CONTENTS
 Abstract
of our work |
| RNAi
results of the 168 clones
(Bar Graphs : embryonic viability, sterility, and fertilization defect) |
| RNAi
results of the clones that caused some defects
(A Table : Description of the phenotypes) |
| RNAi
results of the clones that caused embryonic or larval lethality |
| RNAi
results of the clones that caused sterility |
Further Information
|
A simple list of the 168 clones |
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A comprehensive list of the 168 clones |
| Search |
| in
situ hybridization results of clones 1 to 21 |
| The germ cells play important roles in transmission of parental genetic information to the next generation and have various characteristics that somatic cells do not have. In order to identify genes involved in germline development, we employed Suppression Subtractive Hybridization (SSH) technique to generate "forward-subtracted" cDNA pool enriched for cDNA derived from meiotic nuclei and "reverse-subtracted" cDNA pool. We then performed differential hybridization using these subtracted cDNA pools as probes on the high-density grid, on which 7,500 EST clones were spotted. One hundred and sixty-eight clones displayed significantly stronger signals with the forward-subtracted cDNA pool. Since it was confirmed by in situ hybridization that subtractive screening described above worked successfully, we subsequently assessed the functions of these genes using the RNAi technique. Fifteen clones showed a range of sterility. These results demonstrate the efficacy of the screening strategy utilizing the EST library and the RNAi technique. |
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| RNAi Phenotypes | ||||||||||||
| Embryonic lethal | Larval lethal | |||||||||||
| Clone
number
1 15 16 20 23 26 112 123 126 134 157 161 164 |
Clone
name
yk37e9 yk499g10 yk538a12 yk602f11 yk41h6 yk118h8 yk507f10 yk391b7 yk486e10 yk540f8 yk585e12 yk595h12 yk605b2 |
CDS
ID
C26E6.4 W06F12.1 F26B1.3 F52E1.1 T22F3.3 M03F8.3 T05G5.7 F20D12.4 T20G5.1 Y45F10A.2 W02A2.h C28C12.2 M28.5 |
Clone
number
14 17 51 123 153 156 164 168 |
Clone
name
yk490e12 yk556g5 yk293a7 yk391b7 yk578a1 yk585c12 yk605b2 yk670b1 |
CDS
ID
K02F2.2 F09F7.3 C47D12.6 F20D12.4 C56A3.8 T01C3.7 M28.5 K07C5.4 |
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| Others | ||||||||||||
| Clone
number
32 50 80 111 128 |
Clone
name
yk199f3 yk286h12 yk383f7 yk506a7 yk529f3 |
CDS
ID
C31C9.2 - F11G11.8 - ZK1151.2 |
Phenotype
Slow growing
Dumpy Pvu Bursting |
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| Sterile (Worms laid unfertilized eggs) | ||||||||||||
| Clone
number
9 10 42 47 121 131 148 |
Clone
name
yk385e11 yk437b3 yk233g4 yk270c1 yk292d2 yk534e2 yk574e1 |
CDS
ID
T03F1.5 C25G4.6 F32A11.3 T25G3.2 R12E2.10 F07A11.2 H02I12.1 |
Homologue
S/T protein
- Chitin synthase Protein tyrosine phosphatase
- |
Phenotype
Only F1 defect, recovers after crossing
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| Sterile (Worms laid unfertilized eggs) | ||||||||||||
| Clone
number
12 50 111 116 127 134 153 160 |
Clone
name
yk445a8 yk286h12 yk506a7 yk519f1 yk520f4 yk540f8 yk578a1 yk590b8 |
CDS
ID
T05G5.10 - - F35G12.10 C05D2.5 Y45F10A.2 C56A3.8 C07H6.5 |
Homologue
eIF-5A - - ATP synthase B chain
Pumilio repeat family
ATP-dependent RNA
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Phenotype
Glp Glp Degenerative nuclei Pachytene arrest Mog Mog, Glp, Gld, Gon Diakinesis defects |
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| clone 1
clone 15 clone 16 clone 20 clone 23 clone 26 clone 112 clone 123 clone 126 clone 134 clone 157 clone 161 clone 164 |
The hatching rate of F1 eggs laid by the P0 worms during a one-day period, starting one day after the injection of dsRNA. The dsRNA for the clones listed on the left was coinjected with dsRNA for GFP. The control group was only injected with dsRNA for GFP. |
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clone 14
clone 17
clone 51
clone 128
clone 153
clone 156
clone 164
clone 168
Sterile
(Worms laid unfertilized eggs)
| clone 9
clone 10 clone 42 clone 47 clone 121 clone 131 clone 148 |
P0 animals were examined for each clone listed on the left. Each animal was categorized according to the number of unfertilized and fertilized eggs it laid during a one-day period, starting one day after the injection of dsRNA. dsRNA for each clone was coinjected with GFP dsRNA. The control group was injected with only GFP dsRNA. |
F1 animals were examined for each clone listed on the left. Each animal was categorized according to the number of unfertilized and fertilized eggs it laid in 10 hours. dsRNA for each clone was coinjected with GFP dsRNA. The control group was injected with only GFP dsRNA. |
| clone 12
clone 50 clone 111 clone 116 clone 127 clone 134 clone 153 clone 160 |
F1 animals were examined for each clone listed on the left. Each animal was categorized according to the number of eggs it laid in 10 hours. dsRNA for each clone was coinjected with GFP dsRNA. The control group was injected with only GFP dsRNA. |
Other phenotypes
clone 32 Slow growing
clone 50 Bursting
clone 80 dumpy
clone 111 Protruded vulva
clone 128 Bursting
Last update was on Nov. 11, 2000