スタッフ

教授 大西 康夫 2010年12月26日 醗酵学研究室教授室にて

教授 大西 康夫
[Professor Yasuo OHNISHI, Ph.D.]
ayasuo _at_ mail.ecc.u-tokyo.ac.jp
(スパムメール防止のため、@を_at_に置き換えています)

学歴

1987年3月 大阪府立 天王寺高等学校 卒業
1987年4月 東京大学教養学部理科二類 入学
1989年4月 東京大学農学部農芸化学科 進学
1991年3月 同上 卒業
1991年4月 東京大学大学院農学系研究科応用生命工学専攻 修士課程 入学
1993年3月 同上 修了
1993年4月 東京大学大学院農学生命科学研究科応用生命工学専攻 博士課程 進学
1996年3月 同上 修了  博士(農学)の学位取得

職歴

1995年4月 日本学術振興会特別研究員DC2 (東京大学)
1996年4月 日本学術振興会特別研究員PD【資格変更】(東京大学)
1997年4月 日本学術振興会特別研究員PD【再採用】(東京大学)
1997年8月 東京大学大学院農学生命科学研究科 助手
2002年8月 東京大学大学院農学生命科学研究科 助教授
2007年4月 東京大学大学院農学生命科学研究科 准教授
2010年7月 東京大学大学院農学生命科学研究科 教授 (現職)

受賞

2002年5月 日本放線菌学会 浜田賞
「放線菌の二次代謝・形態分化に関与するA-ファクター制御カスケードに関する研究」
2003年3月 日本農芸化学会 農芸化学奨励賞
「放線菌の二次代謝・形態分化に関する分子遺伝学的研究」
2007年11月 財団法人農学会 日本農学進歩賞
「放線菌の遺伝子発現制御カスケードと新規生合成酵素に関する研究」
2008年4月 科学技術分野の文部科学大臣表彰 若手科学者賞
「放線菌の遺伝子発現制御機構と新規生合成酵素の研究」
2014年2月 日本学術振興会賞
「放線菌の遺伝子発現制御機構と二次代謝産物生合成に関する研究」

学会等での活動

2001-2003 日本農芸化学会  関東支部幹事
2004-2005 日本農芸化学会  産学官若手交流会(さんわか)世話人
2008-2012 日本放線菌学会 理事
2009- 日本ゲノム微生物学会 幹事
2010-2013 知的クラスター形成に向けた研究拠点構築事業
「沖縄生物資源の活用促進に向けた研究基盤の構築」 推進委員
2010- JST先端的低炭素化技術開発事業(ALCA)
推進委員会分科会委員
2010- 発酵と代謝研究会 幹事
2012- Microbiology (SGM) 誌 Associate Editor
2013- 日本農芸化学会 理事(会計)、財務委員会委員
2013- 応用微生物学・分子細胞生物学研究奨励会 理事
2013- 日本微生物学連盟 理事(日本農芸化学会より)

学内での活動

2012.4-2014.3 学部委員
2013.4- 東京大学生命科学ネットワーク幹事
2014.4- 応用生命工学専攻長

研究業績(〜2014.3)

