF| Eukaryotic Translation Initiation Factor 5a Fr... 73 1e-12
sp|Q9YA53|IF5A_AERPE INITIATION FACTOR 5A (IF-5A) (HYPUSINE... 70 1e-11
sp|O29612|IF5A_ARCFU INITIATION FACTOR 5A (IF-5A) (HYPUSINE... 66 2e-10
sp|P56635|IF5A_PYRAE INITIATION FACTOR 5A (IF-5A) (HYPUSINE... 62 3e-09
pir||H75120 probable initiation factor 5a (aif5a) PAB1854 -... 61 9e-09
sp|O26955|IF5A_METTH INITIATION FACTOR 5A (IF-5A) (HYPUSINE... 60 1e-08
sp|O50089|IF5A_PYRHO INITIATION FACTOR 5A (IF-5A) (HYPUSINE... 60 1e-08
Alignments
>pir||S41010 translation initiation factor eIF-5A - Caenorhabditis elegans
Length = 173
Score = 332 bits (841), Expect = 2e-90
Identities = 161/161 (100%), Positives = 161/161 (100%)
Query: 1 MSEDHHDEEQFDSAESGAAATFPKQCSALRKNEHVMIRGRPCKIVEMSTSKTGKHGHAKV 60
MSEDHHDEEQFDSAESGAAATFPKQCSALRKNEHVMIRGRPCKIVEMSTSKTGKHGHAKV
Sbjct: 13 MSEDHHDEEQFDSAESGAAATFPKQCSALRKNEHVMIRGRPCKIVEMSTSKTGKHGHAKV 72
Query: 61 HMVAIDIFTTKKLEDICPSTHNMDVPVVKRREYILMSIEDGFCSLMDPESCELKDDLKMP 120
HMVAIDIFTTKKLEDICPSTHNMDVPVVKRREYILMSIEDGFCSLMDPESCELKDDLKMP
Sbjct: 73 HMVAIDIFTTKKLEDICPSTHNMDVPVVKRREYILMSIEDGFCSLMDPESCELKDDLKMP 132
Query: 121 EGDLGNTIREALEKDEGSVLVQVVAACGEEAILGYKISTKE 161
EGDLGNTIREALEKDEGSVLVQVVAACGEEAILGYKISTKE
Sbjct: 133 EGDLGNTIREALEKDEGSVLVQVVAACGEEAILGYKISTKE 173
>sp|P34563|IF51_CAEEL PROBABLE INITIATION FACTOR 5A (EIF-5A) (EIF-4D)
emb|CAA81597.1| (Z27079) similar to initiation factor 5A~cDNA EST EMBL:D74625 comes
from this gene~cDNA EST EMBL:D75463 comes from this
gene~cDNA EST EMBL:D75831 comes from this gene~cDNA EST
EMBL:D72879 comes from this gene~cDNA EST EMBL:D76399
comes from this gene~c>
Length = 161
Score = 332 bits (841), Expect = 2e-90
Identities = 161/161 (100%), Positives = 161/161 (100%)
Query: 1 MSEDHHDEEQFDSAESGAAATFPKQCSALRKNEHVMIRGRPCKIVEMSTSKTGKHGHAKV 60
MSEDHHDEEQFDSAESGAAATFPKQCSALRKNEHVMIRGRPCKIVEMSTSKTGKHGHAKV
Sbjct: 1 MSEDHHDEEQFDSAESGAAATFPKQCSALRKNEHVMIRGRPCKIVEMSTSKTGKHGHAKV 60
Query: 61 HMVAIDIFTTKKLEDICPSTHNMDVPVVKRREYILMSIEDGFCSLMDPESCELKDDLKMP 120
HMVAIDIFTTKKLEDICPSTHNMDVPVVKRREYILMSIEDGFCSLMDPESCELKDDLKMP
Sbjct: 61 HMVAIDIFTTKKLEDICPSTHNMDVPVVKRREYILMSIEDGFCSLMDPESCELKDDLKMP 120
Query: 121 EGDLGNTIREALEKDEGSVLVQVVAACGEEAILGYKISTKE 161
EGDLGNTIREALEKDEGSVLVQVVAACGEEAILGYKISTKE
