FastQCFastQC Report
Tue 22 Dec 2015
SRR616268sub_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616268sub_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1000000
Sequences flagged as poor quality0
Sequence length107
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCGGTATATTTTCGGCGCAGTGCCACTCGACTAGTGAGCTATTACGCA143831.4383No Hit
GGCCTATTCACTGCGGCTGACCTTGCGGTCAGCACCCCTTCTTCCGAAGT110441.1044No Hit
GTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGG88920.8892000000000001No Hit
CCCGGTATATTTTCGGCGCAGTGCCACTCGACTAGTGAGCTATTACGCAC84740.8474No Hit
GTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACG81890.8189No Hit
GCCGGCATTCTCACTTCTAAGCGCTCCAGCCGTCCTCACGATCGACCTTC81320.8132No Hit
GTCCAGTCCTACAACCCCGAGAAGCAAGCTTCTCGGTTTGGGCTCTTCCC66630.6663No Hit
GTCGGTTTGCGGTACGGGTAGTTTATTTCTCACTAGAAGCTTTTCTTGGC64110.6411No Hit
GGTCACTTGGTTTCGGGTCTACATCTGCTTACTCATTCGCCCTGTTCAGA55020.5502No Hit
GCCGGCATTCTCACTTCTAAGCGCTCCAGCCGTCCTCACGATCAACCTTC48450.48450000000000004No Hit
CCCTCCATCGCTTAAACAAAATAAACTAGTGCAGGAATCTCAACCTGCTT43950.43949999999999995No Hit
CCGGTATATTTTCGGCGCAGTGCCACTCGACTAGTGAGCTATTACGCACT43850.4385No Hit
CCCGCGTCTGCCGCCGGCCAGCTATGTATTCACTGACAAGCAATACACTG43660.4366No Hit
CCACAGTTTCGGTATTATGCTTAGCCCCGGTATATTTTCGGCGCAGTGCC43140.4314No Hit
CTGGGCTGTTCCCCTTTCGACAATGGACCTTATCGCTCACTGTCTGACTC41130.41130000000000005No Hit
CCGCCGTACTCAGGATCCTGGACGGAGGGTTCGACGTTTCGCTTACAGGG40810.4081No Hit
CCGGCATTCTCACTTCTAAGCGCTCCAGCCGTCCTCACGATCGACCTTCA38460.3846No Hit
GTAGGTCACTTGGTTTCGGGTCTACATCTGCTTACTCATTCGCCCTGTTC38230.3823No Hit
GGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCA37700.377No Hit
GTCACTTGGTTTCGGGTCTACATCTGCTTACTCATTCGCCCTGTTCAGAC36170.3617No Hit
GCCGGTTCATTCTACAAAAGGCACGCCATTACCCATTAACGGGCTTTGAC35940.3594No Hit
CTCCATCGCTTAAACAAAATAAACTAGTGCAGGAATCTCAACCTGCTTGT34120.3412No Hit
GGGTAGGTCACTTGGTTTCGGGTCTACATCTGCTTACTCATTCGCCCTGT33850.3385No Hit
CTCGCCGCTACTATGGGAATCGAGTTTTCTTTCTCTTCCTGCGGGTACTG31900.319No Hit
CGCCGGTTCATTCTACAAAAGGCACGCCATTACCCATTAACGGGCTTTGA31620.3162No Hit
CGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGA29740.2974No Hit
GTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCC29420.2942No Hit
GCCCCGGTATATTTTCGGCGCAGTGCCACTCGACTAGTGAGCTATTACGC28160.2816No Hit
GCCCTGTTCAGACTCGCTTTCGCTACGGCTCCGACTTTTCATCTTAACCT27790.2779No Hit
GCCGGTTCATTCTACAAAAGGCACGCCATTACCCGTTAACGGGCTTTGAC25400.254No Hit
GCCACATCCTTTTCCACTTAGCATAAATTTAGGGACCTTAACTGGTGATC24860.2486No Hit
CCTCCATCGCTTAAACAAAATAAACTAGTGCAGGAATCTCAACCTGCTTG24510.2451No Hit
CTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCG24380.24380000000000002No Hit
CTTGGTTTCGGGTCTACATCTGCTTACTCATTCGCCCTGTTCAGACTCGC24300.243No Hit
GTCATGGGTAGGTCACTTGGTTTCGGGTCTACATCTGCTTACTCATTCGC24290.2429No Hit
CTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAAT24210.2421No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC24150.2415TruSeq Adapter, Index 3 (100% over 50bp)
