FastQCFastQC Report
Tue 29 Dec 2015
QC.2.trimmed.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameQC.2.trimmed.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297633
Sequences flagged as poor quality0
Sequence length50-251
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC3000.0123.13217
CTCGGTG3450.0113.921791
GTCGCCG3800.097.209568
TCGCCGT3700.093.395769
TGGTCGC4700.078.594966
TCGGTGG5150.076.3165362
GTGGTCG5400.072.820075
GGTGGTC5850.065.148744
CGGTGGT6100.062.4787143
CGGAAGG750.007319744751.809498245
TGTAGGG3354.7439244E-934.79743245
TAGGGAA2509.175565E-625.904749245
CGCCGTA3000.024.63139210-14
ACCTCAT2900.003830790520.5448465
CCGTATC3600.020.52616110-14
GCCGTAT3950.019.3108510-14
GTGTAGG3550.018.970413240-244
AGGGAAA4300.001077584918.073082245
CGTGTAG3300.018.052847240-244
CGTATCA4900.015.5669110-14
Produced by FastQC (version 0.11.4)