Sbjct: 121 EGDLGNTIREALEKDEGSVLVQVVAACGEEAILGYKISTKE 161
>sp|Q20751|IF52_CAEEL PROBABLE INITIATION FACTOR 5A (EIF-5A) (EIF-4D)
pir||T22628 hypothetical protein F54C9.1 - Caenorhabditis elegans
emb|CAA90247.1| (Z49967) similar to initiation factor 5A~cDNA EST EMBL:D75952 comes
from this gene~cDNA EST EMBL:D74070 comes from this
gene~cDNA EST EMBL:D74623 comes from this gene~cDNA EST
EMBL:D74571 comes from this gene~cDNA EST EMBL:D76331
comes from this gene~c>
Length = 161
Score = 289 bits (732), Expect = 1e-77
Identities = 135/161 (83%), Positives = 152/161 (93%)
Query: 1 MSEDHHDEEQFDSAESGAAATFPKQCSALRKNEHVMIRGRPCKIVEMSTSKTGKHGHAKV 60
MS+DHHD+E F + +SGAAATFPKQCSALRKNEHVMI+GRPCKIVEMSTSKTGKHGHAKV
Sbjct: 1 MSDDHHDDEHFHTGDSGAAATFPKQCSALRKNEHVMIKGRPCKIVEMSTSKTGKHGHAKV 60
Query: 61 HMVAIDIFTTKKLEDICPSTHNMDVPVVKRREYILMSIEDGFCSLMDPESCELKDDLKMP 120
HMVAIDIFT+KKLEDICPSTHNMDVPVVKRREY+LM+I+DG+CSLMDPESCE KDDLK+P
Sbjct: 61 HMVAIDIFTSKKLEDICPSTHNMDVPVVKRREYLLMAIDDGYCSLMDPESCEQKDDLKLP 120
Query: 121 EGDLGNTIREALEKDEGSVLVQVVAACGEEAILGYKISTKE 161
+ +LG IR+A EKDEGSVLVQVV+A GEEAILG+K+STKE
Sbjct: 121 DTELGQQIRDAYEKDEGSVLVQVVSAIGEEAILGWKVSTKE 161
>sp|O94083|IF5A_CANAL INITIATION FACTOR 5A (EIF-5A) (EIF-4D)
gb|AAD10697.1| (U07366) eIF-5A [Candida albicans]
Length = 157
Score = 191 bits (480), Expect = 3e-48
Identities = 93/155 (60%), Positives = 125/155 (80%), Gaps = 4/155 (2%)
Query: 7 DEEQFDSAESGAAATFPKQCSALRKNEHVMIRGRPCKIVEMSTSKTGKHGHAKVHMVAID 66
+E+ F++A++GAA TFP QCSALRKN HV+I+ RPCKIV+MSTSKTGKHGHAKVH+VAID
Sbjct: 3 EEDTFETADAGAALTFPMQCSALRKNGHVVIKNRPCKIVDMSTSKTGKHGHAKVHLVAID 62
Query: 67 IFTTKKLEDICPSTHNMDVPVVKRREYILMSIEDGFCSLMDPESCELKDDLKMPEGDLGN 126
IFT KKLED+ PSTHNM+VP V R+E+ L+ I+DG+ SLM + + KDD+K+PEG+LG+
Sbjct: 63 IFTGKKLEDLSPSTHNMEVPNVSRQEFQLLDIDDGYLSLMTADG-DTKDDVKVPEGELGD 121
Query: 127 TIREALEKDEG-SVLVQVVAACGEEAILGYKISTK 160
++ E DEG ++V +++A GEEA + YK + K
Sbjct: 122 KLQS--EFDEGKDLIVTIISAMGEEAAISYKEAPK 154
>ref|NP_010880.1| Translation initiation factor eIF-5A; Hyp2p
sp|P23301|IF52_YEAST INITIATION FACTOR 5A-2 (EIF-5A) (EIF-4D) (HYPUSINE CONTAINING
PROTEIN HP2)
pir||FIBYA1 translation initiation factor eIF-5A.1 - yeast (Saccharomyces
cerevisiae)
emb|CAA39693.1| (X56236) hypusine containing protein HP2 [Saccharomyces
