CRISPR/Cpf1-mediated mutagenesis and gene deletion in industrial filamentous fungi Aspergillus oryzae and Aspergillus sojae
T. Katayama, J. Maruyama
J. Biosci. Bioeng., 133, 353–361 (2022)
Downregulation of the ypdA gene encoding an intermediate of His-Asp phosphorelay signaling in Aspergillus nidulans induces the same cellular effects as the phenylpyrrole fungicide fludioxonil
A. Yoshimi, D. Hagiwara, M. Ono, Y. Fukuma, Y. Midorikawa, K. Furukawa, T. Fujioka, O. Mizutani, N. Sato, K. Miyazawa, J. Maruyama, J. Marui, Y. Yamagata, T. Nakajima, C. Tanaka, K. Abe
Front. Fungal Biol., in press (2021)
Novel Fus3- and Ste12-interacting protein FsiA activates cell fusion-related genes in both Ste12-dependent and -independent manners in Ascomycete filamentous fungi
T. Katayama, Ö. Bayram, T. Mo, B. Karahoda, O. Valerius, D. Takemoto, G. H. Braus, K. Kitamoto, J. Maruyama
Mol. Microbiol., 115, 723–738 (2021)
Discovery of the 2,4'-dihydroxy-3'-methoxypropiophenone biosynthesis genes in Aspergillus oryzae
E. Kan, H. Tomita, Y. Katsuyama, J. Maruyama, Y. Koyama, Y. Ohnishi
Chembiochem, 22, 203–211 (2020)
Biosynthesis of indole diterpene lolitrems: Radical-induced cyclization of an epoxyalcohol affording a characteristic lolitremane skeleton
Y. Jiang, T. Ozaki, M. Harada, T. Miyasaka, H. Sato, K. Miyamoto, J. Kanazawa, C. Liu, J. Maruyama, M. Adachi, A. Nakazaki, T. Nishikawa, M. Uchiyama, A. Minami, H. Oikawa
Angew. Chem. Int. Ed., 59, 17996–18002 (2020)
A CRISPR/Cas9-mediated gene knockout system in Aspergillus luchuensis mut. kawachii
C. Kadooka, M. Yamaguchi, K. Okutsu, Y. Yoshizaki, K. Takamine, T. Katayama, J. Maruyama, H. Tamaki, T. Futagami
Biosci. Biotechnol. Biochem., 84, 2179–2183 (2020)
A nuclear protein NsiA from Epichloë festucae interacts with a MAP kinase MpkB and regulates the expression of genes required for symbiotic infection and hyphal cell fusion.
A. Tanaka, S. Kamiya, Y. Ozaki, Y. Kayano, S. Kameoka, S. Sanjay, F. Akano, A. Uemura, H. Takagi, R. Terauchi, J. Maruyama, A. Fleissner, B. Scott, D. Takemoto
Mol. Microbiol., 114, 626–640 (2020)
Invasive growth of Aspergillus oryzae in rice koji and increase of nuclear number
M. Yasui, K. Oda, S. Masuo, S. Hosoda, T. Katayama, J. Maruyama, N. Takaya, N. Takeshita
Fungal Biol. Biotechnol., 7, 8 (2020)
Functional production of human antibody by the filamentous fungus Aspergillus oryzae
H.H. Huynh, N. Morita, T. Sakamoto, T. Katayama, T. Miyakawa, M. Tanokura, Y. Chiba, R. Shinkura, J. Maruyama
Fungal Biol. Biotechnol., 7, 7 (2020)
A novel Pezizomycotina-specific protein with gelsolin domains regulates contractile actin ring assembly and constriction in perforated septum formation
M.A.A. Mamun, T. Katayama, W. Cao, S. Nakamura, J. Maruyama
Mol. Microbiol., 113, 964–982 (2020)
Efficient heterologous production of atrochrysone carboxylic acid-related polyketides in an Aspergillus oryzae host with enhanced malonyl-coenzyme A supply
E. Kan, Y. Katsuyama, J. Maruyama, K. Tamano, Y. Koyama, Y. Ohnishi
J. Gen. Appl. Microbiol., 66, 195–199 (2019)
Efficient reconstitution of Basidiomycota diterpene erinacine gene cluster in Ascomycota host Aspergillus oryzae based on genomic DNA sequences
C. Liu, A. Minami, T. Ozaki, J. Wu, H. Kawagishi, J. Maruyama, H. Oikawa
J. Am. Chem. Soc. Vol. 141, 15519–15523 (2019)
Genome editing to generate nonfoam-forming sake yeast strains
S. Ohnuki, M. Kashima, T. Yamada, F. Ghanegolmohammadi, Y. Zhou, T. Goshima, J. Maruyama, K. Kitamoto, D. Hirata, T. Akao, Y. Ohya
Biosci. Biotechnol. Biochem., 83, 1583–1593 (2019)
Inter-strain expression of sequence-diverse HET domain genes severely inhibits growth of Aspergillus oryzae
N. Mori, T. Katayama, R. Saito, K. Iwashita, J. Maruyama
Biosci. Biotechnol. Biochem., 83, 1557–1569 (2019)
Ascomycota Aspergillus oryzae is an efficient expression host for production of Basidiomycota terpenes using genomic DNA sequences.