【原著論文】

  1. Hiroya Tomita, Yohei Katsuyama, Hiromichi Minami, and Yasuo Ohnishi (2017) Identification and characterization of a bacterial cytochrome P450 monooxygenase catalyzing the 3-nitration of tyrosine in rufomycin biosynthesis J. Biol. Chem. in press
  2. Hideo Kawaguchi, Yohei Katsuyama, Du Danyao, Prihardi Kahar, Sachiko Nakamura-Tsuruta, Hiroshi Teramura, Keiko Wakai, Kumiko Yoshihara, Hiromichi Minami, Chiaki Ogino, Yasuo Ohnishi, Ahikiko Kondo (2017) Caffeic acid production by simultaneous saccharification and fermentation of kraft pulp using recombinant Escherichia coli. Appl. Microbiol. Biotechnol. 101, 5279-5290
  3. Yoshihiro Mouri, Kenji Konishi, Azusa Fujita, Takeaki Tezuka, Yasuo Ohnishi (2017) Regulation of sporangium formation by BldD in the rare actinomycete Actinoplanes missouriensis. J. Bacteriol., 199, pii: e00840-16.
  4. Yoshihiro Mouri, Kenji Konishi, Azusa Fujita, Takeaki Tezuka, Yasuo Ohnishi (2017) Regulation of sporangium formation by BldD in the rare actinomycete Actinoplanes missouriensis. J. Bacteriol., in press
  5. Yuji Ishigaki, Genki Akanuma, Minoru Yoshida, Sueharu Horinouchi, Saori Kosono, Yasuo Ohnishi (2016) Protein acetylation involved in streptomycin biosynthesis in Streptomyces griseus. J. Proteomics S1874-3919, 30523.
  6. Takaaki Taguchi, Takayoshi Awakawa, Yukitaka Nishihara, Michiho Kawamura, Yasuo Ohnishi, Koji Ichinose. (2016) Bifunctionality of ActIV as a Cyclase-Thioesterase Revealed by In Vitro Reconstitution of Actinorhodin Biosynthesis in Streptomyces coelicolor A3(2). Chembiochem, 18, 316-323.
  7. Mai Tsujimoto, Ayako Yoshida, Tetsu Shimizu, Takeo Tomita, Yasuo Ohnishi, Tomohisa Kuzuyama, Makoto Nishiyama. (2016) Aspartate kinase involved in 4-hydroxy-3-nitrosobenzamide biosynthesis in Streptomyces murayamaensis. Biosci. Biotechnol. Biochem., 2, 1-9.
  8. Akihiro Ninomiya, Yohei Katsuyama, Takefumi Kuranaga, Masayuki Miyazaki, Yuichi Nogi, Shigeru Okada, Toshiyuki Wakimoto, Yasuo Ohnishi, Shigeki Matsunaga, Kentaro Takada. (2016) Biosynthetic Gene Cluster for Surugamide A Encompasses an Unrelated Decapeptide, Surugamide F. ChemBioChem, 17, 1709-1712.
  9. Moon-Sun Jang, Yoshihiro Mouri, Kaoru Uchida, Shin-Ichi Aizawa, Masayuki Hayakawa, Nobuyuki Fujita, Takeaki Tezuka, and Yasuo Ohnishi (2016) Genetic and transcriptional analysis of the flagellar gene cluster in Actinoplanes missouriensis. J. Bacteriol., 198, 2219-2227.
  10. Danyao Du, Yohei Katsuyama, Hiroyasu Onaka, Manabu Fujie, Noriyuki Satoh, Kazuo Shin-ya, and Yasuo Ohnishi (2016) Production of a novel amide-containing polyene by activating a cryptic biosynthetic gene cluster in Streptomyces sp. MSC090213JE08. Chembiochem, 17, 1464-1471.
  11. Yohei Katsuyama, Kaoru Sone, Ryutaro Satou, Miho Izumikawa, Motoki Takagi, Manabu Fujie, Noriyuki Satoh, Kazuo Shin-ya, Yasuo Ohnishi (2016) Involvement of the Baeyer–Villiger Monooxygenase IfnQ in the Biosynthesis of Isofuranonaphthoquinone Scaffold of JBIR-76 and -77. Chembiochem, 17, 1021-1028.
  12. Yoshinori Sugai, Yohei Katsuyama, Yasuo Ohnishi (2016) Nat. Chem. Biol., 17, 1021-1028.
  13. Hideo Kawaguchia, Kengo Sasaki, Kouji Uematsu, Yota Tsuge, Hiroshi Teramura, Naoko Okai, Sachiko Nakamura-Tsuruta, Yohei Katsuyama, Yoshinori Sugai, Yasuo Ohnishi, Ko Hirano, Takashi Sazuka, Chiaki Ogino, Akihiko Kondo (2015) Bioresour. Technol. 198, 410-417.
  14. Shoto Ohno, Yohei Katsuyama, Yuka Tajima, Miho Izumikawa, Motoki Takagi, Manabu Fujie, Noriyuki Satoh, Kazuo Shin-ya, Yasuo Ohnishi (2015) Identification and Characterization of the Streptazone E Biosynthetic Gene Cluster in Streptomyces sp. MSC090213JE08. Chembiochem [Epub ahead of print]
  15. Motoki Hojo, Ai Omi, Gen Hamanaka, Kazutoshi Shindo, Atsuko Shimada, Mariko Kondo, Takanori Narita, Masato Kiyomoto, Yohei Katsuyama, Yasuo Ohnishi, Naoki Irie, Hiroyuki Takeda. (2015) Unexpected link between polyketide synthase and calcium carbonate biomineralization. Zoological Letters, 1: 3.
  16. Moon-Sun Jang, Azusa Fujita, Satomi Ikawa, Keitaro Hanawa, Hideki Yamamura, Tomohiko Tamura, Masayuki Hayakawa, Takeaki Tezuka, Yasuo Ohnishi. (2015) Isolation of a novel plasmid from Couchioplanes caeruleus and construction of two plasmid vectors for gene expression in Actinoplanes missouriensis. Plasmid, 77, 32-38.
  17. Adeline Muliandi, Yohei Katsuyama, Kaoru Sone, Miho Izumikawa, Tomohiro Moriya, Junko Hashimoto, Ikuko Kozone, Motoki Takagi, Kazuo Shin-ya, Yasuo Ohnishi. (2014) Biosynthesis of the 4-methyloxazoline-containing nonribosomal peptides, JBIR-34 and -35, in Streptomyces sp. Sp080513GE-23. Chem. Biol., 21, 923-934.
  18. Takeaki Tezuka, Yasuo Ohnishi. (2014) Two glycine riboswitches activate the glycine cleavage system essential for glycine detoxification in Streptomyces griseus. J. Bacteriol., 196, 1369-1376.
  19. Takuya Makino, Yohei Katsuyama, Toshihiko Otomatsu, Norihiko Misawa, Yasuo Ohnishi. (2014) Regio- and stereo-specific hydroxylation of various steroids at the 16α position of the D-ring by the Streptomyces griseus cytochrome P450 CYP154C3. Appl. Environ. Microbiol., 80, 1371-1379.
  20. Ryutaro Satou, Miho Izumikawa, Yohei Katsuyama, Misato Matsui, Motoki Takagi, Kazuo Shin-ya, Yasuo Ohnishi. (2014) Isolation, structural elucidation and biosynthesis of 3-hydroxy-6-dimethylallylindolin-2-one, a novel prenylated indole derivative from Actinoplanes missouriensis. J. Antibiot., 67, 231-236.