cerevisiae]
gb|AAA35155.1| (M63541) initiation factor 5A [Saccharomyces cerevisiae]
gb|AAB65008.1| (U18779) Hyp2p: translation initiation factor eIF-5A [Saccharomyces
cerevisiae]
dbj|BAA11826.1| (D83166) eukaryotic translation initiation factor 5A precursor
[Saccharomyces cerevisiae]
Length = 157
Score = 190 bits (479), Expect = 4e-48
Identities = 92/151 (60%), Positives = 124/151 (81%), Gaps = 4/151 (2%)
Query: 7 DEEQFDSAESGAAATFPKQCSALRKNEHVMIRGRPCKIVEMSTSKTGKHGHAKVHMVAID 66
+E F++A++G++AT+P QCSALRKN V+I+ RPCKIV+MSTSKTGKHGHAKVH+VAID
Sbjct: 4 EEHTFETADAGSSATYPMQCSALRKNGFVVIKSRPCKIVDMSTSKTGKHGHAKVHLVAID 63
Query: 67 IFTTKKLEDICPSTHNMDVPVVKRREYILMSIEDGFCSLMDPESCELKDDLKMPEGDLGN 126
IFT KKLED+ PSTHNM+VPVVKR EY L+ I+DGF SLM+ + + KDD+K PEG+LG+
Sbjct: 64 IFTGKKLEDLSPSTHNMEVPVVKRNEYQLLDIDDGFLSLMNMDG-DTKDDVKAPEGELGD 122
Query: 127 TIREALEKDEG-SVLVQVVAACGEEAILGYK 156
+++ A DEG ++V +++A GEEA + +K
Sbjct: 123 SLQTAF--DEGKDLMVTIISAMGEEAAISFK 151
>ref|NP_012581.1| anaerobically expressed form of translation initiation factor
eIF-5A; Anb1p
sp|P19211|IF51_YEAST INITIATION FACTOR 5A-1 (EIF-5A) (EIF-4D) (HYPUSINE CONTAINING
PROTEIN HP1)
pir||FIBYA2 translation initiation factor eIF-5A.2 - yeast (Saccharomyces
cerevisiae)
emb|CAA39692.1| (X56235) hypusine containing protein HP1 [Saccharomyces
cerevisiae]
gb|AAA34425.1| (J05455) protein synthesis initiation factor (eIF-4D)
[Saccharomyces cerevisiae]
gb|AAA35156.1| (M63542) initiation factor 5A [Saccharomyces cerevisiae]
emb|CAA89575.1| (Z49547) ORF YJR047c [Saccharomyces cerevisiae]
gb|AAA88750.1| (L36344) ORF; putative [Saccharomyces cerevisiae]
Length = 157
Score = 187 bits (470), Expect = 5e-47
Identities = 90/151 (59%), Positives = 122/151 (80%), Gaps = 4/151 (2%)
Query: 7 DEEQFDSAESGAAATFPKQCSALRKNEHVMIRGRPCKIVEMSTSKTGKHGHAKVHMVAID 66
+E F++A++GA+AT+P QCSALRKN V+I+GRPCKIV+MSTSKTGKHGHAKVH+V +D
Sbjct: 4 EEHTFENADAGASATYPMQCSALRKNGFVVIKGRPCKIVDMSTSKTGKHGHAKVHLVTLD 63
Query: 67 IFTTKKLEDICPSTHNMDVPVVKRREYILMSIEDGFCSLMDPESCELKDDLKMPEGDLGN 126
IFT KKLED+ PSTHN++VP VKR EY L+ I+DG+ SLM + E KDD+K PEG+LG+
Sbjct: 64 IFTGKKLEDLSPSTHNLEVPFVKRSEYQLLDIDDGYLSLMTMDG-ETKDDVKAPEGELGD 122
Query: 127 TIREALEKDEG-SVLVQVVAACGEEAILGYK 156
+++ A DEG ++V +++A GEEA + +K