S. Nagamine, C. Liu, J. Nishishita, T. Kozaki, K. Sogahata, Y. Sato, A. Minami, T. Ozaki, C. Schmidt-Dannert, J. Maruyama, H. Oikawa
Appl. Environ. Microbiol., 85, e00409-19 (2019)
Production of the plant polyketide curcumin in Aspergillus oryzae: strengthening malonyl-CoA supply for yield improvement
E. Kan, Y. Katsuyama, J. Maruyama, K. Tamano, Y. Koyama, Y. Ohnishi
Biosci. Biotechnol. Biochem., 83, 1372–1381 (2019)
富山県産大麦麦芽から分離した酵母菌株「とやま産まれの酵母」の清酒醸造特性
尾仲 宏康, 丸山 潤一, 浅水 俊平, 黒岩 真弓, 北本 勝ひこ, 山田 雅人, 五島 徹也, 赤尾 健
日本醸造協会誌, 114, 645–653 (2019)
Forced recycling of an AMA1-based genome-editing plasmid allows for efficient multiple gene deletion/integration in the industrial filamentous fungus Aspergillus oryzae
T. Katayama, H. Nakamura, Y. Zhang, A. Pascal, W. Fujii, J. Maruyama J
Appl. Environ. Microbiol., 85, e01896-18 (2019)
BiFC-based visualisation system reveals cell fusion morphology and heterokaryon incompatibility in the filamentous fungus Aspergillus oryzae
Okabe T. Okabe, T. Katayama, T. Mo, N. Mori, F. J. Jin, I. Fujii, K. Iwashita, K. Kitamoto, J. Maruyama
Sci. Rep., 8, 2922 (2018)
Highly efficient gene targeting in Aspergillus oryzaeindustrial strains under ligD mutation introduced by genome editing: Strain-specific differences in the effects of deleting EcdR, the negative regulator of sclerotia formation
H. Nakamura, T. Katayama, T. Okabe, K. Iwashita, W. Fujii, K. Kitamoto, J. Maruyama
J. Gen. Appl. Microbiol., 63,172-178 (2017)
AoAtg26, a putative sterol glucosyltransferase, is required for autophagic degradation of peroxisomes, mitochondria, and nuclei in the filamentous fungus Aspergillus oryzae
T. Kikuma, T. Tadokoro, J. Maruyama, K. Kitamoto
Biosci. Biotechnol Biochem., 81, 384-395 (2017)
Carbon and nitrogen depletion-induced nucleophagy and selective autophagic sequestration of a whole nucleus in multinucleate cells of the filamentous fungus Aspergillus oryzae
T. Kikuma, T. Mitani, T. Kohara, J. Maruyama, K. Kitamoto
J. Gen. Appl. Microbiol., 63, 139-146 (2017)
Comparative genomic analysis identified a mutation related to enhanced heterologous protein production in the filamentous fungus Aspergillus oryzae
F. J. Jin, T. Katayama, J. Maruyama, K. Kitamoto
Appl. Microbiol. Biotechnol., 100, 9163-9174 (2016)
Molecular dissection of SO (SOFT) protein in stress-induced aggregation and cell-to-cell interactive functions in filamentous fungal multicellularity
W. Tsukasaki, K. Saeki, T. Katayama, J. Maruyama, K. Kitamoto
Fungal Biol., 120, 775-782 (2016)
Development of a genome editing technique using the CRISPR/Cas9 system in the industrial filamentous fungus Aspergillus oryzae
T. Katayama, Y. Tanaka, T. Okabe, H. Nakamura, W. Fujii, K. Kitamoto, J. Maruyama
Biotechnol. Lett., 38, 637-642 (2016)
AoRim15 is involved in conidial stress tolerance, conidiation and sclerotia formation in the filamentous fungus Aspergillus oryzae
H. Nakamura, T. Kikuma, F. J. Jin, J. Maruyama, K. Kitamoto
J. Biosci. Bioeng., 121, 365-371 (2016)
