FastQCFastQC Report
Tue 29 Dec 2015
DRR024501sub_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024501sub_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences300000
Sequences flagged as poor quality0
Sequence length251
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTG1350.002027568436.27465245
AGATCTG1650.00541865229.67926245
AGCAGCG3000.003864309820.5139888
GTACCTC809.7034924E-418.36526575-79
AGGCGAA1850.001842546410.58509735-39
CAACTAG6250.0087863975.0912623195-199
Produced by FastQC (version 0.11.4)