  21. Ryutaro Satou, Akimasa Miyanaga, Hiroki Ozawa, Nobutaka Funa, Yohei Katsuyama, Ken-ichi Miyazono, Masaru Tanokura, Yasuo Ohnishi, Sueharu Horinouchi. (2013) Structural basis for cyclization specificity of two Azotobacter type III polyketide synthases: a single amino acid substitution reverses their cyclization specificity. J. Biol. Chem., 288, 34146-34157.
  22. Ming Dong Yao, Jun Ohtsuka, Koji Nagata, Ken-ichi Miyazono, Yuehua Zhi, Yasuo Ohnishi, Masaru Tanokura. (2013) Complex structure of the DNA-binding domain of AdpA, the global transcription factor in Streptomyces griseus, and a target duplex DNA reveals the structural basis of its tolerant DNA sequence specificity. J. Biol. Chem., 288, 31019-31029.
  23. Geoffrey P. Horsman, Anna Lechner, Yasuo Ohnishi, Bradley S. Moore, Ben Shen. (2013) Predictive model for epoxide hydrolase-generated stereochemistry in the biosynthesis of nine-membered enediyne antitumor antibiotics. Biochemistry, 52, 5217–5224.
  24. Takayoshi Awakawa, Yoshinori Sugai, Kanae Otsutomo, Shukun Ren, Shinji Masuda, Yohei Katsuyama, Sueharu Horinouchi, Yasuo Ohnishi. (2013) 4-Hydroxy-3-methyl-6-(1-methyl-2-oxoalkyl)pyran-2-one synthesis by a type III polyketide synthase from Rhodospirillum centenum. Chembiochem 14, 1006-1013.
  25. Hiroshi Otani, Akiyoshi Higo, Hideaki Nanamiya, Sueharu Horinouchi, Yasuo Ohnishi. (2013) An alternative sigma factor governs the principal sigma factor in Streptomyces griseus. Mol. Microbiol., 87, 1223-1236.
  26. Hideki Yamamura, Yasuo Ohnishi, Jun Ishikawa, Natsuko Ichikawa, Haruo Ikeda, Mitsuo Sekine, Takeshi Harada, Sueharu Horinouchi, Misa Otoguro, Tomohiko Tamura, Ken-ichiro Suzuki, Yasutaka Hoshino, Akira Arisawa, Youji Nakagawa, Nobuyuki Fujita, Masayuki Hayakawa. (2012) Complete genome sequence of the motile actinomycete Actinoplanes missouriensis 431T (= NBRC 102363T). Stand. Genomic. Sci., 7, 294-303.
  27. Genki Akanuma, Hideaki Nanamiya, Yoshihiro Mouri, Morio Ishizuka, Yasuo Ohnishi. (2012) Proteomic analysis of the Streptomyces griseus ribosomal fraction. Biosci. Biotechnol. Biochem., 76, 2267-2274.
  28. Ryouhei Nomoto, Takeaki Tezuka, Kenichi Miyazono, Masaru Tanokura, Sueharu Horinouchi, Yasuo Ohnishi. (2012) Purification, crystallization and preliminary X-ray analysis of SGR6054, a Streptomyces homologue of the mycobacterial integration host factor mIHF. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun.. 68, 1085-1088.
  29. Chiaki Nakano, Takeaki Tezuka, Sueharu Horinouchi, Yasuo Ohnishi. (2012) Identification of the SGR6065 gene product as a sesquiterpene cyclase involved in (+)-epicubenol biosynthesis in Streptomyces griseus. J. Antibiot.. 65, 551-558.
  30. Ming Dong Yao, Ken-ichi Miyazono, Jun Ohtsuka, Setsu Hirano, Koji Nagata, Sueharu Horinouchi, Yasuo Ohnishi, Masaru Tanokura. (2012) Purification, crystallization and preliminary X-ray analysis of the DNA-binding domain of AdpA, the central transcription factor in the A-factor regulatory cascade in the filamentous bacterium Streptomyces griseus, in complex with a duplex DNA. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun., 68, 946-949.
  31. Jake W. Ginsbacha, Matthew T. Kieber-Emmonsa, Ryohei Nomoto, Akio Noguchi, Yasuo Ohnishi, and Edward I. Solomona. (2012) Structure/function correlations among coupled binuclear copper proteins through spectroscopic and reactivity studies of NspF. Proc. Natl. Acad. Sci. U. S. A., 109, 10793-10797.
  32. Akiyoshi Higo, Hirofumi Hara, Sueharu Horinouchi, Yasuo Ohnishi. (2012) Genome-wide distribution of AdpA, a global regulator for secondary metabolism and morphological differentiation in Streptomyces, revealed the extent and complexity of the AdpA regulatory network. DNA Res. 19, 259-273.
  33. Yukihide Sato, Sueharu Horinouchi, Yasuo Ohnishi. (2012) Characterization of a thermostable adenosine 5’-monophosphate deaminase gene in Streptomyces murinus. 
J. Gen. Appl. Microbiol., 58, 65-70.
  34. Chiaki Nakano, Nobutaka Funa, Yasuo Ohnishi, Sueharu Horinouchi. (2012)
The O-methyltransferase SrsB catalyzes the decarboxylative methylation of alkylresorcylic acid during phenolic lipid biosynthesis by Streptomyces griseus. 
J. Bacteriol., 194, 1544-1551.
  35. Ken-ichi Miyazono, Nobumitsu Tabei, Sho Morita, Yasuo Ohnishi, Sueharu Horinouchi, Masaru Tanokura. (2012) Substrate recognition mechanism and substrate-dependent conformational changes of an ROK family glucokinase from Streptomyces griseus. 
J. Bacteriol., 194, 607-616.
  36. Miho Izumikawa, Ryutaro Satou, Keiichiro Motohashi, Aya Nagai, Yasuo Ohnishi, Motoki Takagi, Kazuo Shin-ya. (2012) Naphthoquinone-like polyketide isolated from Streptomyces sp. RI-77 and its predicted biosynthetic pathway. J. Nat. Prod., 74, 2588-2591.
  37. Chiaki Nakano, Hyo-Kyoung Kim, Yasuo Ohnishi. (2012) Identification and characterization of the linalool/nerolidol synthase from Streptomyces clavuligerus. 
Chembiochem, 12, 2403-2407.
  38. Chiaki Nakano, Fumitaka Kudo, Tadashi Eguchi, Yasuo Ohnishi. (2011) Genome mining reveals two novel bacterial sesquiterpene cyclases: (–)-germacradien-4-ol and (–)-epi-alpha-bisabolol synthases from Streptomyces citricolor. 
Chembiochem, 12, 2271-2275.
  39. Akiyoshi Higo, Sueharu Horinouchi, Yasuo Ohnishi. (2011) Strict regulation of morphological differentiation and secondary metabolism by a positive feedback loop between two global regulators AdpA and BldA in Streptomyces griseus. 