Modulating ER-Golgi cargo receptors for improving secretion of carrier-fused heterologous proteins in the filamentous fungus Aspergillus oryzae
H. Hoang, J. Maruyama, K. Kitamoto
Appl. Environ. Microbiol., 81,533-543 (2015)
A large non-conserved region of the tethering protein Leashin is involved in regulating the position, movement and function of Woronin bodies in Aspergillus oryzae
P. Han, F. J. Jin, J. Maruyama, K. Kitamoto
Eukaryot. Cell, 13, 866-877 (2014)
Establishment of a new method to quantitatively evaluate hyphal fusion ability in Aspergillus oryzae
W. Tsukasaki, J. Maruyama, K. Kitamoto
Biosci. Biotechnol. Biochem., 78, 1254-62 (2014)
Efficient formation of heterokaryotic sclerotia in the filamentous fungus Aspergillus oryzae
R. Wada, F. J. Jin, Y. Koyama, J. Maruyama, K. Kitamoto
Appl. Microbiol. Biotechnol., 98, 325-334 (2014)
Aspergillus oryzae AoSO Is a novel component of stress granules upon heat stress in filamentous fungi
H.-T. Huang, J. Maruyama, K. Kitamoto
PLoS ONE, 8, e72209 (2013)
Enhanced production of bovine chymosin by autophagy deficiency in the filamentous fungus Aspergillus oryzae
J. Yoon, T. Kikuma, J. Maruyama, K. Kitamoto
PLoS ONE, 8, e62512 (2013)
Further enhanced production of heterologous proteins by double-gene disruption (ΔAosedD ΔAovps10) in a hyper-producing mutant of Aspergillus oryzae
L. Zhu, J. Maruyama, K. Kitamoto
Appl. Microbiol. Biotechnol., 97, 6347-6357 (2013)
Hyphal heterogeneity in Aspergillus oryzae is the result of dynamic closure of septa by Woronin bodies
R. J. Bleichrodt, G. J. van Veluw, B. Recter, J. Maruyama, K. Kitamoto, H. A. Wösten
Mol Microbiol., 86, 1334-1344 (2012)
The Aspergillus nidulans MAPK Module AnSte11-Ste50-Ste7-Fus3 Controls Development and Secondary Metabolism
Ö. Bayram, Ö. S. Bayram, Y. L. Ahmed, J. Maruyama, O. Valerius, S. O. Rizzoli, R. Ficner, S. Irniger, G. H. Braus
PLoS Genet., 8, e1002816 (2012)
Contribution ratios of amyA, amyB, amyC genes to high-level α-amylase expression in Aspergillus oryzae
T. Nemoto, J. Maruyama, K. Kitamoto
Biosci. Biotechnol. Biochem., 76, 1477-1483 (2012)
Improved heterologous protein production by a tripeptidyl peptidase gene (AosedD) disruptant of the filamentous fungus Aspergillus oryzae
L. Zhu, T. Nemoto, J. Yoon, J. Maruyama, K. Kitamoto
J. Gen. Appl. Microbiol., 58, 199-209 (2012)
Presence and functionality of mating-type genes in the supposedly asexual filamentous fungus Aspergillus oryzae
R. Wada, J. Maruyama, H. Yamaguchi, N. Yamamoto, Y. Wagu, M. Paoletti, D. B. Archer, P. S. Dyer, K. Kitamoto
Appl. Environ. Microbiol., 78, 2819-2829 (2012)
Peroxisomes are involved in biotin biosynthesis in Aspergillus and Arabidopsis
Y. Tanabe, J. Maruyama, S. Yamaoka, D. Yahagi, I. Matsuo, N. Tsutsumi, K. Kitamoto
J. Biol. Chem., 286, 30455-30461 (2011)
A carrier fusion significantly induces unfolded protein response in heterologous protein production by Aspergillus oryzae
A. Ohno, J. Maruyama, T. Nemoto, M. Arioka, K. Kitamoto