Sbjct: 123 SMQAAF--DEGKDLMVTIISAMGEEAAISFK 151
>sp|P10160|IF5A_RABIT INITIATION FACTOR 5A (EIF-5A) (EIF-4D)
pir||A31486 translation initiation factor eIF-5A - rabbit
Length = 154
Score = 186 bits (468), Expect = 9e-47
Identities = 89/154 (57%), Positives = 119/154 (76%), Gaps = 2/154 (1%)
Query: 7 DEEQFDSAESGAAATFPKQCSALRKNEHVMIRGRPCKIVEMSTSKTGKHGHAKVHMVAID 66
D+ F++ ++GA+ATFP QCSALRKN V+++GRPCKIVEMSTSKTGKHGHAKVH+V ID
Sbjct: 3 DDLDFETGDAGASATFPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 62
Query: 67 IFTTKKLEDICPSTHNMDVPVVKRREYILMSIEDGFCSLMDPESCELKDDLKMPEGDLGN 126
IFT KK EDICPSTHNMDVP +KR ++ L+ I+DG+ SL+ +S E+++DL++PEGDLG
Sbjct: 63 IFTGKKYEDICPSTHNMDVPNIKRNDFQLIGIQDGYLSLLQ-DSGEVREDLRLPEGDLGK 121
Query: 127 TIREALEKDEGSVLVQVVAACGEEAILGYKISTK 160
I + + E +L+ V++A EEA + K K
Sbjct: 122 EIEQKYDSGE-EILITVLSAMTEEAAVAIKAMAK 154
>pir||T40248 initiation factor eif-5a - fission yeast (Schizosaccharomyces
pombe)
emb|CAB58162.1| (AL121815) initiation factor eif-5a [Schizosaccharomyces pombe]
Length = 157
Score = 186 bits (467), Expect = 1e-46
Identities = 91/153 (59%), Positives = 120/153 (77%), Gaps = 4/153 (2%)
Query: 1 MSEDHHDEEQFDSAESGAAATFPKQCSALRKNEHVMIRGRPCKIVEMSTSKTGKHGHAKV 60
M+E+ H + F+ E+GA+ TFP QCSALRKN HV+I+GRPCKIV+MSTSKTGKHGHAKV
Sbjct: 1 MAEEEHVD--FEGGEAGASLTFPMQCSALRKNGHVVIKGRPCKIVDMSTSKTGKHGHAKV 58
Query: 61 HMVAIDIFTTKKLEDICPSTHNMDVPVVKRREYILMSIEDGFCSLMDPESCELKDDLKMP 120
H+VA+DIF +K ED+ PSTHNMDVPVVKR EY L++I+DG+ +LM + KDD+++P
Sbjct: 59 HIVALDIFNGRKYEDMSPSTHNMDVPVVKRDEYQLVNIDDGYLNLMTTDG-TTKDDVRLP 117
Query: 121 EGDLGNTIREALEKDEGSVLVQVVAACGEEAIL 153
EG+LGN I E E+ + +++ VV+A GEE L
Sbjct: 118 EGELGNEIEEGFEEGK-DLIITVVSAMGEEIAL 149
>sp|P56289|IF5A_SCHPO INITIATION FACTOR EIF-5A
pir||T38429 initiation factor eif-5a - fission yeast (Schizosaccharomyces
pombe)
emb|CAB16195.1| (Z99126) initiation factor eif-5a. [Schizosaccharomyces pombe]
Length = 157
Score = 185 bits (466), Expect = 1e-46
Identities = 93/154 (60%), Positives = 120/154 (77%), Gaps = 6/154 (3%)
Query: 1 MSEDHHDEEQFDSAESGAAATFPKQCSALRKNEHVMIRGRPCKIVEMSTSKTGKHGHAKV 60
M+E+ H + F+ E+GA+ TFP QCSALRKN HV+I+GRPCKIV+MSTSKTGKHGHAKV
Sbjct: 1 MAEEEHVD--FEGGEAGASLTFPMQCSALRKNGHVVIKGRPCKIVDMSTSKTGKHGHAKV 58
