FastQCFastQC Report
Sun 20 Dec 2015
QC.2.trimmed.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameQC.2.trimmed.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences977202
Sequences flagged as poor quality0
Sequence length50-93
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGCACTAGGAGCCGATGAAGGACGGAACTAATACCGATATGCTTC91630.9376771639845192No Hit
TGTGGGTAGCGGTGAAATTCCAAACGAACTTGGAGATAGCTGGTTCTCTC67700.6927943250218481No Hit
GTAGCGGTGAAATTCCAAACGAACTTGGAGATAGCTGGTTCTCTCCGAAA63720.6520657960176095No Hit
TGCCTTGGCACTAGGAGCCGATGAAGGACGGAACTAATACCGATATGCTT53290.5453324901095168No Hit
TCAGCTGGAAAGCTGCGCCACAGAAGGTGAAAGCCCTGTAAGCGAAACGT37250.3811903782431882No Hit
GCACCCCGGAAGGGGAGTGAAACAGTTCCTGAAACCGTGTGCCTACAATT35980.3681940888373131No Hit
TGAGCGATAAGGTCCATTGTCGAAAGGGGAACAGCCCAGATCACCAGTTA35920.367580090912626No Hit
GATGGATCATGGAGGTAGAGCACTGTTTGAACTAGGGGCCCATCAAGGGT32060.3280795577577615No Hit
TGGAGTTACCAAAGTCCGACGTAGTCGAAGTCAGCTGGAAAGCTGCGCCA31410.32142791357365214No Hit
TCACCAGTTAAGGTCCCTAAATTTATGCTAAGTGGAAAAGGATGTGGCGT28850.29523066878700616No Hit
ATTGGAGTTACCAAAGTCCGACGTAGTCGAAGTCAGCTGGAAAGCTGCGC28020.28673703082883584No Hit
AAGCGAAACGTCGAACCCTCCGTCCAGGATCCTGAGTACGGCGGAACACG27670.28315537626816156No Hit
ACATTGGAGTTACCAAAGTCCGACGTAGTCGAAGTCAGCTGGAAAGCTGC27030.27660606507150004No Hit
TCTCGGGGTTGTAGGACTGGACATTGGAGTTACCAAAGTCCGACGTAGTC26000.266065767364373No Hit
CGAAGTGGGAAGAGCCCAAACCGAGAAGCTTGCTTCTCGGGGTTGTAGGA25070.25654879953172427No Hit
CTTACTCCGGGAGTCAGACAGTGAGCGATAAGGTCCATTGTCGAAAGGGG24920.25501380472000673No Hit
TGTCGAAAGGGGAACAGCCCAGATCACCAGTTAAGGTCCCTAAATTTATG24280.2484644935233452No Hit
CGAGAAGCTTGCTTCTCGGGGTTGTAGGACTGGACATTGGAGTTACCAAA23480.24027785452751835No Hit
GGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGAACTAATACCGAT23250.23792419581621813No Hit
CTCTCCGAAATAGCTTTAGGGTTAGCCTCGGAGGATGGATCATGGAGGTA23060.23597986905470927No Hit
CGACGTAGTCGAAGTCAGCTGGAAAGCTGCGCCACAGAAGGTGAAAGCCC23040.2357752030798136No Hit
GGGTAGCGGTGAAATTCCAAACGAACTTGGAGATAGCTGGTTCTCTCCGA22610.23137488461955663No Hit
GTGGGTAGCGGTGAAATTCCAAACGAACTTGGAGATAGCTGGTTCTCTCC21970.22482557342289516No Hit
ACTCCGGGAGTCAGACAGTGAGCGATAAGGTCCATTGTCGAAAGGGGAAC21300.21796926326389018No Hit
CCTTGGCACTAGGAGCCGATGAAGGACGGAACTAATACCGATATGCTTCG20810.21295494687894623No Hit
TGAACTAGGGGCCCATCAAGGGTTACTGAATTCAGATAAACTCCGAATAC20520.20998729024295898No Hit
GAACCAGTACCGTGAGGGAAAGGTGAAAAGCACCCCGGAAGGGGAGTGAA19800.20261931514671483No Hit
GTAGGACTGGACATTGGAGTTACCAAAGTCCGACGTAGTCGAAGTCAGCT18740.1917720184772442No Hit
CTTGGCACTAGGAGCCGATGAAGGACGGAACTAATACCGATATGCTTCGG18680.1911580205525572No Hit