Appl. Microbiol. Biotechnol., 92, 1197-1206 (2011)
SclR, a basic helix-loop-helix transcription factor, regulates hyphal morphology and promotes sclerotial formation in Aspergillus oryzae
F. J. Jin, T. Takahashi, K. Matsushima, S. Hara, Y. Shinohara, J. Maruyama, K. Kitamoto, Y. Koyama
Eukaryot. Cell, 10, 945-955 (2011)
Autophagy delivers misfolded secretory proteins accumulated in endoplasmic reticulum to vacuoles in the filamentous fungus Aspergillus oryzae
S. Kimura, J. Maruyama, T. Kikuma, M. Arioka, K. Kitamoto
Biochem. Biophys. Res. Commun., 406, 464-470 (2011)
Functional analysis and subcellular location of two flavohemoglobins from Aspergillus oryzae
S. Zhou, S. Fushinobu, S. W. Kim, Z. Zhou, Y. Nakanishi, J. Maruyama, K. Kitamoto, T. Wakagi, H. Shoun
Fungal Genet. Biol., 48, 200-207 (2011)
Disruption of ten protease genes in the filamentous fungus Aspergillus oryzae highly improves production of heterologous proteins
J. Yoon, J. Maruyama, K. Kitamoto
Appl. Microbiol. Biotechnol., 89, 747-759 (2011)
In vivo imaging of endoplasmic reticulum and distribution of mutant α-amylase in Aspergillus oryzae
S. Kimura, J. Maruyama, T. Watanabe, Y. Ito, M. Arioka, K. Kitamoto
Fungal Genet. Biol., 47, 1044-1054 (2010)
Enhanced production and secretion of heterologous proteins by the filamentous fungus Aspergillus oryzae via disruption of vacuolar protein sorting receptor gene Aovps10
J. Yoon, T. Aishan, J. Maruyama, K. Kitamoto
Appl. Environ. Microbiol., 76, 5718-5727 (2010)
A Eukaryotic Copper-Containing Nitrite Reductase Derived from a NirK Homolog Gene of Aspergillus oryzae
Y. Nakanishi, S. Zhou, S. W. Kim, S. Fushinobu, J. Maruyama, K. Kitamoto, T. Wakagi, H. Shoun
Biosci. Biotechnol. Biochem., 74, 984-991 (2010)
Promotion of efficient Saccharification with Aspergillus fumigatus AfSwo1 towards crystalline cellulose
X. Chen, N. Ishida, N. Todaka, R. Nakamura, J. Maruyama, H. Takahashi, K. Kitamoto
Appl. Environ. Microbiol., 76, 2556-2561 (2010)
AoSO protein accumulates at the septal pore in response to various stresses in the filamentous fungus Aspergillus oryzae
J. Maruyama, C. S. Escano, K. Kitamoto
Biochem. Biophys. Res. Commun., 391, 868-873 (2010)
Heterologous expression and characterization of an endoglucanase from a symbiotic protist of the lower termite, Reticulitermes speratus
N. Todaka, C. M. Lopez, T. Inoue, K. Saita, J. Maruyama, M. Arioka, K. Kitamoto, T. Kudo, S. Moriya
Appl. Biochem. Biotechnol., 160, 1168-1178 (2010)
Improvement of heterologous protein production in Aspergillus oryzae by RNA interference of α-amylase genes
T. Nemoto, J. Maruyama, K. Kitamoto
Biosci. Biotech. Biochem., 73, 2370-2373 (2009)
Using a new GPI-anchored-protein identification system to mine the protein databases of Aspergillus fumigatus, Aspergillus nidulans, and Aspergillus oryzae
W. Cao, J. Maruyama, K, Kitamoto, K. Sumikoshi, T. Terada, S. Nakamura, K. Shimizu
J. Gen. Appl. Microbiol., 55, 381-393 (2009)