CCGGCATTCTCACTTCTAAGCGCTCCAGCCGTCCTCACGATCAACCTTCA23930.2393No Hit
GGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTC23650.2365No Hit
TGGGCCTATTCACTGCGGCTGACCTTGCGGTCAGCACCCCTTCTTCCGAA23320.2332No Hit
CTCCTGCGTCCCTCCATCGCTTAAACAAAATAAACTAGTGCAGGAATCTC23310.23310000000000003No Hit
CGCCGCTACTATGGGAATCGAGTTTTCTTTCTCTTCCTGCGGGTACTGAG22550.2255No Hit
CGCCGGTTCATTCTACAAAAGGCACGCCATTACCCGTTAACGGGCTTTGA21720.21719999999999998No Hit
CCGCCGGCCAGCTATGTATTCACTGACAAGCAATACACTGATGTGTACTG20810.2081No Hit
CCCCTTTCGACAATGGACCTTATCGCTCACTGTCTGACTCCCGGAGTAAG20700.207No Hit
CTTAACTGGTGATCTGGGCTGTTCCCCTTTCGACAATGGACCTTATCGCT20050.20049999999999998No Hit
CGGGTCTACATCTGCTTACTCATTCGCCCTGTTCAGACTCGCTTTCGCTA19950.19949999999999998No Hit
GTTCCCCTTTCGACAATGGACCTTATCGCTCACTGTCTGACTCCCGGAGT19950.19949999999999998No Hit
GCCTATTCACTGCGGCTGACCTTGCGGTCAGCACCCCTTCTTCCGAAGTT19900.199No Hit
CACTTGGTTTCGGGTCTACATCTGCTTACTCATTCGCCCTGTTCAGACTC19760.1976No Hit
TCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCG19660.1966No Hit
ATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCC18880.1888No Hit
CGGCATTCTCACTTCTAAGCGCTCCAGCCGTCCTCACGATCGACCTTCAA18790.1879No Hit
CTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCT18100.181No Hit
GACCTTAACTGGTGATCTGGGCTGTTCCCCTTTCGACAATGGACCTTATC18020.1802No Hit
CTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCTG17940.1794No Hit
CGCCGTACTCAGGATCCTGGACGGAGGGTTCGACGTTTCGCTTACAGGGC17740.1774No Hit
GCCGCTACTATGGGAATCGAGTTTTCTTTCTCTTCCTGCGGGTACTGAGA17320.1732No Hit
CTCGGTTTGGGCTCTTCCCACTTCGCTCGCCGCTACTATGGGAATCGAGT17250.1725No Hit
CGCCACATCCTTTTCCACTTAGCATAAATTTAGGGACCTTAACTGGTGAT17060.1706No Hit
AGGGACCTTAACTGGTGATCTGGGCTGTTCCCCTTTCGACAATGGACCTT17030.1703No Hit
CCGGTTCATTCTACAAAAGGCACGCCATTACCCATTAACGGGCTTTGACT16880.1688No Hit
GGTAGGTCACTTGGTTTCGGGTCTACATCTGCTTACTCATTCGCCCTGTT16710.1671No Hit
ACTTGGTTTCGGGTCTACATCTGCTTACTCATTCGCCCTGTTCAGACTCG16610.1661No Hit
GGCTGTTCCCCTTTCGACAATGGACCTTATCGCTCACTGTCTGACTCCCG16570.16570000000000001No Hit
CGGGCCTATTCACTGCGGCTGACCTTGCGGTCAGCACCCCTTCTTCCGAA16430.1643No Hit
CACGGTTTCAGGAACTGTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTT16100.161No Hit
GGGCTGTTCCCCTTTCGACAATGGACCTTATCGCTCACTGTCTGACTCCC15600.156No Hit
GTCCTCTTCTGCACTCAAGTTTCCCAGTTTCCGATGCGCTTCCTCGGTTA15590.1559No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC15390.1539TruSeq Adapter, Index 2 (100% over 50bp)
CTCGCCTTAGATCCCGACTAACCCTGGGAGGACGAGCCTTCCCCAGGAAA14990.14989999999999998No Hit
GGGACCTTAACTGGTGATCTGGGCTGTTCCCCTTTCGACAATGGACCTTA14970.1497No Hit
ATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGT14870.1487No Hit
CATGGGTAGGTCACTTGGTTTCGGGTCTACATCTGCTTACTCATTCGCCC14640.1464No Hit
CCCTACTGCTGCCTCCCGTAGGAGTTTGGGCCGTGTCTCAGTCCCAATGT14550.1455No Hit
CTTTCGACAATGGACCTTATCGCTCACTGTCTGACTCCCGGAGTAAGATC14510.1451No Hit
CGCTACTCATGCCGGCATTCTCACTTCTAAGCGCTCCAGCCGTCCTCACG14050.1405No Hit
CCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCAC13610.1361No Hit
CTGGTGATCTGGGCTGTTCCCCTTTCGACAATGGACCTTATCGCTCACTG13380.1338No Hit
GGGCCTATTCACTGCGGCTGACCTTGCGGTCAGCACCCCTTCTTCCGAAG13370.1337No Hit
GCCGCCGGCCAGCTATGTATTCACTGACAAGCAATACACTGATGTGTACT13240.13240000000000002No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG13180.1318TruSeq Adapter, Index 2 (100% over 49bp)
GCCGAGTTCCTTAACGAGAGTTCGCTCGCTCACCTGAGGATACTCTCCTC13100.131No Hit
CGTCCCTCCATCGCTTAAACAAAATAAACTAGTGCAGGAATCTCAACCTG12760.1276No Hit
CACACGGTTTCAGGAACTGTTTCACTCCCCTTCCGGGGTGCTTTTCACCT12750.1275No Hit
GTTTGGGCTCTTCCCACTTCGCTCGCCGCTACTATGGGAATCGAGTTTTC12710.1271No Hit
GTCATTTTGCCGAGTTCCTTAACGAGAGTTCGCTCGCTCACCTGAGGATA12570.1257No Hit
CCCTAGTTCAAACAGTGCTCTACCTCCATGATCCATCCTCCGAGGCTAAC12540.12539999999999998No Hit
GCCGTACTCAGGATCCTGGACGGAGGGTTCGACGTTTCGCTTACAGGGCT12390.12390000000000001No Hit
ACCTTAACTGGTGATCTGGGCTGTTCCCCTTTCGACAATGGACCTTATCG12270.1227No Hit
CCACTTAGCATAAATTTAGGGACCTTAACTGGTGATCTGGGCTGTTCCCC12180.12179999999999999No Hit
CACAGTTTCGGTATTATGCTTAGCCCCGGTATATTTTCGGCGCAGTGCCA12170.1217No Hit
GGTCATTTTGCCGAGTTCCTTAACGAGAGTTCGCTCGCTCACCTGAGGAT12160.12160000000000001No Hit
CCGGTTCATTCTACAAAAGGCACGCCATTACCCGTTAACGGGCTTTGACT12010.1201No Hit
CTGCCGCCGGCCAGCTATGTATTCACTGACAAGCAATACACTGATGTGTA11850.11850000000000001No Hit
GTCCAGTCCTACAACCCCGAGAAGCAAGCTTCTCGGTTTGGGCTTTTCCC11730.1173No Hit
CACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGA11710.1171No Hit
GGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGAC11710.1171No Hit
GTCTACATCTGCTTACTCATTCGCCCTGTTCAGACTCGCTTTCGCTACGG11280.11280000000000001No Hit
ACACGGTTTCAGGAACTGTTTCACTCCCCTTCCGGGGTGCTTTTCACCTT10930.1093No Hit
CGGCATTCTCACTTCTAAGCGCTCCAGCCGTCCTCACGATCAACCTTCAA10890.1089No Hit
CGGTATATTTTCGGCGCAGTGCCACTCGACTAGTGAGCTATTACGCACTC10770.1077No Hit
CCCGACTTTCGTCCCTGCTCGACTTGTCAGTCTCGCAGTCAAGCTCCCTT10750.1075No Hit
GCCTCCTGCGTCCCTCCATCGCTTAAACAAAATAAACTAGTGCAGGAATC10580.10579999999999999No Hit
CCCCGCGTCTGCCGCCGGCCAGCTATGTATTCACTGACAAGCAATACACT10480.1048No Hit
CTCCCCATCACAGCTTGTCCTTAAGACTGCAAGCATTTCACTCGCTGTCA10350.1035No Hit
GGGGGCTTCACACTTAGATGCTTTCAGCGTTTATCCCTGCCGTTCATAGC10070.10070000000000001No Hit
GCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCGGCGTGCTGATCCGC10050.1005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGAG3950.086.890045101
CTACAAT2150.084.51276101
AGGTGGA4450.083.98286100
GATGAGT9600.082.54421101
TCGGACC2350.081.6647595
CCCTAGG1000.080.8051295
CCTAGGT451.4897523E-978.5605496
GTTGTGG9650.075.840096101
GGCCCTG1800.075.75481100
TACTACA200.001626205775.7548196
TAGGTGG2250.074.0713699
GTTCTCT750.074.02692101
CGGGCCT2350.071.008221
TCCCTCG503.430614E-970.7044898
CCCACAC4400.070.015895
TACTTAG4050.068.584695
TCGACGC4500.068.4598995
ACTGGAC5100.068.28686101
GTCGAAG5400.068.23187101
TACCTCT451.3395584E-767.337698
Produced by FastQC (version 0.11.4)