Mol. Microbiol., 81, 1607-1622.
  40. Takayuki Hayashi, Yuta Kitamura, Nobutaka Funa, Yasuo Ohnishi, Sueharu Horinouchi. (2011) Fatty acyl-AMP ligase involvement in the production of alkylresorcylic acid by a Myxococcus xanthus type III polyketide synthase. 
Chembiochem, 12, 2166-2176.
  41. Ken-ichi Miyazono, Nobumitsu Tabei, Kazuya Marushima, Yasuo Ohnishi, Sueharu Horinouchi, Masaru Tanokura. (2011) Purification, crystallization and preliminary X-ray analysis of glucokinase from Streptomyces griseus in complex with glucose. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun., 67, 914–916.
  42. Chiaki Nakano, Hyo-Kyoung Kim, Yasuo Ohnishi. (2011) Identification of the first bacterial monoterpene cyclase, a 1,8-cineole synthase, that catalyzes the direct conversion of geranyl diphosphate. Chembiochem, 12, 1988-1991.
  43. Chiaki Nakano, Sueharu Horinouchi, Yasuo Ohnishi. (2011) Characterization of a novel sesquiterpene cyclase involved in (+)-caryolan-1-ol biosynthesis in Streptomyces griseus. J. Biol. Chem., 286, 27980-27987.
  44. Tomojiro Koide, Yasuo Ohnishi, Sueharu Horinouchi. (2011) Characterization of recombinant alpha-amylases from Oryza sativa. Biosci. Biotechnol. Biochem., 75, 793-796.
  45. Kaoru Uchida, Moon-Sun Jang, Yasuo Ohnishi, Sueharu Horinouchi, Masayuki Hayakawa, Nobuyuki Fujita, Shin-Ichi Aizawa. (2011) Characterization of spore flagella in Actinoplanes missouriensis. 
Appl. Environ. Microbiol., 77, 2559-2562.
  46. Takayoshi Awakawa, Nobuyuki Fujita, Masayuki Hayakawa, Yasuo Ohnishi, Sueharu Horinouchi. (2011) Characterization of the biosynthesis gene cluster for alkyl-O-dihydrogeranyl-methoxyhydroquinones in Actinoplanes missouriensis. Chembiochem, 12, 439-448.
  47. Genki Akanuma, Masayoshi Ueki, Morio Ishizuka, Yasuo Ohnishi, Sueharu Horinouchi. (2011) Control of aerial mycelium formation by the BldK oligopeptide ABC transporter in Streptomyces griseus. FEMS Microbiol. Lett., 315, 54-62.
  48. Yohei Katsuyama, Ken-ichi Miyazono, Masaru Tanokura, Yasuo Ohnishi, Sueharu Horinouchi. (2011) Structural and biochemical elucidation of mechanism for decarboxylative condensation of beta-keto acid by curcumin synthase. J. Biol. Chem., 286, 6659-6668.
  49. Ken-ichi Miyazono, Jounghyun Um, Fabiana Lica Imai, Yohei Katsuyama, Yasuo Ohnishi, Sueharu Horinouchi, Masaru Tanokura. (2011) Crystal structure of curcuminoid synthase CUS from Oryza sativa. Proteins, 79, 669-673.
  50. Akio Noguchi, Sueharu Horinouchi, Yasuo Ohnishi. (2011) Substrate specificity of benzamide synthetase involved in 4-hydroxy-3-nitrosobenzamide biosynthesis. J. Antibiot., 64, 93-96.
  51. Takeaki Tezuka, Yasuo Ohnishi, Sueharu Horinouchi (2010) Conditional effect of the deletion of eshA on streptomycin production in Streptomyces griseus IFO13350. Actinomycetologica, 24, 45-50.
  52. Natsuko Ichikawa, Akio Oguchi, Haruo Ikeda, Jun Ishikawa, Shigeru Kitani, Yumi Watanabe, Sanae Nakamura, Yoko Katano, Emi Kishi, Machi Sasagawa, Akiho Ankai, Shigehiro Fukui, Yoshimi Hashimoto, Sachi Kamata, Misa Otoguro, Satoshi Tanikawa, Takuya Nihira, Sueharu Horinouchi, Yasuo Ohnishi, Masayuki Hayakawa, Tomohisa Kuzuyama, Akira Arisawa, Fumiki Nomoto, Hiromi Miura, Yoko Takahashi, Nobuyuki Fujita. (2010) Genome sequence of Kitasatospora setae NBRC 14216T: an evolutionary snapshot of the family Streptomycetaceae. DNA Res., 17, 393–406.
  53. Yohei Katsuyama, Yasuo Ohnishi, Sueharu Horinouchi. (2010) Production of dehydrogingerdione derivatives in Escherichia coli by exploiting a curcuminoid synthase from Oryza sativa and a beta-oxidation pathway from Saccharomyces cerevisiae. Chembiochem, 11, 2034-2041.
  54. Akio Noguchi, Takeshi Kitamura, Hiroyasu Onaka, Sueharu Horinouchi, Yasuo Ohnishi. (2010) A copper-containing oxidase catalyzes C-nitrosation in nitrosobenzamide biosynthesis. Nat. Chem. Biol., 6, 641-643.
  55. Yohei Katsuyama, Yutaka Hirose, Nobutaka Funa, Yasuo Ohnishi, Sueharu Horinouchi. (2010) Precursor-directed biosynthesis of curcumin analogs in Escherichia coli. Biosci. Biotechnol. Biochem., 74, 641-645.
  56. Genki Akanuma, Hirofumi Hara, Yasuo Ohnishi, Sueharu Horinouchi. (2009) Dynamic changes in the extracellular proteome caused by absence of a pleiotropic regulator AdpA in Streptomyces griseus. Mol. Microbiol., 73, 898-912.
  57. Kazuya Marushima, Yasuo Ohnishi, Sueharu Horinouchi. (2009) CebR as a master regulator for cellulose/cellooligosaccharide catabolism affects morphological development in Streptomyces griseus. J. Bacteriol., 191, 5930-5940.
  58. Aya Iida, Yasuo Ohnishi, Sueharu Horinouchi. (2009) Identification and characterisation of target genes of the GinI/GinR quorum-sensing system in Gluconacetobacter intermedius. Microbiology, 155, 3021-3032.
  59. Takeaki Tezuka, Hirofumi Hara, Yasuo Ohnishi, Sueharu Horinouchi. (2009) Identification and gene disruption of small non-coding RNAs in Streptomyces griseus. J. Bacteriol., 191, 4896-4904.
  60. Hirofumi Hara, Yasuo Ohnishi, Sueharu Horinouchi. (2009) DNA microarray analysis of global gene regulation by A-factor in Streptomyces griseus. Microbiology, 155, 2197-2210.
  61. Hirokazu Suzuki, Kazuya Marushima, Yasuo Ohnishi, Sueharu Horinouchi. (2008) A novel pair of terminal protein and telomere-associated protein for replication of the linear chromosome of Streptomyces griseus IFO13350. Biosci. Biotechnol. Biochem., 72, 2973-2980.
  62. Aya Iida, Yasuo Ohnishi, Sueharu Horinouchi. (2008) An OmpA family protein, a target of the GinI/GinR quorum-sensing system in Gluconacetobacter intermedius controls acetic acid fermentation. J. Bacteriol., 190, 5009-5019.
  63. Yasuo Ohnishi, Jun Ishikawa, Hirofumi Hara, Hirokazu Suzuki, Miwa Ikenoya, Haruo Ikeda, Atsushi Yamashita, Masahira Hattori, Sueharu Horinouchi. (2008) The genome sequence of the streptomycin-producing microorganism Streptomyces griseus IFO 13350. J. Bacteriol., 190, 4050-4060.
  64. Setsu Hirano, Katsuyuki Tanaka, Yasuo Ohnishi, Sueharu Horinouchi. (2008) Conditionally positive effect of the TetR-family transcriptional regulator AtrA on streptomycin production by Streptomyces griseus. Microbiology, 154, 905-914.
  65. Aya Iida, Yasuo Ohnishi, Sueharu Horinouchi. (2008) Control of acetic acid fermentation by quorum sensing via N-acylhomoserine lactones in Gluconacetobacter intermedius. J. Bacteriol., 190, 2546-2555.
  66. Shigeo Suzuki, Eiyu Nakanishi, Keiko Furihata, Katsushiro Miyamoto, Hiroshi Tsujibo, Takeshi Watanabe, Yasuo Ohnishi, Sueharu Horinouchi, Hiromichi Nagasawa, Shohei Sakuda. (2008) Chitinase inhibitor allosamidin promotes chitinase production of Streptomyces generally. Int. J. Biol. Macromol., 43, 13-19.