Genetic analysis of glucosidase II beta-subunit in trimming of high-mannose-type glycans
T. Watanabe, K. Totani, I. Matsuo, J. Maruyama, K. Kitamoto, Y. Ito
lycobiology, 19, 834-840 (2009)
Disruption of the Aopex11-1 gene involved in peroxisome proliferation leads to impaired Woronin body formation in Aspergillus oryzae
C. S. Escano, P. R. Juvvadi, F. J. Jin, T. Takahashi, Y. Koyama, S. Yamashita, J. Maruyama, K. Kitamoto
Eukaryot. Cell, 8, 296-305 (2009)
Isolation of Aspergillus oryzae mutants for heterologous protein production from a double proteinase gene disruptant
T. Nemoto, T. Watanabe, Y. Mizogami, J. Maruyama, K. Kitamoto
Appl. Microbiol. Biotechnol., 82, 1105-1114 (2009)
Construction of quintuple protease gene disruptant for heterologous protein production in Aspergillus oryzae
J. Yoon, S. Kimura, J. Maruyama, K. Kitamoto
Appl. Microbiol. Biotechnol., 82, 691-701 (2009)
Codon optimization prevents premature polyadenylation of heterologously-expressed termite cellulases in Aspergillus oryzae and contributes to their improved production
S. Sasaguri, J. Maruyama, S. Moriya, T. Kudo, K. Kitamoto, M. Arioka
J. Gen. Appl. Microbiol., 54, 343-351 (2008)
Multiple gene disruptions by marker recycling with highly efficient gene-targeting background (ΔligD) in Aspergillus oryzae
J. Maruyama, K. Kitamoto
Biotechnol. Lett., 30, 1811-1817 (2008)
Acid-induced sweetness of neoculin is ascribed to its pH-dependent agonistic-antagonistic interaction with human sweet taste receptor
K. Nakajima, Y. Morita, A. Koizumi, T. Asakura, T. Terada, K. Ito, A. Shimizu-Ibuka, J. Maruyama, K. Kitamoto, T. Misaka, K. Abe.
FASEB J., 22, 2323-2330 (2008)
Monitoring Global Gene Expression of Proteases and Improvement of Human Lysozyme Production in the nptB Gene Disruptant of Aspergillus oryzae
S. Kimura, J. Maruyama, M. Takeuchi, K. Kitamoto
Biosci. Biotech. Biochem., 72, 499-502 (2008)
Identification and characterization of an intracellular lectin, calnexin, from Aspergillus oryzae using N-glycan-conjugated beads
T. Watanabe, I. Matsuo, J. Maruyama, K. Kitamoto, Y. Ito
Biosci. Biotech. Biochem., 71, 2688-2696 (2007)
Double disruption of the proteinase genes, tppA and pepE, increases the production level of human lysozyme by Aspergillus oryzae
F. J. Jin, T. Watanabe, P. R. Juvvadi, J. Maruyama, M. Arioka, K. Kitamoto
Appl. Microbiol. Biotechnol., 76, 1059-1068 (2007)
Microbial Production of Sensory-active Miraculin
K. Ito, T. Asakura, Y. Morita, K. Nakajima, A. Koizum, A. Shimizu-Ibuka, K. Masuda, M. Ishiguro, T. Terada, J. Maruyama, K. Kitamoto, T. Misaka, K. Abe
Biochem. Biophys. Res. Commun., 360, 407-411 (2007)
Phosphorylation of the Aspergillus oryzae Woronin body protein, AoHex1, by protein kinase C: evidences for its role in the multimerization and proper localization of the Woronin body protein
P. R. Juvvadi, J. Maruyama, K. Kitamoto
Biochem. J., 405, 533-540 (2007)
Analysis of expressed sequence tags from the fungus Aspergillus oryzae cultured under different conditions