TTGGAGTTACCAAAGTCCGACGTAGTCGAAGTCAGCTGGAAAGCTGCGCC18150.18573437221782188No Hit
TGAATTCAGATAAACTCCGAATACCATCGATCTTACTCCGGGAGTCAGAC17990.18409704441865654No Hit
TCCGTCGGAATCCGGGAGGACCATCTCCCAAGGCTAAATACTCCCTAGTG17240.17642207036006885No Hit
GAGCGATAAGGTCCATTGTCGAAAGGGGAACAGCCCAGATCACCAGTTAA16920.1731474147617381No Hit
GGAGTTACCAAAGTCCGACGTAGTCGAAGTCAGCTGGAAAGCTGCGCCAC16680.17069142306299004No Hit
CTTGCTTCTCGGGGTTGTAGGACTGGACATTGGAGTTACCAAAGTCCGAC16460.16844009733913765No Hit
AGTCGAGTGGCACTGCGCCGAAAATATACCGGGGCTAAGCATAATACCGA15840.16209545211737186No Hit
ATAGCTTTAGGGTTAGCCTCGGAGGATGGATCATGGAGGTAGAGCACTGT15800.1616861201675805No Hit
GAAGTCAGCTGGAAAGCTGCGCCACAGAAGGTGAAAGCCCTGTAAGCGAA15780.16148145419268484No Hit
TTACTCCGGGAGTCAGACAGTGAGCGATAAGGTCCATTGTCGAAAGGGGA15510.15871846353159327No Hit
GGGAAGAGCCCAAACCGAGAAGCTTGCTTCTCGGGGTTGTAGGACTGGAC15130.1548298100085755No Hit
GGAGGCTAAGGAAAGCGCACGGCTGGAAATGTGCGCCCAAGCAGCAAGTC14640.14981549362363156No Hit
TCAGACAGTGAGCGATAAGGTCCATTGTCGAAAGGGGAACAGCCCAGATC14610.14950849466128804No Hit
CTAATACCGATATGCTTCGGGGAGCTATAAGTAAGCTTTGATCCGGAGAT14610.14950849466128804No Hit
ATGCCTTGGCACTAGGAGCCGATGAAGGACGGAACTAATACCGATATGCT14400.1473595019248835No Hit
ATCAGTGAGAATCTGATCCACCGTATGACTAAGGTTTCCTGGGGAAGGCT14180.1451081762010311No Hit
GTCCATTGTCGAAAGGGGAACAGCCCAGATCACCAGTTAAGGTCCCTAAA14030.14357318138931358No Hit
CTAGGAGCCGATGAAGGACGGAACTAATACCGATATGCTTCGGGGAGCTA13820.14142418865290904No Hit
TTCCGTCGGAATCCGGGAGGACCATCTCCCAAGGCTAAATACTCCCTAGT13490.13804720006713045No Hit
CCGAGAAGCTTGCTTCTCGGGGTTGTAGGACTGGACATTGGAGTTACCAA13450.1376378681173391No Hit
AGCGGTGAAATTCCAAACGAACTTGGAGATAGCTGGTTCTCTCCGAAATA13390.13702387019265208No Hit
AGGAGGCTAAGGAAAGCGCACGGCTGGAAATGTGCGCCCAAGCAGCAAGT13380.13692153720520425No Hit
TGAGTAGCGAAAGATCAGTGAGAATCTGATCCACCGTATGACTAAGGTTT13350.13661453824286074No Hit
GAAGTGGGAAGAGCCCAAACCGAGAAGCTTGCTTCTCGGGGTTGTAGGAC13200.1350795434311432No Hit
GACCATCTCCCAAGGCTAAATACTCCCTAGTGACCGATAGTGAACCAGTA12770.13067922497088627No Hit
TGAAAAGCACCCCGGAAGGGGAGTGAAACAGTTCCTGAAACCGTGTGCCT12660.12955356210896007No Hit
TGCACAGACAACTAGGATGTTGGCTCAGAAGCAGCCACCATTTAAAGAGT12650.12945122912151225No Hit
TCCAAACGAACTTGGAGATAGCTGGTTCTCTCCGAAATAGCTTTAGGGTT12620.12914423015916873No Hit
GTAGTCGAAGTCAGCTGGAAAGCTGCGCCACAGAAGGTGAAAGCCCTGTA12500.12791623430979468No Hit
TCCCTAAATTTATGCTAAGTGGAAAAGGATGTGGCGTTGCACAGACAACT12480.12771156833489902No Hit
CTGTGATGGGGAGCGAAATTATAGTAGCGAAGTTCCTGATGTCACACTGC12380.12668823846042068No Hit
GTCGAACCCTCCGTCCAGGATCCTGAGTACGGCGGAACACGTGAAATTCC12340.1262789065106293No Hit