Query: 61 HMVAIDIFTTKKLEDICPSTHNMDVPVVKRREYILMSIEDGFCSLMDPESCELKDDLKMP 120
H+VA+DIF +K ED+ PSTHNMDVPVVKR EY L++I+DG+ +LM + KDD+++P
Sbjct: 59 HIVALDIFNGRKYEDMSPSTHNMDVPVVKRDEYQLVNIDDGYLNLMTTDG-TTKDDVRLP 117
Query: 121 EGDLGNTIREALEKDEG-SVLVQVVAACGEEAIL 153
EG+LGN I E DEG +++ VV+A GEE L
Sbjct: 118 EGELGNEIEEGF--DEGRDLIITVVSAMGEETAL 149
>ref|NP_001961.1| eukaryotic translation initiation factor 5A
sp|P10159|IF5A_HUMAN INITIATION FACTOR 5A (EIF-5A) (EIF-4D) (REV BINDING FACTOR)
pir||FIHUA translation initiation factor eIF-5A - human
gb|AAA58453.1| (M23419) initiation factor 4D [Homo sapiens]
gb|AAB29229.1| REV binding factor, eukaryotic initiation factor 5A, eIF-5A [human,
HeLa cells, Peptide Partial, 154 aa]
gb|AAA86989.1| (U17969) eIF-5A [Homo sapiens]
Length = 154
Score = 185 bits (465), Expect = 2e-46
Identities = 89/154 (57%), Positives = 119/154 (76%), Gaps = 2/154 (1%)
Query: 7 DEEQFDSAESGAAATFPKQCSALRKNEHVMIRGRPCKIVEMSTSKTGKHGHAKVHMVAID 66
D+ F++ ++GA+ATFP QCSALRKN V+++GRPCKIVEMSTSKTGKHGHAKVH+V ID
Sbjct: 3 DDLDFETGDAGASATFPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 62
Query: 67 IFTTKKLEDICPSTHNMDVPVVKRREYILMSIEDGFCSLMDPESCELKDDLKMPEGDLGN 126
IFT KK EDICPSTHNMDVP +KR ++ L+ I+DG+ SL+ +S E+++DL++PEGDLG
Sbjct: 63 IFTGKKYEDICPSTHNMDVPNIKRNDFQLIGIQDGYLSLLQ-DSGEVREDLRLPEGDLGK 121
Query: 127 TIREALEKDEGSVLVQVVAACGEEAILGYKISTK 160
I + + E +L+ V++A EEA + K K
Sbjct: 122 EIEQKYDCGE-EILITVLSAMTEEAAVAIKAMAK 154
Database: nr
Posted date: Jul 9, 2000 11:06 PM
Number of letters in database: 162,524,432
Number of sequences in database: 517,904
Lambda K H
0.315 0.132 0.379
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 50430001
Number of Sequences: 517904
Number of extensions: 1922416
Number of successful extensions: 4029
Number of sequences better than 10.0: 57
Number of HSP's better than 10.0 without gapping: 52
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 3952
Number of HSP's gapped (non-prelim): 60
length of query: 161
length of database: 162,524,432
effective HSP length: 56
effective length of query: 105
effective length of database: 133,521,808
effective search space: 14019789840
effective search space used: 14019789840
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.6 bits)
S2: 67 (30.5 bits)