T. Akao, M. Sano, O. Yamada, T. Akeno, K. Fujii, K. Goto, S. Ohashi-Kunihiro, K. Takase, M. Yasukawa-Watanabe, K. Yamaguchi, Y. Kurihara, J. Maruyama, P. R. Juvvadi, A. Tanaka, Y. Hata, Y. Koyama, S. Yamaguchi, N. Kitamoto, K. Gomi, K. Abe, M. Takeuchi, T. Kobayashi, H. Horiuchi, K. Kitamoto, Y. Kashiwagi, M. Machida, O. Akita
DNA Res., 14, 47-57 (2007)
Development of a versatile expression plasmid construction system for Aspergillus oryzae and its application to visualization of mitochondria
Y. Mabashi, T. Kikuma, J. Maruyama, M. Arioka , K. Kitamoto
Biosci. Biotech. Biochem., 70, 1882-1889 (2006)
Extracellular production of neoculin, a sweet-tasting heterodimeric protein with taste-modifying activity, by Aspergillus oryzae
K. Nakajima, T. Asakura, J. Maruyama, Y. Morita, H. Oike, A. Shimizu-Ibuka, T. Misaka, H. Sorimachi, S. Arai, K. Kitamoto, K. Abe
Appl. Environ. Microbiol., 72, 3716-3723 (2006)
Square-plate culture method allows detection of differential gene expression and screening of novel, region-specific genes in Aspergillus oryzae
K. Masai, J. Maruyama, K. Sakamoto, H. Nakajima, O. Akita, K. Kitamoto
Appl. Microbiol. Biotechnol., 71, 881-891 (2006)
Differential distribution of endoplasmic reticulum network as visualized by the BipA-EGFP fusion protein in hyphal compartments across the septum of the filamentous fungus, Aspergillus oryzae
J. Maruyama, S. Kikuchi, K. Kitamoto
Fungal Genet. Biol., 43, 642-654 (2006)
Genome sequencing and analysis of Aspergillus oryzae
M. Machida, K. Asai, M. Sano, T. Tanaka, T. Kumagai, G. Terai, K. Kusumoto, T. Arima, O. Akita, Y. Kashiwagi, K. Abe, K. Gomi, H. Horiuchi, K. Kitamoto, T. Kobayashi, M. Takeuchi, D. W. Denning, J. E. Galagan, W. C. Nierman, J. Yu, D. B. Archer, J. W. Bennett, D. Bhatnagar, T. E. Cleveland, N. D. Fedorova, O. Gotoh, H. Horikawa, A. Hosoyama, M. Ichinomiya, R. Igarashi, K. Iwashita, P. Rao Juvvadi, M. Kato, Y. Kato, T. Kin, A. Kokubun, H. Maeda, N. Maeyama, J. Maruyama, H. Nagasaki, T. Nakajima, K. Oda, K. Okada, I. Paulsen, K. Sakamoto, T. Sawano, M. Takahashi, K. Takase, Y. Terabayashi, J. Wortman, O. Yamada, Y. Yamagata, H. Anazawa, Y. Hata, Y. Koide, T. Komori, Y. Koyama, T. Minetoki, S. Suharnan, A. Tanaka, K. Isono, S. Kuhara, N. Ogasawara and H. Kikuchi
Nature, 438, 1157-1161 (2005)
Development of a modified positive selection medium that allows to isolate Aspergillus oryzae strains cured of the integrated niaD-based plasmid
K. Ishi, T. Watanabe, P. R. Juvvadi, J. Maruyama, K. Kitamoto
Biosci. Biotech. Biochem., 69, 2463-2465 (2005)
Three-dimensional image analysis of plugging at the septal pore by Woronin body during hypotonic shock inducing hyphal tip bursting in Aspergillus oryzae
J. Maruyama, P. R. Juvvadi, K. Ishi, K. Kitamoto
Biochem. Biophys. Res. Commun. 331, 1081-1088 (2005)
Development of Aspergillus oryzaethiA promoter as a tool for molecular biological studies
J. Shoji, J. Maruyama, M. Arioka, K. Kitamoto
FEMS Microbiol. Lett., 244, 41-46 (2005)