ATTCCAAACGAACTTGGAGATAGCTGGTTCTCTCCGAAATAGCTTTAGGG12230.12515324364870312No Hit
GGAGGTAGAGCACTGTTTGAACTAGGGGCCCATCAAGGGTTACTGAATTC12180.12464157871146396No Hit
CAGGATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGAGTTCT12130.12412991377422478No Hit
CTCGGGGTTGTAGGACTGGACATTGGAGTTACCAAAGTCCGACGTAGTCG12080.1236182488369856No Hit
TATGCTAAGTGGAAAAGGATGTGGCGTTGCACAGACAACTAGGATGTTGG11940.1221855870127159No Hit
TATAGTAGCGAAGTTCCTGATGTCACACTGCCAAGAAAAGCTTCTAGTGA11840.12116225713823753No Hit
AGTCAGACAGTGAGCGATAAGGTCCATTGTCGAAAGGGGAACAGCCCAGA11820.12095759116334187No Hit
TTCCGAATGGGGGAACCCAGTACACATCAGTGTATTGCTTGTCAGTGAAT11770.12044592622610269No Hit
TGAACCAGTACCGTGAGGGAAAGGTGAAAAGCACCCCGGAAGGGGAGTGA11610.11880859842693732No Hit
CACCAGTTAAGGTCCCTAAATTTATGCTAAGTGGAAAAGGATGTGGCGTT11590.11860393245204165No Hit
GTCGATGCAGGAACCGAGCATTACGATCCTTGTCTAGCATTACCAAGACG11540.11809226751480248No Hit
AGTACCGTGAGGGAAAGGTGAAAAGCACCCCGGAAGGGGAGTGAAACAGT11330.11594327477839793No Hit
GACCGATAGTGAACCAGTACCGTGAGGGAAAGGTGAAAAGCACCCCGGAA11080.11338495009220201No Hit
CAAGGCTAAATACTCCCTAGTGACCGATAGTGAACCAGTACCGTGAGGGA10980.11236162021772365No Hit
CGTGAGGACGGCTGGAGCGCTTAGAAGTGAGAATGCCGGCATGAGTAGCG10920.11174762229303666No Hit
CCATCTCCCAAGGCTAAATACTCCCTAGTGACCGATAGTGAACCAGTACC10910.11164528930558881No Hit
CTAAATACTCCCTAGTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGT10850.1110312913809018No Hit
GGAAAAGGATGTGGCGTTGCACAGACAACTAGGATGTTGGCTCAGAAGCA10830.11082662540600613No Hit
CGGTGAAATTCCAAACGAACTTGGAGATAGCTGGTTCTCTCCGAAATAGC10780.11031496046876695No Hit
GCAGCCACCATTTAAAGAGTGCGTAATAGCTCACTAGTCGAGTGGCACTG10610.10857529968215374No Hit
GTTAGCCTCGGAGGATGGATCATGGAGGTAGAGCACTGTTTGAACTAGGG10570.10816596773236241No Hit
TTCCAAACGAACTTGGAGATAGCTGGTTCTCTCCGAAATAGCTTTAGGGT10500.10744963682022754No Hit
GGTCCATTGTCGAAAGGGGAACAGCCCAGATCACCAGTTAAGGTCCCTAA10360.10601697499595786No Hit
TGAGTACGGCGGAACACGTGAAATTCCGTCGGAATCCGGGAGGACCATCT10180.1041749812218968No Hit
CATTGGAGTTACCAAAGTCCGACGTAGTCGAAGTCAGCTGGAAAGCTGCG10010.10243532043528358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG2250.076.2279
TCGTGTA2800.061.2538388
AGCGTCG2850.060.179214
GAGCGTC2900.059.141643
GTCGTGT3300.053.2722787
GCGTCGT3700.045.19545
GGTGGAT5300.040.450651
AAGAGCG4250.040.3554731
GTGTAGA2300.039.1491922-23
TGTAGGG2400.038.41126310-11
CACCCTA1600.037.5179751
GTGGATG6200.035.961932
GTAGATC2750.035.8613424-25
AGTGTAG2350.035.57935722-23
TAGATCT2650.034.7875626-27
TGCCTCG652.423868E-432.9828388
CCCTAAC1700.032.7888223
GCCTTGG26200.032.2400931
GTGGGTA19600.031.5019742
CAGGATG3900.030.7839811
Produced by FastQC (version 0.11.4)