  67. Hiromi Nishida, Yasuo Ohnishi, Teruhiko Beppu, Sueharu Horinouchi. (2007) Evolution of gamma-butyrolactone synthases and receptors in Streptomyces. Environ. Microbiol., 9, 1986-1994.
  68. Hirokazu Suzuki, Yasuo Ohnishi, Sueharu Horinouchi. (2007) GriC and GriD constitute a carboxylic acid reductase involved in grixazone biosynthesis in Streptomyces griseus. J. Antibiot., 60, 380-387.
  69. Akiko Tanaka, Yuji Takano, Yasuo Ohnishi, Sueharu Horinouchi. (2007) AfsR recruits RNA polymerase to the afsS promoter: a model for transcriptional activation by SARPs. J. Mol. Biol., 369, 322-333.
  70. Tatsuichiro Higashi, Yuko Iwasaki, Yasuo Ohnishi, Sueharu Horinouchi. (2007) A-factor and phosphate-depletion signals are transmitted to the grixazone biosynthesis genes via the pathway-specific transcriptional activator GriR. J. Bacteriol., 189, 3515-3524.
  71. Jun-ya Kato, Nobutaka Funa, Hidenori Watanabe, Yasuo Ohnishi, Sueharu Horinouchi. (2007) Biosynthesis of gamma-butyrolactone autoregulators that switch on secondary metabolism and morphological development in Streptomyces. Proc. Natl. Acad. Sci. USA., 104, 2378-2383.
  72. Hirokazu Suzuki, Yasuo Ohnishi, Sueharu Horinouchi. (2007) Arylamine N-acetyltransferase responsible for acetylation of 2-aminophenols in Streptomyces griseus. J. Bacteriol., 189, 2155-2159.
  73. Shigeo Suzuki, Eiyu Nakanishi, Tsuyoshi Ohira, Ryu Kawachi, Yasuo Ohnishi, Sueharu Horinouchi, Hiromichi Nagasawa, Shohei Sakuda. (2006) Chitinase inhibitor allosamidin is a signal molecule for chitinase production in its producing Streptomyces II. Mechanism for regulation of chitinase production by allosamidin through a two-component regulatory system. J. Antibiot., 59, 410-417.
  74. Hirokazu Suzuki, Yasuo Ohnishi, Yasuhide Furusho, Shohei Sakuda, Sueharu Horinouchi. (2006) Novel benzene ring biosynthesis from C3 and C4 primary metabolites by two enzymes. J. Biol. Chem., 281, 36944-36951.
  75. Setsu Hirano, Jun-ya Kato, Yasuo Ohnishi, Sueharu Horinouchi. (2006) Control of the Streptomyces Subtilisin inhibitor gene by AdpA in the A-factor regulatory cascade in Streptomyces griseus. J. Bacteriol., 188, 6207-6216.
  76. Ayami Tomono, Mari Mashiko, Tadahiro Shimazu, Hirotaka Inoue, Hiromichi Nagasawa, Minoru Yoshida, Yasuo Ohnishi, Sueharu Horinouchi. (2006) Self-activation of serine/threonine kinase AfsK on autophosphorylation at threonine-168. J. Antibiot., 59, 117-123.
  77. Hirokazu Suzuki, Yasuhide Furusho, Tatsuichiro Higashi, Yasuo Ohnishi, Sueharu Horinouchi. (2006) A novel o-aminophenol oxidase responsible for formation of the phenoxazinone chromophore of grixazone. J. Biol. Chem., 281, 824-833.
  78. Ikuo Miyahisa, Nobutaka Funa, Yasuo Ohnishi, Stefan Martens, Takaya Moriguchi, Sueharu Horinouchi. (2006) Combinatorial biosynthesis of flavones and flavonols in Escherichia coli. Appl. Microbiol. Biotechnol., 71, 53-58.
  79. Nobutaka Funa, Masanori Funabashi, Yasuo Ohnishi, Sueharu Horinouchi. (2005) Biosynthesis of hexahydroxyperylenequinone melanin via oxidative aryl coupling by cytochrome P-450 in Streptomyces griseus. J. Bacteriol., 187, 8149-8155.
  80. Ayami Tomono, Yisan Tsai, Yasuo Ohnishi, Sueharu Horinouchi. (2005) Three chymotrypsin genes are members of the AdpA regulon in the A-factor regulatory cascade in Streptomyces griseus. J. Bacteriol., 187, 6341-6353.
  81. Jun-ya Kato, Setsu Hirano, Yasuo Ohnishi, Sueharu Horinouchi. (2005) The Streptomyces subtilisin inhibitor (SSI) gene in Streptomyces coelicolor A3(2). Biosci. Biotechnol. Biochem., 69, 1624-1629.
  82. Ayami Tomono, Yisan Tsai, Haruka Yamazaki, Yasuo Ohnishi, Sueharu Horinouchi. (2005) Transcriptional control by A-factor of strR, the pathway-specific transcriptional activator for streptomycin biosynthesis in Streptomyces griseus. J. Bacteriol., 187, 5595-5604.
  83. Jun-ya Kato, Yasuo Ohnishi, Sueharu Horinouchi. (2005) Autorepression of AdpA of the AraC/XylS family, a key transcriptional activator in the A-factor regulatory cascade in Streptomyces griseus. J. Mol. Biol., 350, 12-26.
  84. Ikuo Miyahisa, Masafumi Kaneko, Nobutaka Funa, Hisashi Kawasaki, Hiroyuki Kojima, Yasuo Ohnishi, Sueharu Horinouchi. (2005) Efficient production of (2S)-flavanones by Escherichia coli containing an artificial biosynthetic gene cluster. Appl. Microbiol. Biotechnol., 68, 498-504.
  85. Tohru Yonekawa, Yasuo Ohnishi, Sueharu Horinouchi. (2005) A calmodulin-like protein in the bacterial genus Streptomyces. FEMS Microbiol. Lett., 244, 315-321.
  86. Jun-ya Kato, Won-Jae Chi, Yasuo Ohnishi, Soon-Kwang Hong, Sueharu Horinouchi. (2005) Transcriptional control by A-factor of two trypsin genes in Streptomyces griseus. J. Bacteriol., 187, 286-295.
  87. Haruka Yamazaki, Ayami Tomono, Yasuo Ohnishi, Sueharu Horinouchi. (2004) DNA-binding specificity of AdpA, a transcriptional activator in the A-factor regulatory cascade in Streptomyces griseus. Mol. Microbiol., 53, 555-572.
  88. Yasuo Ohnishi, Yasuhide Furusho, Tatsuichiro Higashi, Hyo-Kon Chun, Kazuo Furihata, Shohei Sakuda, Sueharu Horinouchi. (2004) Structures of grixazone A and B, A-factor-dependent yellow pigments produced under phosphate depletion by Streptomyces griseus. J. Antibiot., 57, 218-223.
  89. Jun-ya Kato, Ikuo Miyahisa, Mari Mashiko, Yasuo Ohnishi, Sueharu Horinouchi. (2004) A single target is sufficient to account for the biological effects of the A-factor receptor protein of Streptomyces griseus. J. Bacteriol., 186, 2206-2211.
  90. Ryo Natsume, Yasuo Ohnishi, Toshiya Senda, Sueharu Horinouchi. (2004) Crystal structure of a gamma-butyrolactone autoregulator receptor protein in Streptomyces coelicolor A3(2). J. Mol. Biol., 336, 409-419.
  91. Ryo Natsume, Ryo Takeshita, Masakazu Sugiyama, Yasuo Ohnishi, Toshiya Senda, Sueharu Horinouchi. (2003) Crystallization of CprB, an autoregulator-receptor protein from Streptomyces coelicolor A3(2). Acta Crystallogr. D Biol. Crystallogr., 59, 2313-2315.
  92. Haruka Yamazaki, Yuji Takano, Yasuo Ohnishi, Sueharu Horinouchi. (2003) amfR, an essential gene for aerial mycelium formation, is a member of the AdpA regulon in the A-factor regulatory cascade in Streptomyces griseus. Mol. Microbiol., 50, 1173-1187
  93. Eui Il Hwang, Masafumi Kaneko, Yasuo Ohnishi, Sueharu Horinouchi. (2003) Production of plant-specific flavanones by Escherichia coli containing an artificial gene cluster. Appl. Environ. Microbiol., 69, 2699-2706.
  94. Haruka Yamazaki, Yasuo Ohnishi, Sueharu Horinouchi. (2003) Transcriptional switch on of ssgA by A-factor, which is essential for spore septum formation in Streptomyces griseus. J. Bacteriol., 185, 1273-1283.
  95. Masafumi Kaneko, Yasuo Ohnishi, Sueharu Horinouchi. (2003) Cinnamate:coenzyme A ligase from the filamentous bacterium Streptomyces coelicolor A3(2). J. Bacteriol., 185, 20-27