Visualizing Nuclear Migration during Conidiophore Development in Aspergillus nidulans and Aspergillus oryzae: Multinucleation of Conidia Occurs through Direct Migration of Plural Nuclei from Phialides and Confers Greater Viability and Early Germination in Aspergillus oryzae
K. Ishi, J. Maruyama, P. R. Juvvadi, H. Nakajima, K. Kitamoto
Biosci. Biotech. Biochem., 69, 747-754 (2005)
Design and synthesis of oligosaccharides that interfere with glycoprotein quality control system
M. A. Arai, I. Matsuo, S. Hagihara, K. Totani, J. Maruyama, K. Kitamoto, Y. Ito
ChemBioChem, 6, 2281-2289 (2005)
Development of a novel quadruple auxotrophic host transformation system by argB gene disruption using adeA gene and exploiting adenine auxotrophy in Aspergillus oryzae
F. J. Jin, J. Maruyama, P. R. Juvvadi, M. Arioka, K. Kitamoto
FEMS Microbiol. Letters, 239, 79-85 (2004)
Adenine auxotrophic mutants of Aspergillus oryzae: development of a noveltransformation system with triple auxotrophic hosts
F. J. Jin, J. Maruyama, P. R. Juvvadi, M. Arioka, K. Kitamoto
Biosci. Biotechnol. Biochem., 68, 656-662 (2004)
Molecular cloning and functional characterization of avaB, a gene encoding Vam6p/Vps39p-like protein in Aspergillus nidulans
M. Oka, J. Maruyama, M. Arioka, H. Nakajima, K. Kitamoto
FEMS Microbiol. Letters, 232, 113-121 (2004)
Effects of protein transport inhibitors on the distribution and secretion of the fusion protein the RntA-EGFP in Aspergillus oryzae
K. Masai, J. Maruyama, H. Nakajima, K. Kitamoto
Biosci. Biotech. Biochem., 68, 1569-1573 (2004)
In vivo visualization of the distribution of a secretory protein in Aspergillus oryzae hyphae using the RntA-EGFP fusion protein
K. Masai, J. Maruyama , H. Nakajima, K. Kitamoto
Biosci. Biotech. Biochem., 67, 455-459 (2003)
Cloning and overexpression of β-N-acetylglucosaminidase encoding gene nagA from Aspergillus oryzae and enzyme-catalyzed synthesis of human milk oligosaccharide
I. Matsuo, S. Kim, Y. Yamamoto, K. Ajisaka, J. Maruyama, H. Nakajima, K. Kitamoto
Biosci. Biotechnol. Biochem., 67, 646-650 (2003)
Novel role of cytoplasmic dynein motor in maintenance of the nuclear number in conidia through organized conidiation in Aspergillus oryzae
J. Maruyama, H. Nakajima, K. Kitamoto
Biochem. Biophys. Res. Commun., 307, 900-906 (2003)
Observation of EGFP-visualized nuclei and distribution of vacuoles in Aspergillus oryzaearpA null mutant
J. Maruyama, H. Nakajima, K. Kitamoto
FEMS Microbiol. Letters, 206, 57-61 (2002)
Visualization of nuclei in Aspergillus oryzae with EGFP and analysis of the number of nuclei in each conidium by FACS
J. Maruyama, H. Nakajima, K. Kitamoto
Biosci. Biotech. Biochem., 65, 1504-1510 (2001)
Production and product quality assessment of human hepatitis B virus Pre-s2 antigen in submerged and solid-state culture conditions of Aspergillus oryzae
J. Maruyama, H. Ohnuma, A. Yoshikawa, H. Kadokura, H. Nakajima, K. Kitamoto
J. Biosci. Bioeng., 90, 118-120 (2000)