FastQCFastQC Report
Mon 12 Feb 2018
Trimmomatic_on_DRR024501sub_1_fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTrimmomatic_on_DRR024501sub_1_fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264315
Sequences flagged as poor quality0
Sequence length4-251
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGCA850.0026148886202.02339245
CGCCTAC600.00652373218.743113125-129
ACAAACG3850.00980580316.010393235-239
AGTCACG11700.001151201714.676914245
CTGTATA1700.004667707711.429926165-169
TCGTATG4950.00144631448.0408325205-209
TCCAGTC12758.9410885E-46.734113245
CGTCTGA13101.06586675E-46.5541945245
TCACGGA11103.0042918E-46.4662905240-244
GTCACGG11505.4338045E-46.429625230-234
CACGGAT11101.4084224E-46.0925794215-219
CAGTCAC13201.7039034E-55.7691493210-214
CGGATGT8900.00123714575.5723147215-219
CTCCAGT12600.0048153495.3573422225-229
GAACTCC12851.7536887E-45.2656503205-209
ACGTCTG12900.00107558115.242452215-219
CCAGTCA14102.8173352E-45.1344595220-224
Produced by FastQC (version 0.11.5)