  96. Hyo-Kon Chun, Yasuo Ohnishi, Kazutoshi Shindo, Norihiko Misawa, Kensuke Furukawa, Sueharu Horinouchi. (2003) Biotransformation of flavone and flavanone by Streptomyces lividans cells carrying shuffled biphenyl dioxygenase genes. J. Mol. Catal. B-Enzym., 21, 113-121.
  97. Kazutoshi Shindo, Ryoko Nakamura, Ikuko Chinda, Yasuo Ohnishi, Sueharu Horinouchi, Haruko Takahashi, Kazuo Iguchi, Shigeaki Harayama, Kensuke Furukawa, Norihiko Misawa. (2003) Hydroxylation of ionized aromatics including carboxylic acid or amine using recombinant Streptomyces lividans cells expressing modified biphenyl dioxygenase genes. Tetrahedron, 59, 1895-1900.
  98. Jun-ya Kato, Ayano Suzuki, Haruka Yamazaki, Yasuo Ohnishi, Sueharu Horinouchi. (2002) Control by A-factor of a metalloendopeptidase gene involved in aerial mycelium formation in Streptomyces griseus. J. Bacteriol., 184, 6016-6025.
  99. Nobutaka Funa, Yasuo Ohnishi, Yutaka Ebizuka, Sueharu Horinouchi. (2002) Alterations of reaction and substrate specificity of bacterial type III polyketide synthase by site-directed mutagenesis. Biochem. J., 367, 781-789.
  100. Kenji Ueda, Ken-ichi Oinuma, Go Ikeda, Kuniaki Hosono, Yasuo Ohnishi, Sueharu Horinouchi, Teruhiko Beppu. (2002) AmfS, an extracellular peptidic morphogen in Streptomyces griseus. J. Bacteriol., 184, 1488-1492.
  101. Jeong-Woo Seo, Yasuo Ohnishi, Aiko Hirata, Sueharu Horinouchi. (2002) ATP-binding cassette transport system involved in regulation of morphological differentiation in response to glucose in Streptomyces griseus. J. Bacteriol., 184, 91-103.
  102. Nobutaka Funa, Yasuo Ohnishi, Yutaka Ebizuka, Sueharu Horinouchi. (2002) Properties and substrate specificity of RppA, a chalcone synthase-related polyketide synthase in Streptomyces griseus. J. Biol. Chem., 277, 4628-4635.
  103. Kazutoshi Shindo, Yasuo Ohnishi, Hyo-Kon Chun, Haruko Takahashi, Miki Hayashi, Atsushi Saito, Kazuo Iguchi, Kensuke Furukawa, Shigeaki Harayama, Sueharu Horinouchi, Norihiko Misawa. (2001) Oxygenation reactions of various tricyclic fused aromatic compounds using Escherichia coli and Streptomyces lividans transformants carrying several arene dioxygenase genes. Biosci. Biotechnol. Biochem., 65, 2472-2481.
  104. Hyo-Kon Chun, Yasuo Ohnishi, Norihiko Misawa, Kazutoshi Shindo, Miki Hayashi, Shigeaki Harayama, Sueharu Horinouchi. (2001) Biotransformation of phenanthrene and 1-methoxynaphthalene with Streptomyces lividans cells expressing a marine bacterial phenanthrene dioxygenase gene cluster. Biosci. Biotechnol. Biochem., 65, 1774-1781.
  105. Tohru Yonekawa, Yasuo Ohnishi, Sueharu Horinouchi. (2001) A calcium-binding protein with four EF-hand motifs in Streptomyces ambofaciens. Biosci. Biotechnol. Biochem., 65, 156-160.
  106. Han-Seung Lee, Yasuo Ohnishi, Sueharu Horinouchi. (2001) A sigma-B-like factor responsible for carotenoid biosynthesis in Streptomyces griseus. J. Mol. Microbiol. Biotechnol., 3, 95-101.
  107. Yasuo Ohnishi, Yoko Nishiyama, Rie Sato, Shogo Kameyama, Sueharu Horinouchi. (2000) An oligoribonuclease gene in Streptomyces griseus. J. Bacteriol., 182, 4647-4653.
  108. Haruka Yamazaki, Yasuo Ohnishi, Sueharu Horinouchi. (2000) An A-factor-dependent extracytoplasmic function sigma factor (sigmaAdsA) that is essential for morphological development in Streptomyces griseus. J. Bacteriol., 182, 4596-4605.
  109. Sueharu Horinouchi, Hiroyasu Onaka, Haruka Yamazaki, Shogo Kameyama, Yasuo Ohnishi. (2000) Isolation of DNA fragments bound by transcriptional factors, AdpA and ArpA, in the A-factor regulatory cascade. Actinomycetologica, 14, 37-42.
  110. Yasuo Ohnishi, Shogo Kameyama, Hiroyasu Onaka, Sueharu Horinouchi. (1999) The A-factor regulatory cascade leading to streptomycin biosynthesis in Streptomyces griseus: identification of a target gene of the A-factor receptor. Mol. Microbiol., 34, 102-111.
  111. Kyoko Takeuchi, Haruka Yamazaki, Norihiko Shiraishi, Yasuo Ohnishi, Yoshihisa Nishikawa, Sueharu Horinouchi. (1999) Characterization of an alg2 mutant of the zygomycete fungus Rhizomucor pusillus. Glycobiology, 9, 1287-1293.
  112. Haruka Yamazaki, Yasuo Ohnishi, Kyoko Takeuchi, Nobuhiko Mori, Norihiko Shiraishi, Yoshinori Sakata, Hirofumi Suzuki, Sueharu Horinouchi. (1999) Genetic transformation of a Rhizomucor pusillus mutant defective in asparagine-linked glycosylation: production of a milk-clotting enzyme in a less-glycosylated form. Appl. Microbiol. Biotechnol., 52, 401-409.
  113. Tohru Yonekawa, Yasuo Ohnishi, Sueharu Horinouchi. (1999) Involvement of amfC in physiological and morphological development in Streptomyces coelicolor A3(2). Microbiology, 145, 2273-2280.
  114. Nobutaka Funa, Yasuo Ohnishi, Isao Fujii, Masaaki Shibuya, Yutaka Ebizuka, Sueharu Horinouchi. (1999) A new pathway for polyketide synthesis in microorganisms. Nature, 400, 897-899.
  115. Haruka Yamazaki, Norihiko Shiraishi, Kyoko Takeuchi, Yasuo Ohnishi, Sueharu Horinouchi. (1998) Characterization of alg2 encoding a mannosyltransferase in the zygomycete fungus Rhizomucor pusillus. Gene, 221, 179-184.
  116. Koki Saito, Haruka Yamazaki, Yasuo Ohnishi, Satoshi Fujimoto, Eisaku Takahashi, Sueharu Horinouchi. (1998) Production of trehalose synthase from a basidiomycete, Grifola frondosa, in Escherichia coli. Appl. Microbiol. Biotechnol., 50, 193-198.
  117. Yasuo Ohnishi, Teruhiko Beppu, Sueharu Horinouchi. (1997) Two genes encoding serine protease homologues in Serratia marcescens and characterization of their products in Escherichia coli. J. Biochem., 121, 902-913.
  118. Yasuo Ohnishi, Makoto Nishiyama, Sueharu Horinouchi, Teruhiko Beppu. (1994) Involvement of the COOH-terminal pro-sequence of Serratia marcescens serine protease in the folding of the mature enzyme. J. Biol. Chem., 269, 32800-32806.
  119. Kohei Shimada, Yasuo Ohnishi, Sueharu Horinouchi, Teruhiko Beppu. (1994) Extracellular transport of pseudoazurin of Alcaligenes faecalis in Escherichia coli using the COOH-terminal domain of Serratia marcescens serine protease. J. Biochem., 116, 327-334.
  120. Shitsuw Shikata, Kohei Shimada, Yasuo Ohnishi, Sueharu Horinouchi, Teruhiko Beppu. (1993) Characterization of secretory intermediates of Serratia marcescens serine protease produced during its extracellular secretion from Escherichia coli cells. J. Biochem., 114, 723-731.
  121. Clyde A. Kelly, Makoto Nishiyama, Yasuo Ohnishi, Teruhiko Beppu, Jens J. Birktoft. (1993) Determinants of protein thermostability observed in the 1.9-Å crystal structure of malate dehydrogenase from the thermophilic bacterium Thermus flavus. Biochemistry, 32, 3913-3922.

【総説】

英文

  1. Takeaki Tezuka, Yasuo Ohnishi. (2014) Chapter 16: Microbial Hormones as a Master Switch for Secondary Metabolism in Streptomyces. Microbial Production From Genome Design to Cell Engineering, Springer, pp. 179-190.
  2. Yohei Katsuyama, Yasuo Ohnishi. (2012) Type III Polyketide Synthases in Microorganisms. Methods Enzymol. 515, 359-377.
  3. Yasuo Ohnishi, Haruka Yamazaki, Jun-ya Kato, Ayami Tomono, Sueharu Horinouchi. (2005) AdpA, a central transcriptional regulator in the A-factor regulatory cascade that leads to morphological development and secondary metabolism in Streptomyces griseus. Biosci. Biotechnol. Biochem., 69, 431-439.
  4. Yasuo Ohnishi, Sueharu Horinouchi. (2004) The A-factor regulatory cascade that leads to morphological development and secondary metabolism in Streptomyces. Biofilms, 1, 319-328.
  5. Yasuo Ohnishi. (2003) The A-factor regulatory cascade in the regulation of physiological and morphological development in Streptomyces griseus. Actinomycetologica, 17, 23-27.
  6. Masafumi Kaneko, Eui Il Hwang, Yasuo Ohnishi, Sueharu Horinouchi. (2003) Heterologous production of flavanones in Escherichia coli: potential for combinatorial biosynthesis of flavonoids in bacteria. J. Ind. Microbiol. Biotechnol., 30, 456-461.
  7. Yasuo Ohnishi, Jeong-Woo Seo, Sueharu Horinouchi. (2002) Deprogrammed sporulation in Streptomyces. FEMS Microbiol. Lett., 216, 1-7.
  8. Sueharu Horinouchi, Yasuo Ohnishi, Dae-Kyung Kang. (2001) The A-factor regulatory cascade and cAMP in the regulation of physiological and morphological development in Streptomyces griseus. J. Ind. Microbiol. Biotechnol., 27, 177-182.
  9. Yasuo Ohnishi, Sueharu Horinouchi. (1996) Extracellular production of a Serratia marcescens serine protease in Escherichia coli. Biosci. Biotechnol. Biochem., 60, 1551-1558.

和文

  1. 勝山 陽平, 大西 康夫 (2017) ジアゾ基を含む天然物の生合成に関与する新規亜硝酸生合成経路の発見〜二次代謝産物生合成に特異的な亜硝酸生合成経路〜 化学と生物、55巻、306-307
  2. 勝山陽平、菅井佳宜、大西康夫 (2017) 放線菌においてジアゾ基の生合成を担う亜硝酸生合成経路 バイオサイエンスとインダストリー、75巻、46-47
  3. 原啓文、大西康夫、福田雅夫 
(2011)
    ゲノム情報を基盤にした放線菌群の転写制御機構研究
    化学と生物 49, 234-242.
  4. 宮川拓也、大西康夫、田之倉優、堀之内末治 (2009)
    放線菌における有用物質生産制御因子の構造・機能解析
    蛋白質核酸酵素 54, 1696-1701.
  5. 大西康夫、堀之内末治 (2009)
    微生物ホルモンのケミカルバイオロジー
    化学と生物 47, 419-426.
  6. 大西康夫、原啓文、堀之内末治 (2009)
    微生物ホルモンのケミカルジェネティクス
    細胞工学 28, 360-365.
  7. 大西康夫、堀之内末治 (2008)
    新規なベンゼン環合成経路-有用物質生産への応用
    バイオサイエンスとインダストリー 66, 25-27.
  8. 大西康夫 (2003)
    放線菌の二次代謝・形態分化に関する分子遺伝学的研究
    日本農芸化学会誌 77, 852-859.
  9. 大西康夫、堀之内末治 (2002)
    A-ファクターによる放線菌の二次代謝・形態分化の開始機構
    化学と生物 40, 185-190.
  10. 大西康夫、堀之内末治 (1999)
    微生物ホルモンによる放線菌の2次代謝・形態分化開始の制御機構
    蛋白質核酸酵素 44, 1552-1561.
  11. 大西康夫、山崎美佳、堀之内末治 (1999)
    毛かびの糖鎖付加変異株 -蛋白質分泌生産への応用-
    バイオサイエンスとインダストリー 57, 804-809.
  12. 大西康夫、堀之内末治 (1998)
    セラチアセリンプロテアーゼ分泌機構の解明と利用 
    バイオサイエンスとインダストリー 56, 679-682.

【著書】

  1. Takeaki Tezuka, Yasuo Ohnishi (2014)
    Chapter 16: Microbial Hormones as a Master Switch for Secondary Metabolism in Streptomyces
    Microbial Production From Genome Design to Cell Engineering (Editors: Hideharu Anazawa and Sakayu Shimizu), Springer, pp. 179-190.
  2. 手塚武揚、大西康夫 (2012)
    放線菌の二次代謝産物生産を誘導する微生物ホルモン
    微生物を活用した新世代の有用物質生産技術(穴澤秀治 監修)CMC出版 pp. 269-280.
  3. 大西康夫、堀之内末治 (2003)
    微生物を生産工場とした有用物質生産 -新しい微生物利用技術-
    微生物の取扱いと利用・応用技術 情報機構 pp. 213-223.
  4. 大西康夫、堀之内末治 (1995)
    セラチア由来のセリンプロテアーゼの大腸菌における菌体外分泌機構
    微生物機能の多様性(別府輝彦 編) 学会出版センター pp. 269-280.

【国際学会・国際シンポジウムでの講演】

  1. “Coupled binuclear copper enzymes involved in the secondary metabolite biosynthesis in Streptomyces” Enzyme Engineering XXII: Emerging Topics in Enzyme Engineering (An ECI Conference Series), Toyama (Sep. 25, 2013)
  2. “Regulation of grixazone biosynthesis in Streptomyces griseus” First China-Europe Symposium “The Biology of Actinomycete Antibiotic Production”, Beijing, China (Apr. 10, 2013)
  3. “Biosynthetic pathway for ferroverdin A, an inhibitor of cholesteryl ester transfer protein, in Streptomyces sp. WK-5344” 13th Swiss-Japanese Conference on Biotechnology and Bioprocess Engineering, Walzenhausen, Switzerland, (Nov. 6, 2012)
  4. “An alternative sigma factor governs the principal sigma factor throughout growth in Streptomyces griseus” 2012 International Symposium of the Korean Society for Microbiology and Biotechnology, Busan, Korea, (Jun. 27, 2012)
  5. “Biosynthesis of ferroverdin in Streptomyces sp. WK-5344” The International Conference of Natural Product Biosynthesis (8th US-Japan Seminar), Awaji, (Jun. 21, 2012)
  6. “Novel regulatory circuits for the morphological and physiological differentiation in Streptomyces griseus” 16th International Symposium on the Biology of Actinomycetes, Puerto Vallarta, Mexico (Dec. 12, 2011)
  7. “Novel nitroso-forming o-aminophenol oxidase involved in 4-hydroxy-3-nitrosobenzamide biosynthesis” International Chemical Congress of Pacific Basin Societies, Honolulu, Hawaii (Dec. 17, 2010)
  8. “Genome-wide analysis of the AdpA regulon in Streptomyces griseus” 2012 International Symposium of the Korean Society for Microbiology and Biotechnology, Seoul, Korea (Jun. 25, 2010)
  9. “Regulation of secondary metabolism and morphogenesis by A-factor in Streptomyces griseus” International Symposium in the JSBBA meeting, Tokyo (Mar. 30, 2010)
  10. “The A-factor regulatory cascade in Streptomyces griseus” (Plenary Lecture) 15th International Symposium on the Biology of Actinomycetes, Shanghai, China (Aug. 23, 2009)
  11. “Genome-wide analysis of the A-factor regulatory cascade in Streptomyces griseus” International Symposium (Japanese Society for Bacteriology): Bacterial Genome Engineering, Nagoya (Mar. 3, 2009)
  12. “Regulation of gene expression by a microbial hormone in Streptomyces” International Symposium: Bacteria Made Organelles Made Eukaryotic Cells, Tokyo (Nov. 29, 2008)
  13. “Genome-wide analysis of the A-factor regulatory cascade” UK-Japan Workshop 2008: Genomics of Antibiotic-producing Actinomycetes: Implications and Applications, Tokyo (Oct. 31, 2008)
  14. “Transcriptome and proteome analysis of the A-factor regulatory cascade in Streptomyces griseus” 4th Japan-Finland Biotechnology Symposium, Sapporo (Oct. 2, 2008)
  15. “Complete genome sequence of Streptomyces griseus” 14th International Symposium on the Biology of Actinomycetes, Newcastle, England (Aug. 27, 2007)
  16. “Regulation of grixazone biosynthesis by Streptomyces griseus” 10th International Symposium on the Genetics of Industrial Microorganisms, Prague, Czech Republic (Jun. 26, 2006)
  17. “The biosynthetic pathway for grixazone” 3rd Japan-Finland Biotechnology Symposium, Parainen, Finland (Aug. 9, 2006)
  18. “Cloning and characterization of the grixazone biosynthetic gene cluster” and “AfsR recruits RNA polymerase on the afsS promoter - a model of transcriptional activation by SARPs” Workshop on the Present and Future of Actinomycete Research, Yongin, Korea (Sep. 22, 2006)
  19. “Biosynthesis of an A-factor-dependent yellow pigment (grixazone) in Streptomyces griseus” International Chemical Congress of Pacific Basin Societies, Honolulu, Hawaii (Dec. 18, 2005)
  20. “The AdpA regulon in the A-factor regulatory cascade that leads to morphological differentiation in Streptomyces griseus” 13th International Symposium on the Biology of Actinomycetes, Melbourne, Australia (Dec. 2, 2003)
  21. “Regulation of secondary metabolism and morphological differentiation by A-factor in Streptomyces griseus” 9th International Symposium on the Genetics of Industrial Microorganisms, Gyeongju, Korea (Jul. 5, 2002)